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The map label for this gene is dctA2 [H]

Identifier: 237813825

GI number: 237813825

Start: 3479815

End: 3481137

Strand: Reverse

Name: dctA2 [H]

Synonym: GBP346_A3603

Alternate gene names: 237813825

Gene position: 3481137-3479815 (Counterclockwise)

Preceding gene: 237813828

Following gene: 237813824

Centisome position: 84.94

GC content: 65.08

Gene sequence:

>1323_bases
GTGTCGAAGTTTCTCAACTCGCTGTTTGGTCGGGTGGTCATCGCGCTGTTGATCGGCGTGGCGCTCGGTGCCTTTTTTCC
GCACTTCGCCCAGTCGCTGCGACCGTTGGGTGACGGGTTCCTGAAGCTGATCAAGATGGTGATCGGGCCGATCGTGTTCT
GTGTCGTCGTGAGCGGCATGGCGAACGCGGGCGATCTGAAGAAGGTCGGCCGCGTCGGCCTGAAGGCCGTCATCTACTTC
GAGGCGATGACGACGCTCGCGCTCGGCATCGGCCTCGTGCTCGCGTACCTGACGAAGCCGGGCGTCGGGATGAACGTCGA
TCTGCATACGCTCGATCCCGCGTCGCTCGCCGATTACGCGAAGAACGCGCAGAGCCTGAAGGATACGGCGGGCTATCTGC
TGAAGATCATTCCCGAGACCGCGTTCGACGCGTTCGCGAAGGGCGACATCCTGCAGATCCTCGTGTTCTCGGTGCTGTTC
GGCTCCGCGCTGTCGCTGCTCGGCGAGAAGGCGAAGCGTGTGAATACGCTGATCGACGAATTCGCGCAGGTGTTCTTCCG
CGTGATGGGCTTCATCATCAAGCTCGCGCCGCTCGGCGTGCTCGGCGCGATCGCGTTCACGACCGGCAAGTACGGCGTCG
CGTCGCTCAAGCAGCTGGGTCTTCTCGTCATCGTGTTCTACGCGAGCTGCATCGCGTTCGTCGTGATCGTGCTCGGCGCG
GTGATGAAGCTCGCGGGCTTCTCGGTGTTCAAGCTGATCCGCTACCTGCGCGAGGAACTCCTGATCGTGCTCGGCACCGC
ATCGTCGGACGCCGTGCTGCCGCAAGTGATGCGCAAGCTCGAATGGATGGGGGTGAAGGATTCGACGGTGGGCCTCGTGA
TTCCCACCGGCTACTCGTTCAACCTCGACGGCTTCTCGATCTACCTGACGCTCGCGGTGCTCTTCATCGCGCAGGCGACC
AACACGCCGCTGTCGGTGCACGATCTGATCGTCGTGCTGCTCGTGTCGCTCGTCACGTCGAAGGGCGCGCACGGCATCCC
GGGCTCGGCGATCGTGATTCTCGCCGCGACGCTGTCGGCGATCCCCGCGCTGCCCGTGCTCGGCCTCGTGCTGATCCTGC
CCGTCGACTGGTTCGTCGGCATCGCGCGCGCGCTCACGAACCTGCTCGGCAACTGCGTGGCGACGATCGTCGTCGCGGTA
TGGGAGCACGACATCGACCGCGCCCGCGCGCAGCGCGTGCTGAACGAAGAGCTGCGCTACGTGCCGGCGGGCGACGAGAG
CCCGCGCGGCGCGATCTCGGACGGCGGCGCGCACGCGCTCTGA

Upstream 100 bases:

>100_bases
CTTGACTCGACTATATCGGCTTCCTACTATCGTCCATAACAGTACTGGTCGGACCAGTATGAACAGAGGAAATCCGCAAC
CGTTGTGACGGGAGAACGCC

Downstream 100 bases:

>100_bases
CGCGGCCCCGCCCGGCATCATCGCGCGGCCGGCGCTCAGCTTCGCGCCGGCGCTTCGTTTTTCAGGCTGGCGCAATGGCC
GCCGATTTTTCCGCCGGTTT

Product: C4-dicarboxylate transporter DctA

Products: orotate [Cytoplasm]; fumarate [Cytoplasm]; malate [Cytoplasm]; Na (I) [Cytoplasm]; succinate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 440; Mature: 439

Protein sequence:

>440_residues
MSKFLNSLFGRVVIALLIGVALGAFFPHFAQSLRPLGDGFLKLIKMVIGPIVFCVVVSGMANAGDLKKVGRVGLKAVIYF
EAMTTLALGIGLVLAYLTKPGVGMNVDLHTLDPASLADYAKNAQSLKDTAGYLLKIIPETAFDAFAKGDILQILVFSVLF
GSALSLLGEKAKRVNTLIDEFAQVFFRVMGFIIKLAPLGVLGAIAFTTGKYGVASLKQLGLLVIVFYASCIAFVVIVLGA
VMKLAGFSVFKLIRYLREELLIVLGTASSDAVLPQVMRKLEWMGVKDSTVGLVIPTGYSFNLDGFSIYLTLAVLFIAQAT
NTPLSVHDLIVVLLVSLVTSKGAHGIPGSAIVILAATLSAIPALPVLGLVLILPVDWFVGIARALTNLLGNCVATIVVAV
WEHDIDRARAQRVLNEELRYVPAGDESPRGAISDGGAHAL

Sequences:

>Translated_440_residues
MSKFLNSLFGRVVIALLIGVALGAFFPHFAQSLRPLGDGFLKLIKMVIGPIVFCVVVSGMANAGDLKKVGRVGLKAVIYF
EAMTTLALGIGLVLAYLTKPGVGMNVDLHTLDPASLADYAKNAQSLKDTAGYLLKIIPETAFDAFAKGDILQILVFSVLF
GSALSLLGEKAKRVNTLIDEFAQVFFRVMGFIIKLAPLGVLGAIAFTTGKYGVASLKQLGLLVIVFYASCIAFVVIVLGA
VMKLAGFSVFKLIRYLREELLIVLGTASSDAVLPQVMRKLEWMGVKDSTVGLVIPTGYSFNLDGFSIYLTLAVLFIAQAT
NTPLSVHDLIVVLLVSLVTSKGAHGIPGSAIVILAATLSAIPALPVLGLVLILPVDWFVGIARALTNLLGNCVATIVVAV
WEHDIDRARAQRVLNEELRYVPAGDESPRGAISDGGAHAL
>Mature_439_residues
SKFLNSLFGRVVIALLIGVALGAFFPHFAQSLRPLGDGFLKLIKMVIGPIVFCVVVSGMANAGDLKKVGRVGLKAVIYFE
AMTTLALGIGLVLAYLTKPGVGMNVDLHTLDPASLADYAKNAQSLKDTAGYLLKIIPETAFDAFAKGDILQILVFSVLFG
SALSLLGEKAKRVNTLIDEFAQVFFRVMGFIIKLAPLGVLGAIAFTTGKYGVASLKQLGLLVIVFYASCIAFVVIVLGAV
MKLAGFSVFKLIRYLREELLIVLGTASSDAVLPQVMRKLEWMGVKDSTVGLVIPTGYSFNLDGFSIYLTLAVLFIAQATN
TPLSVHDLIVVLLVSLVTSKGAHGIPGSAIVILAATLSAIPALPVLGLVLILPVDWFVGIARALTNLLGNCVATIVVAVW
EHDIDRARAQRVLNEELRYVPAGDESPRGAISDGGAHAL

Specific function: Responsible for the transport of dicarboxylates such as succinate, fumarate, and malate from the periplasm across the membrane [H]

COG id: COG1301

COG function: function code C; Na+/H+-dicarboxylate symporters

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family [H]

Homologues:

Organism=Homo sapiens, GI169790839, Length=465, Percent_Identity=26.6666666666667, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI4827012, Length=266, Percent_Identity=31.9548872180451, Blast_Score=111, Evalue=2e-24,
Organism=Homo sapiens, GI40254478, Length=445, Percent_Identity=24.9438202247191, Blast_Score=105, Evalue=1e-22,
Organism=Homo sapiens, GI21314632, Length=431, Percent_Identity=25.754060324826, Blast_Score=102, Evalue=6e-22,
Organism=Homo sapiens, GI66773030, Length=412, Percent_Identity=25, Blast_Score=100, Evalue=5e-21,
Organism=Homo sapiens, GI5032093, Length=407, Percent_Identity=26.044226044226, Blast_Score=96, Evalue=5e-20,
Organism=Homo sapiens, GI223468566, Length=282, Percent_Identity=28.0141843971631, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI223468564, Length=282, Percent_Identity=28.0141843971631, Blast_Score=91, Evalue=3e-18,
Organism=Homo sapiens, GI262359914, Length=391, Percent_Identity=25.3196930946292, Blast_Score=87, Evalue=3e-17,
Organism=Homo sapiens, GI194239697, Length=271, Percent_Identity=26.9372693726937, Blast_Score=81, Evalue=2e-15,
Organism=Homo sapiens, GI301601644, Length=191, Percent_Identity=28.2722513089005, Blast_Score=79, Evalue=7e-15,
Organism=Escherichia coli, GI1789947, Length=416, Percent_Identity=52.4038461538462, Blast_Score=436, Evalue=1e-123,
Organism=Escherichia coli, GI1790514, Length=417, Percent_Identity=38.1294964028777, Blast_Score=283, Evalue=2e-77,
Organism=Escherichia coli, GI1788024, Length=406, Percent_Identity=22.6600985221675, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1789473, Length=331, Percent_Identity=23.2628398791541, Blast_Score=64, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI71996953, Length=472, Percent_Identity=27.1186440677966, Blast_Score=119, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI17537407, Length=385, Percent_Identity=27.012987012987, Blast_Score=116, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI193206654, Length=451, Percent_Identity=25.4988913525499, Blast_Score=112, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI193206505, Length=482, Percent_Identity=26.3485477178423, Blast_Score=106, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI71983099, Length=449, Percent_Identity=24.7216035634744, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI71983106, Length=449, Percent_Identity=24.7216035634744, Blast_Score=100, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17541374, Length=400, Percent_Identity=25, Blast_Score=99, Evalue=6e-21,
Organism=Drosophila melanogaster, GI24583025, Length=436, Percent_Identity=24.0825688073394, Blast_Score=109, Evalue=3e-24,
Organism=Drosophila melanogaster, GI17137668, Length=436, Percent_Identity=24.0825688073394, Blast_Score=109, Evalue=3e-24,
Organism=Drosophila melanogaster, GI24583023, Length=436, Percent_Identity=24.0825688073394, Blast_Score=109, Evalue=3e-24,
Organism=Drosophila melanogaster, GI281360483, Length=453, Percent_Identity=27.3730684326711, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI17137666, Length=453, Percent_Identity=27.3730684326711, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI281360481, Length=453, Percent_Identity=27.3730684326711, Blast_Score=100, Evalue=3e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001991
- InterPro:   IPR018107 [H]

Pfam domain/function: PF00375 SDF [H]

EC number: NA

Molecular weight: Translated: 46680; Mature: 46549

Theoretical pI: Translated: 9.40; Mature: 9.40

Prosite motif: PS00713 NA_DICARBOXYL_SYMP_1 ; PS00714 NA_DICARBOXYL_SYMP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SKFLNSLFGRVVIALLIGVALGAFFPHFAQSLRPLGDGFLKLIKMVIGPIVFCVVVSGM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC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CCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: orotate [Periplasm]; fumarate [Periplasm]; malate [Periplasm]; Na (I) [Periplasm]; succinate [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + orotate [Periplasm] = Na (I) [Cytoplasm] + orotate [Cytoplasm] Na (I) [Periplasm] + fumarate [Periplasm] = Na (I) [Cytoplasm] + fumarate [Cytoplasm] Na (I) [Periplasm] + malate [Periplasm] = Na (I) [Cytoplasm] + malate [Cytoplasm] Na

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]