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The map label for this gene is aceF [H]

Identifier: 237813031

GI number: 237813031

Start: 2718286

End: 2719929

Strand: Reverse

Name: aceF [H]

Synonym: GBP346_A2791

Alternate gene names: 237813031

Gene position: 2719929-2718286 (Counterclockwise)

Preceding gene: 237813032

Following gene: 237813030

Centisome position: 66.36

GC content: 69.34

Gene sequence:

>1644_bases
ATGAGTCAAGCGATCGAAGTCAAGGTGCCGGATATCGGCGATTACAAGGACGTGCCCGTCATCGAAGTGCTCGTGAAGCC
GGGCGATGCGGTCGAGCCCGAGCAGTCGCTCGTCACGCTCGAATCGGACAAGGCGACGATGGATGTGCCGAGCCCGTCGG
CGGGCACGGTCAAGGAAGTGAAGGTGAAGGTCGGCGACGCGGTGTCGCAAGGCTCGCTGATCGTGCTGCTCGACGGCGCG
CAGGCGGCGGCCCAGCCCGCGCAGGCGAACGGCGCCGCGACGAGCGCCGCGCAGCCGGCGGCGGCGCCCGCTGCCGCGCC
TGCGCCGGCGGCGGCCGCGGGCGGCGGCACGGTCGACGTGAAGGTGCCGGACATCGGCGACTACAAGGACGTGCCCGTCA
TCGAGATCGCCGTGAAGATCGGCGACACGGTCGAGAAGGAGCAGTCGCTCGTCACGCTCGAATCGGACAAGGCGACGATG
GACGTGCCGAGCCCGGCCGCGGGCGTCGTCAAGGACATCAAGGTGAAGGTCGGCGATGCGGTGTCGGAAGGCTCGCTGAT
CGTCGTGCTCGAAGCGTCGGGCGGCGCCGCCGCGAGCGCGCCGCAGGCGGCCGCGCCCGCCCCCGCGCCGGCCGCGCCGG
CTCCGGCGCCCGCGCCGCAGGCCGCACCCGCGGCTGCGCCGGCCCCCGCGCAGGCACCGGCACCCGCCGCGAGCGGCGAG
TACCGCGCGAGCCACGCGTCGCCGTCGGTGCGCAAGTTCGCGCGCGAGCTCGGCGTCGACGTGTCGCGCGTCACGGGCAC
GGGGCCGAAGAGCCGTATCACGAAGGACGACGTCACCGCGTTCGTGAAGGGGGTGATGACGGGGCAGCGCGCGGCGCCCG
GCGCCGCGGCCGCGCCCGCGGGCGGCGGCGAGCTGAACCTGCTGCCGTGGCCGAAGGTCGACTTCTCGAAGTTCGGCCCG
TTCGAGGCGAAGCCGCTGTCGCGCATCAAGAAGATCTCGGGCGCGAACCTGCATCGCAACTGGGTGATGATCCCGCACGT
CACGAACAACGACGAGGCGGACATCACCGAGCTTGAAGCGCTGCGCGTGCAACTGAACAAGGAGCACGAGAAGGCGGGCG
TGAAGTTCACGATGCTCGCGTTCGTGATCAAGGCGGTCGTCGCCGCGCTGAAGAAGTTCCCGACCTTCAACGCGAGCCTC
GACGGCGACAACCTCGTGTTCAAGCAGTACTACCACATCGGTTTCGCCGCCGACACGCCGAACGGCCTCGTCGTGCCGGT
GATCCGCGACGCGGACAAGAAGGGGCTCGTCGACATCGCGAAGGAAATGGCCGAGCTGTCGAAGGCCGCGCGCGAAGGCA
AGCTCAAGCCGGACCAGATGCAGGGCGGCTGCTTCTCGATCTCGTCGCTTGGCGGGATCGGCGGCACGCACTTCACGCCG
ATCATCAATGCGCCGGAAGTGGCGATCCTCGGGCTGTCGCGCGGCCAGATGAAGCCGGTGTGGGACGGCAAGCAGTTTGT
GCCGCGCCTCATGCTGCCGCTGTCGCTGTCGTATGACCATCGCGTGATCGATGGCGCGGAAGCCGCGCGGTTCAATGCGT
ATCTCGGCGCGTTGCTTGCCGATTTCCGTCGCATCATTCTTTGA

Upstream 100 bases:

>100_bases
CGCTGTCGACGTTTTCGACGAACGAAGCGGAGGCGGGTGGCGCGGCTGCGCCTTGCATGGGATCGGAGCGGCCGCTCCGA
TCGACAGGAGACTGAAACAG

Downstream 100 bases:

>100_bases
TGAGCGTGGGAGTGGCTCGCGGGGGCGTGGGTTTTTTGTCGGTGATCCGCGCTTCGTCGTTGAACCTGCTGTGCTAACGG
TCCATTAGCGTCGCCCCTGC

Product: dihydrolipoamide acetyltransferase

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2 [H]

Number of amino acids: Translated: 547; Mature: 546

Protein sequence:

>547_residues
MSQAIEVKVPDIGDYKDVPVIEVLVKPGDAVEPEQSLVTLESDKATMDVPSPSAGTVKEVKVKVGDAVSQGSLIVLLDGA
QAAAQPAQANGAATSAAQPAAAPAAAPAPAAAAGGGTVDVKVPDIGDYKDVPVIEIAVKIGDTVEKEQSLVTLESDKATM
DVPSPAAGVVKDIKVKVGDAVSEGSLIVVLEASGGAAASAPQAAAPAPAPAAPAPAPAPQAAPAAAPAPAQAPAPAASGE
YRASHASPSVRKFARELGVDVSRVTGTGPKSRITKDDVTAFVKGVMTGQRAAPGAAAAPAGGGELNLLPWPKVDFSKFGP
FEAKPLSRIKKISGANLHRNWVMIPHVTNNDEADITELEALRVQLNKEHEKAGVKFTMLAFVIKAVVAALKKFPTFNASL
DGDNLVFKQYYHIGFAADTPNGLVVPVIRDADKKGLVDIAKEMAELSKAAREGKLKPDQMQGGCFSISSLGGIGGTHFTP
IINAPEVAILGLSRGQMKPVWDGKQFVPRLMLPLSLSYDHRVIDGAEAARFNAYLGALLADFRRIIL

Sequences:

>Translated_547_residues
MSQAIEVKVPDIGDYKDVPVIEVLVKPGDAVEPEQSLVTLESDKATMDVPSPSAGTVKEVKVKVGDAVSQGSLIVLLDGA
QAAAQPAQANGAATSAAQPAAAPAAAPAPAAAAGGGTVDVKVPDIGDYKDVPVIEIAVKIGDTVEKEQSLVTLESDKATM
DVPSPAAGVVKDIKVKVGDAVSEGSLIVVLEASGGAAASAPQAAAPAPAPAAPAPAPAPQAAPAAAPAPAQAPAPAASGE
YRASHASPSVRKFARELGVDVSRVTGTGPKSRITKDDVTAFVKGVMTGQRAAPGAAAAPAGGGELNLLPWPKVDFSKFGP
FEAKPLSRIKKISGANLHRNWVMIPHVTNNDEADITELEALRVQLNKEHEKAGVKFTMLAFVIKAVVAALKKFPTFNASL
DGDNLVFKQYYHIGFAADTPNGLVVPVIRDADKKGLVDIAKEMAELSKAAREGKLKPDQMQGGCFSISSLGGIGGTHFTP
IINAPEVAILGLSRGQMKPVWDGKQFVPRLMLPLSLSYDHRVIDGAEAARFNAYLGALLADFRRIIL
>Mature_546_residues
SQAIEVKVPDIGDYKDVPVIEVLVKPGDAVEPEQSLVTLESDKATMDVPSPSAGTVKEVKVKVGDAVSQGSLIVLLDGAQ
AAAQPAQANGAATSAAQPAAAPAAAPAPAAAAGGGTVDVKVPDIGDYKDVPVIEIAVKIGDTVEKEQSLVTLESDKATMD
VPSPAAGVVKDIKVKVGDAVSEGSLIVVLEASGGAAASAPQAAAPAPAPAAPAPAPAPQAAPAAAPAPAQAPAPAASGEY
RASHASPSVRKFARELGVDVSRVTGTGPKSRITKDDVTAFVKGVMTGQRAAPGAAAAPAGGGELNLLPWPKVDFSKFGPF
EAKPLSRIKKISGANLHRNWVMIPHVTNNDEADITELEALRVQLNKEHEKAGVKFTMLAFVIKAVVAALKKFPTFNASLD
GDNLVFKQYYHIGFAADTPNGLVVPVIRDADKKGLVDIAKEMAELSKAAREGKLKPDQMQGGCFSISSLGGIGGTHFTPI
INAPEVAILGLSRGQMKPVWDGKQFVPRLMLPLSLSYDHRVIDGAEAARFNAYLGALLADFRRIIL

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 lipoyl-binding domains [H]

Homologues:

Organism=Homo sapiens, GI110671329, Length=460, Percent_Identity=29.3478260869565, Blast_Score=171, Evalue=2e-42,
Organism=Homo sapiens, GI31711992, Length=424, Percent_Identity=31.8396226415094, Blast_Score=159, Evalue=9e-39,
Organism=Homo sapiens, GI203098816, Length=487, Percent_Identity=27.7207392197125, Blast_Score=140, Evalue=3e-33,
Organism=Homo sapiens, GI203098753, Length=443, Percent_Identity=28.4424379232506, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI19923748, Length=227, Percent_Identity=34.8017621145374, Blast_Score=125, Evalue=1e-28,
Organism=Homo sapiens, GI260898739, Length=153, Percent_Identity=37.2549019607843, Blast_Score=98, Evalue=2e-20,
Organism=Escherichia coli, GI1786305, Length=546, Percent_Identity=52.5641025641026, Blast_Score=514, Evalue=1e-147,
Organism=Escherichia coli, GI1786946, Length=433, Percent_Identity=31.8706697459584, Blast_Score=190, Evalue=2e-49,
Organism=Caenorhabditis elegans, GI17537937, Length=424, Percent_Identity=28.7735849056604, Blast_Score=172, Evalue=5e-43,
Organism=Caenorhabditis elegans, GI17560088, Length=442, Percent_Identity=31.2217194570136, Blast_Score=150, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI25146366, Length=423, Percent_Identity=30.9692671394799, Blast_Score=150, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI17538894, Length=309, Percent_Identity=33.3333333333333, Blast_Score=121, Evalue=8e-28,
Organism=Saccharomyces cerevisiae, GI6320352, Length=415, Percent_Identity=30.1204819277108, Blast_Score=177, Evalue=4e-45,
Organism=Saccharomyces cerevisiae, GI6324258, Length=431, Percent_Identity=28.3062645011601, Blast_Score=130, Evalue=4e-31,
Organism=Drosophila melanogaster, GI18859875, Length=431, Percent_Identity=32.9466357308585, Blast_Score=184, Evalue=1e-46,
Organism=Drosophila melanogaster, GI24582497, Length=232, Percent_Identity=31.8965517241379, Blast_Score=127, Evalue=3e-29,
Organism=Drosophila melanogaster, GI20129315, Length=232, Percent_Identity=31.8965517241379, Blast_Score=126, Evalue=3e-29,
Organism=Drosophila melanogaster, GI24645909, Length=207, Percent_Identity=35.7487922705314, Blast_Score=123, Evalue=4e-28,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 912 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR006256
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 56367; Mature: 56236

Theoretical pI: Translated: 6.43; Mature: 6.43

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQAIEVKVPDIGDYKDVPVIEVLVKPGDAVEPEQSLVTLESDKATMDVPSPSAGTVKEV
CCCEEEEECCCCCCCCCCCEEEEEECCCCCCCCCCCEEEEECCCCEEECCCCCCCCCEEE
KVKVGDAVSQGSLIVLLDGAQAAAQPAQANGAATSAAQPAAAPAAAPAPAAAAGGGTVDV
EEEECCCCCCCCEEEEECCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEE
KVPDIGDYKDVPVIEIAVKIGDTVEKEQSLVTLESDKATMDVPSPAAGVVKDIKVKVGDA
ECCCCCCCCCCCEEEEEEECCCCHHCCCCEEEEECCCCEECCCCCHHHHHHHEEEEECCC
VSEGSLIVVLEASGGAAASAPQAAAPAPAPAAPAPAPAPQAAPAAAPAPAQAPAPAASGE
CCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
YRASHASPSVRKFARELGVDVSRVTGTGPKSRITKDDVTAFVKGVMTGQRAAPGAAAAPA
CCCCCCCHHHHHHHHHHCCEEEEECCCCCHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCC
GGGELNLLPWPKVDFSKFGPFEAKPLSRIKKISGANLHRNWVMIPHVTNNDEADITELEA
CCCEEEECCCCCCCHHHCCCCCCCHHHHHHHHCCCCEECCEEEEEEECCCCCCCHHHHHH
LRVQLNKEHEKAGVKFTMLAFVIKAVVAALKKFPTFNASLDGDNLVFKQYYHIGFAADTP
HHHHHCCHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEEEEEECCC
NGLVVPVIRDADKKGLVDIAKEMAELSKAAREGKLKPDQMQGGCFSISSLGGIGGTHFTP
CCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHCCCEEEECCCCCCCCCCCCE
IINAPEVAILGLSRGQMKPVWDGKQFVPRLMLPLSLSYDHRVIDGAEAARFNAYLGALLA
ECCCCCEEEEECCCCCCCCCCCCHHHHHHHHCCEECCCCCEEECCCHHHHHHHHHHHHHH
DFRRIIL
HHHHHHC
>Mature Secondary Structure 
SQAIEVKVPDIGDYKDVPVIEVLVKPGDAVEPEQSLVTLESDKATMDVPSPSAGTVKEV
CCEEEEECCCCCCCCCCCEEEEEECCCCCCCCCCCEEEEECCCCEEECCCCCCCCCEEE
KVKVGDAVSQGSLIVLLDGAQAAAQPAQANGAATSAAQPAAAPAAAPAPAAAAGGGTVDV
EEEECCCCCCCCEEEEECCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEE
KVPDIGDYKDVPVIEIAVKIGDTVEKEQSLVTLESDKATMDVPSPAAGVVKDIKVKVGDA
ECCCCCCCCCCCEEEEEEECCCCHHCCCCEEEEECCCCEECCCCCHHHHHHHEEEEECCC
VSEGSLIVVLEASGGAAASAPQAAAPAPAPAAPAPAPAPQAAPAAAPAPAQAPAPAASGE
CCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
YRASHASPSVRKFARELGVDVSRVTGTGPKSRITKDDVTAFVKGVMTGQRAAPGAAAAPA
CCCCCCCHHHHHHHHHHCCEEEEECCCCCHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCC
GGGELNLLPWPKVDFSKFGPFEAKPLSRIKKISGANLHRNWVMIPHVTNNDEADITELEA
CCCEEEECCCCCCCHHHCCCCCCCHHHHHHHHCCCCEECCEEEEEEECCCCCCCHHHHHH
LRVQLNKEHEKAGVKFTMLAFVIKAVVAALKKFPTFNASLDGDNLVFKQYYHIGFAADTP
HHHHHCCHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEEEEEECCC
NGLVVPVIRDADKKGLVDIAKEMAELSKAAREGKLKPDQMQGGCFSISSLGGIGGTHFTP
CCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHCCCEEEECCCCCCCCCCCCE
IINAPEVAILGLSRGQMKPVWDGKQFVPRLMLPLSLSYDHRVIDGAEAARFNAYLGALLA
ECCCCCEEEEECCCCCCCCCCCCHHHHHHHHCCEECCCCCEEECCCHHHHHHHHHHHHHH
DFRRIIL
HHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8021225 [H]