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The map label for this gene is yafC [H]

Identifier: 23502367

GI number: 23502367

Start: 1453395

End: 1454300

Strand: Reverse

Name: yafC [H]

Synonym: BR1498

Alternate gene names: 23502367

Gene position: 1454300-1453395 (Counterclockwise)

Preceding gene: 23502368

Following gene: 23502363

Centisome position: 69.0

GC content: 57.17

Gene sequence:

>906_bases
GTGGTCGCACCGCTTGACTGGGATAAACTGCGCATATTCCACGCGGCGGCAGAGGCTGGTTCGTTCACCCATGCCGCCCA
GACACTGCACCTGTCGCAATCAGCTATTTCGCGCCAGGTCAGTGCGCTGGAACAGGATGTGGGCGTTCCGCTTTTTCATC
GCCATGCGCGCGGGCTTATTCTGACGGAGCAGGGCGAAACGCTTTATCGTACGGCGCATGATGTGCTGATGAAGCTGGAA
AATGTCCGCTCCAAACTGGCGGAAAGCCGTGAAAAACCCTCTGGCCGTCTGCGGGTCACGACAACTGTTGGCCTCGGTTC
GGGCTGGCTGATCGAGCGTATCCAGGAATTTGTCGAGCTTTATCCCGATGTGCAGCTTCAGCTTATTCTCGATAATGAGG
AGCTGGACCTGACCATGCGGCATGCCGACTGTGCGGTGCGCCTGCGCCAGCCACAGCAGCCGGATCTCATTCAGCGCCGC
CTGTTCATTGTCCATATGCATGTTTATGCTTCGGCAGGCTATGTCTCCAAATATGGTAAGTTGAACTCTATCGATGAGAT
CGACCAGCACCGGATCGTGACTTTCGGTGAGCCTGCGCCGAGCTATCTGACGGGGCTTAACTGGCTGGAGACCGCCGGGC
GACCCGATGGTAGCGCACGCATTCCGGCCTTGCAGGTGAACAATCTCCTGTCTGTGCGCCGTGCGGTGCAGCGCGGGGTC
GGTATTGCGGTCCTGCCCGACTATATGGCCGATAAGGAATCCGGTCTGGTGCAGCTTCTGCCCGAGCTTGAGGAAATACC
GTCTTTCGATACGTTTTTCTGCTATCCAGAGGCTTTGAAGAATTCGGCCAAGCTCCATGCCTTCCGCGATTTCCTGTTCT
CGAAGGCCCGCAATTGGACCTATTGA

Upstream 100 bases:

>100_bases
CAGGAGCCGCTGCACGAAGCGGCGGAGTAGAGCCTTCCGGCAAAAGTGCGAAGCGTTTTACGGAGGGTAAATGCGGTGAA
AACAATGAGAGGGGAAAAAC

Downstream 100 bases:

>100_bases
CGGCCGCAGACGCCGGAATTTTTCCGCGCTGTCATATGAGCCGTCTATATTTCAATTGCCGGTTTTGAAGGCAGCCTTTT
GGGGGTGTGTGCCCGGTATT

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 301; Mature: 301

Protein sequence:

>301_residues
MVAPLDWDKLRIFHAAAEAGSFTHAAQTLHLSQSAISRQVSALEQDVGVPLFHRHARGLILTEQGETLYRTAHDVLMKLE
NVRSKLAESREKPSGRLRVTTTVGLGSGWLIERIQEFVELYPDVQLQLILDNEELDLTMRHADCAVRLRQPQQPDLIQRR
LFIVHMHVYASAGYVSKYGKLNSIDEIDQHRIVTFGEPAPSYLTGLNWLETAGRPDGSARIPALQVNNLLSVRRAVQRGV
GIAVLPDYMADKESGLVQLLPELEEIPSFDTFFCYPEALKNSAKLHAFRDFLFSKARNWTY

Sequences:

>Translated_301_residues
MVAPLDWDKLRIFHAAAEAGSFTHAAQTLHLSQSAISRQVSALEQDVGVPLFHRHARGLILTEQGETLYRTAHDVLMKLE
NVRSKLAESREKPSGRLRVTTTVGLGSGWLIERIQEFVELYPDVQLQLILDNEELDLTMRHADCAVRLRQPQQPDLIQRR
LFIVHMHVYASAGYVSKYGKLNSIDEIDQHRIVTFGEPAPSYLTGLNWLETAGRPDGSARIPALQVNNLLSVRRAVQRGV
GIAVLPDYMADKESGLVQLLPELEEIPSFDTFFCYPEALKNSAKLHAFRDFLFSKARNWTY
>Mature_301_residues
MVAPLDWDKLRIFHAAAEAGSFTHAAQTLHLSQSAISRQVSALEQDVGVPLFHRHARGLILTEQGETLYRTAHDVLMKLE
NVRSKLAESREKPSGRLRVTTTVGLGSGWLIERIQEFVELYPDVQLQLILDNEELDLTMRHADCAVRLRQPQQPDLIQRR
LFIVHMHVYASAGYVSKYGKLNSIDEIDQHRIVTFGEPAPSYLTGLNWLETAGRPDGSARIPALQVNNLLSVRRAVQRGV
GIAVLPDYMADKESGLVQLLPELEEIPSFDTFFCYPEALKNSAKLHAFRDFLFSKARNWTY

Specific function: Unknown

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786401, Length=293, Percent_Identity=34.8122866894198, Blast_Score=143, Evalue=1e-35,
Organism=Escherichia coli, GI145693193, Length=264, Percent_Identity=29.1666666666667, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1789440, Length=294, Percent_Identity=27.2108843537415, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1789639, Length=251, Percent_Identity=27.8884462151394, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI87081978, Length=288, Percent_Identity=26.0416666666667, Blast_Score=95, Evalue=6e-21,
Organism=Escherichia coli, GI1787589, Length=293, Percent_Identity=26.962457337884, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1787128, Length=296, Percent_Identity=27.027027027027, Blast_Score=88, Evalue=7e-19,
Organism=Escherichia coli, GI1789173, Length=296, Percent_Identity=25, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI157672245, Length=116, Percent_Identity=37.9310344827586, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI1788887, Length=295, Percent_Identity=26.7796610169492, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1788706, Length=176, Percent_Identity=26.1363636363636, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1790262, Length=140, Percent_Identity=34.2857142857143, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1789204, Length=128, Percent_Identity=32.03125, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1788626, Length=287, Percent_Identity=26.4808362369338, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1786448, Length=131, Percent_Identity=33.587786259542, Blast_Score=64, Evalue=9e-12,
Organism=Escherichia coli, GI1788296, Length=127, Percent_Identity=33.8582677165354, Blast_Score=62, Evalue=4e-11,
Organism=Escherichia coli, GI1790208, Length=124, Percent_Identity=32.258064516129, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34027; Mature: 34027

Theoretical pI: Translated: 6.92; Mature: 6.92

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVAPLDWDKLRIFHAAAEAGSFTHAAQTLHLSQSAISRQVSALEQDVGVPLFHRHARGLI
CCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCEE
LTEQGETLYRTAHDVLMKLENVRSKLAESREKPSGRLRVTTTVGLGSGWLIERIQEFVEL
EECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCHHHHHHHHHHHH
YPDVQLQLILDNEELDLTMRHADCAVRLRQPQQPDLIQRRLFIVHMHVYASAGYVSKYGK
CCCCEEEEEECCCCCEEEEECHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHCCC
LNSIDEIDQHRIVTFGEPAPSYLTGLNWLETAGRPDGSARIPALQVNNLLSVRRAVQRGV
CCCHHHHCCCEEEEECCCCCHHHHCCHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCC
GIAVLPDYMADKESGLVQLLPELEEIPSFDTFFCYPEALKNSAKLHAFRDFLFSKARNWT
CEEECCHHHCCCCCCHHHHHHHHHHCCCCCCEEECHHHHCCCHHHHHHHHHHHHHHCCCC
Y
C
>Mature Secondary Structure
MVAPLDWDKLRIFHAAAEAGSFTHAAQTLHLSQSAISRQVSALEQDVGVPLFHRHARGLI
CCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCEE
LTEQGETLYRTAHDVLMKLENVRSKLAESREKPSGRLRVTTTVGLGSGWLIERIQEFVEL
EECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCHHHHHHHHHHHH
YPDVQLQLILDNEELDLTMRHADCAVRLRQPQQPDLIQRRLFIVHMHVYASAGYVSKYGK
CCCCEEEEEECCCCCEEEEECHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHCCC
LNSIDEIDQHRIVTFGEPAPSYLTGLNWLETAGRPDGSARIPALQVNNLLSVRRAVQRGV
CCCHHHHCCCEEEEECCCCCHHHHCCHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCC
GIAVLPDYMADKESGLVQLLPELEEIPSFDTFFCYPEALKNSAKLHAFRDFLFSKARNWT
CEEECCHHHCCCCCCHHHHHHHHHHCCCCCCEEECHHHHCCCHHHHHHHHHHHHHHCCCC
Y
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]