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The map label for this gene is ntaB [H]

Identifier: 237812384

GI number: 237812384

Start: 2108930

End: 2109886

Strand: Reverse

Name: ntaB [H]

Synonym: GBP346_A2132

Alternate gene names: 237812384

Gene position: 2109886-2108930 (Counterclockwise)

Preceding gene: 237812385

Following gene: 237812383

Centisome position: 51.48

GC content: 63.22

Gene sequence:

>957_bases
ATGACTGAAACGATCAAAGAACCCGCGATCGATATCGCCGAATTTCGTCGCGCGCTCGGCGCGTTCGTGACGGGCGTGAC
GGTTGTTACGACGATTCAGCCCGACGGCTCGCCGCGCGGCTTCACGGCGAATTCGTTCACGTCGGTCTCGCTCGATCCGC
CGCTGATCCTCGTTTGCATCGCGAAGACAGCGTCGAGCTATGCGGTGTTTTCGCAGACGAAGCACTTCGCGATCAGCGTG
CTGGCCGAAGACCAGAAGGCCGTATCGGGCGTCTTCGCCTCGAAGGCCGCCGACAAGTTCTCGCAAGTGGCCTGGCATCG
GCGCGTGACAGGTGCGCCCGTGATGGAGGGTGCCGCCGCGAGTTTCGACTGCGCGACGCACGAAGTTATCGATGCCGGCG
ATCACATCATTTTGATCGGCCGCGTCCTAGACTTCATCCATACGGCTTCGTCTCCGCTCGGCTATTGCCGCGGCGCCTAT
GTGAATTTCAGCCTGTCGCTCGATGCATTGGCCGTAGCGGGGCCGCGTGCGCACGTGGGTGCGATCCTGGAACATCGCGA
CGGCCTTGTGCTGCTCGATACGCCGCGAGGTCTGCAATTGCCGACGGGAGCCAAACTCAAACCGGAAAGCGATGGCGCGA
GTCTGCTCGGTGTCCTCGGTCAGCTGGGCCTCAAAGTGCATCTGGACTTCCTGTTCGCCGTATTCGAAGCAGGCGGCGGC
CCGGCGCCGGGCGTGCAGATCTACTACCGCGGCCGCGTGAAGAACGACATCTTGCCGCAATCGGGCGGCCCGATTCGAAT
TGTCCCATTGCCGCAGATTCGGTGGGACGCGATCCGCGACGACGCCGTGCGTTCGATGCTGCAGCGCTACGTGCGTGAGC
GCAGCGAGGACGCCTTCGGGATCTATGTAGGCGATACCGAGACCGGAACGGTGCAGCCGCTGGCGCCGGCTCACTGA

Upstream 100 bases:

>100_bases
ACCAACCCCGCGCAAGTGAACGAACGGTTGCTCGAATTCTTGCTGAGCGCGTCGGCCGTCAGCACGGTGGGCGATCGGCA
CGGCGACGCCGGAGGATGCC

Downstream 100 bases:

>100_bases
ACTGAACGGCACTGCACGAATTCATTAATCGAGAGCGATTGACGCGCGGCGAATGGTGCGCTGCGGTAACGAGGTCACCG
TGCTGCCCGCGATAGGAGAT

Product: nitrilotriacetate monooxygenase

Products: NAD(+); reduced flavin [C]

Alternate protein names: NTA monooxygenase component B; NTA-MO B [H]

Number of amino acids: Translated: 318; Mature: 317

Protein sequence:

>318_residues
MTETIKEPAIDIAEFRRALGAFVTGVTVVTTIQPDGSPRGFTANSFTSVSLDPPLILVCIAKTASSYAVFSQTKHFAISV
LAEDQKAVSGVFASKAADKFSQVAWHRRVTGAPVMEGAAASFDCATHEVIDAGDHIILIGRVLDFIHTASSPLGYCRGAY
VNFSLSLDALAVAGPRAHVGAILEHRDGLVLLDTPRGLQLPTGAKLKPESDGASLLGVLGQLGLKVHLDFLFAVFEAGGG
PAPGVQIYYRGRVKNDILPQSGGPIRIVPLPQIRWDAIRDDAVRSMLQRYVRERSEDAFGIYVGDTETGTVQPLAPAH

Sequences:

>Translated_318_residues
MTETIKEPAIDIAEFRRALGAFVTGVTVVTTIQPDGSPRGFTANSFTSVSLDPPLILVCIAKTASSYAVFSQTKHFAISV
LAEDQKAVSGVFASKAADKFSQVAWHRRVTGAPVMEGAAASFDCATHEVIDAGDHIILIGRVLDFIHTASSPLGYCRGAY
VNFSLSLDALAVAGPRAHVGAILEHRDGLVLLDTPRGLQLPTGAKLKPESDGASLLGVLGQLGLKVHLDFLFAVFEAGGG
PAPGVQIYYRGRVKNDILPQSGGPIRIVPLPQIRWDAIRDDAVRSMLQRYVRERSEDAFGIYVGDTETGTVQPLAPAH
>Mature_317_residues
TETIKEPAIDIAEFRRALGAFVTGVTVVTTIQPDGSPRGFTANSFTSVSLDPPLILVCIAKTASSYAVFSQTKHFAISVL
AEDQKAVSGVFASKAADKFSQVAWHRRVTGAPVMEGAAASFDCATHEVIDAGDHIILIGRVLDFIHTASSPLGYCRGAYV
NFSLSLDALAVAGPRAHVGAILEHRDGLVLLDTPRGLQLPTGAKLKPESDGASLLGVLGQLGLKVHLDFLFAVFEAGGGP
APGVQIYYRGRVKNDILPQSGGPIRIVPLPQIRWDAIRDDAVRSMLQRYVRERSEDAFGIYVGDTETGTVQPLAPAH

Specific function: Hydroxylation of nitrilotriacetate [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI87081819, Length=147, Percent_Identity=33.3333333333333, Blast_Score=89, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002563
- InterPro:   IPR012349
- InterPro:   IPR009002
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01613 Flavin_Reduct [H]

EC number: 1.6.8.- [C]

Molecular weight: Translated: 33856; Mature: 33725

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTETIKEPAIDIAEFRRALGAFVTGVTVVTTIQPDGSPRGFTANSFTSVSLDPPLILVCI
CCCCCCCCCCCHHHHHHHHHHHHCCEEEEEEECCCCCCCCCCCCCEEEEECCCCEEEEEE
AKTASSYAVFSQTKHFAISVLAEDQKAVSGVFASKAADKFSQVAWHRRVTGAPVMEGAAA
ECCCCCEEEHHCCCEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
SFDCATHEVIDAGDHIILIGRVLDFIHTASSPLGYCRGAYVNFSLSLDALAVAGPRAHVG
CCCCCHHHHHCCCCCEEEHHHHHHHHHHCCCCCHHHCCEEEEEEEEECEEEECCCHHHHH
AILEHRDGLVLLDTPRGLQLPTGAKLKPESDGASLLGVLGQLGLKVHLDFLFAVFEAGGG
HHHCCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHCCCC
PAPGVQIYYRGRVKNDILPQSGGPIRIVPLPQIRWDAIRDDAVRSMLQRYVRERSEDAFG
CCCCEEEEECCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCEEE
IYVGDTETGTVQPLAPAH
EEECCCCCCCCCCCCCCC
>Mature Secondary Structure 
TETIKEPAIDIAEFRRALGAFVTGVTVVTTIQPDGSPRGFTANSFTSVSLDPPLILVCI
CCCCCCCCCCHHHHHHHHHHHHCCEEEEEEECCCCCCCCCCCCCEEEEECCCCEEEEEE
AKTASSYAVFSQTKHFAISVLAEDQKAVSGVFASKAADKFSQVAWHRRVTGAPVMEGAAA
ECCCCCEEEHHCCCEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
SFDCATHEVIDAGDHIILIGRVLDFIHTASSPLGYCRGAYVNFSLSLDALAVAGPRAHVG
CCCCCHHHHHCCCCCEEEHHHHHHHHHHCCCCCHHHCCEEEEEEEEECEEEECCCHHHHH
AILEHRDGLVLLDTPRGLQLPTGAKLKPESDGASLLGVLGQLGLKVHLDFLFAVFEAGGG
HHHCCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHCCCC
PAPGVQIYYRGRVKNDILPQSGGPIRIVPLPQIRWDAIRDDAVRSMLQRYVRERSEDAFG
CCCCEEEEECCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCEEE
IYVGDTETGTVQPLAPAH
EEECCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; flavin [C]

Specific reaction: NADH + flavin = NAD(+) + reduced flavin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8892809; 9023192; 1735711 [H]