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The map label for this gene is dnaG [H]

Identifier: 23502349

GI number: 23502349

Start: 1434620

End: 1436587

Strand: Reverse

Name: dnaG [H]

Synonym: BR1480

Alternate gene names: 23502349

Gene position: 1436587-1434620 (Counterclockwise)

Preceding gene: 23502351

Following gene: 23502348

Centisome position: 68.16

GC content: 61.59

Gene sequence:

>1968_bases
ATGCGTTTTCCGCCCTCCTTTCTCGATGAGATCAGAGACCGCGTCCCGATCTCGACGCTGATCGGAACGCGTGTTTCATT
TGACCGCAAGAAAAGCAATCCGCCCAAGGGCGATTTCTGGGCCTGCTGTCCGTTTCATGGCGAGAAGACGCCGAGCTTTC
ATTGCGAGGACCGCAAGGGCCGTTACCACTGTTTCGGTTGCGGTGTGACTGGCGATCACTTCAAGTTCCTGACAGACCTT
GAAGGCTTGAGTTTCCCGGAAGCGGTGGAGCGGGTGGCCGATATGGCCGGTGTTCCCATGCCCGCGCGCGACCCGGAAAT
GGAAAAGCGCGAGGCGCAGCGCGCAACGCTTTACGATGTGATGGAACTGGCTGCGCAATTCTTTGAGAGCCAGTTGCAAA
GTGCGACCGGTGCGAAAGCGCGCGCCTATCTGCGCGACCGCGGCCTTTCGACCGCCACGCAGCAGGCGTTCCGCATGGGC
TATGCGCCTGAATCACGCAATGCGCTGAAGGAGTTTCTGGCAGGCAAGGGCGTTTCCCGCGAGCAGATCGAAGCCTGCGG
CCTTGTGGTGCATGGCGAGGGAATAGCGGTTTCCTACGATCGTTTCCGTGATCGCGTGATGTTCCCTATCGAGGATTTGC
GTGGGCGGATCATCGCTTTTGGCGGGCGGGCTCTTTCTGCGGATGCGCCTGCGAAATATCTCAATTCGCCCGAAACAGAG
CTCTTTCATAAGGGGCGCGTGCTTTATAACGGCTCGCGGGCGCGCAAGGCCTGCCAACCGCAGGGCGGCGAACCGGCCAA
GCCGATCATTGCCGTCGAAGGCTATATGGACGTCATCGCGCTTGCGCAGGCCGGTATCCATCATGTGGTGGCTCCGCTTG
GCACTGCTCTGACCGAAGAACAGCTTGAACTTCTCTGGCGCATCAGCCCCGAGCCGATCCTCTGTTTTGACGGTGACGGC
GCTGGCCTTCGTGCGGCCTATCGTGCTGCCGATCTTGGCCTGCCTGTCTTGCAACCCGGCAAGTCGCTGCGCTTTGCGCT
TTTGCCGGAAGGGCAGGACCCGGACGATCTCGTCAAGGCGGAAGGTCCGTCCGCTTTTCATGCGGTTCTGCGCGATGCAA
GACCGCTGGTGGAGATGGTCTGGACGCGGGAAACGGCAGGCGGGGTTTTCGATACGCCGGAGCGCCGGGCCGAACTGGAG
GCGCGTCTGCGCGAAATTACGAGCCGCATTGCCAATGAAGATATCCGCCGCCACTACAGCCAGGATATGCGCGAGCGGGC
GCAGGCCTTTTTCGGGCAGGGGCGGCAGGGCTTTCAAAACGGCGGCCAGCGCAGCCAGTCGTTTACGCGCCGCAATGAAG
GCGCGCGCGGACGCGGCGGCCCGGCCGGCAGCGGCCGGCTGGCAATCTCCGACAGTCTCGCCCGGTCGACGCTGGTAAAG
GGCGGCCGGCAGGGTGGTGCTACACATGCTCCCTTGCGCGAGACGGCCATCGTGCTGACGCTTCTCAATCATCCCCGGCT
TATCGAGGAGGATTTCGAGACGCTTGCCGCGCTTGATCTTGGCCATGATGCCTTGAAGACGCTGCATCTTGCCATGCTGG
ATGTGCTGGCGGCGGGACGTGTGGACGACGGGATCGCCATGCGGCGCGCGCTGGTGGATGCCGGTCATGGCGATCTGATC
AAGATGCTGGAGCAGGTGGTGAAGGGCGCGAAACTGTGGACCGCGACCACCCTTGCCGCTGAAGATGATGCGCGTGAAGC
ACTGCGTCAGGCGCTTCACTTGCATCAGCGCGCGCGTACACTACATAAGGAACTGCGCGCTGTTGAGGCCGCACTTGAGA
CAGATGAGACGGGCGAGTTGTTCGCGCGTCTTCGCGATATACAAAACCAGATTTTAAAAGCCGATGCGACCGAGGCCCTG
ATAGATGGATTTGGCCTGTCATCGGGGCGGCCACATGGCGGTTTCTGA

