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The map label for this gene is nolF [H]

Identifier: 237812485

GI number: 237812485

Start: 2194771

End: 2195982

Strand: Direct

Name: nolF [H]

Synonym: GBP346_A2234

Alternate gene names: 237812485

Gene position: 2194771-2195982 (Clockwise)

Preceding gene: 237812483

Following gene: 237812486

Centisome position: 53.55

GC content: 72.19

Gene sequence:

>1212_bases
TTGTTTTGGTTTTTTGCCGCGCGCGACGCCGGGCAAGCGCATCCGGCTTGCCGGTTTTTCGGTTTTTTTCCGTTTTCTCC
TGCCCGAGGGGTCACCGTGAATCGTTCCGGTTCTCGCGCCGCGCTGCTGATCGGCGTCGCGCTCATCGCCGCCGCCTGTC
ATCGCAAGGAAGCCGCGCCGAGCGCGCCGCGCCCCGTCGTCGCAGTGCCGGCGCAGGCGGACGGCGCGGCCGCCGCCGTG
TCGCTGCCCGGCGAGATCCAGCCGCGCTACGCCACGCCGCTGTCGTTTCGCATCGCCGGCAAGCTCGTCGAGCGCAAGGT
GCGCCTCGGCGACATCGTGAAGAAAGGCCAGGTCGTCGCGCTGCTCGATACGTCCGACGTCGCGCGCAACGCGGCCAGCG
CGCAGGCGCAGCTCGACGCCGCGACGCACGCTCTGACGTTCGCGCAGCAGCAGCGCGAGCGCGATCGCGCGCAGGCGCGC
GAGAACCTGATCGCGCCCGCGCAGCTCGAGCAGACCGAGAACGCGTACGCCTCCGCGCGCGCGCAGCGCGATCAGGCCGC
GCAGCAGCTCGCGCTCGCGAAGAACCAGTTGCAGTACGCGACGCTCGTCGCCGATCACGCGGGCTACATCACCGCCGAGC
AGGCCGACACCGGGCAGAACGTGTCCGCCGGCCAGCCGGTCTATCAGCTCGCGTGGTCGGGCGACATCGACGTCGTCACC
GACGCGCCGGAAAGCGAACTGCCGCGCCTCGCGCCGGGCCGCACGGCGCGCGTCACGCTGCCGGCGCTGCCCGGCCGCGC
GTTCGACGCGCGCGTGCGCGAGATCGCGCCCGCCGCCGATCCGCAAAGCCGCACGTACCGCGTGAAGCTCACGCTCGCGC
AGCCCGATGCGGCGGTGCGGCTCGGGATGACCGCGAACGTCGCGCTCTCGCCCGATGCGCCGAGCGCCGCGAACGCGCAG
CCGCGCTTCACGCTGCCGTCGACGGCGCTCTTTCATGCCGGCAACGCGCCCGCGCTGTGGATCGTGCGCAAGCAGGACGA
CACGCTCGAGCTGCGGCGCGTCGACGTCGTGCGCTACAACGAGCGCACGGTGACCGTGTCGGGCGGAATTCAGCCGGGCG
AGCGCGTCGTGCTGCAAGGCGTGCACACGGTGAGCGCGGGCGAGAAGGTGCGCGCGGTGCCGCCGCTTCATCCGGAGGAC
GTCGCGTCATGA

Upstream 100 bases:

>100_bases
TAGCATCTTCCCGCGAATCGAAACACCTTTGCATCCAGATACAACCCTGTATTAGAATCCGACCAGGTTTTTGTAAGCAA
GTCATCAACGTGCGCGCCGA

Downstream 100 bases:

>100_bases
CGGCCGGCCGAGAAGAAGGCGGACGCTTCAACCTGTCCGCATGGGCGCTGCGCCATCAGGCGCTCGTCGTCTACCTGATC
GCGCTCGCGACGATCGCGGG

Product: efflux transporter, RND family, MFP subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 403; Mature: 403

Protein sequence:

>403_residues
MFWFFAARDAGQAHPACRFFGFFPFSPARGVTVNRSGSRAALLIGVALIAAACHRKEAAPSAPRPVVAVPAQADGAAAAV
SLPGEIQPRYATPLSFRIAGKLVERKVRLGDIVKKGQVVALLDTSDVARNAASAQAQLDAATHALTFAQQQRERDRAQAR
ENLIAPAQLEQTENAYASARAQRDQAAQQLALAKNQLQYATLVADHAGYITAEQADTGQNVSAGQPVYQLAWSGDIDVVT
DAPESELPRLAPGRTARVTLPALPGRAFDARVREIAPAADPQSRTYRVKLTLAQPDAAVRLGMTANVALSPDAPSAANAQ
PRFTLPSTALFHAGNAPALWIVRKQDDTLELRRVDVVRYNERTVTVSGGIQPGERVVLQGVHTVSAGEKVRAVPPLHPED
VAS

Sequences:

>Translated_403_residues
MFWFFAARDAGQAHPACRFFGFFPFSPARGVTVNRSGSRAALLIGVALIAAACHRKEAAPSAPRPVVAVPAQADGAAAAV
SLPGEIQPRYATPLSFRIAGKLVERKVRLGDIVKKGQVVALLDTSDVARNAASAQAQLDAATHALTFAQQQRERDRAQAR
ENLIAPAQLEQTENAYASARAQRDQAAQQLALAKNQLQYATLVADHAGYITAEQADTGQNVSAGQPVYQLAWSGDIDVVT
DAPESELPRLAPGRTARVTLPALPGRAFDARVREIAPAADPQSRTYRVKLTLAQPDAAVRLGMTANVALSPDAPSAANAQ
PRFTLPSTALFHAGNAPALWIVRKQDDTLELRRVDVVRYNERTVTVSGGIQPGERVVLQGVHTVSAGEKVRAVPPLHPED
VAS
>Mature_403_residues
MFWFFAARDAGQAHPACRFFGFFPFSPARGVTVNRSGSRAALLIGVALIAAACHRKEAAPSAPRPVVAVPAQADGAAAAV
SLPGEIQPRYATPLSFRIAGKLVERKVRLGDIVKKGQVVALLDTSDVARNAASAQAQLDAATHALTFAQQQRERDRAQAR
ENLIAPAQLEQTENAYASARAQRDQAAQQLALAKNQLQYATLVADHAGYITAEQADTGQNVSAGQPVYQLAWSGDIDVVT
DAPESELPRLAPGRTARVTLPALPGRAFDARVREIAPAADPQSRTYRVKLTLAQPDAAVRLGMTANVALSPDAPSAANAQ
PRFTLPSTALFHAGNAPALWIVRKQDDTLELRRVDVVRYNERTVTVSGGIQPGERVVLQGVHTVSAGEKVRAVPPLHPED
VAS

Specific function: Involved in the production of Medicago-specific nodulation signal molecule [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI87081796, Length=304, Percent_Identity=24.3421052631579, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 42891; Mature: 42891

Theoretical pI: Translated: 9.95; Mature: 9.95

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFWFFAARDAGQAHPACRFFGFFPFSPARGVTVNRSGSRAALLIGVALIAAACHRKEAAP
CEEEEEECCCCCCCCCEEEEEECCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHCCC
SAPRPVVAVPAQADGAAAAVSLPGEIQPRYATPLSFRIAGKLVERKVRLGDIVKKGQVVA
CCCCCEEEECCCCCCCEEEEECCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHCCCCEEE
LLDTSDVARNAASAQAQLDAATHALTFAQQQRERDRAQARENLIAPAQLEQTENAYASAR
EEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
AQRDQAAQQLALAKNQLQYATLVADHAGYITAEQADTGQNVSAGQPVYQLAWSGDIDVVT
HHHHHHHHHHHHHHHHHHEEEEEECCCCEEEECCCCCCCCCCCCCCEEEEEECCCEEEEE
DAPESELPRLAPGRTARVTLPALPGRAFDARVREIAPAADPQSRTYRVKLTLAQPDAAVR
CCCHHHCCCCCCCCEEEEEECCCCCCHHHHHHHHHCCCCCCCCCEEEEEEEEECCCCEEE
LGMTANVALSPDAPSAANAQPRFTLPSTALFHAGNAPALWIVRKQDDTLELRRVDVVRYN
ECEEEEEEECCCCCCCCCCCCCEECCCHHEEECCCCCEEEEEECCCCCEEEEEEEEEEEC
ERTVTVSGGIQPGERVVLQGVHTVSAGEKVRAVPPLHPEDVAS
CEEEEEECCCCCCCCEEEEECEEECCCCCEECCCCCCHHHCCC
>Mature Secondary Structure
MFWFFAARDAGQAHPACRFFGFFPFSPARGVTVNRSGSRAALLIGVALIAAACHRKEAAP
CEEEEEECCCCCCCCCEEEEEECCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHCCC
SAPRPVVAVPAQADGAAAAVSLPGEIQPRYATPLSFRIAGKLVERKVRLGDIVKKGQVVA
CCCCCEEEECCCCCCCEEEEECCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHCCCCEEE
LLDTSDVARNAASAQAQLDAATHALTFAQQQRERDRAQARENLIAPAQLEQTENAYASAR
EEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
AQRDQAAQQLALAKNQLQYATLVADHAGYITAEQADTGQNVSAGQPVYQLAWSGDIDVVT
HHHHHHHHHHHHHHHHHHEEEEEECCCCEEEECCCCCCCCCCCCCCEEEEEECCCEEEEE
DAPESELPRLAPGRTARVTLPALPGRAFDARVREIAPAADPQSRTYRVKLTLAQPDAAVR
CCCHHHCCCCCCCCEEEEEECCCCCCHHHHHHHHHCCCCCCCCCEEEEEEEEECCCCEEE
LGMTANVALSPDAPSAANAQPRFTLPSTALFHAGNAPALWIVRKQDDTLELRRVDVVRYN
ECEEEEEEECCCCCCCCCCCCCEECCCHHEEECCCCCEEEEEECCCCCEEEEEEEEEEEC
ERTVTVSGGIQPGERVVLQGVHTVSAGEKVRAVPPLHPEDVAS
CEEEEEECCCCCCCCEEEEECEEECCCCCEECCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1909418; 11481432 [H]