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The map label for this gene is rpoD [H]

Identifier: 23502348

GI number: 23502348

Start: 1432134

End: 1434152

Strand: Reverse

Name: rpoD [H]

Synonym: BR1479

Alternate gene names: 23502348

Gene position: 1434152-1432134 (Counterclockwise)

Preceding gene: 23502349

Following gene: 23502347

Centisome position: 68.04

GC content: 56.81

Gene sequence:

>2019_bases
ATGGCAACGAAGGTTAAGGAAAACGAAGAAGCCGAAGTCGAGCGCGAAGGTGCTGCCGACGGTCCGCTTCTCGACCTTTC
TGACGATGCTGTCAAGAAGATGATCAAGCTTGCGAAGAAGCGCGGCTACGTCACCATGGACGAGTTGAATGCCGTGCTTC
CTTCCGAGGAAGTGACTTCCGAGCAGATCGAAGACACCATGTCGATGCTTTCCGATATGGGCATCAATGTCGTTGAAGAT
GACGAGCAGGATAACGATCGCGAAGAAGGTAACGACGACGAGGCGGAAGAAGGCGGCGATCTGGTCGAAGCCGGCGGCAC
GGCTGTTGCCACCACCACGACCAAGAAAGAGCCGACCGACCGCACGGACGATCCGGTGCGCATGTATCTGCGCGAAATGG
GTTCGGTCGAGCTTTTGTCGCGCGAAGGCGAAATCGCCATTGCAAAGCGCATCGAGGCCGGGCGCGAAACCATGATCGCC
GGGCTTTGTGAAAGCCCGCTGAGCTTCCAGGCGATCATCATCTGGCGCGACCAGCTCAACGAATCCAATATTCTGCTGCG
CGAAATCATCGATCTCGAAACCACTTATGCCGGCCCCGAAGCCAAGCAGGCTCCGGTGATCGAACGCGTGGAAGAAGAAA
AGCCGCGCGATGAAAGGCCACAGCGCCGCTCGCGTGACGACGACGACATTACCAATGTCGGCGGCGATATGCCGGTGGAA
GACGATGACGACGATGAGGATGAAGCCAACCTGTCGCTGGCGGCCATGGAAGCCGAACTGCGCCCGCAGGTCATGGAAAC
GCTCGATTTCATCGCCGATACCTATAAGAAGCTGCGCAAGTTGCAGGACCAGCAGGTCGAGGGCCGCCTGGCTGCAACGG
GCGAGCTTTCCTCCAGCCAGGAGCGCCGCTACAAGGAGCTGAAAGACCAGCTTATCAAGGCTGTGAAGTCGCTTTCGCTC
AACCAGAACCGTATCGAGCAGCTTGTTGAGCAGCTTTACGATATCAATAAGCGCCTGGTGCAGAACGAAGGCAAGCTGCT
GCGTCTGGCCGAATCCTTCGGCGTGCGTCGTGAAGACTTCCTGAAGGAATATCAGGGCCATGAGCTTGACCCGAACTGGG
TGGAGCGCGTTGCTACCTTGCCAAGCCGTGGCTGGAAGGAATTTGCCGCCAAGGAAGCCGATTCCATTGGCCGTCTGCGT
AGCGAAATCCAGAATCTCGCAACCGAAACCGCTATTTCCATCGGTGAATTCCGCCGTATCGTCAATCAGGTCCAGAAGGG
CGAACGCGAAGCGACCATCGCCAAGAAGGAAATGGTGGAAGCGAACCTGCGCCTGGTGATTTCGATTGCCAAGAAGTACA
CGAACCGCGGCCTTCAGTTCCTCGATCTCATTCAGGAAGGCAATATTGGCCTCATGAAAGCGGTCGATAAGTTTGAATAT
CGCCGCGGCTACAAGTTCTCGACCTATGCGACCTGGTGGATTCGTCAGGCAATTACCCGTTCGATTGCCGATCAGGCCCG
CACGATCCGCATTCCGGTGCATATGATCGAAACGATCAACAAGATCGTTCGTACTTCGCGCCAGATGCTGCATGAAATCG
GCCGTGAACCGACGCCGGAAGAACTGGCGGAAAAGCTTGCCATGCCGCTCGAAAAAGTGCGCAAGGTGCTGAAAATCGCC
AAGGAACCGATCTCCCTCGAAACGCCGGTCGGCGATGAGGAGGATTCACACCTGGGCGATTTCATCGAGGACAAGAACGC
GCTTTTGCCGATCGATGCTGCCATTCAGGCCAATCTGCGCGATACGACGACCCGCGTTCTCGCTTCGCTGACGCCGCGTG
AGGAACGTGTTCTTCGTATGCGCTTCGGCATTGGCATGAACACTGACCATACGCTGGAAGAAGTTGGCCAGCAGTTCTCG
GTCACGCGTGAACGTATCCGCCAGATCGAGGCCAAGGCGCTGCGCAAGCTGAAGCATCCGAGCCGTTCACGCAAGCTGCG
TTCGTTCCTTGATAGCTGA

Upstream 100 bases:

>100_bases
TCGGCTACTCGCAGAATCAACTGACGGCTTGCAACCTCGCAAAACCGTTCGACCACTCTATATATTTTAGAAGCGACCGG
GGTCGCCTGGAGAAAAACAT

Downstream 100 bases:

>100_bases
ACGATACGGGAAGGGGAGGCGGTATGTCCTTTTTGAAGCGTCTTTTCGGTGGAGGCTCTGCCTCCGAAACACCGGCAGAG
CCGAAAGAGGCTGGTCGCCT

Product: RNA polymerase sigma factor RpoD

Products: NA

Alternate protein names: Sigma-A; Major vegetative sigma factor [H]

Number of amino acids: Translated: 672; Mature: 671

Protein sequence:

>672_residues
MATKVKENEEAEVEREGAADGPLLDLSDDAVKKMIKLAKKRGYVTMDELNAVLPSEEVTSEQIEDTMSMLSDMGINVVED
DEQDNDREEGNDDEAEEGGDLVEAGGTAVATTTTKKEPTDRTDDPVRMYLREMGSVELLSREGEIAIAKRIEAGRETMIA
GLCESPLSFQAIIIWRDQLNESNILLREIIDLETTYAGPEAKQAPVIERVEEEKPRDERPQRRSRDDDDITNVGGDMPVE
DDDDDEDEANLSLAAMEAELRPQVMETLDFIADTYKKLRKLQDQQVEGRLAATGELSSSQERRYKELKDQLIKAVKSLSL
NQNRIEQLVEQLYDINKRLVQNEGKLLRLAESFGVRREDFLKEYQGHELDPNWVERVATLPSRGWKEFAAKEADSIGRLR
SEIQNLATETAISIGEFRRIVNQVQKGEREATIAKKEMVEANLRLVISIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEY
RRGYKFSTYATWWIRQAITRSIADQARTIRIPVHMIETINKIVRTSRQMLHEIGREPTPEELAEKLAMPLEKVRKVLKIA
KEPISLETPVGDEEDSHLGDFIEDKNALLPIDAAIQANLRDTTTRVLASLTPREERVLRMRFGIGMNTDHTLEEVGQQFS
VTRERIRQIEAKALRKLKHPSRSRKLRSFLDS

Sequences:

>Translated_672_residues
MATKVKENEEAEVEREGAADGPLLDLSDDAVKKMIKLAKKRGYVTMDELNAVLPSEEVTSEQIEDTMSMLSDMGINVVED
DEQDNDREEGNDDEAEEGGDLVEAGGTAVATTTTKKEPTDRTDDPVRMYLREMGSVELLSREGEIAIAKRIEAGRETMIA
GLCESPLSFQAIIIWRDQLNESNILLREIIDLETTYAGPEAKQAPVIERVEEEKPRDERPQRRSRDDDDITNVGGDMPVE
DDDDDEDEANLSLAAMEAELRPQVMETLDFIADTYKKLRKLQDQQVEGRLAATGELSSSQERRYKELKDQLIKAVKSLSL
NQNRIEQLVEQLYDINKRLVQNEGKLLRLAESFGVRREDFLKEYQGHELDPNWVERVATLPSRGWKEFAAKEADSIGRLR
SEIQNLATETAISIGEFRRIVNQVQKGEREATIAKKEMVEANLRLVISIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEY
RRGYKFSTYATWWIRQAITRSIADQARTIRIPVHMIETINKIVRTSRQMLHEIGREPTPEELAEKLAMPLEKVRKVLKIA
KEPISLETPVGDEEDSHLGDFIEDKNALLPIDAAIQANLRDTTTRVLASLTPREERVLRMRFGIGMNTDHTLEEVGQQFS
VTRERIRQIEAKALRKLKHPSRSRKLRSFLDS
>Mature_671_residues
ATKVKENEEAEVEREGAADGPLLDLSDDAVKKMIKLAKKRGYVTMDELNAVLPSEEVTSEQIEDTMSMLSDMGINVVEDD
EQDNDREEGNDDEAEEGGDLVEAGGTAVATTTTKKEPTDRTDDPVRMYLREMGSVELLSREGEIAIAKRIEAGRETMIAG
LCESPLSFQAIIIWRDQLNESNILLREIIDLETTYAGPEAKQAPVIERVEEEKPRDERPQRRSRDDDDITNVGGDMPVED
DDDDEDEANLSLAAMEAELRPQVMETLDFIADTYKKLRKLQDQQVEGRLAATGELSSSQERRYKELKDQLIKAVKSLSLN
QNRIEQLVEQLYDINKRLVQNEGKLLRLAESFGVRREDFLKEYQGHELDPNWVERVATLPSRGWKEFAAKEADSIGRLRS
EIQNLATETAISIGEFRRIVNQVQKGEREATIAKKEMVEANLRLVISIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYR
RGYKFSTYATWWIRQAITRSIADQARTIRIPVHMIETINKIVRTSRQMLHEIGREPTPEELAEKLAMPLEKVRKVLKIAK
EPISLETPVGDEEDSHLGDFIEDKNALLPIDAAIQANLRDTTTRVLASLTPREERVLRMRFGIGMNTDHTLEEVGQQFSV
TRERIRQIEAKALRKLKHPSRSRKLRSFLDS

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This is the primary sigma factor of this bacterium [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789448, Length=652, Percent_Identity=48.3128834355828, Blast_Score=509, Evalue=1e-145,
Organism=Escherichia coli, GI1789098, Length=225, Percent_Identity=42.6666666666667, Blast_Score=211, Evalue=1e-55,
Organism=Escherichia coli, GI1789871, Length=280, Percent_Identity=26.7857142857143, Blast_Score=86, Evalue=1e-17,
Organism=Escherichia coli, GI1788231, Length=202, Percent_Identity=27.7227722772277, Blast_Score=69, Evalue=1e-12,

Paralogues:

None

Copy number: 700 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR007631
- InterPro:   IPR007127
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012760
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04546 Sigma70_ner; PF03979 Sigma70_r1_1; PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 76533; Mature: 76401

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATKVKENEEAEVEREGAADGPLLDLSDDAVKKMIKLAKKRGYVTMDELNAVLPSEEVTS
CCCCCCCCCHHHHHHCCCCCCCEECCCHHHHHHHHHHHHHCCCEEHHHHHHHCCCHHHHH
EQIEDTMSMLSDMGINVVEDDEQDNDREEGNDDEAEEGGDLVEAGGTAVATTTTKKEPTD
HHHHHHHHHHHHCCCCEECCCCCCCCCCCCCCCCHHHCCCEECCCCCEEEEECCCCCCCC
RTDDPVRMYLREMGSVELLSREGEIAIAKRIEAGRETMIAGLCESPLSFQAIIIWRDQLN
CCCHHHHHHHHHCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCC
ESNILLREIIDLETTYAGPEAKQAPVIERVEEEKPRDERPQRRSRDDDDITNVGGDMPVE
CCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCHHHCCCCCCHHHHCCCCCCCC
DDDDDEDEANLSLAAMEAELRPQVMETLDFIADTYKKLRKLQDQQVEGRLAATGELSSSQ
CCCCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCHH
ERRYKELKDQLIKAVKSLSLNQNRIEQLVEQLYDINKRLVQNEGKLLRLAESFGVRREDF
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHH
LKEYQGHELDPNWVERVATLPSRGWKEFAAKEADSIGRLRSEIQNLATETAISIGEFRRI
HHHHCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VNQVQKGEREATIAKKEMVEANLRLVISIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEY
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHH
RRGYKFSTYATWWIRQAITRSIADQARTIRIPVHMIETINKIVRTSRQMLHEIGREPTPE
HCCCCCHHHHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
ELAEKLAMPLEKVRKVLKIAKEPISLETPVGDEEDSHLGDFIEDKNALLPIDAAIQANLR
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHCCCCEEEEHHHHHCCCH
DTTTRVLASLTPREERVLRMRFGIGMNTDHTLEEVGQQFSVTRERIRQIEAKALRKLKHP
HHHHHHHHHCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
SRSRKLRSFLDS
HHHHHHHHHHCC
>Mature Secondary Structure 
ATKVKENEEAEVEREGAADGPLLDLSDDAVKKMIKLAKKRGYVTMDELNAVLPSEEVTS
CCCCCCCCHHHHHHCCCCCCCEECCCHHHHHHHHHHHHHCCCEEHHHHHHHCCCHHHHH
EQIEDTMSMLSDMGINVVEDDEQDNDREEGNDDEAEEGGDLVEAGGTAVATTTTKKEPTD
HHHHHHHHHHHHCCCCEECCCCCCCCCCCCCCCCHHHCCCEECCCCCEEEEECCCCCCCC
RTDDPVRMYLREMGSVELLSREGEIAIAKRIEAGRETMIAGLCESPLSFQAIIIWRDQLN
CCCHHHHHHHHHCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCC
ESNILLREIIDLETTYAGPEAKQAPVIERVEEEKPRDERPQRRSRDDDDITNVGGDMPVE
CCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCHHHCCCCCCHHHHCCCCCCCC
DDDDDEDEANLSLAAMEAELRPQVMETLDFIADTYKKLRKLQDQQVEGRLAATGELSSSQ
CCCCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCHH
ERRYKELKDQLIKAVKSLSLNQNRIEQLVEQLYDINKRLVQNEGKLLRLAESFGVRREDF
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHH
LKEYQGHELDPNWVERVATLPSRGWKEFAAKEADSIGRLRSEIQNLATETAISIGEFRRI
HHHHCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VNQVQKGEREATIAKKEMVEANLRLVISIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEY
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHH
RRGYKFSTYATWWIRQAITRSIADQARTIRIPVHMIETINKIVRTSRQMLHEIGREPTPE
HCCCCCHHHHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
ELAEKLAMPLEKVRKVLKIAKEPISLETPVGDEEDSHLGDFIEDKNALLPIDAAIQANLR
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHCCCCEEEEHHHHHCCCH
DTTTRVLASLTPREERVLRMRFGIGMNTDHTLEEVGQQFSVTRERIRQIEAKALRKLKHP
HHHHHHHHHCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
SRSRKLRSFLDS
HHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8491721; 11743193; 11743194 [H]