Upstream 100 bases:

>100_bases
CGGGGAGGTGCATGCGCGCGCGATGGACTGTCAAGGCGGGGACGCCTATATAGTCAATAACTATAAGCTGCCTGCTGTTC
GACCTGAAAGTGAATCTGTA

Downstream 100 bases:

>100_bases
TTCGTTTTGGCGAATCGGTTTTGCGAAAACAGCCGGAAATGCGGTCTTGCGGCCGCAAGATCGCGCCCTAACTGGGCCCT
AACTGGCTTGTGAATGGAGG

Product: DNA primase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 655; Mature: 655

Protein sequence:

>655_residues
MRFPPSFLDEIRDRVPISTLIGTRVSFDRKKSNPPKGDFWACCPFHGEKTPSFHCEDRKGRYHCFGCGVTGDHFKFLTDL
EGLSFPEAVERVADMAGVPMPARDPEMEKREAQRATLYDVMELAAQFFESQLQSATGAKARAYLRDRGLSTATQQAFRMG
YAPESRNALKEFLAGKGVSREQIEACGLVVHGEGIAVSYDRFRDRVMFPIEDLRGRIIAFGGRALSADAPAKYLNSPETE
LFHKGRVLYNGSRARKACQPQGGEPAKPIIAVEGYMDVIALAQAGIHHVVAPLGTALTEEQLELLWRISPEPILCFDGDG
AGLRAAYRAADLGLPVLQPGKSLRFALLPEGQDPDDLVKAEGPSAFHAVLRDARPLVEMVWTRETAGGVFDTPERRAELE
ARLREITSRIANEDIRRHYSQDMRERAQAFFGQGRQGFQNGGQRSQSFTRRNEGARGRGGPAGSGRLAISDSLARSTLVK
GGRQGGATHAPLRETAIVLTLLNHPRLIEEDFETLAALDLGHDALKTLHLAMLDVLAAGRVDDGIAMRRALVDAGHGDLI
KMLEQVVKGAKLWTATTLAAEDDAREALRQALHLHQRARTLHKELRAVEAALETDETGELFARLRDIQNQILKADATEAL
IDGFGLSSGRPHGGF

Sequences:

>Translated_655_residues
MRFPPSFLDEIRDRVPISTLIGTRVSFDRKKSNPPKGDFWACCPFHGEKTPSFHCEDRKGRYHCFGCGVTGDHFKFLTDL
EGLSFPEAVERVADMAGVPMPARDPEMEKREAQRATLYDVMELAAQFFESQLQSATGAKARAYLRDRGLSTATQQAFRMG
YAPESRNALKEFLAGKGVSREQIEACGLVVHGEGIAVSYDRFRDRVMFPIEDLRGRIIAFGGRALSADAPAKYLNSPETE
LFHKGRVLYNGSRARKACQPQGGEPAKPIIAVEGYMDVIALAQAGIHHVVAPLGTALTEEQLELLWRISPEPILCFDGDG
AGLRAAYRAADLGLPVLQPGKSLRFALLPEGQDPDDLVKAEGPSAFHAVLRDARPLVEMVWTRETAGGVFDTPERRAELE
ARLREITSRIANEDIRRHYSQDMRERAQAFFGQGRQGFQNGGQRSQSFTRRNEGARGRGGPAGSGRLAISDSLARSTLVK
GGRQGGATHAPLRETAIVLTLLNHPRLIEEDFETLAALDLGHDALKTLHLAMLDVLAAGRVDDGIAMRRALVDAGHGDLI
KMLEQVVKGAKLWTATTLAAEDDAREALRQALHLHQRARTLHKELRAVEAALETDETGELFARLRDIQNQILKADATEAL
IDGFGLSSGRPHGGF
>Mature_655_residues
MRFPPSFLDEIRDRVPISTLIGTRVSFDRKKSNPPKGDFWACCPFHGEKTPSFHCEDRKGRYHCFGCGVTGDHFKFLTDL
EGLSFPEAVERVADMAGVPMPARDPEMEKREAQRATLYDVMELAAQFFESQLQSATGAKARAYLRDRGLSTATQQAFRMG
YAPESRNALKEFLAGKGVSREQIEACGLVVHGEGIAVSYDRFRDRVMFPIEDLRGRIIAFGGRALSADAPAKYLNSPETE
LFHKGRVLYNGSRARKACQPQGGEPAKPIIAVEGYMDVIALAQAGIHHVVAPLGTALTEEQLELLWRISPEPILCFDGDG
AGLRAAYRAADLGLPVLQPGKSLRFALLPEGQDPDDLVKAEGPSAFHAVLRDARPLVEMVWTRETAGGVFDTPERRAELE
ARLREITSRIANEDIRRHYSQDMRERAQAFFGQGRQGFQNGGQRSQSFTRRNEGARGRGGPAGSGRLAISDSLARSTLVK
GGRQGGATHAPLRETAIVLTLLNHPRLIEEDFETLAALDLGHDALKTLHLAMLDVLAAGRVDDGIAMRRALVDAGHGDLI
KMLEQVVKGAKLWTATTLAAEDDAREALRQALHLHQRARTLHKELRAVEAALETDETGELFARLRDIQNQILKADATEAL
IDGFGLSSGRPHGGF

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis [H]

COG id: COG0358

COG function: function code L; DNA primase (bacterial type)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Escherichia coli, GI1789447, Length=425, Percent_Identity=42.5882352941177, Blast_Score=319, Evalue=3e-88,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016136
- InterPro:   IPR013264
- InterPro:   IPR019475
- InterPro:   IPR006295
- InterPro:   IPR013173
- InterPro:   IPR006171
- InterPro:   IPR002694 [H]

Pfam domain/function: PF10410 DnaB_bind; PF08278 DnaG_DnaB_bind; PF01751 Toprim; PF08275 Toprim_N; PF01807 zf-CHC2 [H]

EC number: 2.7.7.-

Molecular weight: Translated: 71936; Mature: 71936

Theoretical pI: Translated: 7.17; Mature: 7.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFPPSFLDEIRDRVPISTLIGTRVSFDRKKSNPPKGDFWACCPFHGEKTPSFHCEDRKG
CCCCHHHHHHHHHCCCHHHHHHCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCC
RYHCFGCGVTGDHFKFLTDLEGLSFPEAVERVADMAGVPMPARDPEMEKREAQRATLYDV
CEEEEECCCCCCHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHH
MELAAQFFESQLQSATGAKARAYLRDRGLSTATQQAFRMGYAPESRNALKEFLAGKGVSR
HHHHHHHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHCCCCCH
EQIEACGLVVHGEGIAVSYDRFRDRVMFPIEDLRGRIIAFGGRALSADAPAKYLNSPETE
HHHHHCCEEEECCCEEEEHHHHHCCCCCCHHHCCCCEEEECCCEECCCCCHHHHCCCHHH
LFHKGRVLYNGSRARKACQPQGGEPAKPIIAVEGYMDVIALAQAGIHHVVAPLGTALTEE
HHHCCCEEECCHHHHHHCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QLELLWRISPEPILCFDGDGAGLRAAYRAADLGLPVLQPGKSLRFALLPEGQDPDDLVKA
HHHHHHHCCCCCEEEECCCCCCHHHHHHHHHCCCCCCCCCCCCEEEECCCCCCHHHHHHC
EGPSAFHAVLRDARPLVEMVWTRETAGGVFDTPERRAELEARLREITSRIANEDIRRHYS
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QDMRERAQAFFGQGRQGFQNGGQRSQSFTRRNEGARGRGGPAGSGRLAISDSLARSTLVK
HHHHHHHHHHHHCCCHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCCEEEHHHHHHHHHHH
GGRQGGATHAPLRETAIVLTLLNHPRLIEEDFETLAALDLGHDALKTLHLAMLDVLAAGR
CCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCC
VDDGIAMRRALVDAGHGDLIKMLEQVVKGAKLWTATTLAAEDDAREALRQALHLHQRART
CCHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHEEHHCCHHHHHHHHHHHHHHHHHHH
LHKELRAVEAALETDETGELFARLRDIQNQILKADATEALIDGFGLSSGRPHGGF
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MRFPPSFLDEIRDRVPISTLIGTRVSFDRKKSNPPKGDFWACCPFHGEKTPSFHCEDRKG
CCCCHHHHHHHHHCCCHHHHHHCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCC
RYHCFGCGVTGDHFKFLTDLEGLSFPEAVERVADMAGVPMPARDPEMEKREAQRATLYDV
CEEEEECCCCCCHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHH
MELAAQFFESQLQSATGAKARAYLRDRGLSTATQQAFRMGYAPESRNALKEFLAGKGVSR
HHHHHHHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHCCCCCH
EQIEACGLVVHGEGIAVSYDRFRDRVMFPIEDLRGRIIAFGGRALSADAPAKYLNSPETE
HHHHHCCEEEECCCEEEEHHHHHCCCCCCHHHCCCCEEEECCCEECCCCCHHHHCCCHHH
LFHKGRVLYNGSRARKACQPQGGEPAKPIIAVEGYMDVIALAQAGIHHVVAPLGTALTEE
HHHCCCEEECCHHHHHHCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QLELLWRISPEPILCFDGDGAGLRAAYRAADLGLPVLQPGKSLRFALLPEGQDPDDLVKA
HHHHHHHCCCCCEEEECCCCCCHHHHHHHHHCCCCCCCCCCCCEEEECCCCCCHHHHHHC
EGPSAFHAVLRDARPLVEMVWTRETAGGVFDTPERRAELEARLREITSRIANEDIRRHYS
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QDMRERAQAFFGQGRQGFQNGGQRSQSFTRRNEGARGRGGPAGSGRLAISDSLARSTLVK
HHHHHHHHHHHHCCCHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCCEEEHHHHHHHHHHH
GGRQGGATHAPLRETAIVLTLLNHPRLIEEDFETLAALDLGHDALKTLHLAMLDVLAAGR
CCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCC
VDDGIAMRRALVDAGHGDLIKMLEQVVKGAKLWTATTLAAEDDAREALRQALHLHQRART
CCHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHEEHHCCHHHHHHHHHHHHHHHHHHH
LHKELRAVEAALETDETGELFARLRDIQNQILKADATEALIDGFGLSSGRPHGGF
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups; Nucleotidyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]