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The map label for this gene is fabI [H]

Identifier: 237812858

GI number: 237812858

Start: 2551765

End: 2552556

Strand: Direct

Name: fabI [H]

Synonym: GBP346_A2617

Alternate gene names: 237812858

Gene position: 2551765-2552556 (Clockwise)

Preceding gene: 237812851

Following gene: 237812860

Centisome position: 62.26

GC content: 66.04

Gene sequence:

>792_bases
ATGGGCTTTCTCGACGGTAAACGTATTCTGCTGACGGGCCTCTTGTCGAACCGTTCGATCGCTTACGGCATCGCCAAGGC
GTGCAAGCGCGAAGGCGCCGAGCTGGCGTTCACCTACGTCGGCGATCGCTTCAAGGATCGCATCACCGAGTTCGCGGCCG
AGTTCGGCAGCGAACTCGTGTTCCCGTGCGATGTCGCCGACGATGCGCAGATCGATGCGCTCTTCGCGTCGCTGAAGACG
CACTGGGATTCGCTCGACGGCCTCGTCCACTCGATCGGCTTCGCGCCGCGCGAGGCGATCGCGGGCGACTTCCTCGACGG
CCTCACGCGCGAGAACTTCCGCATCGCGCACGACATCTCCGCATACAGCTTCCCCGCGCTCGCGAAGGCGGCGCTGCCGA
TGCTGTCGGACGATGCGTCGCTGCTCACGCTGTCGTATCTCGGCGCGGAGCGGGCGATCCCGAACTACAACACGATGGGC
CTCGCGAAGGCGGCGCTCGAGGCGAGCGTGCGCTATCTCGCGGTGTCGCTCGGCGCGAAGGGCGTGCGCGTGAACGCGAT
CTCGGCGGGCCCGATCAAGACGCTCGCGGCAAGCGGCATCAAGAGCTTCGGCAAGATCCTCGATTTCGTCGAGAGCAACT
CGCCGCTCAAGCGCAACGTGACGATCGAGCAGGTCGGCAACGCGGGCGCGTTCCTGCTGTCGGACCTCGCGAGCGGCGTC
ACGGCCGAAGTCATGCACGTCGACTCGGGCTTCAACGCGGTGGTGGGCGGGATGGCCGGCCTCGAGGAATAA

Upstream 100 bases:

>100_bases
GAGGATGGCGATGTGAGAGAATTCTACCCATTCGCAACCGGTTGCAGCCAAACGCAGCCCGCCGACCGTGCGGGCGCGGG
CGCCACACATAGGAGAATTC

Downstream 100 bases:

>100_bases
GGCCGCCGGGCGCCGGCCCGGGCCCCGCGCCTTGACGCGGCTTCGCCCGGCGCCCCAAAAAAGCATCCCGCGCTCGCGAA
AGCGCGGGATTCGTCGGAGG

Product: enoyl-(acyl carrier protein) reductase

Products: NA

Alternate protein names: NADH-dependent enoyl-ACP reductase [H]

Number of amino acids: Translated: 263; Mature: 262

Protein sequence:

>263_residues
MGFLDGKRILLTGLLSNRSIAYGIAKACKREGAELAFTYVGDRFKDRITEFAAEFGSELVFPCDVADDAQIDALFASLKT
HWDSLDGLVHSIGFAPREAIAGDFLDGLTRENFRIAHDISAYSFPALAKAALPMLSDDASLLTLSYLGAERAIPNYNTMG
LAKAALEASVRYLAVSLGAKGVRVNAISAGPIKTLAASGIKSFGKILDFVESNSPLKRNVTIEQVGNAGAFLLSDLASGV
TAEVMHVDSGFNAVVGGMAGLEE

Sequences:

>Translated_263_residues
MGFLDGKRILLTGLLSNRSIAYGIAKACKREGAELAFTYVGDRFKDRITEFAAEFGSELVFPCDVADDAQIDALFASLKT
HWDSLDGLVHSIGFAPREAIAGDFLDGLTRENFRIAHDISAYSFPALAKAALPMLSDDASLLTLSYLGAERAIPNYNTMG
LAKAALEASVRYLAVSLGAKGVRVNAISAGPIKTLAASGIKSFGKILDFVESNSPLKRNVTIEQVGNAGAFLLSDLASGV
TAEVMHVDSGFNAVVGGMAGLEE
>Mature_262_residues
GFLDGKRILLTGLLSNRSIAYGIAKACKREGAELAFTYVGDRFKDRITEFAAEFGSELVFPCDVADDAQIDALFASLKTH
WDSLDGLVHSIGFAPREAIAGDFLDGLTRENFRIAHDISAYSFPALAKAALPMLSDDASLLTLSYLGAERAIPNYNTMGL
AKAALEASVRYLAVSLGAKGVRVNAISAGPIKTLAASGIKSFGKILDFVESNSPLKRNVTIEQVGNAGAFLLSDLASGVT
AEVMHVDSGFNAVVGGMAGLEE

Specific function: Fatty acid biosynthesis pathway; second reduction step. [C]

COG id: COG0623

COG function: function code I; Enoyl-[acyl-carrier-protein] reductase (NADH)

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family. FabI subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787545, Length=252, Percent_Identity=63.8888888888889, Blast_Score=335, Evalue=2e-93,
Organism=Escherichia coli, GI1789378, Length=255, Percent_Identity=30.9803921568627, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI87082100, Length=259, Percent_Identity=28.5714285714286, Blast_Score=69, Evalue=2e-13,
Organism=Escherichia coli, GI2367365, Length=256, Percent_Identity=28.90625, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI2367175, Length=250, Percent_Identity=26.4, Blast_Score=64, Evalue=7e-12,
Organism=Escherichia coli, GI1787905, Length=250, Percent_Identity=24.8, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR014358
- InterPro:   IPR002347
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: =1.3.1.9 [H]

Molecular weight: Translated: 27771; Mature: 27640

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGFLDGKRILLTGLLSNRSIAYGIAKACKREGAELAFTYVGDRFKDRITEFAAEFGSELV
CCCCCCCCEEEEHHHCCCHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHCCCEE
FPCDVADDAQIDALFASLKTHWDSLDGLVHSIGFAPREAIAGDFLDGLTRENFRIAHDIS
EECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCEEEEECCC
AYSFPALAKAALPMLSDDASLLTLSYLGAERAIPNYNTMGLAKAALEASVRYLAVSLGAK
HHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCC
GVRVNAISAGPIKTLAASGIKSFGKILDFVESNSPLKRNVTIEQVGNAGAFLLSDLASGV
CEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCHHHHHHHHHCCC
TAEVMHVDSGFNAVVGGMAGLEE
EEEEEEECCCCHHHHHHHCCCCC
>Mature Secondary Structure 
GFLDGKRILLTGLLSNRSIAYGIAKACKREGAELAFTYVGDRFKDRITEFAAEFGSELV
CCCCCCCEEEEHHHCCCHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHCCCEE
FPCDVADDAQIDALFASLKTHWDSLDGLVHSIGFAPREAIAGDFLDGLTRENFRIAHDIS
EECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCEEEEECCC
AYSFPALAKAALPMLSDDASLLTLSYLGAERAIPNYNTMGLAKAALEASVRYLAVSLGAK
HHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCC
GVRVNAISAGPIKTLAASGIKSFGKILDFVESNSPLKRNVTIEQVGNAGAFLLSDLASGV
CEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCHHHHHHHHHCCC
TAEVMHVDSGFNAVVGGMAGLEE
EEEEEEECCCCHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 10710307 [H]