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The map label for this gene is mpl [H]

Identifier: 237813866

GI number: 237813866

Start: 3516421

End: 3517935

Strand: Reverse

Name: mpl [H]

Synonym: GBP346_A3644

Alternate gene names: 237813866

Gene position: 3517935-3516421 (Counterclockwise)

Preceding gene: 237813877

Following gene: 237813865

Centisome position: 85.83

GC content: 68.45

Gene sequence:

>1515_bases
TTGCGCGGCGGCGCGAGCGCCGCGCTGCGCATGTTTTCCGGCAACCGGCGCGACGTGCGGCCGGCTGCCGATTTCAACTA
CGCCCACGCGGCGCGAAGCTCTATGCATATCCACATTCTCGGCATCTGCGGCACCTTCATGGGCGGTCTGGCCGTGCTCG
CACGCGCGGCGGGGCATACCGTGACCGGCTGCGACGCGGGCGTCTATCCGCCAATGAGCACGCAACTCGAGGCGCAGGGT
ATCCGGCTGATCGAAGGCTACGGCGCCGAACAGATCGATCTGAAGCCCGATCTGTTCGTGATCGGCAACGTGGTCTCGCG
CGGCAACCCGCTGATGGAGGCGATTCTCGATCGCGGGCTGCCGTATGTATCGGGCCCGCAATGGCTCGGCGAGCATGTGC
TGGCGGGTAAGTGGGTGCTCGCTGTCGCCGGTACGCACGGCAAGACGACGACGAGCTCGATGCTCGCGTGGGTACTGGAA
GACGCCGGCCTGAATCCGGGTTTCCTGATCGGTGGCGTGCCGCTGAATTTCGGCGTGTCCGCACGGTTGACCGATTCGAG
CTTCTTCGTGATCGAGGCGGACGAGTACGACACGGCGTTTTTCGACAAGCGCTCGAAATTCGTCCATTACCGGCCGCGGA
CCGCGATCCTGAACAATCTCGAATTCGATCATGCGGACATCTTCCCGGATCTCGCCGCGATCGAGACGCAATTCCATCAT
CTGGTGCGCACCGTGCCGGGCGTCGGCCGGCTCGTGACGAACGGCCGCGACGACGCGCTCGAGCGCGTGCTGTCGCGCGG
CTGCTGGAGCGACGTCGAGCGCTTCGGTGTCGACGGCGGCTGGCAGGCGTTGCCTGTCGAGAATGGCGTGCCGGTGGACG
GGCGTTTCGCGGTGTATTGGCGCAGCGAGCGCGTCGGCGCCGTCGATTGGCAGGTGCAGGGCGAGCACAATCGGATGAAC
GCGCTCGCGGCGATCGCCGCCGCGCGCCACGTCGGCGTGCCGCCCGCGCAGGCGGCCGCCGCGCTGGCCGCGTTTCGCAA
CGTCAAGCGCCGGATGGAGGTGCGCGGCAGCGTCGACGGGGTCACCGTCTACGACGATTTCGCTCACCACCCCACCGCGA
TCGAAACGACGATTGCCGGCCTTCGTACACGCATCGGCCGCGAAAACACCCGCATCCTCGCGGTGCTGGAGCCGCGGTCG
AACACGATGAAGCTCGGCGTGATGAAGGCGCAACTGCCGGCGAGCCTGGCCGATGCCGATCTCGTGTTCGGCTACGGCGC
GCCGAGCGGCCGCGACGCGCTCGGCTGGAGCCTGCCCGACGCGCTTGCGCCGCTTGGCGGCAAGGCGCGCGCGTTCGACG
ATCTGCATGCGCTCGTGAAGGCCGTGACCGCCTCGGCACGGCCGGGCGACCACGTGCTCGTGATGAGCAACGGCGGCTTC
GGCGGTGTGCACCAGAAGCTGCTCGACGCGCTGTCCGCCCGCGGCGACGCGGCGCCGGCCCGGAGCGGCGCGTGA

Upstream 100 bases:

>100_bases
GCGAGACATGATCCGGCAATCATACTAGATCGGTGCGGAGCGACCCGGCGGTTGCGTCAAGCGTCGTTCGTGCCGGGGGC
GTCAGTTACAATAGCCCTCT

Downstream 100 bases:

>100_bases
TCCTGTATCTGCATGGCTTCCGTTCGTCGCCGCAGTCGTTCAAGGCGCGCGCGCTGGCCGCGCGGCTAGACGAACTCGGC
CGAGCGGACGAATGGCGCTG

Product: UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl- meso-diaminopimelate ligase

Products: NA

Alternate protein names: Murein peptide ligase [H]

Number of amino acids: Translated: 504; Mature: 504

Protein sequence:

>504_residues
MRGGASAALRMFSGNRRDVRPAADFNYAHAARSSMHIHILGICGTFMGGLAVLARAAGHTVTGCDAGVYPPMSTQLEAQG
IRLIEGYGAEQIDLKPDLFVIGNVVSRGNPLMEAILDRGLPYVSGPQWLGEHVLAGKWVLAVAGTHGKTTTSSMLAWVLE
DAGLNPGFLIGGVPLNFGVSARLTDSSFFVIEADEYDTAFFDKRSKFVHYRPRTAILNNLEFDHADIFPDLAAIETQFHH
LVRTVPGVGRLVTNGRDDALERVLSRGCWSDVERFGVDGGWQALPVENGVPVDGRFAVYWRSERVGAVDWQVQGEHNRMN
ALAAIAAARHVGVPPAQAAAALAAFRNVKRRMEVRGSVDGVTVYDDFAHHPTAIETTIAGLRTRIGRENTRILAVLEPRS
NTMKLGVMKAQLPASLADADLVFGYGAPSGRDALGWSLPDALAPLGGKARAFDDLHALVKAVTASARPGDHVLVMSNGGF
GGVHQKLLDALSARGDAAPARSGA

Sequences:

>Translated_504_residues
MRGGASAALRMFSGNRRDVRPAADFNYAHAARSSMHIHILGICGTFMGGLAVLARAAGHTVTGCDAGVYPPMSTQLEAQG
IRLIEGYGAEQIDLKPDLFVIGNVVSRGNPLMEAILDRGLPYVSGPQWLGEHVLAGKWVLAVAGTHGKTTTSSMLAWVLE
DAGLNPGFLIGGVPLNFGVSARLTDSSFFVIEADEYDTAFFDKRSKFVHYRPRTAILNNLEFDHADIFPDLAAIETQFHH
LVRTVPGVGRLVTNGRDDALERVLSRGCWSDVERFGVDGGWQALPVENGVPVDGRFAVYWRSERVGAVDWQVQGEHNRMN
ALAAIAAARHVGVPPAQAAAALAAFRNVKRRMEVRGSVDGVTVYDDFAHHPTAIETTIAGLRTRIGRENTRILAVLEPRS
NTMKLGVMKAQLPASLADADLVFGYGAPSGRDALGWSLPDALAPLGGKARAFDDLHALVKAVTASARPGDHVLVMSNGGF
GGVHQKLLDALSARGDAAPARSGA
>Mature_504_residues
MRGGASAALRMFSGNRRDVRPAADFNYAHAARSSMHIHILGICGTFMGGLAVLARAAGHTVTGCDAGVYPPMSTQLEAQG
IRLIEGYGAEQIDLKPDLFVIGNVVSRGNPLMEAILDRGLPYVSGPQWLGEHVLAGKWVLAVAGTHGKTTTSSMLAWVLE
DAGLNPGFLIGGVPLNFGVSARLTDSSFFVIEADEYDTAFFDKRSKFVHYRPRTAILNNLEFDHADIFPDLAAIETQFHH
LVRTVPGVGRLVTNGRDDALERVLSRGCWSDVERFGVDGGWQALPVENGVPVDGRFAVYWRSERVGAVDWQVQGEHNRMN
ALAAIAAARHVGVPPAQAAAALAAFRNVKRRMEVRGSVDGVTVYDDFAHHPTAIETTIAGLRTRIGRENTRILAVLEPRS
NTMKLGVMKAQLPASLADADLVFGYGAPSGRDALGWSLPDALAPLGGKARAFDDLHALVKAVTASARPGDHVLVMSNGGF
GGVHQKLLDALSARGDAAPARSGA

Specific function: Reutilizes the intact tripeptide L-alanyl-gamma-D- glutamyl-meso-diaminopimelate by linking it to UDP-N-acetylmuramic acid [H]

COG id: COG0773

COG function: function code M; UDP-N-acetylmuramate-alanine ligase

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1790680, Length=464, Percent_Identity=58.8362068965517, Blast_Score=543, Evalue=1e-155,
Organism=Escherichia coli, GI1786279, Length=285, Percent_Identity=30.1754385964912, Blast_Score=107, Evalue=3e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005757
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: 6.3.2.- [C]

Molecular weight: Translated: 53803; Mature: 53803

Theoretical pI: Translated: 7.55; Mature: 7.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRGGASAALRMFSGNRRDVRPAADFNYAHAARSSMHIHILGICGTFMGGLAVLARAAGHT
CCCCHHHHHHHHCCCCCCCCCCCCCCHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHCCCE
VTGCDAGVYPPMSTQLEAQGIRLIEGYGAEQIDLKPDLFVIGNVVSRGNPLMEAILDRGL
EECCCCCCCCCCCCCCCCCCEEEEECCCCCEECCCCCEEEEECHHHCCCHHHHHHHHCCC
PYVSGPQWLGEHVLAGKWVLAVAGTHGKTTTSSMLAWVLEDAGLNPGFLIGGVPLNFGVS
CCCCCHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCCCEEEECCCCCCCCE
ARLTDSSFFVIEADEYDTAFFDKRSKFVHYRPRTAILNNLEFDHADIFPDLAAIETQFHH
EEEECCEEEEEECCCCCCHHHHCCCCEEEECCHHHHHCCCCCCHHHHCCHHHHHHHHHHH
LVRTVPGVGRLVTNGRDDALERVLSRGCWSDVERFGVDGGWQALPVENGVPVDGRFAVYW
HHHHCCCCCHHHCCCCHHHHHHHHHCCCHHHHHHHCCCCCCEEEECCCCCCCCCEEEEEE
RSERVGAVDWQVQGEHNRMNALAAIAAARHVGVPPAQAAAALAAFRNVKRRMEVRGSVDG
ECCCCCEEEEEEECCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCC
VTVYDDFAHHPTAIETTIAGLRTRIGRENTRILAVLEPRSNTMKLGVMKAQLPASLADAD
EEEECCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCCEEEEEEEHHCCCCCCCCC
LVFGYGAPSGRDALGWSLPDALAPLGGKARAFDDLHALVKAVTASARPGDHVLVMSNGGF
EEEECCCCCCCCCCCCCCCHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCC
GGVHQKLLDALSARGDAAPARSGA
CHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MRGGASAALRMFSGNRRDVRPAADFNYAHAARSSMHIHILGICGTFMGGLAVLARAAGHT
CCCCHHHHHHHHCCCCCCCCCCCCCCHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHCCCE
VTGCDAGVYPPMSTQLEAQGIRLIEGYGAEQIDLKPDLFVIGNVVSRGNPLMEAILDRGL
EECCCCCCCCCCCCCCCCCCEEEEECCCCCEECCCCCEEEEECHHHCCCHHHHHHHHCCC
PYVSGPQWLGEHVLAGKWVLAVAGTHGKTTTSSMLAWVLEDAGLNPGFLIGGVPLNFGVS
CCCCCHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCCCEEEECCCCCCCCE
ARLTDSSFFVIEADEYDTAFFDKRSKFVHYRPRTAILNNLEFDHADIFPDLAAIETQFHH
EEEECCEEEEEECCCCCCHHHHCCCCEEEECCHHHHHCCCCCCHHHHCCHHHHHHHHHHH
LVRTVPGVGRLVTNGRDDALERVLSRGCWSDVERFGVDGGWQALPVENGVPVDGRFAVYW
HHHHCCCCCHHHCCCCHHHHHHHHHCCCHHHHHHHCCCCCCEEEECCCCCCCCCEEEEEE
RSERVGAVDWQVQGEHNRMNALAAIAAARHVGVPPAQAAAALAAFRNVKRRMEVRGSVDG
ECCCCCEEEEEEECCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCC
VTVYDDFAHHPTAIETTIAGLRTRIGRENTRILAVLEPRSNTMKLGVMKAQLPASLADAD
EEEECCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCCEEEEEEEHHCCCCCCCCC
LVFGYGAPSGRDALGWSLPDALAPLGGKARAFDDLHALVKAVTASARPGDHVLVMSNGGF
EEEECCCCCCCCCCCCCCCHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCC
GGVHQKLLDALSARGDAAPARSGA
CHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7610040; 9278503; 2843822; 7984428; 8808921 [H]