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The map label for this gene is tgt [H]

Identifier: 237813726

GI number: 237813726

Start: 3391713

End: 3392897

Strand: Direct

Name: tgt [H]

Synonym: GBP346_A3503

Alternate gene names: 237813726

Gene position: 3391713-3392897 (Clockwise)

Preceding gene: 237813725

Following gene: 237813727

Centisome position: 82.75

GC content: 66.75

Gene sequence:

>1185_bases
ATGACGACCGAAGGTCCATCGCACGATACCTCCGCGGGCATTCGCCCGCACGGCGGCCTGAAATTCGAGCTGCTGACGAC
CGACGGCCGCGCGCGGCGCGGCCGCGTGACGCTCAATCACGGCGTCGTCGAGACGCCGATCTTCATGCCGGTCGGCACGT
ACGGCACCGTGAAGGCCGTGCAGCCGCGCGAACTCGACGAAATGCGCGCGCAGATCATCCTCGGCAACACGTTCCACCTG
TGGCTGCGCCCCGGCCTCGAGACGATCGGCGCGCACGGCGGGCTGCACCGCTTCATGGGCTGGAACAAGCCGATCCTCAC
CGATTCGGGCGGCTTCCAGGTGTTCTCGCTCGGCGATCTGCGCAAGATCACCGAGGAAGGCGTCACGTTCGCGTCGCCGA
TCAACGGCGATCGGCTGTTCCTGTCGCCGGAAGTGTCGATGCAGATCCAGAAGGCGCTGAATTCCGACATCGTGATGCAG
TTCGACGAATGCACGCCGTACGCGACGAACGGCGTGCCGACGACGCACCGGGAAGCGGCCGAATCGATGCGGATGTCGCT
GCGCTGGGCGAAGCGCTCGCTCGACGAATTCGAGCGCCTCGGCAACCCGAACGCGCTGTTCGGCATCGTGCAGGGCGGGA
TGTACGAGGACCTGCGCGACGAATCGCTCGCTGGGCTCTCGGCGCTCGGCTTCCACGGCCTCGCGATCGGCGGGCTGTCG
GTCGGCGAGCCGAAGGAGGACATGATGCGCGTGCTCGAGCACGTCGCGCCGCGCCTGCCCGCCGACAAGCCGCACTACCT
GATGGGCGTGGGCACGCCGGAGGATCTCGTCGCGGGCGTCGCGGCGGGCGTCGACATGTTCGACTGCGTGATGCCGACCC
GCAACGCGCGCAACGGCTGGCTCTTCACGCGCTTCGGCGACGTGAAGATCCGCAACGCGACGCACAAGAATTCGCTCAAG
CCGCTCGACGAGACCTGCGGCTGCTATACGTGCCGGAATTTCTCGCGCGGCTACCTGCATCATCTGCACCGCGTCGGCGA
GATTCTCGGCGCGCAGTTGAACACGATCCACAACCTGCACTACTACCTCGATCTGATGCGCGAGATCCGCGAGGCGATCG
AGACGCACACGTTCGAGGCGTTCCAAAAGCGCTTCGCGCAGGACCGCGCGCGCGGCGTCGACTGA

Upstream 100 bases:

>100_bases
CGCGAGCCGCGCTTCACTTTTTTCCTTCGCGGCAAGCCGACGGCTCGCCGCGCCTTCAACATGCGCCGGACTGTATTCCG
GTGGCAAGGAGTCCAACCAG

Downstream 100 bases:

>100_bases
CGCCGCGCGGCCGCGCCGGCATCGGCATCGGCATCGGCATCGGCATTGGCGACGCCGCGCTCGCGCGCCAATCGCGCGGG
CAATGCCGCCGCGCCGCAGC

Product: queuine tRNA-ribosyltransferase

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase [H]

Number of amino acids: Translated: 394; Mature: 393

Protein sequence:

>394_residues
MTTEGPSHDTSAGIRPHGGLKFELLTTDGRARRGRVTLNHGVVETPIFMPVGTYGTVKAVQPRELDEMRAQIILGNTFHL
WLRPGLETIGAHGGLHRFMGWNKPILTDSGGFQVFSLGDLRKITEEGVTFASPINGDRLFLSPEVSMQIQKALNSDIVMQ
FDECTPYATNGVPTTHREAAESMRMSLRWAKRSLDEFERLGNPNALFGIVQGGMYEDLRDESLAGLSALGFHGLAIGGLS
VGEPKEDMMRVLEHVAPRLPADKPHYLMGVGTPEDLVAGVAAGVDMFDCVMPTRNARNGWLFTRFGDVKIRNATHKNSLK
PLDETCGCYTCRNFSRGYLHHLHRVGEILGAQLNTIHNLHYYLDLMREIREAIETHTFEAFQKRFAQDRARGVD

Sequences:

>Translated_394_residues
MTTEGPSHDTSAGIRPHGGLKFELLTTDGRARRGRVTLNHGVVETPIFMPVGTYGTVKAVQPRELDEMRAQIILGNTFHL
WLRPGLETIGAHGGLHRFMGWNKPILTDSGGFQVFSLGDLRKITEEGVTFASPINGDRLFLSPEVSMQIQKALNSDIVMQ
FDECTPYATNGVPTTHREAAESMRMSLRWAKRSLDEFERLGNPNALFGIVQGGMYEDLRDESLAGLSALGFHGLAIGGLS
VGEPKEDMMRVLEHVAPRLPADKPHYLMGVGTPEDLVAGVAAGVDMFDCVMPTRNARNGWLFTRFGDVKIRNATHKNSLK
PLDETCGCYTCRNFSRGYLHHLHRVGEILGAQLNTIHNLHYYLDLMREIREAIETHTFEAFQKRFAQDRARGVD
>Mature_393_residues
TTEGPSHDTSAGIRPHGGLKFELLTTDGRARRGRVTLNHGVVETPIFMPVGTYGTVKAVQPRELDEMRAQIILGNTFHLW
LRPGLETIGAHGGLHRFMGWNKPILTDSGGFQVFSLGDLRKITEEGVTFASPINGDRLFLSPEVSMQIQKALNSDIVMQF
DECTPYATNGVPTTHREAAESMRMSLRWAKRSLDEFERLGNPNALFGIVQGGMYEDLRDESLAGLSALGFHGLAIGGLSV
GEPKEDMMRVLEHVAPRLPADKPHYLMGVGTPEDLVAGVAAGVDMFDCVMPTRNARNGWLFTRFGDVKIRNATHKNSLKP
LDETCGCYTCRNFSRGYLHHLHRVGEILGAQLNTIHNLHYYLDLMREIREAIETHTFEAFQKRFAQDRARGVD

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI13654276, Length=356, Percent_Identity=44.9438202247191, Blast_Score=299, Evalue=4e-81,
Organism=Escherichia coli, GI1786607, Length=374, Percent_Identity=66.5775401069519, Blast_Score=504, Evalue=1e-144,
Organism=Caenorhabditis elegans, GI17544680, Length=371, Percent_Identity=43.6657681940701, Blast_Score=306, Evalue=1e-83,
Organism=Caenorhabditis elegans, GI25146502, Length=138, Percent_Identity=28.9855072463768, Blast_Score=76, Evalue=3e-14,
Organism=Drosophila melanogaster, GI19920510, Length=381, Percent_Identity=44.3569553805774, Blast_Score=321, Evalue=4e-88,
Organism=Drosophila melanogaster, GI21355311, Length=158, Percent_Identity=29.1139240506329, Blast_Score=84, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004803
- InterPro:   IPR002616 [H]

Pfam domain/function: PF01702 TGT [H]

EC number: =2.4.2.29 [H]

Molecular weight: Translated: 43841; Mature: 43709

Theoretical pI: Translated: 6.99; Mature: 6.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTEGPSHDTSAGIRPHGGLKFELLTTDGRARRGRVTLNHGVVETPIFMPVGTYGTVKAV
CCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCEEEECCCEEECCEEEECCCCCCEECC
QPRELDEMRAQIILGNTFHLWLRPGLETIGAHGGLHRFMGWNKPILTDSGGFQVFSLGDL
CCCCHHHHHHEEEECCEEEEEECCCHHHHHCCCCHHHHCCCCCCEEECCCCEEEEECCHH
RKITEEGVTFASPINGDRLFLSPEVSMQIQKALNSDIVMQFDECTPYATNGVPTTHREAA
HHHHHCCCEEECCCCCCEEEECCCHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCHHHHH
ESMRMSLRWAKRSLDEFERLGNPNALFGIVQGGMYEDLRDESLAGLSALGFHGLAIGGLS
HHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHCHHHHCCCCEEECCCC
VGEPKEDMMRVLEHVAPRLPADKPHYLMGVGTPEDLVAGVAAGVDMFDCVMPTRNARNGW
CCCCHHHHHHHHHHHHHCCCCCCCCEEEECCCHHHHHHHHHHCCHHHHHHCCCCCCCCCE
LFTRFGDVKIRNATHKNSLKPLDETCGCYTCRNFSRGYLHHLHRVGEILGAQLNTIHNLH
EEEEECCEEEECCCCCCCCCCHHHHCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
YYLDLMREIREAIETHTFEAFQKRFAQDRARGVD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TTEGPSHDTSAGIRPHGGLKFELLTTDGRARRGRVTLNHGVVETPIFMPVGTYGTVKAV
CCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCEEEECCCEEECCEEEECCCCCCEECC
QPRELDEMRAQIILGNTFHLWLRPGLETIGAHGGLHRFMGWNKPILTDSGGFQVFSLGDL
CCCCHHHHHHEEEECCEEEEEECCCHHHHHCCCCHHHHCCCCCCEEECCCCEEEEECCHH
RKITEEGVTFASPINGDRLFLSPEVSMQIQKALNSDIVMQFDECTPYATNGVPTTHREAA
HHHHHCCCEEECCCCCCEEEECCCHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCHHHHH
ESMRMSLRWAKRSLDEFERLGNPNALFGIVQGGMYEDLRDESLAGLSALGFHGLAIGGLS
HHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHCHHHHCCCCEEECCCC
VGEPKEDMMRVLEHVAPRLPADKPHYLMGVGTPEDLVAGVAAGVDMFDCVMPTRNARNGW
CCCCHHHHHHHHHHHHHCCCCCCCCEEEECCCHHHHHHHHHHCCHHHHHHCCCCCCCCCE
LFTRFGDVKIRNATHKNSLKPLDETCGCYTCRNFSRGYLHHLHRVGEILGAQLNTIHNLH
EEEEECCEEEECCCCCCCCCCHHHHCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
YYLDLMREIREAIETHTFEAFQKRFAQDRARGVD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA