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The map label for this gene is miaA [H]

Identifier: 237813667

GI number: 237813667

Start: 3326372

End: 3327346

Strand: Direct

Name: miaA [H]

Synonym: GBP346_A3444

Alternate gene names: 237813667

Gene position: 3326372-3327346 (Clockwise)

Preceding gene: 237813666

Following gene: 237813670

Centisome position: 81.16

GC content: 73.54

Gene sequence:

>975_bases
ATGAGCGAACGCAACGCGGCGTCCGCGCGCACGGTCGCGTGCCTGCTCGGCCCCACCGCCTCCGGCAAGACAGCCGCCGC
GCTCGCGCTCGCCGCGCGCCGGCCGATCGAGATCGTCAGCGTCGATTCGGCGCTCGTCTACCGCGGGATGGACATCGGCA
CCGCGAAGCCGACACGCGACGAACGCGCGGCCGTGCCGCATCATCTGATCGACATCGTCGATCCGGCCGACGCGTACTCG
GCGGCCGAATTCCGCGCCGATGCATTACGGCTCGTGGCGCAAATCGCGGCGCGCGGCCGCACGCCGCTCCTCGCGGGCGG
CACGATGCTCTACTACAGGGCGCTGACGCAGGGGCTCAACGATCTGCCCGCGGCGGACCCCGACGTGCGCGCCACGCTCG
ACGCCGACGCCGCGCGCGACGGCTGGCCCGCGCTGCACGCGCGCCTCGCGGGCATCGATCCGGCGACGGCCGCGCGGCTC
GCGCCGAACGATTCGCAGCGGATCCAGCGCGCGCTCGAAGTGTATCTGCTGACCGGACAGCCGATGTCGGCGCTCCTCGC
CGCGCCGCCGCGAGACGACGACGCGGCGGCGGGCTTGCGGTTCGTGCCGGTCGCGCTCGAGCCGTCGGAGCGCGCCGTGC
TGCACGCGCGCATCGCCGCGCGCTTCGACGCGATGCTGGAGGCGGGTTTCATCGACGAGGTCGAGCGCCTGCGCCGGCGC
GACGATCTGCATCTCGGGCTGCCGTCGATGCGCTGCGTCGGCTACCGGCAGGCGTGGGAGTATCTCGACGGCTGCACCGA
CTACCGGACGATGCGCGACAAGGGCATCTTCGCGACCCGGCAGCTGTGCAAGCGCCAGTTGACGTGGCTGCGCGCGATGC
CGGAGCGGATCGTCGTCGACTGCTGCACGCCGGACGCGACCGTGCGCGCGGTCGACGCGCTCGAGCGGGTGCTCGACGGC
CGCGCGCCGGCTTGA

Upstream 100 bases:

>100_bases
AGATGGAGGCGACCGAGCGCGCGGATCAGTGCAACCATGGCCGGCCGACCTGGTATCAACTGACGCTCGGCGATCTCGAC
AAGCTTTTCATGCGCGGCCA

Downstream 100 bases:

>100_bases
CGCGCGACGCGTGTTCGTCAGGCGGCCGACGCGAGCAGGTTCGCGTCGCGCACGAGCCGCCAGCGGCCTTCCGACTTGCG
CAGGATCGTGAGCGTATGCG

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase [H]

Number of amino acids: Translated: 324; Mature: 323

Protein sequence:

>324_residues
MSERNAASARTVACLLGPTASGKTAAALALAARRPIEIVSVDSALVYRGMDIGTAKPTRDERAAVPHHLIDIVDPADAYS
AAEFRADALRLVAQIAARGRTPLLAGGTMLYYRALTQGLNDLPAADPDVRATLDADAARDGWPALHARLAGIDPATAARL
APNDSQRIQRALEVYLLTGQPMSALLAAPPRDDDAAAGLRFVPVALEPSERAVLHARIAARFDAMLEAGFIDEVERLRRR
DDLHLGLPSMRCVGYRQAWEYLDGCTDYRTMRDKGIFATRQLCKRQLTWLRAMPERIVVDCCTPDATVRAVDALERVLDG
RAPA

Sequences:

>Translated_324_residues
MSERNAASARTVACLLGPTASGKTAAALALAARRPIEIVSVDSALVYRGMDIGTAKPTRDERAAVPHHLIDIVDPADAYS
AAEFRADALRLVAQIAARGRTPLLAGGTMLYYRALTQGLNDLPAADPDVRATLDADAARDGWPALHARLAGIDPATAARL
APNDSQRIQRALEVYLLTGQPMSALLAAPPRDDDAAAGLRFVPVALEPSERAVLHARIAARFDAMLEAGFIDEVERLRRR
DDLHLGLPSMRCVGYRQAWEYLDGCTDYRTMRDKGIFATRQLCKRQLTWLRAMPERIVVDCCTPDATVRAVDALERVLDG
RAPA
>Mature_323_residues
SERNAASARTVACLLGPTASGKTAAALALAARRPIEIVSVDSALVYRGMDIGTAKPTRDERAAVPHHLIDIVDPADAYSA
AEFRADALRLVAQIAARGRTPLLAGGTMLYYRALTQGLNDLPAADPDVRATLDADAARDGWPALHARLAGIDPATAARLA
PNDSQRIQRALEVYLLTGQPMSALLAAPPRDDDAAAGLRFVPVALEPSERAVLHARIAARFDAMLEAGFIDEVERLRRRD
DLHLGLPSMRCVGYRQAWEYLDGCTDYRTMRDKGIFATRQLCKRQLTWLRAMPERIVVDCCTPDATVRAVDALERVLDGR
APA

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A) [H]

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family [H]

Homologues:

Organism=Homo sapiens, GI31581534, Length=308, Percent_Identity=30.8441558441558, Blast_Score=127, Evalue=1e-29,
Organism=Escherichia coli, GI1790613, Length=305, Percent_Identity=50.8196721311475, Blast_Score=300, Evalue=7e-83,
Organism=Caenorhabditis elegans, GI25144712, Length=276, Percent_Identity=27.8985507246377, Blast_Score=113, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6324848, Length=298, Percent_Identity=27.8523489932886, Blast_Score=130, Evalue=4e-31,
Organism=Drosophila melanogaster, GI24649830, Length=286, Percent_Identity=33.2167832167832, Blast_Score=132, Evalue=3e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002627
- InterPro:   IPR018022 [H]

Pfam domain/function: PF01715 IPPT [H]

EC number: =2.5.1.75 [H]

Molecular weight: Translated: 35127; Mature: 34995

Theoretical pI: Translated: 7.43; Mature: 7.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSERNAASARTVACLLGPTASGKTAAALALAARRPIEIVSVDSALVYRGMDIGTAKPTRD
CCCCCCCHHHEEEEEECCCCCCCHHHHHHHHHCCCEEEEEECHHHHHCCCCCCCCCCCCC
ERAAVPHHLIDIVDPADAYSAAEFRADALRLVAQIAARGRTPLLAGGTMLYYRALTQGLN
HHHCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHCHH
DLPAADPDVRATLDADAARDGWPALHARLAGIDPATAARLAPNDSQRIQRALEVYLLTGQ
CCCCCCCCCEEEECCCCCCCCCHHHHHHHCCCCHHHHHHCCCCHHHHHHHHHHEEEECCC
PMSALLAAPPRDDDAAAGLRFVPVALEPSERAVLHARIAARFDAMLEAGFIDEVERLRRR
CHHHHHCCCCCCCCHHCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHC
DDLHLGLPSMRCVGYRQAWEYLDGCTDYRTMRDKGIFATRQLCKRQLTWLRAMPERIVVD
CCCCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCHHEEEE
CCTPDATVRAVDALERVLDGRAPA
CCCCCHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
SERNAASARTVACLLGPTASGKTAAALALAARRPIEIVSVDSALVYRGMDIGTAKPTRD
CCCCCCHHHEEEEEECCCCCCCHHHHHHHHHCCCEEEEEECHHHHHCCCCCCCCCCCCC
ERAAVPHHLIDIVDPADAYSAAEFRADALRLVAQIAARGRTPLLAGGTMLYYRALTQGLN
HHHCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHCHH
DLPAADPDVRATLDADAARDGWPALHARLAGIDPATAARLAPNDSQRIQRALEVYLLTGQ
CCCCCCCCCEEEECCCCCCCCCHHHHHHHCCCCHHHHHHCCCCHHHHHHHHHHEEEECCC
PMSALLAAPPRDDDAAAGLRFVPVALEPSERAVLHARIAARFDAMLEAGFIDEVERLRRR
CHHHHHCCCCCCCCHHCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHC
DDLHLGLPSMRCVGYRQAWEYLDGCTDYRTMRDKGIFATRQLCKRQLTWLRAMPERIVVD
CCCCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCHHEEEE
CCTPDATVRAVDALERVLDGRAPA
CCCCCHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA