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The map label for this gene is psdht [H]

Identifier: 237812721

GI number: 237812721

Start: 2419850

End: 2420881

Strand: Direct

Name: psdht [H]

Synonym: GBP346_A2474

Alternate gene names: 237812721

Gene position: 2419850-2420881 (Clockwise)

Preceding gene: 237812720

Following gene: 237812723

Centisome position: 59.04

GC content: 70.25

Gene sequence:

>1032_bases
ATGTCGACCTCACCCAACCGCCCCGCTCATCTGACGATCAACGGCGAGCCGATCCCGACGCTCGACGAGATCGCCGCGCA
ACACTTCGCGCTCACGCCGTGGGTCATGCGCACGCCGGTGTTCGAGCGCGCGGATCTGCCGTCGCTCGACAACACGCACG
TGAACTTCAAGTTCGAGCTGCTGCAGGCGAGCGGCAGCTTCAAGGCGCGCGGCGCGTTCACGAACCTGCTCGCGCTCGGC
GAGGACAGGAAGAGCGTGGGCGTCACCTGCGTGTCGGCCGGCAATCACGCGGCGGCCGTCGCGTATGCGGCGATGCGACT
CGGCACGAGCGCGAAGGTCGTGATGTTCGACACCGCGAGCCCGGCGCGCGTCGCGCTCTGCCGCCACTACCGCGCGGAGG
TCGTGATGGCGTCGAGCCCCGCCGAGGCGTTCGAGATCGTGCGGCGCATCGAAGCCGAGGAAGGCCGCTTCTTCGTGCAT
CCGTTCAACGGCTACCACACGATTCTCGGCACCGCGACGCTCGGCTACGAATGGGCGACGCAGACGCCCGACCTCGATGC
GGTGATCGTGCCGATCGGCGGGGGCGGCCTCGCCGCGGGCACGTCGACCGCGCTGCGCCTCGCGAACCCCCGCGTGCACG
TGTACGGCGTCGAGCCGGAAGGCGCGGATGCGATGAGCCGCAGCTTCGCCGCGAATCACCCGGTCCGCATGGGCGCGATG
CACACGATCGCCGATTCGCTGATGGCGCCGCACACCGAGGAGTACAGCTACGAGCTGTGTCGTCGCCACGTCGACCGGAT
CGTCACCGTGTCGGACGACGCGCTGCGCGCGGCGATGCTCTATCTGTTCGCGCATCTGAAGGTCGCGGTCGAGCCCGCGT
GCGCGGCCGCGACGGCCGCCCTCCTCGGGCCGCTGCGCGAGTCGCTGCAGGGCAGGCGCGTCGGCGTGCTGCTGTCGGGC
ACCAACATCGATTCGACCGCGCTCGCCGCGCATCTCGAGCACGCGCGGCTGCATCGGGCCGCGCAACCGTAG

Upstream 100 bases:

>100_bases
TTCGTGCGCAAGCTCGTGCAGGGCATGACGCTCGCGTAAGCCCGCGCCGCACTCCGTTCCGGCCGTCCGCTCGGCCGCCG
TCATCGATACGCGCACGCCC

Downstream 100 bases:

>100_bases
CGCCGGTTATCCCCAGTTCGTGTTGAAGAGCATGTTGAAAAGCCGCGGACAAGCGTGCTAACGCGTTGATCGCGCGACGT
TTTCGGAAGCCGCCGCGAAA

Product: phenylserine dehydratase

Products: 2-oxobutanoate; NH3

Alternate protein names: NA

Number of amino acids: Translated: 343; Mature: 342

Protein sequence:

>343_residues
MSTSPNRPAHLTINGEPIPTLDEIAAQHFALTPWVMRTPVFERADLPSLDNTHVNFKFELLQASGSFKARGAFTNLLALG
EDRKSVGVTCVSAGNHAAAVAYAAMRLGTSAKVVMFDTASPARVALCRHYRAEVVMASSPAEAFEIVRRIEAEEGRFFVH
PFNGYHTILGTATLGYEWATQTPDLDAVIVPIGGGGLAAGTSTALRLANPRVHVYGVEPEGADAMSRSFAANHPVRMGAM
HTIADSLMAPHTEEYSYELCRRHVDRIVTVSDDALRAAMLYLFAHLKVAVEPACAAATAALLGPLRESLQGRRVGVLLSG
TNIDSTALAAHLEHARLHRAAQP

Sequences:

>Translated_343_residues
MSTSPNRPAHLTINGEPIPTLDEIAAQHFALTPWVMRTPVFERADLPSLDNTHVNFKFELLQASGSFKARGAFTNLLALG
EDRKSVGVTCVSAGNHAAAVAYAAMRLGTSAKVVMFDTASPARVALCRHYRAEVVMASSPAEAFEIVRRIEAEEGRFFVH
PFNGYHTILGTATLGYEWATQTPDLDAVIVPIGGGGLAAGTSTALRLANPRVHVYGVEPEGADAMSRSFAANHPVRMGAM
HTIADSLMAPHTEEYSYELCRRHVDRIVTVSDDALRAAMLYLFAHLKVAVEPACAAATAALLGPLRESLQGRRVGVLLSG
TNIDSTALAAHLEHARLHRAAQP
>Mature_342_residues
STSPNRPAHLTINGEPIPTLDEIAAQHFALTPWVMRTPVFERADLPSLDNTHVNFKFELLQASGSFKARGAFTNLLALGE
DRKSVGVTCVSAGNHAAAVAYAAMRLGTSAKVVMFDTASPARVALCRHYRAEVVMASSPAEAFEIVRRIEAEEGRFFVHP
FNGYHTILGTATLGYEWATQTPDLDAVIVPIGGGGLAAGTSTALRLANPRVHVYGVEPEGADAMSRSFAANHPVRMGAMH
TIADSLMAPHTEEYSYELCRRHVDRIVTVSDDALRAAMLYLFAHLKVAVEPACAAATAALLGPLRESLQGRRVGVLLSGT
NIDSTALAAHLEHARLHRAAQP

Specific function: Acts On Both Serine And Threonine, And Properly Considered As A Hydroxy Amino Acid Deaminase. [C]

COG id: COG1171

COG function: function code E; Threonine dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI11345492, Length=321, Percent_Identity=29.9065420560748, Blast_Score=142, Evalue=5e-34,
Organism=Escherichia coli, GI1789505, Length=271, Percent_Identity=38.7453874538745, Blast_Score=145, Evalue=4e-36,
Organism=Escherichia coli, GI1790207, Length=306, Percent_Identity=33.0065359477124, Blast_Score=131, Evalue=7e-32,
Organism=Caenorhabditis elegans, GI71991565, Length=312, Percent_Identity=33.974358974359, Blast_Score=161, Evalue=4e-40,
Organism=Caenorhabditis elegans, GI17537387, Length=317, Percent_Identity=33.4384858044164, Blast_Score=134, Evalue=6e-32,
Organism=Caenorhabditis elegans, GI17508781, Length=315, Percent_Identity=27.3015873015873, Blast_Score=121, Evalue=4e-28,
Organism=Saccharomyces cerevisiae, GI6322631, Length=309, Percent_Identity=35.5987055016181, Blast_Score=160, Evalue=2e-40,
Organism=Saccharomyces cerevisiae, GI6320930, Length=271, Percent_Identity=30.6273062730627, Blast_Score=93, Evalue=5e-20,
Organism=Saccharomyces cerevisiae, GI6319788, Length=284, Percent_Identity=26.4084507042254, Blast_Score=84, Evalue=3e-17,
Organism=Drosophila melanogaster, GI21355833, Length=333, Percent_Identity=33.3333333333333, Blast_Score=149, Evalue=4e-36,
Organism=Drosophila melanogaster, GI24645328, Length=214, Percent_Identity=34.5794392523364, Blast_Score=99, Evalue=6e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001926 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: 4.3.1.19

Molecular weight: Translated: 36794; Mature: 36663

Theoretical pI: Translated: 7.11; Mature: 7.11

Prosite motif: PS00165 DEHYDRATASE_SER_THR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTSPNRPAHLTINGEPIPTLDEIAAQHFALTPWVMRTPVFERADLPSLDNTHVNFKFEL
CCCCCCCCEEEEECCCCCCCHHHHHHHHHCCCCHHHHCCCCCCCCCCCCCCCEEEEEEEE
LQASGSFKARGAFTNLLALGEDRKSVGVTCVSAGNHAAAVAYAAMRLGTSAKVVMFDTAS
EECCCCCEECCHHHHHHHCCCCCCCCCEEEEECCCCHHHHHHHHHHCCCCCEEEEEECCC
PARVALCRHYRAEVVMASSPAEAFEIVRRIEAEEGRFFVHPFNGYHTILGTATLGYEWAT
CHHHHHHHHHHEEEEEECCCHHHHHHHHHHHHCCCEEEEEECCCEEEEEEEEEECEEECC
QTPDLDAVIVPIGGGGLAAGTSTALRLANPRVHVYGVEPEGADAMSRSFAANHPVRMGAM
CCCCCCEEEEEECCCCCCCCCCCEEEECCCEEEEEEECCCCCHHHHHHHHCCCCCHHHHH
HTIADSLMAPHTEEYSYELCRRHVDRIVTVSDDALRAAMLYLFAHLKVAVEPACAAATAA
HHHHHHHHCCCCHHHHHHHHHHHHHHEEEECCHHHHHHHHHHHHHHHHEECHHHHHHHHH
LLGPLRESLQGRRVGVLLSGTNIDSTALAAHLEHARLHRAAQP
HHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
STSPNRPAHLTINGEPIPTLDEIAAQHFALTPWVMRTPVFERADLPSLDNTHVNFKFEL
CCCCCCCEEEEECCCCCCCHHHHHHHHHCCCCHHHHCCCCCCCCCCCCCCCEEEEEEEE
LQASGSFKARGAFTNLLALGEDRKSVGVTCVSAGNHAAAVAYAAMRLGTSAKVVMFDTAS
EECCCCCEECCHHHHHHHCCCCCCCCCEEEEECCCCHHHHHHHHHHCCCCCEEEEEECCC
PARVALCRHYRAEVVMASSPAEAFEIVRRIEAEEGRFFVHPFNGYHTILGTATLGYEWAT
CHHHHHHHHHHEEEEEECCCHHHHHHHHHHHHCCCEEEEEECCCEEEEEEEEEECEEECC
QTPDLDAVIVPIGGGGLAAGTSTALRLANPRVHVYGVEPEGADAMSRSFAANHPVRMGAM
CCCCCCEEEEEECCCCCCCCCCCEEEECCCEEEEEEECCCCCHHHHHHHHCCCCCHHHHH
HTIADSLMAPHTEEYSYELCRRHVDRIVTVSDDALRAAMLYLFAHLKVAVEPACAAATAA
HHHHHHHHCCCCHHHHHHHHHHHHHHEEEECCHHHHHHHHHHHHHHHHEECHHHHHHHHH
LLGPLRESLQGRRVGVLLSGTNIDSTALAAHLEHARLHRAAQP
HHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: AMP; L-Val; pyridoxal 5'-phosphate [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 91 {L-Thr}} 70 {L-Thr}} 11 {L-Thr}} 40 {L-Ser}} 5 {L-Ser}} [C]

Substrates: L-threonine

Specific reaction: L-threonine = 2-oxobutanoate + NH3

General reaction: Deamination; elimination of NH alphabeta-position of amino acid [C]

Inhibitor: Ile [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12596884; 8743570 [H]