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The map label for this gene is prfA [H]

Identifier: 23502722

GI number: 23502722

Start: 1804934

End: 1806013

Strand: Reverse

Name: prfA [H]

Synonym: BR1869

Alternate gene names: 23502722

Gene position: 1806013-1804934 (Counterclockwise)

Preceding gene: 23502723

Following gene: 23502721

Centisome position: 85.68

GC content: 60.19

Gene sequence:

>1080_bases
ATGATCGCATTGCCCCAGGACCGAATGGATCAGCTTTTGAAGCGGTTCTCGATGATTGAAAGCCAGATGGCCAACAATCC
CGATTCGGATACCTATGTGAAGCTTGCCTCCGAATATTCCGAACTCCAGGACGTGGTGGGCAAAATCCGCGAACTGAGCG
ATGCGCGCATGGAAGCAAGCGACCTTGCCGCGATGCGCGACGATGCTTCGACGGATGCGGAGATGCGCGCTCTGGCGGTG
GAGGAACTGCCGGAGGTGGAAAAGCGCATCGCGGTGCTGGAACAGGATGTGCAAATCCTGCTTCTGCCCAAGGATGCCGC
CGACGACAAGAATGCGATCCTTGAAATTCGCGCCGGTACGGGCGGTCTGGAAGCTGCCCTTTTCGCGGGCGATCTGTTCC
GCATGTATGAACGCTATGCGGCTGAAAAAGGCTGGCGGGTGGAACTTGTTTCGGCCAGCGAGGGCGATGCGGGCGGCTAT
AAGGAAATCATCGCCACGGTTTCGGGCAAGGGGGTCTTCTCAAAGCTGAAGTTTGAATCCGGCGTGCATCGCGTGCAGCG
CGTGCCGGAAACGGAAGCGGGCGGGCGCATCCACACATCGGCAGCCACGGTTGCCGTTCTGCCGGAAGCGGAAGATATCG
ATATTGAAATTCGCAACGAGGATATCCGGATCGATACGATGCGCGCCTCGGGCGCGGGCGGGCAGCACGTCAATACGACC
GATTCGGCTGTCCGAATCACGCATATCCCGACCGGCATCATGGTGGTGCAGGCGGAAAAATCGCAGCACCAGAACCGTGC
CCGCGCCATGCAGATCCTGCGCGCCCGCCTTTATGACATGGAGCGCCAGAAGGCTGAAAGCGAACGCTCGCAGGCGCGCC
GCAGCCAGGTTGGTTCGGGCGACCGGTCGGAGCGCATCCGTACCTATAATTTCCCGCAGGGGCGCGTCACCGACCATCGG
ATCAATCTCACGCTCTACAAGCTCGACCGCGTGATGGAAGGCGATCTGGACGAACTGGTCGATGCGCTGATTTCCGATCA
CCAGACCGCCCTTCTGGCCGAACTGGGCGAGCAGCCGTGA

Upstream 100 bases:

>100_bases
GAATCGTCGAACCGCTCTCATTATTCGTTATTGTCGCATTATCCGTATGCAAAACCGCTTCATGCTTTTGCCGGAAATGC
TCCAGTTTCTAGGACACGAC

Downstream 100 bases:

>100_bases
GCAGCGAAGAGGTGGAGCGCCTTGACCGGCTGATGGCAGAGGCCCGCGCGAAATTGCGTGCGGCGGGGGGCGAGACGCCC
GATCTCGATGCGCGTCTGCT

Product: peptide chain release factor 1

Products: NA

Alternate protein names: RF-1 [H]

Number of amino acids: Translated: 359; Mature: 359

Protein sequence:

>359_residues
MIALPQDRMDQLLKRFSMIESQMANNPDSDTYVKLASEYSELQDVVGKIRELSDARMEASDLAAMRDDASTDAEMRALAV
EELPEVEKRIAVLEQDVQILLLPKDAADDKNAILEIRAGTGGLEAALFAGDLFRMYERYAAEKGWRVELVSASEGDAGGY
KEIIATVSGKGVFSKLKFESGVHRVQRVPETEAGGRIHTSAATVAVLPEAEDIDIEIRNEDIRIDTMRASGAGGQHVNTT
DSAVRITHIPTGIMVVQAEKSQHQNRARAMQILRARLYDMERQKAESERSQARRSQVGSGDRSERIRTYNFPQGRVTDHR
INLTLYKLDRVMEGDLDELVDALISDHQTALLAELGEQP

Sequences:

>Translated_359_residues
MIALPQDRMDQLLKRFSMIESQMANNPDSDTYVKLASEYSELQDVVGKIRELSDARMEASDLAAMRDDASTDAEMRALAV
EELPEVEKRIAVLEQDVQILLLPKDAADDKNAILEIRAGTGGLEAALFAGDLFRMYERYAAEKGWRVELVSASEGDAGGY
KEIIATVSGKGVFSKLKFESGVHRVQRVPETEAGGRIHTSAATVAVLPEAEDIDIEIRNEDIRIDTMRASGAGGQHVNTT
DSAVRITHIPTGIMVVQAEKSQHQNRARAMQILRARLYDMERQKAESERSQARRSQVGSGDRSERIRTYNFPQGRVTDHR
INLTLYKLDRVMEGDLDELVDALISDHQTALLAELGEQP
>Mature_359_residues
MIALPQDRMDQLLKRFSMIESQMANNPDSDTYVKLASEYSELQDVVGKIRELSDARMEASDLAAMRDDASTDAEMRALAV
EELPEVEKRIAVLEQDVQILLLPKDAADDKNAILEIRAGTGGLEAALFAGDLFRMYERYAAEKGWRVELVSASEGDAGGY
KEIIATVSGKGVFSKLKFESGVHRVQRVPETEAGGRIHTSAATVAVLPEAEDIDIEIRNEDIRIDTMRASGAGGQHVNTT
DSAVRITHIPTGIMVVQAEKSQHQNRARAMQILRARLYDMERQKAESERSQARRSQVGSGDRSERIRTYNFPQGRVTDHR
INLTLYKLDRVMEGDLDELVDALISDHQTALLAELGEQP

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA [H]

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=303, Percent_Identity=47.5247524752475, Blast_Score=284, Evalue=1e-76,
Organism=Homo sapiens, GI34577120, Length=361, Percent_Identity=37.6731301939058, Blast_Score=245, Evalue=6e-65,
Organism=Homo sapiens, GI166795305, Length=243, Percent_Identity=39.917695473251, Blast_Score=189, Evalue=3e-48,
Organism=Escherichia coli, GI1787462, Length=335, Percent_Identity=58.8059701492537, Blast_Score=364, Evalue=1e-102,
Organism=Escherichia coli, GI2367172, Length=315, Percent_Identity=39.0476190476191, Blast_Score=202, Evalue=4e-53,
Organism=Caenorhabditis elegans, GI17542784, Length=281, Percent_Identity=39.5017793594306, Blast_Score=187, Evalue=7e-48,
Organism=Saccharomyces cerevisiae, GI6321295, Length=316, Percent_Identity=43.9873417721519, Blast_Score=246, Evalue=5e-66,
Organism=Drosophila melanogaster, GI19921226, Length=326, Percent_Identity=42.3312883435583, Blast_Score=230, Evalue=1e-60,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 39895; Mature: 39895

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIALPQDRMDQLLKRFSMIESQMANNPDSDTYVKLASEYSELQDVVGKIRELSDARMEAS
CCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DLAAMRDDASTDAEMRALAVEELPEVEKRIAVLEQDVQILLLPKDAADDKNAILEIRAGT
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCEEEEECCC
GGLEAALFAGDLFRMYERYAAEKGWRVELVSASEGDAGGYKEIIATVSGKGVFSKLKFES
CCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHCCCCHHHHHHHHH
GVHRVQRVPETEAGGRIHTSAATVAVLPEAEDIDIEIRNEDIRIDTMRASGAGGQHVNTT
HHHHHHHCCCCCCCCEEEECCEEEEEECCCCCCEEEEECCCEEEEEEEECCCCCCCCCCC
DSAVRITHIPTGIMVVQAEKSQHQNRARAMQILRARLYDMERQKAESERSQARRSQVGSG
CCEEEEEECCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
DRSERIRTYNFPQGRVTDHRINLTLYKLDRVMEGDLDELVDALISDHQTALLAELGEQP
CCCCCCEEECCCCCCCCCEEEEEEEEEHHHHHCCCHHHHHHHHHCCCHHHHHHHHCCCC
>Mature Secondary Structure
MIALPQDRMDQLLKRFSMIESQMANNPDSDTYVKLASEYSELQDVVGKIRELSDARMEAS
CCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DLAAMRDDASTDAEMRALAVEELPEVEKRIAVLEQDVQILLLPKDAADDKNAILEIRAGT
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCEEEEECCC
GGLEAALFAGDLFRMYERYAAEKGWRVELVSASEGDAGGYKEIIATVSGKGVFSKLKFES
CCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHCCCCHHHHHHHHH
GVHRVQRVPETEAGGRIHTSAATVAVLPEAEDIDIEIRNEDIRIDTMRASGAGGQHVNTT
HHHHHHHCCCCCCCCEEEECCEEEEEECCCCCCEEEEECCCEEEEEEEECCCCCCCCCCC
DSAVRITHIPTGIMVVQAEKSQHQNRARAMQILRARLYDMERQKAESERSQARRSQVGSG
CCEEEEEECCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
DRSERIRTYNFPQGRVTDHRINLTLYKLDRVMEGDLDELVDALISDHQTALLAELGEQP
CCCCCCEEECCCCCCCCCEEEEEEEEEHHHHHCCCHHHHHHHHHCCCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA