BacMap

Definition	Brucella suis 1330 chromosome chromosome I, complete sequence.
Accession	NC_004310
Length	2,107,794

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The map label for this gene is paaF [C]

Identifier: 23502894

GI number: 23502894

Start: 1970822

End: 1971598

Strand: Reverse

Name: paaF [C]

Synonym: BR2046

Alternate gene names: 23502894

Gene position: 1971598-1970822 (Counterclockwise)

Preceding gene: 23502895

Following gene: 23502893

Centisome position: 93.54

GC content: 60.88

Gene sequence:

>777_bases
ATGGCTGACAGAATAGAAATTTCGATCGAAAACCACATCGCGGTCATGACAATCAGGCGGCCGGAAAAGCTCAATGCGCT
CGATATACCGCTCTTGCAGGAACTCTCTACCGCCTGTGATCAGGTCGAGGCGGACAGTAGCGTGCGGGTCGCAATCCTGA
CCGGTGAGGGCAAGGCTTTTTCCGCAGGTGGTGACATCAAGGCATGGGGTGGCATGGAGCCGCAAGAATTCGGCCATGCA
TGGGTGCGCTATGGGCATCGTGTGTTCGAGCGGCTGGCGACGTTGCGCGTGCCATTGATCGCGGCCATGAACGGCCATGC
GCTGGGGGGCGGTCTGGAACTGGCGGGAACCGCCGATATCCGCATTGTTGAGCGACAGGCGAAGATCGGCCTGCCGGAAA
CTTCGCTTGGCATGATCCCCGGCTGGTCGGGCACGCAGCGCCTTGTGCGCCGTTTCGGGGCGCAGATCGTGCGCCGCATG
GTGCTGGGCGGCGAAATGTTTACGGCAGATGAAGCCCTGTCGCATGGGCTGGTCGATCATGTGGTCGAGACGGGCGAGGC
TTTGCAGGCCGCGCTGGCCTATGCGGCGCGGGTGGCCAAACGCGGCCCCGCCGCGCTCGAAATTTCCAAACTGATGCTGT
CCGTCGCCAATGGCGAGGACAATGGAACCGCCGTTGAGGCGCTTGGCTCCATTCTTGCCGCCAAGACGGGCGATCTGAAA
GAAGGGGTGCGCTCGTTTACCGAAAAGCGCGAAGCGAATTTTGAAGGAAAATGGTAA

Upstream 100 bases:

>100_bases
GTCTCGGATCAATTGAAGCTCATGGATGAGGCGCTGTTCCGCGAAGAGCCGATCGGGCTTACGCTGCCGCAAAAGCCTGC
GCGAAAGCTGGAGGCGTGAC

Downstream 100 bases:

>100_bases
TGAGCACGATCGTTAAGCCACAGCCGTTGACCGGGCTGGAAGTTCGCGAATTCTCCATGCTGATCGATGGAAAATGGGTG
GGCAGCGACAGCGGAAAGAG

Product: enoyl-CoA hydratase/isomerase family protein

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 258; Mature: 257

Protein sequence:

>258_residues
MADRIEISIENHIAVMTIRRPEKLNALDIPLLQELSTACDQVEADSSVRVAILTGEGKAFSAGGDIKAWGGMEPQEFGHA
WVRYGHRVFERLATLRVPLIAAMNGHALGGGLELAGTADIRIVERQAKIGLPETSLGMIPGWSGTQRLVRRFGAQIVRRM
VLGGEMFTADEALSHGLVDHVVETGEALQAALAYAARVAKRGPAALEISKLMLSVANGEDNGTAVEALGSILAAKTGDLK
EGVRSFTEKREANFEGKW

Sequences:

>Translated_258_residues
MADRIEISIENHIAVMTIRRPEKLNALDIPLLQELSTACDQVEADSSVRVAILTGEGKAFSAGGDIKAWGGMEPQEFGHA
WVRYGHRVFERLATLRVPLIAAMNGHALGGGLELAGTADIRIVERQAKIGLPETSLGMIPGWSGTQRLVRRFGAQIVRRM
VLGGEMFTADEALSHGLVDHVVETGEALQAALAYAARVAKRGPAALEISKLMLSVANGEDNGTAVEALGSILAAKTGDLK
EGVRSFTEKREANFEGKW
>Mature_257_residues
ADRIEISIENHIAVMTIRRPEKLNALDIPLLQELSTACDQVEADSSVRVAILTGEGKAFSAGGDIKAWGGMEPQEFGHAW
VRYGHRVFERLATLRVPLIAAMNGHALGGGLELAGTADIRIVERQAKIGLPETSLGMIPGWSGTQRLVRRFGAQIVRRMV
LGGEMFTADEALSHGLVDHVVETGEALQAALAYAARVAKRGPAALEISKLMLSVANGEDNGTAVEALGSILAAKTGDLKE
GVRSFTEKREANFEGKW

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=256, Percent_Identity=35.15625, Blast_Score=135, Evalue=4e-32,
Organism=Homo sapiens, GI4502327, Length=256, Percent_Identity=36.71875, Blast_Score=114, Evalue=6e-26,
Organism=Homo sapiens, GI68989263, Length=193, Percent_Identity=33.6787564766839, Blast_Score=106, Evalue=2e-23,
Organism=Homo sapiens, GI20127408, Length=183, Percent_Identity=34.4262295081967, Blast_Score=97, Evalue=1e-20,
Organism=Homo sapiens, GI70995211, Length=259, Percent_Identity=28.1853281853282, Blast_Score=90, Evalue=2e-18,
Organism=Homo sapiens, GI31542718, Length=240, Percent_Identity=25.4166666666667, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI157694516, Length=216, Percent_Identity=27.3148148148148, Blast_Score=85, Evalue=5e-17,
Organism=Homo sapiens, GI213417737, Length=216, Percent_Identity=27.3148148148148, Blast_Score=85, Evalue=6e-17,
Organism=Homo sapiens, GI62530384, Length=208, Percent_Identity=31.25, Blast_Score=82, Evalue=6e-16,
Organism=Homo sapiens, GI150378541, Length=256, Percent_Identity=29.6875, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI157694520, Length=174, Percent_Identity=29.3103448275862, Blast_Score=72, Evalue=4e-13,
Organism=Homo sapiens, GI45643119, Length=218, Percent_Identity=25.2293577981651, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI260275230, Length=218, Percent_Identity=25.2293577981651, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI260274832, Length=218, Percent_Identity=25.2293577981651, Blast_Score=72, Evalue=7e-13,
Organism=Homo sapiens, GI261878539, Length=95, Percent_Identity=41.0526315789474, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI37594469, Length=173, Percent_Identity=26.5895953757225, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI37594471, Length=173, Percent_Identity=26.5895953757225, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI295842266, Length=206, Percent_Identity=27.6699029126214, Blast_Score=66, Evalue=4e-11,
Organism=Escherichia coli, GI1787659, Length=253, Percent_Identity=35.1778656126482, Blast_Score=151, Evalue=5e-38,
Organism=Escherichia coli, GI1790281, Length=263, Percent_Identity=31.9391634980989, Blast_Score=115, Evalue=4e-27,
Organism=Escherichia coli, GI221142681, Length=268, Percent_Identity=33.2089552238806, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1788682, Length=191, Percent_Identity=35.6020942408377, Blast_Score=104, Evalue=6e-24,
Organism=Escherichia coli, GI1787660, Length=263, Percent_Identity=31.5589353612167, Blast_Score=97, Evalue=7e-22,
Organism=Escherichia coli, GI87082183, Length=255, Percent_Identity=27.843137254902, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI1788597, Length=257, Percent_Identity=28.0155642023346, Blast_Score=81, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI25145438, Length=241, Percent_Identity=36.5145228215768, Blast_Score=133, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI17554946, Length=250, Percent_Identity=33.2, Blast_Score=128, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI17540714, Length=214, Percent_Identity=33.6448598130841, Blast_Score=122, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17558304, Length=175, Percent_Identity=32, Blast_Score=97, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17534483, Length=220, Percent_Identity=25.9090909090909, Blast_Score=91, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17508951, Length=218, Percent_Identity=30.2752293577982, Blast_Score=90, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI25144276, Length=218, Percent_Identity=32.1100917431193, Blast_Score=90, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17508953, Length=218, Percent_Identity=32.1100917431193, Blast_Score=90, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17560910, Length=187, Percent_Identity=31.5508021390374, Blast_Score=88, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17540306, Length=247, Percent_Identity=26.3157894736842, Blast_Score=81, Evalue=7e-16,
Organism=Caenorhabditis elegans, GI17549921, Length=215, Percent_Identity=26.5116279069767, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI25144157, Length=197, Percent_Identity=30.4568527918782, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI25144160, Length=197, Percent_Identity=30.4568527918782, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI25141363, Length=197, Percent_Identity=25.8883248730964, Blast_Score=74, Evalue=7e-14,
Organism=Saccharomyces cerevisiae, GI6320241, Length=203, Percent_Identity=26.1083743842365, Blast_Score=72, Evalue=8e-14,
Organism=Drosophila melanogaster, GI20129971, Length=250, Percent_Identity=36.8, Blast_Score=136, Evalue=2e-32,
Organism=Drosophila melanogaster, GI24653477, Length=250, Percent_Identity=36.8, Blast_Score=136, Evalue=2e-32,
Organism=Drosophila melanogaster, GI24653139, Length=252, Percent_Identity=32.5396825396825, Blast_Score=125, Evalue=2e-29,
Organism=Drosophila melanogaster, GI24583165, Length=215, Percent_Identity=30.2325581395349, Blast_Score=96, Evalue=3e-20,
Organism=Drosophila melanogaster, GI19921018, Length=213, Percent_Identity=30.9859154929577, Blast_Score=93, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24650670, Length=242, Percent_Identity=30.1652892561983, Blast_Score=91, Evalue=6e-19,
Organism=Drosophila melanogaster, GI21357171, Length=203, Percent_Identity=31.5270935960591, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI45550169, Length=247, Percent_Identity=27.1255060728745, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI19920382, Length=206, Percent_Identity=27.6699029126214, Blast_Score=75, Evalue=4e-14,
Organism=Drosophila melanogaster, GI19922422, Length=261, Percent_Identity=24.5210727969349, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24583077, Length=193, Percent_Identity=33.160621761658, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24583079, Length=193, Percent_Identity=33.160621761658, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI19921000, Length=193, Percent_Identity=33.160621761658, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI28571729, Length=172, Percent_Identity=25.5813953488372, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI28571730, Length=172, Percent_Identity=25.5813953488372, Blast_Score=69, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 27664; Mature: 27533

Theoretical pI: Translated: 6.26; Mature: 6.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADRIEISIENHIAVMTIRRPEKLNALDIPLLQELSTACDQVEADSSVRVAILTGEGKAF
CCCEEEEEECCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCEE
SAGGDIKAWGGMEPQEFGHAWVRYGHRVFERLATLRVPLIAAMNGHALGGGLELAGTADI
ECCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCE
RIVERQAKIGLPETSLGMIPGWSGTQRLVRRFGAQIVRRMVLGGEMFTADEALSHGLVDH
EEEEHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
VVETGEALQAALAYAARVAKRGPAALEISKLMLSVANGEDNGTAVEALGSILAAKTGDLK
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCHH
EGVRSFTEKREANFEGKW
HHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
ADRIEISIENHIAVMTIRRPEKLNALDIPLLQELSTACDQVEADSSVRVAILTGEGKAF
CCEEEEEECCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCEE
SAGGDIKAWGGMEPQEFGHAWVRYGHRVFERLATLRVPLIAAMNGHALGGGLELAGTADI
ECCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCE
RIVERQAKIGLPETSLGMIPGWSGTQRLVRRFGAQIVRRMVLGGEMFTADEALSHGLVDH
EEEEHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
VVETGEALQAALAYAARVAKRGPAALEISKLMLSVANGEDNGTAVEALGSILAAKTGDLK
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCHH
EGVRSFTEKREANFEGKW
HHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA