Click here to switch to the map view.

The map label for this gene is trmD

Identifier: 23502764

GI number: 23502764

Start: 1846681

End: 1847415

Strand: Reverse

Name: trmD

Synonym: BR1914

Alternate gene names: 23502764

Gene position: 1847415-1846681 (Counterclockwise)

Preceding gene: 23502765

Following gene: 23502762

Centisome position: 87.65

GC content: 61.5

Gene sequence:

>735_bases
ATGACCGATTTGCCGGAAAAGGAAGGCGGGCGCTTTCATGCCTCCGTGCTGACGCTCTATCCTGAAATGTTTCCGGGGCC
TCTTGGCATCTCTCTTGCCGGGAAGGCGCTGGCCGAGGGCAAATGGCAGCTCGATACAGTGCAGATTCGCGATTTTGCCG
AGGGCAGGCATCGCATGGTCGATGATACTCCGTCGGGCGGCGGGGCAGGCATGGTGATGAAGGCCGATGTGGTGGCGCGG
GCGCTGGATTCGGTCGATGATGGGCGTCCCATGCTTTTGATGACGCCGCGCGGCAAGCCGCTGACGCAGGAGCGCGTGCG
TGCGCTGGCGGATGGGGCAGGGGCGATCATCCTGTGCGGCCGTTTCGAGGGCGTGGACGAGCGTGTCATCGAAGGGCGCA
ACCTCGAAGAAATCTCGATCGGCGATTATATTCTTTCCGGCGGGGAAACGGCGGCCATCGTGCTGCTCGACGCTGTGGTG
CGGCTTTTGCCGGGTGTGATGGGCAATCGCGAATCGGGTGAGACGGAAAGTTTCGAGACCGGCCTTCTCGAACACCCTCA
TTATACCCGCCCGCAGGAGTGGGAGGGCCGCGCCATTCCCGATATTCTGACATCGGGCAACCATGGCGCCATCGATAAAT
GGCGGCTGGAGCAGGCGGAGCGGATCACCAGAGAGCGTCGTCCCGATCTATGGGAAGCCTATTGCAAGAACAGGCGCAAG
ATTGGCGGTCAATAA

Upstream 100 bases:

>100_bases
TCGACAAGGGCATCATCAAGGTCGAGCCCCATGCGGCGGGCCTGATCGCCGATGAGCACGATAATCCGCCTCATGAAAGC
GGCAAGAAGCCGAAGAAGCC

Downstream 100 bases:

>100_bases
AAATGCCCGGAAGAGCCGGGCTGTGACTTTGATCGCTATTTGGCACTCATTATCAGCATTATGCGGCACTATTCAAACGG
CATCATTGGAACAAGAAGCT

Product: tRNA (guanine-N(1)-)-methyltransferase

Products: NA

Alternate protein names: M1G-methyltransferase; tRNA [GM37] methyltransferase

Number of amino acids: Translated: 244; Mature: 243

Protein sequence:

>244_residues
MTDLPEKEGGRFHASVLTLYPEMFPGPLGISLAGKALAEGKWQLDTVQIRDFAEGRHRMVDDTPSGGGAGMVMKADVVAR
ALDSVDDGRPMLLMTPRGKPLTQERVRALADGAGAIILCGRFEGVDERVIEGRNLEEISIGDYILSGGETAAIVLLDAVV
RLLPGVMGNRESGETESFETGLLEHPHYTRPQEWEGRAIPDILTSGNHGAIDKWRLEQAERITRERRPDLWEAYCKNRRK
IGGQ

Sequences:

>Translated_244_residues
MTDLPEKEGGRFHASVLTLYPEMFPGPLGISLAGKALAEGKWQLDTVQIRDFAEGRHRMVDDTPSGGGAGMVMKADVVAR
ALDSVDDGRPMLLMTPRGKPLTQERVRALADGAGAIILCGRFEGVDERVIEGRNLEEISIGDYILSGGETAAIVLLDAVV
RLLPGVMGNRESGETESFETGLLEHPHYTRPQEWEGRAIPDILTSGNHGAIDKWRLEQAERITRERRPDLWEAYCKNRRK
IGGQ
>Mature_243_residues
TDLPEKEGGRFHASVLTLYPEMFPGPLGISLAGKALAEGKWQLDTVQIRDFAEGRHRMVDDTPSGGGAGMVMKADVVARA
LDSVDDGRPMLLMTPRGKPLTQERVRALADGAGAIILCGRFEGVDERVIEGRNLEEISIGDYILSGGETAAIVLLDAVVR
LLPGVMGNRESGETESFETGLLEHPHYTRPQEWEGRAIPDILTSGNHGAIDKWRLEQAERITRERRPDLWEAYCKNRRKI
GGQ

Specific function: Specifically methylates guanosine-37 in various tRNAs

COG id: COG0336

COG function: function code J; tRNA-(guanine-N1)-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase trmD family

Homologues:

Organism=Escherichia coli, GI1788959, Length=221, Percent_Identity=43.8914027149321, Blast_Score=182, Evalue=2e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMD_BRUA1 (B2S868)

Other databases:

- EMBL:   CP000887
- RefSeq:   YP_001935751.1
- ProteinModelPortal:   B2S868
- SMR:   B2S868
- GeneID:   6326895
- GenomeReviews:   CP000887_GR
- KEGG:   bmc:BAbS19_I17950
- HOGENOM:   HBG285805
- OMA:   VCGRFEG
- ProtClustDB:   PRK00026
- GO:   GO:0005737
- HAMAP:   MF_00605
- InterPro:   IPR016009
- InterPro:   IPR002649
- InterPro:   IPR023148
- Gene3D:   G3DSA:1.10.1270.20
- PIRSF:   PIRSF000386
- TIGRFAMs:   TIGR00088

Pfam domain/function: PF01746 tRNA_m1G_MT

EC number: =2.1.1.31

Molecular weight: Translated: 26828; Mature: 26697

Theoretical pI: Translated: 5.08; Mature: 5.08

Prosite motif: NA

Important sites: BINDING 120-120

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDLPEKEGGRFHASVLTLYPEMFPGPLGISLAGKALAEGKWQLDTVQIRDFAEGRHRMV
CCCCCCCCCCEEEHHHHHHHHHHCCCCCCHHHHHHHHHCCCEEEEEEEHHHHHHHHHHCC
DDTPSGGGAGMVMKADVVARALDSVDDGRPMLLMTPRGKPLTQERVRALADGAGAIILCG
CCCCCCCCCCEEEHHHHHHHHHHHCCCCCCEEEECCCCCCCHHHHHHHHHCCCCEEEEEE
RFEGVDERVIEGRNLEEISIGDYILSGGETAAIVLLDAVVRLLPGVMGNRESGETESFET
CCCCCHHHHHCCCCCCEEECCCEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHH
GLLEHPHYTRPQEWEGRAIPDILTSGNHGAIDKWRLEQAERITRERRPDLWEAYCKNRRK
CCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHC
IGGQ
CCCC
>Mature Secondary Structure 
TDLPEKEGGRFHASVLTLYPEMFPGPLGISLAGKALAEGKWQLDTVQIRDFAEGRHRMV
CCCCCCCCCEEEHHHHHHHHHHCCCCCCHHHHHHHHHCCCEEEEEEEHHHHHHHHHHCC
DDTPSGGGAGMVMKADVVARALDSVDDGRPMLLMTPRGKPLTQERVRALADGAGAIILCG
CCCCCCCCCCEEEHHHHHHHHHHHCCCCCCEEEECCCCCCCHHHHHHHHHCCCCEEEEEE
RFEGVDERVIEGRNLEEISIGDYILSGGETAAIVLLDAVVRLLPGVMGNRESGETESFET
CCCCCHHHHHCCCCCCEEECCCEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHH
GLLEHPHYTRPQEWEGRAIPDILTSGNHGAIDKWRLEQAERITRERRPDLWEAYCKNRRK
CCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHC
IGGQ
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA