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The map label for this gene is rlmL [H]

Identifier: 237813743

GI number: 237813743

Start: 3407592

End: 3408884

Strand: Direct

Name: rlmL [H]

Synonym: GBP346_A3520

Alternate gene names: 237813743

Gene position: 3407592-3408884 (Clockwise)

Preceding gene: 237813742

Following gene: 237813744

Centisome position: 83.14

GC content: 71.46

Gene sequence:

>1293_bases
ATGTCCTCGCCGACCCTATTCGACTTCTTCGCTCCCTGCCCGCGCGGGCTCGAGGCCGCGCTCGCCGCCGAGCTCGCGCA
GATCGCCGCGCGCCACGCGATCGCCCCGTACGACGCGGGCGCGCAAGTGCCGGGCGGCGTGCATTTCCGTGGCGCCTGGG
CGGCCGGCATGGCCGCCAACCTGCATTCGCGGATCGCGAGCCGCGTGCTGCTGAAGATCGCGCACCGCCCGTACCGCAAC
GAGCAGGACGTCTACGCGCTCGCGCTCGAGCAGCCGTGGGAGCGCTGGTTCGCATCGACGCAGACGCTGCGCGTGGACAT
CACCGCGATCAAGTCGCCGCTCAAGAGCCTGGAATTCGCGACGCTGCGCGTGAAGGACGCGATCTGCGACCGCCTGCGCG
AGAAGACGGGCGCCCGCCCGAGCATCGACACCGCCCTGCCCGACGTGCGCGTGTTCGCGTTCCTGACGGCGGGCGACTGC
ACGCTATATCTCGACACGTCCGGCGAGCCGCTCTTCAAGCGCGGCTGGCGTCTCGACAAGGGCGCGGCGCCGCTGCGCGA
GAATCTCGCGGCGGGCATCCTGCGCCTGGCCGGCTGGACGCCGGGCACGCCGCTCTACGATCCGATGTGCGGCAGCGGCA
CGTTCGTCGCGGAAGCAGCGCAGATCGCGCTCGGCGTCGCGCCGGGCGTCGAGCGCCGCTTCGGCTTCGAGAAGCTCAAG
CAATACGACATCACCGCGTGGCAGACGCTGAAGGTCGCCGCGATGGACGCGAAGCGCGCGGCGCGCGGCAAGCGCGACGA
TCTGCTGATCTTCGGCAGCGACATCTCGGGCGACATGCTCGAGAAGGCGCGCGCGAACCTCGAGCGCGCGGGCGTGCCGT
CGCTGTGGCTCAAGCAGATCGACGCGCGCAACATGACGCCGCCCGCCGCCGCGCCCGGCGTGATCGTCACGAACCCGCCG
TACGGCGAGCGGATCGAGGTGCGCGGCCGCGGCCCGCGCGGCGACGTGCGCGAGACGGGCAAGAACCGCGGCGGCGACGA
AGCCTTCCGCCGCACGCACGCCGATTCGCCCGACAGCGAATTCTTCCGCGCGCTCGGCGATGCGCTCAAGCAGCGCTTCG
CCGGCTGGCAGGCGTTCATGCTGAGCTCGGACCGCACGCTGCCGGGCCAGTTGCGACTGCGCGAATCGGCGAAGACGCCG
TTGTTCAACGGCGCGCTCGAGTGCCGGCTGTTCCGTTTCGATCTGATCGCGGGCAGCATCCGGCAACGCCCGGCAGCGGC
CGACGGCGAGTAA

Upstream 100 bases:

>100_bases
CAAGACGTGGCGGCACGGCGGCCTCACCGCGTGACGACGTGGCGGCCGCCGCGCCCACCCCGCGTACAATGGCGTACTTT
TGCACGCGACAGCCCGCCTT

Downstream 100 bases:

>100_bases
CGCGACGCGCGCTCGTCGCGTATGTGCCGCGCATGCGCCACTTCGTGAGCGGGGCGCGGGAACTTCGCGGCCGCGCGAGC
GTCCATGCGCATCGCGCATC

Product: thump domain protein

Products: NA

Alternate protein names: 23S rRNA m2G2445 methyltransferase; rRNA (guanine-N(2)-)-methyltransferase rlmL [H]

Number of amino acids: Translated: 430; Mature: 429

Protein sequence:

>430_residues
MSSPTLFDFFAPCPRGLEAALAAELAQIAARHAIAPYDAGAQVPGGVHFRGAWAAGMAANLHSRIASRVLLKIAHRPYRN
EQDVYALALEQPWERWFASTQTLRVDITAIKSPLKSLEFATLRVKDAICDRLREKTGARPSIDTALPDVRVFAFLTAGDC
TLYLDTSGEPLFKRGWRLDKGAAPLRENLAAGILRLAGWTPGTPLYDPMCGSGTFVAEAAQIALGVAPGVERRFGFEKLK
QYDITAWQTLKVAAMDAKRAARGKRDDLLIFGSDISGDMLEKARANLERAGVPSLWLKQIDARNMTPPAAAPGVIVTNPP
YGERIEVRGRGPRGDVRETGKNRGGDEAFRRTHADSPDSEFFRALGDALKQRFAGWQAFMLSSDRTLPGQLRLRESAKTP
LFNGALECRLFRFDLIAGSIRQRPAAADGE

Sequences:

>Translated_430_residues
MSSPTLFDFFAPCPRGLEAALAAELAQIAARHAIAPYDAGAQVPGGVHFRGAWAAGMAANLHSRIASRVLLKIAHRPYRN
EQDVYALALEQPWERWFASTQTLRVDITAIKSPLKSLEFATLRVKDAICDRLREKTGARPSIDTALPDVRVFAFLTAGDC
TLYLDTSGEPLFKRGWRLDKGAAPLRENLAAGILRLAGWTPGTPLYDPMCGSGTFVAEAAQIALGVAPGVERRFGFEKLK
QYDITAWQTLKVAAMDAKRAARGKRDDLLIFGSDISGDMLEKARANLERAGVPSLWLKQIDARNMTPPAAAPGVIVTNPP
YGERIEVRGRGPRGDVRETGKNRGGDEAFRRTHADSPDSEFFRALGDALKQRFAGWQAFMLSSDRTLPGQLRLRESAKTP
LFNGALECRLFRFDLIAGSIRQRPAAADGE
>Mature_429_residues
SSPTLFDFFAPCPRGLEAALAAELAQIAARHAIAPYDAGAQVPGGVHFRGAWAAGMAANLHSRIASRVLLKIAHRPYRNE
QDVYALALEQPWERWFASTQTLRVDITAIKSPLKSLEFATLRVKDAICDRLREKTGARPSIDTALPDVRVFAFLTAGDCT
LYLDTSGEPLFKRGWRLDKGAAPLRENLAAGILRLAGWTPGTPLYDPMCGSGTFVAEAAQIALGVAPGVERRFGFEKLKQ
YDITAWQTLKVAAMDAKRAARGKRDDLLIFGSDISGDMLEKARANLERAGVPSLWLKQIDARNMTPPAAAPGVIVTNPPY
GERIEVRGRGPRGDVRETGKNRGGDEAFRRTHADSPDSEFFRALGDALKQRFAGWQAFMLSSDRTLPGQLRLRESAKTPL
FNGALECRLFRFDLIAGSIRQRPAAADGE

Specific function: Specifically methylates the guanosine in position 2445 (m2G2445) of 23S rRNA [H]

COG id: COG0116

COG function: function code L; Predicted N6-adenine-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 THUMP domain [H]

Homologues:

Organism=Escherichia coli, GI1787181, Length=387, Percent_Identity=31.5245478036176, Blast_Score=192, Evalue=4e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR000241
- InterPro:   IPR017244
- InterPro:   IPR019614
- InterPro:   IPR004114 [H]

Pfam domain/function: PF10672 Methyltrans_SAM; PF02926 THUMP; PF01170 UPF0020 [H]

EC number: =2.1.1.173 [H]

Molecular weight: Translated: 47043; Mature: 46912

Theoretical pI: Translated: 9.96; Mature: 9.96

Prosite motif: PS51165 THUMP ; PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSPTLFDFFAPCPRGLEAALAAELAQIAARHAIAPYDAGAQVPGGVHFRGAWAAGMAAN
CCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEECCHHHHHHHHH
LHSRIASRVLLKIAHRPYRNEQDVYALALEQPWERWFASTQTLRVDITAIKSPLKSLEFA
HHHHHHHHHHHHHHCCCCCCCCCEEEEEHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHH
TLRVKDAICDRLREKTGARPSIDTALPDVRVFAFLTAGDCTLYLDTSGEPLFKRGWRLDK
HHHHHHHHHHHHHHHHCCCCCHHHCCCCEEEEEEEEECCEEEEEECCCCHHHHCCCCCCC
GAAPLRENLAAGILRLAGWTPGTPLYDPMCGSGTFVAEAAQIALGVAPGVERRFGFEKLK
CCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHCHHHHH
QYDITAWQTLKVAAMDAKRAARGKRDDLLIFGSDISGDMLEKARANLERAGVPSLWLKQI
HHCCCHHHEEEHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCHHHHHHH
DARNMTPPAAAPGVIVTNPPYGERIEVRGRGPRGDVRETGKNRGGDEAFRRTHADSPDSE
CCCCCCCCCCCCCEEECCCCCCCEEEEECCCCCCCHHHHCCCCCCHHHHHHHCCCCCHHH
FFRALGDALKQRFAGWQAFMLSSDRTLPGQLRLRESAKTPLFNGALECRLFRFDLIAGSI
HHHHHHHHHHHHHCCCEEEEECCCCCCCCHHEEHHHCCCCCCCCCHHEEEEEHHHHHHHH
RQRPAAADGE
HCCCCCCCCC
>Mature Secondary Structure 
SSPTLFDFFAPCPRGLEAALAAELAQIAARHAIAPYDAGAQVPGGVHFRGAWAAGMAAN
CCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEECCHHHHHHHHH
LHSRIASRVLLKIAHRPYRNEQDVYALALEQPWERWFASTQTLRVDITAIKSPLKSLEFA
HHHHHHHHHHHHHHCCCCCCCCCEEEEEHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHH
TLRVKDAICDRLREKTGARPSIDTALPDVRVFAFLTAGDCTLYLDTSGEPLFKRGWRLDK
HHHHHHHHHHHHHHHHCCCCCHHHCCCCEEEEEEEEECCEEEEEECCCCHHHHCCCCCCC
GAAPLRENLAAGILRLAGWTPGTPLYDPMCGSGTFVAEAAQIALGVAPGVERRFGFEKLK
CCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHCHHHHH
QYDITAWQTLKVAAMDAKRAARGKRDDLLIFGSDISGDMLEKARANLERAGVPSLWLKQI
HHCCCHHHEEEHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCHHHHHHH
DARNMTPPAAAPGVIVTNPPYGERIEVRGRGPRGDVRETGKNRGGDEAFRRTHADSPDSE
CCCCCCCCCCCCCEEECCCCCCCEEEEECCCCCCCHHHHCCCCCCHHHHHHHCCCCCHHH
FFRALGDALKQRFAGWQAFMLSSDRTLPGQLRLRESAKTPLFNGALECRLFRFDLIAGSI
HHHHHHHHHHHHHCCCEEEEECCCCCCCCHHEEHHHCCCCCCCCCHHEEEEEHHHHHHHH
RQRPAAADGE
HCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA