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The map label for this gene is mdh [H]

Identifier: 23502777

GI number: 23502777

Start: 1860486

End: 1861448

Strand: Reverse

Name: mdh [H]

Synonym: BR1927

Alternate gene names: 23502777

Gene position: 1861448-1860486 (Counterclockwise)

Preceding gene: 23502778

Following gene: 23502776

Centisome position: 88.31

GC content: 61.68

Gene sequence:

>963_bases
ATGGCACGCAACAAGATTGCCCTCATCGGCTCCGGCATGATCGGCGGTACGCTCGCTCACCTGGCCGGTCTGAAGGAACT
CGGTGACGTCGTCCTTTTCGACATTGCGGAAGGCACCCCGCAGGGCAAGGGGCTGGATATCGCCGAATCTTCTCCGGTCG
ATGGTTTCGATGCGAAGTTCACTGGCGCGAACGATTACGCCGCCATTGAAGGCGCAGACGTCGTCATCGTCACCGCAGGC
GTGCCGCGCAAGCCGGGCATGAGCCGCGACGATCTCCTGGGCATCAACCTGAAGGTGATGGAACAGGTGGGCGCGGGCAT
CAAGAAATATGCGCCGGAAGCTTTCGTCATCTGCATCACCAACCCGCTCGACGCCATGGTCTGGGCGCTGCAGAAGTTCT
CCGGCCTTCCGGCCCACAAGGTTGTCGGCATGGCTGGCGTTCTCGACAGCGCCCGCTTCCGTTATTTCCTCTCGGAAGAA
TTCAACGTTTCGGTCGAGGACGTCACGGCATTCGTGCTGGGTGGCCACGGCGATTCGATGGTTCCGCTGGCGCGCTACTC
GACCGTTGCCGGCATTCCGCTGCCTGATCTCGTCAAGATGGGCTGGACCAGCCGGGACAAGCTCGACAAGATCATCCAGC
GCACCCGTGACGGCGGCGCGGAAATCGTGGGCCTTCTCAAGACCGGCTCGGCTTTCTACGCTCCGGCGGCATCGGCCATC
CAGGTGGCTGAATCCTACCTCAAGGACAAGAAGCGCGTCCTGCCGGTCGCAGCCCAGCTTTCGGGCCAGTATGGCGTGAA
GGACATGTATGTGGGCGTTCCGACCGTGATCGGCGCCAATGGCGTGGAGCGCATCATCGAGATCGATCTCGACAAGGACG
AGAAGGCGCAGTTCGACAAGTCGGTGGCCTCCGTCGCCGGTCTGTGCGAAGCCTGCATCGGTATTGCTCCGTCGCTGAAA
TAA

Upstream 100 bases:

>100_bases
AAGAGTCCTATCTCGCAGGTCCAACGCTTGAGTGTTTTCCGGGGAGGAAACCGGATGGGCTTGATGCGTCTCTGGATCAA
ATTTGAGGAAAGAAACAATC

Downstream 100 bases:

>100_bases
GTTCCAGACAAGGCCGGAATTCTGCTTGAGTGCGATTCCGGCCTTTTCCGGCTGTCAATCCGTACTGGCTCGTTGAACGG
GCCGGGAATTTGCAGAGGAC

Product: malate dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 320; Mature: 319

Protein sequence:

>320_residues
MARNKIALIGSGMIGGTLAHLAGLKELGDVVLFDIAEGTPQGKGLDIAESSPVDGFDAKFTGANDYAAIEGADVVIVTAG
VPRKPGMSRDDLLGINLKVMEQVGAGIKKYAPEAFVICITNPLDAMVWALQKFSGLPAHKVVGMAGVLDSARFRYFLSEE
FNVSVEDVTAFVLGGHGDSMVPLARYSTVAGIPLPDLVKMGWTSRDKLDKIIQRTRDGGAEIVGLLKTGSAFYAPAASAI
QVAESYLKDKKRVLPVAAQLSGQYGVKDMYVGVPTVIGANGVERIIEIDLDKDEKAQFDKSVASVAGLCEACIGIAPSLK

Sequences:

>Translated_320_residues
MARNKIALIGSGMIGGTLAHLAGLKELGDVVLFDIAEGTPQGKGLDIAESSPVDGFDAKFTGANDYAAIEGADVVIVTAG
VPRKPGMSRDDLLGINLKVMEQVGAGIKKYAPEAFVICITNPLDAMVWALQKFSGLPAHKVVGMAGVLDSARFRYFLSEE
FNVSVEDVTAFVLGGHGDSMVPLARYSTVAGIPLPDLVKMGWTSRDKLDKIIQRTRDGGAEIVGLLKTGSAFYAPAASAI
QVAESYLKDKKRVLPVAAQLSGQYGVKDMYVGVPTVIGANGVERIIEIDLDKDEKAQFDKSVASVAGLCEACIGIAPSLK
>Mature_319_residues
ARNKIALIGSGMIGGTLAHLAGLKELGDVVLFDIAEGTPQGKGLDIAESSPVDGFDAKFTGANDYAAIEGADVVIVTAGV
PRKPGMSRDDLLGINLKVMEQVGAGIKKYAPEAFVICITNPLDAMVWALQKFSGLPAHKVVGMAGVLDSARFRYFLSEEF
NVSVEDVTAFVLGGHGDSMVPLARYSTVAGIPLPDLVKMGWTSRDKLDKIIQRTRDGGAEIVGLLKTGSAFYAPAASAIQ
VAESYLKDKKRVLPVAAQLSGQYGVKDMYVGVPTVIGANGVERIIEIDLDKDEKAQFDKSVASVAGLCEACIGIAPSLK

Specific function: Catalyzes the reversible oxidation of malate to oxaloacetate [H]

COG id: COG0039

COG function: function code C; Malate/lactate dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LDH/MDH superfamily. MDH type 3 family [H]

Homologues:

Organism=Homo sapiens, GI47059044, Length=309, Percent_Identity=32.3624595469256, Blast_Score=176, Evalue=2e-44,
Organism=Homo sapiens, GI221136809, Length=309, Percent_Identity=32.3624595469256, Blast_Score=176, Evalue=2e-44,
Organism=Homo sapiens, GI9257228, Length=311, Percent_Identity=32.475884244373, Blast_Score=167, Evalue=1e-41,
Organism=Homo sapiens, GI4504973, Length=311, Percent_Identity=32.475884244373, Blast_Score=167, Evalue=1e-41,
Organism=Homo sapiens, GI5031857, Length=310, Percent_Identity=31.2903225806452, Blast_Score=162, Evalue=3e-40,
Organism=Homo sapiens, GI260099723, Length=310, Percent_Identity=31.2903225806452, Blast_Score=162, Evalue=4e-40,
Organism=Homo sapiens, GI15082234, Length=307, Percent_Identity=28.6644951140065, Blast_Score=158, Evalue=5e-39,
Organism=Homo sapiens, GI291575128, Length=303, Percent_Identity=31.6831683168317, Blast_Score=155, Evalue=5e-38,
Organism=Homo sapiens, GI4557032, Length=303, Percent_Identity=31.6831683168317, Blast_Score=155, Evalue=5e-38,
Organism=Homo sapiens, GI260099725, Length=206, Percent_Identity=33.0097087378641, Blast_Score=113, Evalue=2e-25,
Organism=Homo sapiens, GI260099727, Length=206, Percent_Identity=33.0097087378641, Blast_Score=113, Evalue=2e-25,
Organism=Homo sapiens, GI207028494, Length=193, Percent_Identity=32.6424870466321, Blast_Score=112, Evalue=6e-25,
Organism=Homo sapiens, GI21735621, Length=226, Percent_Identity=30.0884955752212, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI103472011, Length=304, Percent_Identity=22.0394736842105, Blast_Score=79, Evalue=7e-15,
Organism=Escherichia coli, GI1789632, Length=293, Percent_Identity=30.3754266211604, Blast_Score=86, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17535107, Length=243, Percent_Identity=28.3950617283951, Blast_Score=123, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI17554310, Length=314, Percent_Identity=31.5286624203822, Blast_Score=100, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6322765, Length=300, Percent_Identity=30, Blast_Score=92, Evalue=8e-20,
Organism=Saccharomyces cerevisiae, GI6320125, Length=253, Percent_Identity=27.2727272727273, Blast_Score=76, Evalue=8e-15,
Organism=Saccharomyces cerevisiae, GI6324446, Length=255, Percent_Identity=28.6274509803922, Blast_Score=75, Evalue=1e-14,
Organism=Drosophila melanogaster, GI17136226, Length=304, Percent_Identity=29.2763157894737, Blast_Score=146, Evalue=2e-35,
Organism=Drosophila melanogaster, GI45550422, Length=315, Percent_Identity=27.6190476190476, Blast_Score=114, Evalue=6e-26,
Organism=Drosophila melanogaster, GI24647881, Length=291, Percent_Identity=30.2405498281787, Blast_Score=93, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24663599, Length=309, Percent_Identity=27.1844660194175, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24663595, Length=260, Percent_Identity=28.8461538461538, Blast_Score=85, Evalue=8e-17,

Paralogues:

None

Copy number: 2640 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2380 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001557
- InterPro:   IPR022383
- InterPro:   IPR001236
- InterPro:   IPR015955
- InterPro:   IPR011275
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02866 Ldh_1_C; PF00056 Ldh_1_N [H]

EC number: =1.1.1.37 [H]

Molecular weight: Translated: 33672; Mature: 33541

Theoretical pI: Translated: 5.22; Mature: 5.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARNKIALIGSGMIGGTLAHLAGLKELGDVVLFDIAEGTPQGKGLDIAESSPVDGFDAKF
CCCCEEEEEECCCHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCEE
TGANDYAAIEGADVVIVTAGVPRKPGMSRDDLLGINLKVMEQVGAGIKKYAPEAFVICIT
CCCCCCEEECCCCEEEEECCCCCCCCCCCCCEECCHHHHHHHHCCCHHHHCCCEEEEEEC
NPLDAMVWALQKFSGLPAHKVVGMAGVLDSARFRYFLSEEFNVSVEDVTAFVLGGHGDSM
CHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHEEEECCCCCCCHHHEEEEEEECCCCCC
VPLARYSTVAGIPLPDLVKMGWTSRDKLDKIIQRTRDGGAEIVGLLKTGSAFYAPAASAI
CCHHHHHEECCCCCHHHHHCCCCCHHHHHHHHHHHCCCCCCEEEEEECCCEEECCHHHHH
QVAESYLKDKKRVLPVAAQLSGQYGVKDMYVGVPTVIGANGVERIIEIDLDKDEKAQFDK
HHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCEECCCCCEEEEEECCCCCHHHHHHH
SVASVAGLCEACIGIAPSLK
HHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
ARNKIALIGSGMIGGTLAHLAGLKELGDVVLFDIAEGTPQGKGLDIAESSPVDGFDAKF
CCCEEEEEECCCHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCEE
TGANDYAAIEGADVVIVTAGVPRKPGMSRDDLLGINLKVMEQVGAGIKKYAPEAFVICIT
CCCCCCEEECCCCEEEEECCCCCCCCCCCCCEECCHHHHHHHHCCCHHHHCCCEEEEEEC
NPLDAMVWALQKFSGLPAHKVVGMAGVLDSARFRYFLSEEFNVSVEDVTAFVLGGHGDSM
CHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHEEEECCCCCCCHHHEEEEEEECCCCCC
VPLARYSTVAGIPLPDLVKMGWTSRDKLDKIIQRTRDGGAEIVGLLKTGSAFYAPAASAI
CCHHHHHEECCCCCHHHHHCCCCCHHHHHHHHHHHCCCCCCEEEEEECCCEEECCHHHHH
QVAESYLKDKKRVLPVAAQLSGQYGVKDMYVGVPTVIGANGVERIIEIDLDKDEKAQFDK
HHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCEECCCCCEEEEEECCCCCHHHHHHH
SVASVAGLCEACIGIAPSLK
HHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA