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The map label for this gene is tesA [H]

Identifier: 237812669

GI number: 237812669

Start: 2374666

End: 2375346

Strand: Direct

Name: tesA [H]

Synonym: GBP346_A2419

Alternate gene names: 237812669

Gene position: 2374666-2375346 (Clockwise)

Preceding gene: 237812667

Following gene: 237812674

Centisome position: 57.94

GC content: 70.78

Gene sequence:

>681_bases
TTGAGCATGAAACGCACAGTTCACTGGAAGGCCGGCGCGACGCTCGCCGTGGTCGCGACGTTCGCCGGCCCGGCATCCGC
CGCCACGAATGCAGCGCCCGCGCCGAACGCCGACGGCGGCAAGCCGGTGATCGTCGTGCTCGGCGACAGCCTGTCGGCCG
AATACGGCCTGCCGCGCGATACGGGCTGGGTCGCGCTGATGCGCGCGCGCCTCGCGGCCGAGCGAATCGATTATAGCGTC
GCGAACGCGAGCATCTCCGGCGACACGACGAGCGGCGGCCGCGCGCGGCTGCCCGCGGTGCTCGCACGGCTCAAGCCGGC
CGTCGTCGTCGTCGAACTCGGCGCGAACGACGCGCTGCGCGGCGTGCCGCTCGCCGCGACCGAAGGCAACCTGCGCGAGA
TCGTCGCGCAGGCGCGGCAGGCGCGCGCGCAGGTGCTGCTCGTCGGCATGTACGTGCCGTCGAACTACGGCCCGGACTAC
ACGCAAAAGTTCCGCGGCGTCTACACGGGGCTGTCCAAGGAGATGCGCGTGCCGCTCGTGCCGTTCCTGCTCGCCGGCAT
CGAGAACAAGCCCGACATGTTCCAGGCCGACCAGATCCATCCGACGCAGGCCGCGCAGCGCCAGTTGCTCGACAACGTAT
GGCCGGCCCTCAAGCCGCTGCTGCGGACGTCATCGCACTGA

Upstream 100 bases:

>100_bases
GCGAACCGTCAGGTCGATGCCAGCGAGAATCGTCAGCTCGCCGGCGGCGTCGGCGACACGCTTGCACACATTCCGTACTT
CGATGATTGGATCGGTAATC

Downstream 100 bases:

>100_bases
TCGCGCCGTGCCGGCATCTCGGTACTTCAGTACTTCGGTGCTTCGGTACTGCAACGCTTGATATGTCGATCCGCCGGCGG
TGCCGGGCCGGCCGATACCA

Product: putative acyl-CoA thioesterase

Products: NA

Alternate protein names: Lecithinase B; Lysophospholipase L1; Protease I [H]

Number of amino acids: Translated: 226; Mature: 225

Protein sequence:

>226_residues
MSMKRTVHWKAGATLAVVATFAGPASAATNAAPAPNADGGKPVIVVLGDSLSAEYGLPRDTGWVALMRARLAAERIDYSV
ANASISGDTTSGGRARLPAVLARLKPAVVVVELGANDALRGVPLAATEGNLREIVAQARQARAQVLLVGMYVPSNYGPDY
TQKFRGVYTGLSKEMRVPLVPFLLAGIENKPDMFQADQIHPTQAAQRQLLDNVWPALKPLLRTSSH

Sequences:

>Translated_226_residues
MSMKRTVHWKAGATLAVVATFAGPASAATNAAPAPNADGGKPVIVVLGDSLSAEYGLPRDTGWVALMRARLAAERIDYSV
ANASISGDTTSGGRARLPAVLARLKPAVVVVELGANDALRGVPLAATEGNLREIVAQARQARAQVLLVGMYVPSNYGPDY
TQKFRGVYTGLSKEMRVPLVPFLLAGIENKPDMFQADQIHPTQAAQRQLLDNVWPALKPLLRTSSH
>Mature_225_residues
SMKRTVHWKAGATLAVVATFAGPASAATNAAPAPNADGGKPVIVVLGDSLSAEYGLPRDTGWVALMRARLAAERIDYSVA
NASISGDTTSGGRARLPAVLARLKPAVVVVELGANDALRGVPLAATEGNLREIVAQARQARAQVLLVGMYVPSNYGPDYT
QKFRGVYTGLSKEMRVPLVPFLLAGIENKPDMFQADQIHPTQAAQRQLLDNVWPALKPLLRTSSH

Specific function: Hydrolyzes only long chain acyl thioesters (C12-C18). Specificity similar to chymotrypsin [H]

COG id: COG2755

COG function: function code E; Lysophospholipase L1 and related esterases

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDSL' lipolytic enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1786702, Length=225, Percent_Identity=36.8888888888889, Blast_Score=141, Evalue=4e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013830
- InterPro:   IPR013831
- InterPro:   IPR001087
- InterPro:   IPR008265 [H]

Pfam domain/function: PF00657 Lipase_GDSL [H]

EC number: =3.1.1.5 [H]

Molecular weight: Translated: 23962; Mature: 23831

Theoretical pI: Translated: 10.35; Mature: 10.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSMKRTVHWKAGATLAVVATFAGPASAATNAAPAPNADGGKPVIVVLGDSLSAEYGLPRD
CCCCCEEEECCCCEEEEEEECCCCCHHCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCC
TGWVALMRARLAAERIDYSVANASISGDTTSGGRARLPAVLARLKPAVVVVELGANDALR
CHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCEEEEEEECCCCCCC
GVPLAATEGNLREIVAQARQARAQVLLVGMYVPSNYGPDYTQKFRGVYTGLSKEMRVPLV
CCCEECCCCHHHHHHHHHHHHHHEEEEEEEECCCCCCCCHHHHHHHHHHCCCCCCCCCHH
PFLLAGIENKPDMFQADQIHPTQAAQRQLLDNVWPALKPLLRTSSH
HHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SMKRTVHWKAGATLAVVATFAGPASAATNAAPAPNADGGKPVIVVLGDSLSAEYGLPRD
CCCCEEEECCCCEEEEEEECCCCCHHCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCC
TGWVALMRARLAAERIDYSVANASISGDTTSGGRARLPAVLARLKPAVVVVELGANDALR
CHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCEEEEEEECCCCCCC
GVPLAATEGNLREIVAQARQARAQVLLVGMYVPSNYGPDYTQKFRGVYTGLSKEMRVPLV
CCCEECCCCHHHHHHHHHHHHHHEEEEEEEECCCCCCCCHHHHHHHHHHCCCCCCCCCHH
PFLLAGIENKPDMFQADQIHPTQAAQRQLLDNVWPALKPLLRTSSH
HHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]