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The map label for this gene is cht60 [H]

Identifier: 237811936

GI number: 237811936

Start: 1613278

End: 1615377

Strand: Direct

Name: cht60 [H]

Synonym: GBP346_A1677

Alternate gene names: 237811936

Gene position: 1613278-1615377 (Clockwise)

Preceding gene: 237811934

Following gene: 237811939

Centisome position: 39.36

GC content: 69.95

Gene sequence:

>2100_bases
ATGAAACGACGTGAATGCCGCGGGAATGCGCGCGGCAAGGATTCGGTGAAGATGCGCAAGAAAAGCTTGGTTCATTTGCC
CGTCGCGCTGGCGGTGGCCGCGGCGCTCGGCGCGTGCTCGGGCGACGACGACGCGACGCTCGAGTCGCGCGCGGACGCGA
TCGTCGAACGCATGACGACCCGGCAGAAGGTCGGCCAGAAACTGATGATGGCGTTCCGGTACTGGTGCCCCGACGGGCAG
CCCGCGTGCACGGCCGGCATGACCGAATTTCCCGACGCCGCGCGCGACGCGCTGCGCGAGAACGGCATTGGCGGCGTGAT
CCTGTTCTCGAACAACCTGACGGGCATCAAGCAGACGCGCCGGCTGATCGACGGCATTCGGGCCGCGCCCGCGGCCGACA
GCCCGCTCGGGCTGATGATCGGCATCGACGAGGAGGGCGGCAACGTGTTTCGCCTGCCGCGCGTCGAGGCGACCGCGTTC
GCCGGCAACATGGCGCTCGGCGCGGCGTACGAAGCGACGCGGGACGACCGGCTCGCGTACGACATGGGCCGCGTGCTCGC
CGCGGAGATCGCGGCGGTCGGCTTCAACGTGAATTTCGCGCCGGACGTCGACGTCAACAGCAATCCGCTCAATCCGGTGA
TCAACGTGCGCGCGTTCGGCGACGATCCGGCGACGATCGGCCTGCTCGGCCGGCGCATGGTGCAGGGCATGAAGAGCGAG
CGCGTGATCGGCACGTTCAAGCATTTTCCCGGGCACGGCGACACGGATACCGATTCGCACTACGGATTGCCCGTCGTGAT
CAAGTCGCGCGCCGACGCCTACGCGATCGATCTCGCGCCGTACCGGCAGGCGATCGAAGCGGGCGAGGCGCCCGACATGA
TCATGACCGCGCACATCCAGTATCCGTCGCTCGACGACACCCGCGTCGCGACCCGCACGGGCGAGCAGATGATCGCGCCC
GCGACGATGTCGCGGCGCATCCAGCACGATATCCTGCGCGGCGAGTTCGGCTATCAGGGCGTGACGATCACCGACGCGCT
CGACATGAAGGGCATCGCCGGCTTCTTCGACGAGGACGACGCGGTCGTCAAGGTGTTCCAGGCGGACGTCGACATTGCGC
TGATGCCCGTCGAGTTTCGCACCGCGGCCGATGCCGGGCGCCTGGCGGCGCTCGTCGATCGCGTCGCCGCGGCGGTCGAT
TCGGGGCGGATCGATCGCGCCGAGTTCGATCGCTCGGTGCGCCGTATCGTGCTGACGAAGCTGCGCCACGGCATCGTCGC
GTCGGATCGCGGCCGGCCGGTCGACGAGCTGGCATCGATCGGCGGGCCCGCGCACCGCGCGATCGAGCGCGACATCGCGC
AAAAATCGATCACCGTGCTGCGCAACGAGAACGGCGCGCTGCCGCTGCAGGCCGCGGGCCGGCGCATCTTCATCCTGACG
CCGTGGGGCGAGCAGGCCGAGGCGATGCGGCGGCGTTTCGTCGAGCTTGGCCATCCGCTCGTCACGGGCGCGAAGCTGAG
CGCGATCACGTGGGCCGAACAGCAGCAGGCGATCGATGCGGCCGACGTCGTGATCGTCGGCACGCTGTCGACGGGCGTGA
CGCCCGTCGAGCACAACGGGGATCCGAACGCGCGCGTGAGCCCGCCGGCGCCGTCGGCCGTGCGGATGCGGCAGGCCGCG
CCCGCGAACGGCGAGGAGGAGGGTTCGGTGATCTTCGACCACGTCGAGCGGGCGGACGCGGCGAAGGACATCGGCGCGCG
CCCGAGCGTGCTCGCGGCGATCGCGGCGCCGAGCGAGGCGCAGCAGATGCGCGACGCGATGGACTACGCGAAGGCGCGGC
GCAAGACCGTGATCCACGTGACGATGCGCGCGCCGTACGACGTGATCAGCTACGACGATGTCGCGGACGCGACGCTCGCC
ACCTACGCGTACTACGGCTACGAAGGCGGCTTGCGCGGCCCGTCGCTGCCCGCGGCCGTCGACGCGATGCTCGGCGTCGC
GCGGCCGGTCGGCAGGCTGCCGGTCGCGATCCACGCACTGAACGCGGACGGATCGACGGGGCCGCTGCGCTACGCGCGCG
GATTCGGGTTGCAGTACTGA

Upstream 100 bases:

>100_bases
CATTTGACGAATTCGATGAATGGGCATTCTGAGTGAATGAATACGCGTGAAGTTCCGGAGTACCGATGAACCGAAAGCCG
ATAGGGATGCCGGATGACGA

Downstream 100 bases:

>100_bases
GGCCGGACGCCGGCGACGCCGCGTCGCTGTCGGCGCGGCGTCGAGCGACACGCCGCGCGCGCAGGCGGGCTGCGGCGGCG
CGGCCGATCGCCGCGCGTTA

Product: glycosyl hydrolase, family 3

Products: NA

Alternate protein names: Beta-N-acetylhexosaminidase; Chitobiase; N-acetyl-beta-glucosaminidase [H]

Number of amino acids: Translated: 699; Mature: 699

Protein sequence:

>699_residues
MKRRECRGNARGKDSVKMRKKSLVHLPVALAVAAALGACSGDDDATLESRADAIVERMTTRQKVGQKLMMAFRYWCPDGQ
PACTAGMTEFPDAARDALRENGIGGVILFSNNLTGIKQTRRLIDGIRAAPAADSPLGLMIGIDEEGGNVFRLPRVEATAF
AGNMALGAAYEATRDDRLAYDMGRVLAAEIAAVGFNVNFAPDVDVNSNPLNPVINVRAFGDDPATIGLLGRRMVQGMKSE
RVIGTFKHFPGHGDTDTDSHYGLPVVIKSRADAYAIDLAPYRQAIEAGEAPDMIMTAHIQYPSLDDTRVATRTGEQMIAP
ATMSRRIQHDILRGEFGYQGVTITDALDMKGIAGFFDEDDAVVKVFQADVDIALMPVEFRTAADAGRLAALVDRVAAAVD
SGRIDRAEFDRSVRRIVLTKLRHGIVASDRGRPVDELASIGGPAHRAIERDIAQKSITVLRNENGALPLQAAGRRIFILT
PWGEQAEAMRRRFVELGHPLVTGAKLSAITWAEQQQAIDAADVVIVGTLSTGVTPVEHNGDPNARVSPPAPSAVRMRQAA
PANGEEEGSVIFDHVERADAAKDIGARPSVLAAIAAPSEAQQMRDAMDYAKARRKTVIHVTMRAPYDVISYDDVADATLA
TYAYYGYEGGLRGPSLPAAVDAMLGVARPVGRLPVAIHALNADGSTGPLRYARGFGLQY

Sequences:

>Translated_699_residues
MKRRECRGNARGKDSVKMRKKSLVHLPVALAVAAALGACSGDDDATLESRADAIVERMTTRQKVGQKLMMAFRYWCPDGQ
PACTAGMTEFPDAARDALRENGIGGVILFSNNLTGIKQTRRLIDGIRAAPAADSPLGLMIGIDEEGGNVFRLPRVEATAF
AGNMALGAAYEATRDDRLAYDMGRVLAAEIAAVGFNVNFAPDVDVNSNPLNPVINVRAFGDDPATIGLLGRRMVQGMKSE
RVIGTFKHFPGHGDTDTDSHYGLPVVIKSRADAYAIDLAPYRQAIEAGEAPDMIMTAHIQYPSLDDTRVATRTGEQMIAP
ATMSRRIQHDILRGEFGYQGVTITDALDMKGIAGFFDEDDAVVKVFQADVDIALMPVEFRTAADAGRLAALVDRVAAAVD
SGRIDRAEFDRSVRRIVLTKLRHGIVASDRGRPVDELASIGGPAHRAIERDIAQKSITVLRNENGALPLQAAGRRIFILT
PWGEQAEAMRRRFVELGHPLVTGAKLSAITWAEQQQAIDAADVVIVGTLSTGVTPVEHNGDPNARVSPPAPSAVRMRQAA
PANGEEEGSVIFDHVERADAAKDIGARPSVLAAIAAPSEAQQMRDAMDYAKARRKTVIHVTMRAPYDVISYDDVADATLA
TYAYYGYEGGLRGPSLPAAVDAMLGVARPVGRLPVAIHALNADGSTGPLRYARGFGLQY
>Mature_699_residues
MKRRECRGNARGKDSVKMRKKSLVHLPVALAVAAALGACSGDDDATLESRADAIVERMTTRQKVGQKLMMAFRYWCPDGQ
PACTAGMTEFPDAARDALRENGIGGVILFSNNLTGIKQTRRLIDGIRAAPAADSPLGLMIGIDEEGGNVFRLPRVEATAF
AGNMALGAAYEATRDDRLAYDMGRVLAAEIAAVGFNVNFAPDVDVNSNPLNPVINVRAFGDDPATIGLLGRRMVQGMKSE
RVIGTFKHFPGHGDTDTDSHYGLPVVIKSRADAYAIDLAPYRQAIEAGEAPDMIMTAHIQYPSLDDTRVATRTGEQMIAP
ATMSRRIQHDILRGEFGYQGVTITDALDMKGIAGFFDEDDAVVKVFQADVDIALMPVEFRTAADAGRLAALVDRVAAAVD
SGRIDRAEFDRSVRRIVLTKLRHGIVASDRGRPVDELASIGGPAHRAIERDIAQKSITVLRNENGALPLQAAGRRIFILT
PWGEQAEAMRRRFVELGHPLVTGAKLSAITWAEQQQAIDAADVVIVGTLSTGVTPVEHNGDPNARVSPPAPSAVRMRQAA
PANGEEEGSVIFDHVERADAAKDIGARPSVLAAIAAPSEAQQMRDAMDYAKARRKTVIHVTMRAPYDVISYDDVADATLA
TYAYYGYEGGLRGPSLPAAVDAMLGVARPVGRLPVAIHALNADGSTGPLRYARGFGLQY

Specific function: Most active towards p-nitrophenyl-N-acetyl-beta-D- glucosaminide(PNP-beta-GlcNAc) and diacetylchitobiose [H]

COG id: COG1472

COG function: function code G; Beta-glucosidase-related glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 3 family [H]

Homologues:

Organism=Escherichia coli, GI1787350, Length=259, Percent_Identity=31.2741312741313, Blast_Score=109, Evalue=6e-25,
Organism=Escherichia coli, GI1788453, Length=686, Percent_Identity=23.1778425655977, Blast_Score=91, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019800
- InterPro:   IPR001764
- InterPro:   IPR017853 [H]

Pfam domain/function: PF00933 Glyco_hydro_3 [H]

EC number: =3.2.1.52 [H]

Molecular weight: Translated: 75039; Mature: 75039

Theoretical pI: Translated: 6.59; Mature: 6.59

Prosite motif: PS00775 GLYCOSYL_HYDROL_F3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRRECRGNARGKDSVKMRKKSLVHLPVALAVAAALGACSGDDDATLESRADAIVERMTT
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
RQKVGQKLMMAFRYWCPDGQPACTAGMTEFPDAARDALRENGIGGVILFSNNLTGIKQTR
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEECCCCCHHHHHH
RLIDGIRAAPAADSPLGLMIGIDEEGGNVFRLPRVEATAFAGNMALGAAYEATRDDRLAY
HHHHHHHCCCCCCCCCEEEEEEECCCCCEEEECCCCHHHCCCCCCCCCHHHCCCCCHHHH
DMGRVLAAEIAAVGFNVNFAPDVDVNSNPLNPVINVRAFGDDPATIGLLGRRMVQGMKSE
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHCCCCC
RVIGTFKHFPGHGDTDTDSHYGLPVVIKSRADAYAIDLAPYRQAIEAGEAPDMIMTAHIQ
CEEEHHHCCCCCCCCCCCCCCCCCEEEECCCCEEEEECHHHHHHHHCCCCCCEEEEEEEE
YPSLDDTRVATRTGEQMIAPATMSRRIQHDILRGEFGYQGVTITDALDMKGIAGFFDEDD
CCCCCCCHHHHHCCCHHCCCHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCCCCCC
AVVKVFQADVDIALMPVEFRTAADAGRLAALVDRVAAAVDSGRIDRAEFDRSVRRIVLTK
CEEEEEECCCCEEEEEEEHHCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
LRHGIVASDRGRPVDELASIGGPAHRAIERDIAQKSITVLRNENGALPLQAAGRRIFILT
HHHCCEECCCCCCHHHHHHCCCHHHHHHHHHHHHHHEEEEECCCCCCCHHHCCCEEEEEC
PWGEQAEAMRRRFVELGHPLVTGAKLSAITWAEQQQAIDAADVVIVGTLSTGVTPVEHNG
CCCHHHHHHHHHHHHHCCCEECCCEEEEEEHHHHHHCCCCCCEEEEEECCCCCCEECCCC
DPNARVSPPAPSAVRMRQAAPANGEEEGSVIFDHVERADAAKDIGARPSVLAAIAAPSEA
CCCCEECCCCCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCHHH
QQMRDAMDYAKARRKTVIHVTMRAPYDVISYDDVADATLATYAYYGYEGGLRGPSLPAAV
HHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCHHHHHHHHHEEECCCCCCCCCCCHHHH
DAMLGVARPVGRLPVAIHALNADGSTGPLRYARGFGLQY
HHHHHHHCCCCCCCEEEEEECCCCCCCCHHHHCCCCCCC
>Mature Secondary Structure
MKRRECRGNARGKDSVKMRKKSLVHLPVALAVAAALGACSGDDDATLESRADAIVERMTT
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
RQKVGQKLMMAFRYWCPDGQPACTAGMTEFPDAARDALRENGIGGVILFSNNLTGIKQTR
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEECCCCCHHHHHH
RLIDGIRAAPAADSPLGLMIGIDEEGGNVFRLPRVEATAFAGNMALGAAYEATRDDRLAY
HHHHHHHCCCCCCCCCEEEEEEECCCCCEEEECCCCHHHCCCCCCCCCHHHCCCCCHHHH
DMGRVLAAEIAAVGFNVNFAPDVDVNSNPLNPVINVRAFGDDPATIGLLGRRMVQGMKSE
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHCCCCC
RVIGTFKHFPGHGDTDTDSHYGLPVVIKSRADAYAIDLAPYRQAIEAGEAPDMIMTAHIQ
CEEEHHHCCCCCCCCCCCCCCCCCEEEECCCCEEEEECHHHHHHHHCCCCCCEEEEEEEE
YPSLDDTRVATRTGEQMIAPATMSRRIQHDILRGEFGYQGVTITDALDMKGIAGFFDEDD
CCCCCCCHHHHHCCCHHCCCHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCCCCCC
AVVKVFQADVDIALMPVEFRTAADAGRLAALVDRVAAAVDSGRIDRAEFDRSVRRIVLTK
CEEEEEECCCCEEEEEEEHHCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
LRHGIVASDRGRPVDELASIGGPAHRAIERDIAQKSITVLRNENGALPLQAAGRRIFILT
HHHCCEECCCCCCHHHHHHCCCHHHHHHHHHHHHHHEEEEECCCCCCCHHHCCCEEEEEC
PWGEQAEAMRRRFVELGHPLVTGAKLSAITWAEQQQAIDAADVVIVGTLSTGVTPVEHNG
CCCHHHHHHHHHHHHHCCCEECCCEEEEEEHHHHHHCCCCCCEEEEEECCCCCCEECCCC
DPNARVSPPAPSAVRMRQAAPANGEEEGSVIFDHVERADAAKDIGARPSVLAAIAAPSEA
CCCCEECCCCCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCHHH
QQMRDAMDYAKARRKTVIHVTMRAPYDVISYDDVADATLATYAYYGYEGGLRGPSLPAAV
HHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCHHHHHHHHHEEECCCCCCCCCCCHHHH
DAMLGVARPVGRLPVAIHALNADGSTGPLRYARGFGLQY
HHHHHHHCCCCCCCEEEEEECCCCCCCCHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8063094 [H]