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The map label for this gene is potF [H]

Identifier: 23502475

GI number: 23502475

Start: 1558863

End: 1559966

Strand: Reverse

Name: potF [H]

Synonym: BR1612

Alternate gene names: 23502475

Gene position: 1559966-1558863 (Counterclockwise)

Preceding gene: 23502476

Following gene: 23502474

Centisome position: 74.01

GC content: 55.53

Gene sequence:

>1104_bases
ATGGGGATCAAATCCTTCCTTCTGGCAACGACCGTTGCGACGGGTTTTGTCGCGGCTGCCACATTTTCCGCAGGCGCGCA
GGAGCGGGTGGTCAATATCTATAACTGGTCGGATTATATCGACGATTCCATCCTCAAGGACTTCACCAAGGAGACCGGGA
TCAAGGTCGTCTACGACGTCTATGACTCCAACGAAATTCTGGAAACCAAGCTTCTGGCGGGCGGCAGCGGCTACGACCTC
GTGGTGCCATCGGGCGAATTTCTTGGGCGCCAGATTCCCGCAGGCGTGTTCCTGAAACTCGACAAGGACAAGCTGCCGAA
CCTCAAGAATATGTGGGATGAGATTTCGACCCGTGCGGCAACCTATGATCCGGGCAACGAATATTCCGCCAATTACATGT
GGGGCACGACCGGCATCGGCTACAATAAGGCCAAGATCAAGGAAGCGCTCGGCACCGACACGATCGACTCCTGGGACGTG
CTTTTCGATCCGGAAAAAACGGCAAAGCTGAAAGATTGCGGCATTTACCTGCTTGATTCCGCCAGCGAAATGCTGCGTCC
GGCGCTGAACTATCTGGGTCTCGACCCGAACTCTCCGTCGCCGGACGATTTGCAGAAGGCACAGGATCTCTATCTCAAGA
TTCGTCCGAATATCCGCAAATTCCACTCGTCGGAATATATCAATGCGCTCGCCAATGGCGATATCTGCATGGCTGTCGGC
TATTCCGGCGATATTTTCCAGGCCCGCGACCGCGCCGAAAAGGCGAAGCAGGGGGTGGAGATCGGCTATTCGATCCCGAA
GGAAGGCGCGCTGATCTGGTTTGACCAGATGGCGATCCCGGCCGATGCCAAGCATGTGCCGGAGGCGTTGGAATTCATGA
ATTATATGATGCGCCCGGAGGTGGCGGCAAAGGCGTCGAACTATGTGTTCTATGCCAATGGCAACAAGGCGTCGCAGAAA
TTCATCGACAAGGAAATCCTCGACGACCCGGAAATCTATCCGTCCGACGAGGTGATGAAGAAGCTGTTCGTGCCGACGCC
ATATGACACGAAGACCCAGCGTGTGGTCACACGCGCCTGGACCAAGATCGTCACCGGCCAGTAA

Upstream 100 bases:

>100_bases
CCTGTTGACGAATGCCGTGCAATCTAAAAAAGTGCCATAAAGAAAGAATATATCCACCTTGAACCTGCACCGAATTTTTA
TGTGCAGATTGGAGCAAATG

Downstream 100 bases:

>100_bases
AGCCAAATGACCATGGGACCGATATGTCCCGCCAATATCGTGAAGAGCGGCCCACTGTGTAGACGGAGGGCCGCTCTGCG
TCTTGTAACGAGTGGGTTTC

Product: spermidine/putrescine ABC transporter periplasmic spermidine/putrescine-binding protein

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 367; Mature: 366

Protein sequence:

>367_residues
MGIKSFLLATTVATGFVAAATFSAGAQERVVNIYNWSDYIDDSILKDFTKETGIKVVYDVYDSNEILETKLLAGGSGYDL
VVPSGEFLGRQIPAGVFLKLDKDKLPNLKNMWDEISTRAATYDPGNEYSANYMWGTTGIGYNKAKIKEALGTDTIDSWDV
LFDPEKTAKLKDCGIYLLDSASEMLRPALNYLGLDPNSPSPDDLQKAQDLYLKIRPNIRKFHSSEYINALANGDICMAVG
YSGDIFQARDRAEKAKQGVEIGYSIPKEGALIWFDQMAIPADAKHVPEALEFMNYMMRPEVAAKASNYVFYANGNKASQK
FIDKEILDDPEIYPSDEVMKKLFVPTPYDTKTQRVVTRAWTKIVTGQ

Sequences:

>Translated_367_residues
MGIKSFLLATTVATGFVAAATFSAGAQERVVNIYNWSDYIDDSILKDFTKETGIKVVYDVYDSNEILETKLLAGGSGYDL
VVPSGEFLGRQIPAGVFLKLDKDKLPNLKNMWDEISTRAATYDPGNEYSANYMWGTTGIGYNKAKIKEALGTDTIDSWDV
LFDPEKTAKLKDCGIYLLDSASEMLRPALNYLGLDPNSPSPDDLQKAQDLYLKIRPNIRKFHSSEYINALANGDICMAVG
YSGDIFQARDRAEKAKQGVEIGYSIPKEGALIWFDQMAIPADAKHVPEALEFMNYMMRPEVAAKASNYVFYANGNKASQK
FIDKEILDDPEIYPSDEVMKKLFVPTPYDTKTQRVVTRAWTKIVTGQ
>Mature_366_residues
GIKSFLLATTVATGFVAAATFSAGAQERVVNIYNWSDYIDDSILKDFTKETGIKVVYDVYDSNEILETKLLAGGSGYDLV
VPSGEFLGRQIPAGVFLKLDKDKLPNLKNMWDEISTRAATYDPGNEYSANYMWGTTGIGYNKAKIKEALGTDTIDSWDVL
FDPEKTAKLKDCGIYLLDSASEMLRPALNYLGLDPNSPSPDDLQKAQDLYLKIRPNIRKFHSSEYINALANGDICMAVGY
SGDIFQARDRAEKAKQGVEIGYSIPKEGALIWFDQMAIPADAKHVPEALEFMNYMMRPEVAAKASNYVFYANGNKASQKF
IDKEILDDPEIYPSDEVMKKLFVPTPYDTKTQRVVTRAWTKIVTGQ

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine. Polyamine binding protein [H]

COG id: COG0687

COG function: function code E; Spermidine/putrescine-binding periplasmic protein

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787078, Length=362, Percent_Identity=52.7624309392265, Blast_Score=394, Evalue=1e-111,
Organism=Escherichia coli, GI1787367, Length=309, Percent_Identity=35.2750809061489, Blast_Score=191, Evalue=7e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 40892; Mature: 40761

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGIKSFLLATTVATGFVAAATFSAGAQERVVNIYNWSDYIDDSILKDFTKETGIKVVYDV
CCHHHHHHHHHHHHHHHHHHHHCCCCHHHEEEEECCHHHHHHHHHHHHHHHCCCEEEEEE
YDSNEILETKLLAGGSGYDLVVPSGEFLGRQIPAGVFLKLDKDKLPNLKNMWDEISTRAA
CCCCHHHHHHEECCCCCCEEEECCHHHHHCCCCCCEEEEECHHHCCCHHHHHHHHHHCCC
TYDPGNEYSANYMWGTTGIGYNKAKIKEALGTDTIDSWDVLFDPEKTAKLKDCGIYLLDS
CCCCCCCCCCCEEEECCCCCCCHHHHHHHHCCCCCCCCCEEECCCHHCCHHHCCEEEECC
ASEMLRPALNYLGLDPNSPSPDDLQKAQDLYLKIRPNIRKFHSSEYINALANGDICMAVG
HHHHHHHHHHHCCCCCCCCCHHHHHHHHHEEEEECCCHHHHHHHHHHHHHCCCCEEEEEC
YSGDIFQARDRAEKAKQGVEIGYSIPKEGALIWFDQMAIPADAKHVPEALEFMNYMMRPE
CCCCHHHHHHHHHHHHCCCEECCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHCCH
VAAKASNYVFYANGNKASQKFIDKEILDDPEIYPSDEVMKKLFVPTPYDTKTQRVVTRAW
HHHCCCCEEEEECCCHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHH
TKIVTGQ
HHHHCCC
>Mature Secondary Structure 
GIKSFLLATTVATGFVAAATFSAGAQERVVNIYNWSDYIDDSILKDFTKETGIKVVYDV
CHHHHHHHHHHHHHHHHHHHHCCCCHHHEEEEECCHHHHHHHHHHHHHHHCCCEEEEEE
YDSNEILETKLLAGGSGYDLVVPSGEFLGRQIPAGVFLKLDKDKLPNLKNMWDEISTRAA
CCCCHHHHHHEECCCCCCEEEECCHHHHHCCCCCCEEEEECHHHCCCHHHHHHHHHHCCC
TYDPGNEYSANYMWGTTGIGYNKAKIKEALGTDTIDSWDVLFDPEKTAKLKDCGIYLLDS
CCCCCCCCCCCEEEECCCCCCCHHHHHHHHCCCCCCCCCEEECCCHHCCHHHCCEEEECC
ASEMLRPALNYLGLDPNSPSPDDLQKAQDLYLKIRPNIRKFHSSEYINALANGDICMAVG
HHHHHHHHHHHCCCCCCCCCHHHHHHHHHEEEEECCCHHHHHHHHHHHHHCCCCEEEEEC
YSGDIFQARDRAEKAKQGVEIGYSIPKEGALIWFDQMAIPADAKHVPEALEFMNYMMRPE
CCCCHHHHHHHHHHHHCCCEECCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHCCH
VAAKASNYVFYANGNKASQKFIDKEILDDPEIYPSDEVMKKLFVPTPYDTKTQRVVTRAW
HHHCCCCEEEEECCCHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHH
TKIVTGQ
HHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: Polyamine Binding Protein [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8416922; 8905232; 9278503; 9298646; 9651355 [H]