Click here to switch to the map view.

The map label for this gene is amt [H]

Identifier: 23502747

GI number: 23502747

Start: 1831408

End: 1832709

Strand: Reverse

Name: amt [H]

Synonym: BR1896

Alternate gene names: 23502747

Gene position: 1832709-1831408 (Counterclockwise)

Preceding gene: 23502749

Following gene: 23502746

Centisome position: 86.95

GC content: 59.14

Gene sequence:

>1302_bases
ATGCTCATAACAGGAAGAAACAAGCAAACGGCCCTCGTGGCCAGCCTGCCCGCACTTTTCGCCCTGGCGGGGACGGCAAT
GGCGCAGGACACCGCACCGGCACCAACCCTCGATACGGGCGATACGGCCTGGATGCTGACTTCAACCGCGCTTGTGTTGA
TGATGACCATACCCGGTCTGGCGCTGTTTTATGGCGGCATGGTGCGCAAGAAGAACGTGCTGTCCACGGTGATGCAAAGC
TTTGCCATCACCTGCCTCATGTCCATCCTGTGGATGATCGCAGGCTATTCGCTGGCCTTTACCGATGGCGGCTCGCTCCA
TTCCTATATTGGCGGCTTCTCGAAGGTTTTCTTCTCCGGAGTGACGATGGAATCGCTCACCGGAACCATCCCTGAATATC
TCTTCATCACCTTCCAGATGACGTTTGCGATCATCACGCCCGCGCTGATTACCGGCTCGTTTGCAGAGCGCATGAAGTTT
TCCTCCATGCTGGTTTTCCTGACGCTGTGGCTGTTCATCGTTTATGTTCCGGTTGCCCATTGGGTCTGGGGCGGGGGCTT
CATGGCTTCCGATGGCGTGCTTGATTTTGCCGGTGGCACGGTCGTTCATATCAATGCCGGTGTTGCCGGTCTGGTGGCAG
CCCTCGTTATCGGCAAGCGCGATGGTTATGGCCACACCAACATGGCGCCGCACAATCTCGTGCTCTCGGTCATCGGTGCT
GCCCTTCTGTGGGTGGGCTGGTTCGGCTTCAATGCCGGTTCTGCCGCCGGTGCCAATGCGCTTGCCGGTGTCGCCATGCT
CAACACGCAGGTCGCAACCGCCGGTGCTGCTCTTGCGTGGATGTTTGTGGAATGGGCTATTTCCGGCAAGCCAAGTGTGC
TCGGCATTATTTCCGGCGCTGTTGCGGGCCTTGTCGCGGTCACGCCCGCTGCCGGTTTTGTCAATCCGACCGGCGCGCTT
ATCATCGGCATCGTTGCCGGTGCGGTCTGCTATGTGGCGGCTGTGAAGATCAAGCATGTGCTGGGCTATGACGATTCGCT
CGATGCGTTCGGCGTCCATGGCGTCGGTGGTTTTGTCGGCGCGGTGCTGACCGGCTTTTTTGCCGATGCTACGATCAATC
CGGCCAGCGAAGGGGCAACCATCGGCAAGCAGTTCTTCGGCGCCGCCATCACGATCATTTATACGGCGATTGCCACCGTG
ATCATCCTTTACGTGGTCAAGGCCATCATGGGCTTGCGGCCCGGCAAGCAGGCCGAGATCGAGGGGCTGGATATCGCCTT
GCATGGGGAGGCGGTGCAGTGA

Upstream 100 bases:

>100_bases
CAGCACCTTCTCGCAACTGGCATGATTCTTGTTTGTCTCTGTTTGAACCGGCCTCTTCAGCGCAACCGCGTTTGAAGCCG
CCCTTCGACGGAGATATTCG

Downstream 100 bases:

>100_bases
GAACGGGAGTAGGGGAGTAGGAAGGTGTTTCCCTGCTTCCCCGGCTTTCGCAAGGTGCTGGCGAACCTTGCGCCTTTTCT
TTATTTGCCGGGCCTTATGC

Product: ammonium transporter

Products: NH3 [Cytoplasm] [C]

Alternate protein names: Ammonia transporter [H]

Number of amino acids: Translated: 433; Mature: 433

Protein sequence:

>433_residues
MLITGRNKQTALVASLPALFALAGTAMAQDTAPAPTLDTGDTAWMLTSTALVLMMTIPGLALFYGGMVRKKNVLSTVMQS
FAITCLMSILWMIAGYSLAFTDGGSLHSYIGGFSKVFFSGVTMESLTGTIPEYLFITFQMTFAIITPALITGSFAERMKF
SSMLVFLTLWLFIVYVPVAHWVWGGGFMASDGVLDFAGGTVVHINAGVAGLVAALVIGKRDGYGHTNMAPHNLVLSVIGA
ALLWVGWFGFNAGSAAGANALAGVAMLNTQVATAGAALAWMFVEWAISGKPSVLGIISGAVAGLVAVTPAAGFVNPTGAL
IIGIVAGAVCYVAAVKIKHVLGYDDSLDAFGVHGVGGFVGAVLTGFFADATINPASEGATIGKQFFGAAITIIYTAIATV
IILYVVKAIMGLRPGKQAEIEGLDIALHGEAVQ

Sequences:

>Translated_433_residues
MLITGRNKQTALVASLPALFALAGTAMAQDTAPAPTLDTGDTAWMLTSTALVLMMTIPGLALFYGGMVRKKNVLSTVMQS
FAITCLMSILWMIAGYSLAFTDGGSLHSYIGGFSKVFFSGVTMESLTGTIPEYLFITFQMTFAIITPALITGSFAERMKF
SSMLVFLTLWLFIVYVPVAHWVWGGGFMASDGVLDFAGGTVVHINAGVAGLVAALVIGKRDGYGHTNMAPHNLVLSVIGA
ALLWVGWFGFNAGSAAGANALAGVAMLNTQVATAGAALAWMFVEWAISGKPSVLGIISGAVAGLVAVTPAAGFVNPTGAL
IIGIVAGAVCYVAAVKIKHVLGYDDSLDAFGVHGVGGFVGAVLTGFFADATINPASEGATIGKQFFGAAITIIYTAIATV
IILYVVKAIMGLRPGKQAEIEGLDIALHGEAVQ
>Mature_433_residues
MLITGRNKQTALVASLPALFALAGTAMAQDTAPAPTLDTGDTAWMLTSTALVLMMTIPGLALFYGGMVRKKNVLSTVMQS
FAITCLMSILWMIAGYSLAFTDGGSLHSYIGGFSKVFFSGVTMESLTGTIPEYLFITFQMTFAIITPALITGSFAERMKF
SSMLVFLTLWLFIVYVPVAHWVWGGGFMASDGVLDFAGGTVVHINAGVAGLVAALVIGKRDGYGHTNMAPHNLVLSVIGA
ALLWVGWFGFNAGSAAGANALAGVAMLNTQVATAGAALAWMFVEWAISGKPSVLGIISGAVAGLVAVTPAAGFVNPTGAL
IIGIVAGAVCYVAAVKIKHVLGYDDSLDAFGVHGVGGFVGAVLTGFFADATINPASEGATIGKQFFGAAITIIYTAIATV
IILYVVKAIMGLRPGKQAEIEGLDIALHGEAVQ

Specific function: Involved in the uptake of ammonia [H]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=429, Percent_Identity=48.018648018648, Blast_Score=374, Evalue=1e-105,
Organism=Caenorhabditis elegans, GI17550182, Length=433, Percent_Identity=28.6374133949192, Blast_Score=142, Evalue=3e-34,
Organism=Caenorhabditis elegans, GI17550180, Length=447, Percent_Identity=27.9642058165548, Blast_Score=139, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI133931100, Length=435, Percent_Identity=26.4367816091954, Blast_Score=117, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI32564042, Length=428, Percent_Identity=25.9345794392523, Blast_Score=115, Evalue=5e-26,
Organism=Caenorhabditis elegans, GI17563228, Length=194, Percent_Identity=30.4123711340206, Blast_Score=69, Evalue=6e-12,
Organism=Saccharomyces cerevisiae, GI6324187, Length=417, Percent_Identity=37.410071942446, Blast_Score=239, Evalue=4e-64,
Organism=Saccharomyces cerevisiae, GI6325396, Length=412, Percent_Identity=34.9514563106796, Blast_Score=220, Evalue=3e-58,
Organism=Saccharomyces cerevisiae, GI6321559, Length=421, Percent_Identity=34.4418052256532, Blast_Score=214, Evalue=2e-56,
Organism=Drosophila melanogaster, GI161078314, Length=416, Percent_Identity=27.1634615384615, Blast_Score=126, Evalue=4e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 44696; Mature: 44696

Theoretical pI: Translated: 6.72; Mature: 6.72

Prosite motif: PS01219 AMMONIUM_TRANSP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLITGRNKQTALVASLPALFALAGTAMAQDTAPAPTLDTGDTAWMLTSTALVLMMTIPGL
CEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
ALFYGGMVRKKNVLSTVMQSFAITCLMSILWMIAGYSLAFTDGGSLHSYIGGFSKVFFSG
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHCC
VTMESLTGTIPEYLFITFQMTFAIITPALITGSFAERMKFSSMLVFLTLWLFIVYVPVAH
CCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WVWGGGFMASDGVLDFAGGTVVHINAGVAGLVAALVIGKRDGYGHTNMAPHNLVLSVIGA
HHHCCCEEECCCCEEECCCEEEEECCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHH
ALLWVGWFGFNAGSAAGANALAGVAMLNTQVATAGAALAWMFVEWAISGKPSVLGIISGA
HHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
VAGLVAVTPAAGFVNPTGALIIGIVAGAVCYVAAVKIKHVLGYDDSLDAFGVHGVGGFVG
HHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHH
AVLTGFFADATINPASEGATIGKQFFGAAITIIYTAIATVIILYVVKAIMGLRPGKQAEI
HHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
EGLDIALHGEAVQ
CCEEEEECCCCCC
>Mature Secondary Structure
MLITGRNKQTALVASLPALFALAGTAMAQDTAPAPTLDTGDTAWMLTSTALVLMMTIPGL
CEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
ALFYGGMVRKKNVLSTVMQSFAITCLMSILWMIAGYSLAFTDGGSLHSYIGGFSKVFFSG
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHCC
VTMESLTGTIPEYLFITFQMTFAIITPALITGSFAERMKFSSMLVFLTLWLFIVYVPVAH
CCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WVWGGGFMASDGVLDFAGGTVVHINAGVAGLVAALVIGKRDGYGHTNMAPHNLVLSVIGA
HHHCCCEEECCCCEEECCCEEEEECCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHH
ALLWVGWFGFNAGSAAGANALAGVAMLNTQVATAGAALAWMFVEWAISGKPSVLGIISGA
HHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
VAGLVAVTPAAGFVNPTGALIIGIVAGAVCYVAAVKIKHVLGYDDSLDAFGVHGVGGFVG
HHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHH
AVLTGFFADATINPASEGATIGKQFFGAAITIIYTAIATVIILYVVKAIMGLRPGKQAEI
HHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
EGLDIALHGEAVQ
CCEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]