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The map label for this gene is cysW [H]

Identifier: 237812140

GI number: 237812140

Start: 1825700

End: 1826734

Strand: Direct

Name: cysW [H]

Synonym: GBP346_A1886

Alternate gene names: 237812140

Gene position: 1825700-1826734 (Clockwise)

Preceding gene: 237812139

Following gene: 237812141

Centisome position: 44.54

GC content: 70.05

Gene sequence:

>1035_bases
ATGAGCCGCGATACCGCTCGATCCCGCGCGGGCGCCGCGCGCGCCGACGCTGGCCCGGAATTCGGCGCTCGATTCGGCCT
TGAATCCGGCCTTGAATCCGGCGATGACGCGCGCCGCGCCGCGCGCGCCCCGGCGCGAGCGCGCCGGCTCGACCCCGTGA
GCGAGCCGCGCGCGGTGCGCTGGCTGCTCACGGGCGCGGCGCTCGCGTTCCTCGCGCTCTTTCTCGTCGTGCCGCTTGCC
GCGGTGTTCTTCGAGGCGCTGCGCAAGGGCGTCGATTTCTATCTCGAATCGCTCGCCGATCCGGACGCGTGGTCGGCGAT
CAAGCTCACGCTCGTCGTCGCCGCGATCGCCGTGCCGCTCAATCTCGTATTCGGCGTGTGCGCGTCGTGGGCGATCGCGA
AGTTCGAGTTCAAGGGCAAGGCGGTGCTGACGACGCTCATCGATCTGCCGTTCTCGGTGTCGCCCGTGATCTCGGGCCTC
GTCTACGTGCTGCTGTTCGGCGCGCAGGGCTGGCTCGGCCCGTGGCTGCAGGCGCATGACGTGCAGATCATCTTCGCCGT
GCCGGGCATCGTGCTCGCGACGATCTTCGTCACGTTCCCGTTCGTCGCGCGCGAGCTGATTCCGCTGATGCAGGCGCAGG
GCGCCGACGAGGAGGAGGCCGCGCGCGTGCTCGGCGCATCCGGCTGGCAGATCTTCCGGCGCGTCACGCTGCCGAACGTG
AAGTGGGGCCTGCTGTACGGCGTGATTCTCTGCAACGCGCGCGCAATGGGCGAGTTCGGCGCGGTGTCGGTGGTGTCGGG
CCATATCCGCGGGCAGACCGACACGATGCCGCTGCACGTCGAGATTCTCTACAACGAGTACAACTTCGCGGCCGCGTTCG
CGGTGGCGTCGGTACTCGCGCTGCTCGCGCTCGTCACGCTCGCGCTCAAGCTGTTCGCCGAGCGGCGGCTGTCCGCCGAA
CTCGCGCACGGGCGCGACGACGCGAGCGCCCCCGCCGCGCACCCGGGCGCCGCCGTCACTTCGTCGATTTCGTAA

Upstream 100 bases:

>100_bases
TCATCAACACGCTGCAGTGGTATCTGCAGCGCCGCACGAGCCGCGGCGCGAGCGGCCCGACGCCGGCCGCGCATGCGGCG
ACGGCCCTCGGAGGCGCGCA

Downstream 100 bases:

>100_bases
AGAGGCAAACCAGATGGGCATCACCGTTCGTAATCTGCACAAACGCTTCGGCGAGTTCGCCGCGCTCGACGACGTCTCGC
TCGATTTCCCGGCGGGTGAG

Product: sulfate ABC transporter, permease protein CysW

Products: ADP; phosphate; S2O32- [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 344; Mature: 343

Protein sequence:

>344_residues
MSRDTARSRAGAARADAGPEFGARFGLESGLESGDDARRAARAPARARRLDPVSEPRAVRWLLTGAALAFLALFLVVPLA
AVFFEALRKGVDFYLESLADPDAWSAIKLTLVVAAIAVPLNLVFGVCASWAIAKFEFKGKAVLTTLIDLPFSVSPVISGL
VYVLLFGAQGWLGPWLQAHDVQIIFAVPGIVLATIFVTFPFVARELIPLMQAQGADEEEAARVLGASGWQIFRRVTLPNV
KWGLLYGVILCNARAMGEFGAVSVVSGHIRGQTDTMPLHVEILYNEYNFAAAFAVASVLALLALVTLALKLFAERRLSAE
LAHGRDDASAPAAHPGAAVTSSIS

Sequences:

>Translated_344_residues
MSRDTARSRAGAARADAGPEFGARFGLESGLESGDDARRAARAPARARRLDPVSEPRAVRWLLTGAALAFLALFLVVPLA
AVFFEALRKGVDFYLESLADPDAWSAIKLTLVVAAIAVPLNLVFGVCASWAIAKFEFKGKAVLTTLIDLPFSVSPVISGL
VYVLLFGAQGWLGPWLQAHDVQIIFAVPGIVLATIFVTFPFVARELIPLMQAQGADEEEAARVLGASGWQIFRRVTLPNV
KWGLLYGVILCNARAMGEFGAVSVVSGHIRGQTDTMPLHVEILYNEYNFAAAFAVASVLALLALVTLALKLFAERRLSAE
LAHGRDDASAPAAHPGAAVTSSIS
>Mature_343_residues
SRDTARSRAGAARADAGPEFGARFGLESGLESGDDARRAARAPARARRLDPVSEPRAVRWLLTGAALAFLALFLVVPLAA
VFFEALRKGVDFYLESLADPDAWSAIKLTLVVAAIAVPLNLVFGVCASWAIAKFEFKGKAVLTTLIDLPFSVSPVISGLV
YVLLFGAQGWLGPWLQAHDVQIIFAVPGIVLATIFVTFPFVARELIPLMQAQGADEEEAARVLGASGWQIFRRVTLPNVK
WGLLYGVILCNARAMGEFGAVSVVSGHIRGQTDTMPLHVEILYNEYNFAAAFAVASVLALLALVTLALKLFAERRLSAEL
AHGRDDASAPAAHPGAAVTSSIS

Specific function: Part of the ABC transporter complex CysAWTP (TC 3.A.1.6.1) involved in sulfate/thiosulfate import. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG4208

COG function: function code P; ABC-type sulfate transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI87082099, Length=268, Percent_Identity=52.6119402985075, Blast_Score=298, Evalue=3e-82,
Organism=Escherichia coli, GI1788764, Length=206, Percent_Identity=36.8932038834951, Blast_Score=149, Evalue=4e-37,
Organism=Escherichia coli, GI1786980, Length=181, Percent_Identity=34.8066298342541, Blast_Score=95, Evalue=6e-21,
Organism=Escherichia coli, GI1787369, Length=175, Percent_Identity=28, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR011866
- InterPro:   IPR005667 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 36652; Mature: 36521

Theoretical pI: Translated: 7.63; Mature: 7.63

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRDTARSRAGAARADAGPEFGARFGLESGLESGDDARRAARAPARARRLDPVSEPRAVR
CCCCHHHHHCCCCCCCCCCCHHHHHCHHHCCCCCHHHHHHHCCCHHHHCCCCCCCCHHHH
WLLTGAALAFLALFLVVPLAAVFFEALRKGVDFYLESLADPDAWSAIKLTLVVAAIAVPL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
NLVFGVCASWAIAKFEFKGKAVLTTLIDLPFSVSPVISGLVYVLLFGAQGWLGPWLQAHD
HHHHHHHHHHHHHHEECCCHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCC
VQIIFAVPGIVLATIFVTFPFVARELIPLMQAQGADEEEAARVLGASGWQIFRRVTLPNV
EEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHCCCCC
KWGLLYGVILCNARAMGEFGAVSVVSGHIRGQTDTMPLHVEILYNEYNFAAAFAVASVLA
HHHHHHHHHHHCCHHCCCCCCHHHHCCCCCCCCCCCEEEEEEEECCCHHHHHHHHHHHHH
LLALVTLALKLFAERRLSAELAHGRDDASAPAAHPGAAVTSSIS
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SRDTARSRAGAARADAGPEFGARFGLESGLESGDDARRAARAPARARRLDPVSEPRAVR
CCCHHHHHCCCCCCCCCCCHHHHHCHHHCCCCCHHHHHHHCCCHHHHCCCCCCCCHHHH
WLLTGAALAFLALFLVVPLAAVFFEALRKGVDFYLESLADPDAWSAIKLTLVVAAIAVPL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
NLVFGVCASWAIAKFEFKGKAVLTTLIDLPFSVSPVISGLVYVLLFGAQGWLGPWLQAHD
HHHHHHHHHHHHHHEECCCHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCC
VQIIFAVPGIVLATIFVTFPFVARELIPLMQAQGADEEEAARVLGASGWQIFRRVTLPNV
EEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHCCCCC
KWGLLYGVILCNARAMGEFGAVSVVSGHIRGQTDTMPLHVEILYNEYNFAAAFAVASVLA
HHHHHHHHHHHCCHHCCCCCCHHHHCCCCCCCCCCCEEEEEEEECCCHHHHHHHHHHHHH
LLALVTLALKLFAERRLSAELAHGRDDASAPAAHPGAAVTSSIS
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; S2O32- [Periplasm]; H2O; SO42- [Periplasm]; ATP [C]

Specific reaction: ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]