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The map label for this gene is lpdA_2 [H]

Identifier: 237813030

GI number: 237813030

Start: 2716204

End: 2717979

Strand: Reverse

Name: lpdA_2 [H]

Synonym: GBP346_A2790

Alternate gene names: 237813030

Gene position: 2717979-2716204 (Counterclockwise)

Preceding gene: 237813031

Following gene: 237813027

Centisome position: 66.32

GC content: 65.82

Gene sequence:

>1776_bases
ATGAGTCTCATCGAAGTCAAGGTTCCGGATATCGGCGATTTCAGCGGCGTCGATGTCATTGAGGTCAACGTCAAACCCGG
CGACGTGATCGAAAAAGAGCAAACGCTCATCACGCTCGAATCCGATAAAGCCTCCATGGAAGTGCCCAGCGACGTCGCCG
GCACCGTGAAGGAAATCAAGATCAAGGCCGGCGACAAAGTCTCGCAAGGCACCGTCATCGCCATCGTCGAAGCATCGGCA
GGCGCCGCCGCACCCGCACCCGCACCCGCGAAAGCCCCCGAAGCACCGAAGCCCGCAGCAGCTGCACCGGCTCCGGCCGC
CGCCCCGGCACCCGCGCCGCAAGCCGGCAGCTACAGCGGCTCCGCCGACATCGAGTGCGACATGCTCGTGCTCGGCGCCG
GCCCCGGCGGCTACTCGGCCGCGTTCCGCGCCGCCGACCTCGGCATGAAGACGGTGCTGGTCGAACGCTACTCGACGCTC
GGCGGCGTGTGCCTGAACGTCGGCTGCATTCCGTCAAAGGCATTGCTGCACACGGCGCTCGTCGTCGAGGAAGCGCAGGC
GCTCGCGTCGCACGGCATCACGTTCGGCAAGCCGCAGGTCGAGCTCGACAAGCTGCGCGATTTCAAGGGCGGCGTCGTCA
AGAAGCTGACGACGGGCCTCGCCGGCATGGCGAAGGCGCGCAAGGTCGAAGTGGTCACGGGCGTCGGCGCGTTCGTCGAT
CCGTATCACATGGAAGTGCAGAGCGAAAACGGCAAGAAGATCGTCAAGTTCAAGCAGGCGATCATCGCCGCCGGCTCGCA
GGCGGTGAAGCTGCCGTTCATGCCGGAAGACCCGCGCGTCGTCGATTCGACGGGCGCGCTCGAACTGCGTCAGTTGCCCA
AGCGCATGCTCGTGATCGGCGGCGGCATCATCGGCCTCGAAATGGCGACGGTGTACTCCACGCTCGGCGCCGAGATCGAC
GTCGTCGAAATGATGGACGGCCTGATGATGGGCGCGGACCGCGATCTCGTGAAGGTCTGGGAAAAGTACAACGCGAAGCG
CTTCGGCAACGTGATGCTCAAGACCAAGACGGTCGGCGCGCAAGCGAAGGAAGACGGCATCTACGTGACGTTCGAGGGCG
AGAAGGCGCCGGCGCAAGCGCAGCGCTACGACCTCGTGCTCGTCGCGGTGGGCCGCAGCCCGAACGGCAAGAAGATCGGC
GCCGACAAGGCGGGCGTGGCGGTCACCGACCGCGGCTTCATCGACGTCGACAAGCAGATGCGCACGAACGTGCCGCACAT
CTTCGCGATCGGCGACATCGTCGGCCAGCCGATGCTCGCGCACAAGGCCGTGCATGAAGGCCACGTCGCGGCCGAAGCCG
CGCACGGCGAGAAGGCGTACTTCGACGCGCTGCAGATCCCGTCGGTGGCGTACACCGATCCGGAAGTGGCATGGGCCGGC
AAGACGGAAGACCAGTGCAAGGCCGAAGGCATCAAGTACGGCAAGGCGGTGTTCCCGTGGGCCGCATCGGGCCGCGCGAT
CGCGAACGGCCGCGACGAGGGCTTCACGAAGCTGCTCTTCGACGAGGAAACCCACCGCGTGATCGGCGGCGGCATCGTCG
GCCTGAACGCGGGCGACCTGATCAGCGAAGTGTGCCTCGCGGTCGAGATGGGCGCGGACGCGGAAGACATCGGCAAGACG
ATCCATCCGCACCCGACGCTCGGCGAATCGATCGGCATGGCCGCCGAGCTGTACGAAGGCGTTTGCACGGATCTGCCGCC
GCAGCGCAAGAAGTAA

Upstream 100 bases:

>100_bases
GGTACTGCCCAGGTTCGCGTTCTTTGGTCAGGCCGTAGTCGCGGTTTTTCTCTGCGGGCTTCTCCCCCTCTGTCTGCAGC
CAATCAAGAAGGGGACAGTA

Downstream 100 bases:

>100_bases
GCGGCGCCGCCGTCCGGCCACGCTGCGACGGCGCAAGAACGGCGCTTCCCGAATCGGGCCGCACGCCGACAGCCGGCAGC
CGACAGCCGACAGCCGGCAG

Product: dihydrolipoyl dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of pyruvate and 2-oxoglutarate dehydrogenases complexes; Glycine cleavage system L protein [H]

Number of amino acids: Translated: 591; Mature: 590

Protein sequence:

>591_residues
MSLIEVKVPDIGDFSGVDVIEVNVKPGDVIEKEQTLITLESDKASMEVPSDVAGTVKEIKIKAGDKVSQGTVIAIVEASA
GAAAPAPAPAKAPEAPKPAAAAPAPAAAPAPAPQAGSYSGSADIECDMLVLGAGPGGYSAAFRAADLGMKTVLVERYSTL
GGVCLNVGCIPSKALLHTALVVEEAQALASHGITFGKPQVELDKLRDFKGGVVKKLTTGLAGMAKARKVEVVTGVGAFVD
PYHMEVQSENGKKIVKFKQAIIAAGSQAVKLPFMPEDPRVVDSTGALELRQLPKRMLVIGGGIIGLEMATVYSTLGAEID
VVEMMDGLMMGADRDLVKVWEKYNAKRFGNVMLKTKTVGAQAKEDGIYVTFEGEKAPAQAQRYDLVLVAVGRSPNGKKIG
ADKAGVAVTDRGFIDVDKQMRTNVPHIFAIGDIVGQPMLAHKAVHEGHVAAEAAHGEKAYFDALQIPSVAYTDPEVAWAG
KTEDQCKAEGIKYGKAVFPWAASGRAIANGRDEGFTKLLFDEETHRVIGGGIVGLNAGDLISEVCLAVEMGADAEDIGKT
IHPHPTLGESIGMAAELYEGVCTDLPPQRKK

Sequences:

>Translated_591_residues
MSLIEVKVPDIGDFSGVDVIEVNVKPGDVIEKEQTLITLESDKASMEVPSDVAGTVKEIKIKAGDKVSQGTVIAIVEASA
GAAAPAPAPAKAPEAPKPAAAAPAPAAAPAPAPQAGSYSGSADIECDMLVLGAGPGGYSAAFRAADLGMKTVLVERYSTL
GGVCLNVGCIPSKALLHTALVVEEAQALASHGITFGKPQVELDKLRDFKGGVVKKLTTGLAGMAKARKVEVVTGVGAFVD
PYHMEVQSENGKKIVKFKQAIIAAGSQAVKLPFMPEDPRVVDSTGALELRQLPKRMLVIGGGIIGLEMATVYSTLGAEID
VVEMMDGLMMGADRDLVKVWEKYNAKRFGNVMLKTKTVGAQAKEDGIYVTFEGEKAPAQAQRYDLVLVAVGRSPNGKKIG
ADKAGVAVTDRGFIDVDKQMRTNVPHIFAIGDIVGQPMLAHKAVHEGHVAAEAAHGEKAYFDALQIPSVAYTDPEVAWAG
KTEDQCKAEGIKYGKAVFPWAASGRAIANGRDEGFTKLLFDEETHRVIGGGIVGLNAGDLISEVCLAVEMGADAEDIGKT
IHPHPTLGESIGMAAELYEGVCTDLPPQRKK
>Mature_590_residues
SLIEVKVPDIGDFSGVDVIEVNVKPGDVIEKEQTLITLESDKASMEVPSDVAGTVKEIKIKAGDKVSQGTVIAIVEASAG
AAAPAPAPAKAPEAPKPAAAAPAPAAAPAPAPQAGSYSGSADIECDMLVLGAGPGGYSAAFRAADLGMKTVLVERYSTLG
GVCLNVGCIPSKALLHTALVVEEAQALASHGITFGKPQVELDKLRDFKGGVVKKLTTGLAGMAKARKVEVVTGVGAFVDP
YHMEVQSENGKKIVKFKQAIIAAGSQAVKLPFMPEDPRVVDSTGALELRQLPKRMLVIGGGIIGLEMATVYSTLGAEIDV
VEMMDGLMMGADRDLVKVWEKYNAKRFGNVMLKTKTVGAQAKEDGIYVTFEGEKAPAQAQRYDLVLVAVGRSPNGKKIGA
DKAGVAVTDRGFIDVDKQMRTNVPHIFAIGDIVGQPMLAHKAVHEGHVAAEAAHGEKAYFDALQIPSVAYTDPEVAWAGK
TEDQCKAEGIKYGKAVFPWAASGRAIANGRDEGFTKLLFDEETHRVIGGGIVGLNAGDLISEVCLAVEMGADAEDIGKTI
HPHPTLGESIGMAAELYEGVCTDLPPQRKK

Specific function: Lipoamide dehydrogenase is a component of the glycine cleavage system as well as of the alpha-ketoacid dehydrogenase complexes [H]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=455, Percent_Identity=41.3186813186813, Blast_Score=335, Evalue=9e-92,
Organism=Homo sapiens, GI50301238, Length=466, Percent_Identity=28.1115879828326, Blast_Score=150, Evalue=2e-36,
Organism=Homo sapiens, GI291045266, Length=432, Percent_Identity=26.1574074074074, Blast_Score=128, Evalue=1e-29,
Organism=Homo sapiens, GI148277071, Length=447, Percent_Identity=24.8322147651007, Blast_Score=119, Evalue=1e-26,
Organism=Homo sapiens, GI33519430, Length=447, Percent_Identity=24.8322147651007, Blast_Score=119, Evalue=1e-26,
Organism=Homo sapiens, GI33519428, Length=447, Percent_Identity=24.8322147651007, Blast_Score=119, Evalue=1e-26,
Organism=Homo sapiens, GI33519426, Length=447, Percent_Identity=24.8322147651007, Blast_Score=119, Evalue=1e-26,
Organism=Homo sapiens, GI148277065, Length=447, Percent_Identity=24.8322147651007, Blast_Score=118, Evalue=1e-26,
Organism=Homo sapiens, GI22035672, Length=455, Percent_Identity=26.8131868131868, Blast_Score=118, Evalue=2e-26,
Organism=Homo sapiens, GI291045268, Length=425, Percent_Identity=24.9411764705882, Blast_Score=110, Evalue=4e-24,
Organism=Escherichia coli, GI1786307, Length=473, Percent_Identity=65.53911205074, Blast_Score=632, Evalue=0.0,
Organism=Escherichia coli, GI87082354, Length=466, Percent_Identity=28.9699570815451, Blast_Score=174, Evalue=2e-44,
Organism=Escherichia coli, GI87081717, Length=452, Percent_Identity=25.4424778761062, Blast_Score=153, Evalue=3e-38,
Organism=Escherichia coli, GI1789915, Length=443, Percent_Identity=28.2167042889391, Blast_Score=144, Evalue=1e-35,
Organism=Escherichia coli, GI1786305, Length=73, Percent_Identity=60.2739726027397, Blast_Score=80, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI32565766, Length=462, Percent_Identity=42.8571428571429, Blast_Score=348, Evalue=3e-96,
Organism=Caenorhabditis elegans, GI17557007, Length=481, Percent_Identity=27.027027027027, Blast_Score=127, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI71982272, Length=442, Percent_Identity=25.1131221719457, Blast_Score=111, Evalue=9e-25,
Organism=Caenorhabditis elegans, GI71983429, Length=462, Percent_Identity=26.1904761904762, Blast_Score=110, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI71983419, Length=462, Percent_Identity=26.1904761904762, Blast_Score=110, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6321091, Length=458, Percent_Identity=40.3930131004367, Blast_Score=305, Evalue=9e-84,
Organism=Saccharomyces cerevisiae, GI6325240, Length=470, Percent_Identity=28.0851063829787, Blast_Score=191, Evalue=2e-49,
Organism=Saccharomyces cerevisiae, GI6325166, Length=467, Percent_Identity=26.7665952890792, Blast_Score=135, Evalue=3e-32,
Organism=Drosophila melanogaster, GI21358499, Length=471, Percent_Identity=43.0997876857749, Blast_Score=356, Evalue=3e-98,
Organism=Drosophila melanogaster, GI24640551, Length=517, Percent_Identity=27.2727272727273, Blast_Score=129, Evalue=4e-30,
Organism=Drosophila melanogaster, GI24640553, Length=484, Percent_Identity=27.2727272727273, Blast_Score=123, Evalue=3e-28,
Organism=Drosophila melanogaster, GI24640549, Length=482, Percent_Identity=27.3858921161826, Blast_Score=123, Evalue=5e-28,
Organism=Drosophila melanogaster, GI17737741, Length=472, Percent_Identity=24.364406779661, Blast_Score=103, Evalue=3e-22,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 61835; Mature: 61704

Theoretical pI: Translated: 5.43; Mature: 5.43

Prosite motif: PS00076 PYRIDINE_REDOX_1 ; PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLIEVKVPDIGDFSGVDVIEVNVKPGDVIEKEQTLITLESDKASMEVPSDVAGTVKEIK
CCEEEEECCCCCCCCCCEEEEEECCCCCCCCCCCEEEEEECCCCCCCCCCHHHCCEEEEE
IKAGDKVSQGTVIAIVEASAGAAAPAPAPAKAPEAPKPAAAAPAPAAAPAPAPQAGSYSG
EECCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
SADIECDMLVLGAGPGGYSAAFRAADLGMKTVLVERYSTLGGVCLNVGCIPSKALLHTAL
CCCCEEEEEEEECCCCCHHHHHHHHHCCHHHHHHHHHHHHCCEEEEECCCCCHHHHHHHH
VVEEAQALASHGITFGKPQVELDKLRDFKGGVVKKLTTGLAGMAKARKVEVVTGVGAFVD
HHHHHHHHHHCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHCCEEEEEECCCCCCC
PYHMEVQSENGKKIVKFKQAIIAAGSQAVKLPFMPEDPRVVDSTGALELRQLPKRMLVIG
CEEEEEECCCCCCHHHHHHHHHHCCCCEEECCCCCCCCCEECCCCCHHHHHCCCEEEEEC
GGIIGLEMATVYSTLGAEIDVVEMMDGLMMGADRDLVKVWEKYNAKRFGNVMLKTKTVGA
CCCHHHHHHHHHHHHCCCEEHHHHHHHHHCCCCHHHHHHHHHHCHHHHCCEEEEEEECCC
QAKEDGIYVTFEGEKAPAQAQRYDLVLVAVGRSPNGKKIGADKAGVAVTDRGFIDVDKQM
CCCCCCEEEEEECCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCCEEEECCCCEEECHHH
RTNVPHIFAIGDIVGQPMLAHKAVHEGHVAAEAAHGEKAYFDALQIPSVAYTDPEVAWAG
HCCCCEEEEEHHHHCCCHHHHHHHHCCCCEEECCCCCHHHHHHHCCCCEEECCCCEEECC
KTEDQCKAEGIKYGKAVFPWAASGRAIANGRDEGFTKLLFDEETHRVIGGGIVGLNAGDL
CCHHHHHHCCCCCCCEECCCCCCCCEEECCCCCCCEEEEECCCCCEEECCCEEECCHHHH
ISEVCLAVEMGADAEDIGKTIHPHPTLGESIGMAAELYEGVCTDLPPQRKK
HHHHHHHHHCCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
SLIEVKVPDIGDFSGVDVIEVNVKPGDVIEKEQTLITLESDKASMEVPSDVAGTVKEIK
CEEEEECCCCCCCCCCEEEEEECCCCCCCCCCCEEEEEECCCCCCCCCCHHHCCEEEEE
IKAGDKVSQGTVIAIVEASAGAAAPAPAPAKAPEAPKPAAAAPAPAAAPAPAPQAGSYSG
EECCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
SADIECDMLVLGAGPGGYSAAFRAADLGMKTVLVERYSTLGGVCLNVGCIPSKALLHTAL
CCCCEEEEEEEECCCCCHHHHHHHHHCCHHHHHHHHHHHHCCEEEEECCCCCHHHHHHHH
VVEEAQALASHGITFGKPQVELDKLRDFKGGVVKKLTTGLAGMAKARKVEVVTGVGAFVD
HHHHHHHHHHCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHCCEEEEEECCCCCCC
PYHMEVQSENGKKIVKFKQAIIAAGSQAVKLPFMPEDPRVVDSTGALELRQLPKRMLVIG
CEEEEEECCCCCCHHHHHHHHHHCCCCEEECCCCCCCCCEECCCCCHHHHHCCCEEEEEC
GGIIGLEMATVYSTLGAEIDVVEMMDGLMMGADRDLVKVWEKYNAKRFGNVMLKTKTVGA
CCCHHHHHHHHHHHHCCCEEHHHHHHHHHCCCCHHHHHHHHHHCHHHHCCEEEEEEECCC
QAKEDGIYVTFEGEKAPAQAQRYDLVLVAVGRSPNGKKIGADKAGVAVTDRGFIDVDKQM
CCCCCCEEEEEECCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCCEEEECCCCEEECHHH
RTNVPHIFAIGDIVGQPMLAHKAVHEGHVAAEAAHGEKAYFDALQIPSVAYTDPEVAWAG
HCCCCEEEEEHHHHCCCHHHHHHHHCCCCEEECCCCCHHHHHHHCCCCEEECCCCEEECC
KTEDQCKAEGIKYGKAVFPWAASGRAIANGRDEGFTKLLFDEETHRVIGGGIVGLNAGDL
CCHHHHHHCCCCCCCEECCCCCCCCEEECCCCCCCEEEEECCCCCEEECCCEEECCHHHH
ISEVCLAVEMGADAEDIGKTIHPHPTLGESIGMAAELYEGVCTDLPPQRKK
HHHHHHHHHCCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]