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The map label for this gene is trmB [H]

Identifier: 237813460

GI number: 237813460

Start: 3125920

End: 3126717

Strand: Direct

Name: trmB [H]

Synonym: GBP346_A3236

Alternate gene names: 237813460

Gene position: 3125920-3126717 (Clockwise)

Preceding gene: 237813457

Following gene: 237813465

Centisome position: 76.27

GC content: 70.3

Gene sequence:

>798_bases
ATGATCCACGACGACGACCCGAACGCCCCCGGCGCGCCGCACGACGACGACGCCACCGCCGCCCCCGCCTCGGCAACCCG
CGCCGCGCCGGCCGCCGGTGACGACGACGACGCGAACCCGCTCCATCTGCGCCGCATCCGCAGCTTCGTCACGCGCGCCG
GCCGCGTATCCACCGGCCAGCGCCGCGCAATCGACGAGCTCGGCCCGCGCTTCGTCATTCCGTACGGCAGCGCGCAGCCG
GACTGGGACGCCATCTTCGGCCGCCGCGCGCCGCGCGTGCTGGAAATCGGCTTCGGGATGGGCGCTTCGACGGCGGAAAT
CGCCGCGCTGCGCCCCGGCGACGACTTCATCGGCGTCGAGGTACACGAGCCGGGCGTCGGCGCGCTGCTGAAGCTGATCG
GCGAGCAGCAACTGTCGAACATCCGGATCATTCAGCACGACGCGGTCGAGGTGCTCGCGCAGATGATCGCGCCCGACAGC
CTCGACGGCGTGCACATCTTCTTCCCGGACCCGTGGCACAAGGCGCGCCATCACAAGCGCCGGCTGATCCAGCCGCCGTT
CGTCGCGCAGCTCGCGGCGCATCTCAAACCCGGCGCATACCTGCATTGCGCAACCGACTGGCAGAACTACGCGGAACAGA
TGCTCGAGGTGCTGAGCGCCGACCCGTCGCTCGAAAACACCGCGCAAGACTATGCGCCCCGCCCCGGCTATCGTCCGGTG
ACGAAGTTCGAGCGCCGCGGCCTGCGGCTCGGGCACGGCGTATGGGATCTCGTGTTCAGAAAGAAGCGCGCCGGCTGA

Upstream 100 bases:

>100_bases
TCGTATTTCATACGCAGTAAGGGCGGCCCGTCTCGAATGCGGCGAATGCGACGCCGTCGCGCACCGCGCCTGCGGCCCGC
CCCTCGCCTTCATTGTCACG

Downstream 100 bases:

>100_bases
CGCGCGACGCCGCGCGCATTACGCGCGGCGTCGGCCGGCATGCCCACCGGTCGAACCCGAAAGCCCGCAACGGCGGCGGC
AGCAGCGCGCTCACCGCCGC

Product: tRNA (guanine-N(7)-)-methyltransferase

Products: NA

Alternate protein names: tRNA(m7G46)-methyltransferase [H]

Number of amino acids: Translated: 265; Mature: 265

Protein sequence:

>265_residues
MIHDDDPNAPGAPHDDDATAAPASATRAAPAAGDDDDANPLHLRRIRSFVTRAGRVSTGQRRAIDELGPRFVIPYGSAQP
DWDAIFGRRAPRVLEIGFGMGASTAEIAALRPGDDFIGVEVHEPGVGALLKLIGEQQLSNIRIIQHDAVEVLAQMIAPDS
LDGVHIFFPDPWHKARHHKRRLIQPPFVAQLAAHLKPGAYLHCATDWQNYAEQMLEVLSADPSLENTAQDYAPRPGYRPV
TKFERRGLRLGHGVWDLVFRKKRAG

Sequences:

>Translated_265_residues
MIHDDDPNAPGAPHDDDATAAPASATRAAPAAGDDDDANPLHLRRIRSFVTRAGRVSTGQRRAIDELGPRFVIPYGSAQP
DWDAIFGRRAPRVLEIGFGMGASTAEIAALRPGDDFIGVEVHEPGVGALLKLIGEQQLSNIRIIQHDAVEVLAQMIAPDS
LDGVHIFFPDPWHKARHHKRRLIQPPFVAQLAAHLKPGAYLHCATDWQNYAEQMLEVLSADPSLENTAQDYAPRPGYRPV
TKFERRGLRLGHGVWDLVFRKKRAG
>Mature_265_residues
MIHDDDPNAPGAPHDDDATAAPASATRAAPAAGDDDDANPLHLRRIRSFVTRAGRVSTGQRRAIDELGPRFVIPYGSAQP
DWDAIFGRRAPRVLEIGFGMGASTAEIAALRPGDDFIGVEVHEPGVGALLKLIGEQQLSNIRIIQHDAVEVLAQMIAPDS
LDGVHIFFPDPWHKARHHKRRLIQPPFVAQLAAHLKPGAYLHCATDWQNYAEQMLEVLSADPSLENTAQDYAPRPGYRPV
TKFERRGLRLGHGVWDLVFRKKRAG

Specific function: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA [H]

COG id: COG0220

COG function: function code R; Predicted S-adenosylmethionine-dependent methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. TrmB family [H]

Homologues:

Organism=Escherichia coli, GI1789330, Length=229, Percent_Identity=53.7117903930131, Blast_Score=249, Evalue=2e-67,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003358 [H]

Pfam domain/function: PF02390 Methyltransf_4 [H]

EC number: =2.1.1.33 [H]

Molecular weight: Translated: 29121; Mature: 29121

Theoretical pI: Translated: 6.97; Mature: 6.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIHDDDPNAPGAPHDDDATAAPASATRAAPAAGDDDDANPLHLRRIRSFVTRAGRVSTGQ
CCCCCCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCH
RRAIDELGPRFVIPYGSAQPDWDAIFGRRAPRVLEIGFGMGASTAEIAALRPGDDFIGVE
HHHHHHCCCEEEEECCCCCCCHHHHHCCCCCEEEEECCCCCCCCHHEEEECCCCCEEEEE
VHEPGVGALLKLIGEQQLSNIRIIQHDAVEVLAQMIAPDSLDGVHIFFPDPWHKARHHKR
EECCCHHHHHHHHHHHHHCCEEEEHHHHHHHHHHHHCCCCCCCEEEECCCHHHHHHHHHH
RLIQPPFVAQLAAHLKPGAYLHCATDWQNYAEQMLEVLSADPSLENTAQDYAPRPGYRPV
HCCCCCHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCCCH
TKFERRGLRLGHGVWDLVFRKKRAG
HHHHHCCCCCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MIHDDDPNAPGAPHDDDATAAPASATRAAPAAGDDDDANPLHLRRIRSFVTRAGRVSTGQ
CCCCCCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCH
RRAIDELGPRFVIPYGSAQPDWDAIFGRRAPRVLEIGFGMGASTAEIAALRPGDDFIGVE
HHHHHHCCCEEEEECCCCCCCHHHHHCCCCCEEEEECCCCCCCCHHEEEECCCCCEEEEE
VHEPGVGALLKLIGEQQLSNIRIIQHDAVEVLAQMIAPDSLDGVHIFFPDPWHKARHHKR
EECCCHHHHHHHHHHHHHCCEEEEHHHHHHHHHHHHCCCCCCCEEEECCCHHHHHHHHHH
RLIQPPFVAQLAAHLKPGAYLHCATDWQNYAEQMLEVLSADPSLENTAQDYAPRPGYRPV
HCCCCCHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCCCH
TKFERRGLRLGHGVWDLVFRKKRAG
HHHHHCCCCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA