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The map label for this gene is gutB [H]

Identifier: 237812205

GI number: 237812205

Start: 1889610

End: 1890701

Strand: Direct

Name: gutB [H]

Synonym: GBP346_A1951

Alternate gene names: 237812205

Gene position: 1889610-1890701 (Clockwise)

Preceding gene: 237812204

Following gene: 237812206

Centisome position: 46.1

GC content: 69.14

Gene sequence:

>1092_bases
ATGACGAATCGAAACGACGAAACGCAGCAGATGACGGCGATCGTCTGCCACGCCCCCGAGGACTACCGCGTCGAGCGCGT
CGCGAAGCCGCGCGCGAACGCGCGCGAGCTCGTGATCCGCATCGGCGCGTGCGGCATCTGCGCGAGCGACTGCAAGTGCC
ACGCCGGCGCGAAGATGTTCTGGGGCGGGCCGAGCCCGTGGGTCAAGGCGCCCGTGATTCCCGGCCACGAGTTCTTCGGC
TACGTCGAGGCGCTGGGCGAGGGCGCGGCCGAGCACTTCGGCGTCGCGCTCGGCGATCGCGTGATCGCCGAGCAGATCGT
GCCGTGCGGCACGTGCCGCTATTGCAAGTCGGGCCAGTACTGGATGTGCGAGGTCCATCACATCTTCGGCTTTCAGCGCG
AGGTCGCCGACGGCGGGATGGCCGAGTACATGCGCATACCGTCGGGCGCGATCGTCCACCCGGTCCCGCTCGGCATCTCG
CTCGAGGACGCGGCGATCATCGAGCCGCTCGCGTGCGCGATCCACACGGTCAACCGCGGCGACGTCCAGCTCGACGACGT
CGTCGTGATCGCGGGCGCGGGCCCGCTCGGCCTGATGATGACGCAGGTCGCGAAGCTGAAGACGCCCAGGCGGCTCGTCG
TCGTCGATCCCGTCGAAGCGCGGCGCGCGCTCGCGCGCGAATACGGCGCCGACGTGACGATCGATCCGGCCCGCGAGGAC
GCGCCCGCGATCGTGCGCGCGCTGACGGGGGGCTACGGCTGCGACGTCTACATCGAGACGACCGGCGTGCCGGCGGGCGT
CACGCAGGGCATGGCGCTGATCCGCAAGCTCGGCCGCTTCGTCGAGTTCTCGGTGTTCGGCAAGGATACGGCGCTCGACT
GGTCGATCATCGGCGATCGCAAGGAGCTCGACGTACGCGGCGCGCATCTCGGCCCGTATTGCTATCCTGTCGCGATCGAT
CTGCTCGCGCGCGGGCTCGTCACGTCGAAGGGCATCGTCACGCACGGCTTCTCGCTCGACGAATGGGACGAGGCGATCCG
GGTCGCGAACTCGCTCGACTCGATCAAGGTGCTGATGAAGCCGCGCGGCTGA

Upstream 100 bases:

>100_bases
CCCGCCGCGCGCGCTCGATCCGCTTCATCCGCTTCATCCGCGCGCGGGCCGCCGCATCCGGCCTGCCGCTTTCTCGAACC
CAGCCAGCCAAACCGAAACC

Downstream 100 bases:

>100_bases
GCGCGCGGCTCACCGGGACGCGGAGGCGATATGGCATGCGTGATTGGTGTCGACATCGGCACCCAGAGCACGAAGGCGGT
GCTCGTCGGCCTCGACGGCG

Product: oxidoreductase, zinc-binding dehydrogenase family

Products: NA

Alternate protein names: Glucitol dehydrogenase; L-iditol 2-dehydrogenase [H]

Number of amino acids: Translated: 363; Mature: 362

Protein sequence:

>363_residues
MTNRNDETQQMTAIVCHAPEDYRVERVAKPRANARELVIRIGACGICASDCKCHAGAKMFWGGPSPWVKAPVIPGHEFFG
YVEALGEGAAEHFGVALGDRVIAEQIVPCGTCRYCKSGQYWMCEVHHIFGFQREVADGGMAEYMRIPSGAIVHPVPLGIS
LEDAAIIEPLACAIHTVNRGDVQLDDVVVIAGAGPLGLMMTQVAKLKTPRRLVVVDPVEARRALAREYGADVTIDPARED
APAIVRALTGGYGCDVYIETTGVPAGVTQGMALIRKLGRFVEFSVFGKDTALDWSIIGDRKELDVRGAHLGPYCYPVAID
LLARGLVTSKGIVTHGFSLDEWDEAIRVANSLDSIKVLMKPRG

Sequences:

>Translated_363_residues
MTNRNDETQQMTAIVCHAPEDYRVERVAKPRANARELVIRIGACGICASDCKCHAGAKMFWGGPSPWVKAPVIPGHEFFG
YVEALGEGAAEHFGVALGDRVIAEQIVPCGTCRYCKSGQYWMCEVHHIFGFQREVADGGMAEYMRIPSGAIVHPVPLGIS
LEDAAIIEPLACAIHTVNRGDVQLDDVVVIAGAGPLGLMMTQVAKLKTPRRLVVVDPVEARRALAREYGADVTIDPARED
APAIVRALTGGYGCDVYIETTGVPAGVTQGMALIRKLGRFVEFSVFGKDTALDWSIIGDRKELDVRGAHLGPYCYPVAID
LLARGLVTSKGIVTHGFSLDEWDEAIRVANSLDSIKVLMKPRG
>Mature_362_residues
TNRNDETQQMTAIVCHAPEDYRVERVAKPRANARELVIRIGACGICASDCKCHAGAKMFWGGPSPWVKAPVIPGHEFFGY
VEALGEGAAEHFGVALGDRVIAEQIVPCGTCRYCKSGQYWMCEVHHIFGFQREVADGGMAEYMRIPSGAIVHPVPLGISL
EDAAIIEPLACAIHTVNRGDVQLDDVVVIAGAGPLGLMMTQVAKLKTPRRLVVVDPVEARRALAREYGADVTIDPAREDA
PAIVRALTGGYGCDVYIETTGVPAGVTQGMALIRKLGRFVEFSVFGKDTALDWSIIGDRKELDVRGAHLGPYCYPVAIDL
LARGLVTSKGIVTHGFSLDEWDEAIRVANSLDSIKVLMKPRG

Specific function: Reduces glucitol to fructose [H]

COG id: COG1063

COG function: function code ER; Threonine dehydrogenase and related Zn-dependent dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI156627571, Length=350, Percent_Identity=30.8571428571429, Blast_Score=150, Evalue=1e-36,
Organism=Homo sapiens, GI4501929, Length=378, Percent_Identity=24.0740740740741, Blast_Score=69, Evalue=6e-12,
Organism=Homo sapiens, GI71565154, Length=395, Percent_Identity=23.7974683544304, Blast_Score=68, Evalue=1e-11,
Organism=Escherichia coli, GI1787863, Length=340, Percent_Identity=27.6470588235294, Blast_Score=136, Evalue=2e-33,
Organism=Escherichia coli, GI1788073, Length=351, Percent_Identity=29.6296296296296, Blast_Score=131, Evalue=6e-32,
Organism=Escherichia coli, GI1790045, Length=346, Percent_Identity=30.3468208092486, Blast_Score=131, Evalue=7e-32,
Organism=Escherichia coli, GI226510992, Length=348, Percent_Identity=29.0229885057471, Blast_Score=121, Evalue=9e-29,
Organism=Escherichia coli, GI1788407, Length=336, Percent_Identity=27.9761904761905, Blast_Score=118, Evalue=6e-28,
Organism=Escherichia coli, GI1788075, Length=364, Percent_Identity=28.2967032967033, Blast_Score=115, Evalue=4e-27,
Organism=Escherichia coli, GI87082125, Length=284, Percent_Identity=30.9859154929577, Blast_Score=101, Evalue=6e-23,
Organism=Escherichia coli, GI1790718, Length=320, Percent_Identity=26.875, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1786825, Length=279, Percent_Identity=29.7491039426523, Blast_Score=99, Evalue=4e-22,
Organism=Escherichia coli, GI87081918, Length=222, Percent_Identity=31.981981981982, Blast_Score=67, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17562876, Length=359, Percent_Identity=30.3621169916435, Blast_Score=158, Evalue=4e-39,
Organism=Caenorhabditis elegans, GI17562878, Length=357, Percent_Identity=28.8515406162465, Blast_Score=145, Evalue=4e-35,
Organism=Caenorhabditis elegans, GI71997431, Length=261, Percent_Identity=26.0536398467433, Blast_Score=66, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI25146526, Length=261, Percent_Identity=26.4367816091954, Blast_Score=65, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6323099, Length=366, Percent_Identity=27.5956284153005, Blast_Score=113, Evalue=4e-26,
Organism=Saccharomyces cerevisiae, GI6322619, Length=342, Percent_Identity=27.7777777777778, Blast_Score=105, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6319955, Length=342, Percent_Identity=27.7777777777778, Blast_Score=105, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6319257, Length=277, Percent_Identity=28.158844765343, Blast_Score=86, Evalue=7e-18,
Organism=Saccharomyces cerevisiae, GI6319258, Length=274, Percent_Identity=25.9124087591241, Blast_Score=77, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6324486, Length=346, Percent_Identity=28.0346820809249, Blast_Score=73, Evalue=8e-14,
Organism=Saccharomyces cerevisiae, GI6320033, Length=260, Percent_Identity=24.2307692307692, Blast_Score=70, Evalue=7e-13,
Organism=Saccharomyces cerevisiae, GI6323961, Length=323, Percent_Identity=28.4829721362229, Blast_Score=64, Evalue=4e-11,
Organism=Drosophila melanogaster, GI17137530, Length=346, Percent_Identity=29.4797687861272, Blast_Score=122, Evalue=5e-28,
Organism=Drosophila melanogaster, GI17737897, Length=365, Percent_Identity=29.5890410958904, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI221457811, Length=352, Percent_Identity=24.4318181818182, Blast_Score=73, Evalue=3e-13,
Organism=Drosophila melanogaster, GI45550770, Length=352, Percent_Identity=24.4318181818182, Blast_Score=73, Evalue=3e-13,
Organism=Drosophila melanogaster, GI45551930, Length=352, Percent_Identity=24.4318181818182, Blast_Score=73, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR002328
- InterPro:   IPR011032
- InterPro:   IPR016040 [H]

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N [H]

EC number: =1.1.1.14 [H]

Molecular weight: Translated: 39225; Mature: 39094

Theoretical pI: Translated: 6.34; Mature: 6.34

Prosite motif: PS00059 ADH_ZINC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
3.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNRNDETQQMTAIVCHAPEDYRVERVAKPRANARELVIRIGACGICASDCKCHAGAKMF
CCCCCCCHHEEEEEEEECCCCCCHHHHHCCCCCCEEEEEEECCCCEECCCCCCCCCCEEE
WGGPSPWVKAPVIPGHEFFGYVEALGEGAAEHFGVALGDRVIAEQIVPCGTCRYCKSGQY
ECCCCCCEECCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCE
WMCEVHHIFGFQREVADGGMAEYMRIPSGAIVHPVPLGISLEDAAIIEPLACAIHTVNRG
EEEEEHHHHCHHHHHHCCCHHHHHCCCCCCEEECCCCCCEECCHHHHHHHHHHHEECCCC
DVQLDDVVVIAGAGPLGLMMTQVAKLKTPRRLVVVDPVEARRALAREYGADVTIDPARED
CEEECCEEEEECCCHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHCCCEEECCCCCC
APAIVRALTGGYGCDVYIETTGVPAGVTQGMALIRKLGRFVEFSVFGKDTALDWSIIGDR
HHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHEEEEEECCCCCEEEEEECCC
KELDVRGAHLGPYCYPVAIDLLARGLVTSKGIVTHGFSLDEWDEAIRVANSLDSIKVLMK
CCCEECCCCCCCHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHCCCCCEEEEEC
PRG
CCC
>Mature Secondary Structure 
TNRNDETQQMTAIVCHAPEDYRVERVAKPRANARELVIRIGACGICASDCKCHAGAKMF
CCCCCCHHEEEEEEEECCCCCCHHHHHCCCCCCEEEEEEECCCCEECCCCCCCCCCEEE
WGGPSPWVKAPVIPGHEFFGYVEALGEGAAEHFGVALGDRVIAEQIVPCGTCRYCKSGQY
ECCCCCCEECCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCE
WMCEVHHIFGFQREVADGGMAEYMRIPSGAIVHPVPLGISLEDAAIIEPLACAIHTVNRG
EEEEEHHHHCHHHHHHCCCHHHHHCCCCCCEEECCCCCCEECCHHHHHHHHHHHEECCCC
DVQLDDVVVIAGAGPLGLMMTQVAKLKTPRRLVVVDPVEARRALAREYGADVTIDPARED
CEEECCEEEEECCCHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHCCCEEECCCCCC
APAIVRALTGGYGCDVYIETTGVPAGVTQGMALIRKLGRFVEFSVFGKDTALDWSIIGDR
HHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHEEEEEECCCCCEEEEEECCC
KELDVRGAHLGPYCYPVAIDLLARGLVTSKGIVTHGFSLDEWDEAIRVANSLDSIKVLMK
CCCEECCCCCCCHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHCCCCCEEEEEC
PRG
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10086842; 11058132 [H]