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The map label for this gene is ddl [H]

Identifier: 23502299

GI number: 23502299

Start: 1384478

End: 1385404

Strand: Reverse

Name: ddl [H]

Synonym: BR1428

Alternate gene names: 23502299

Gene position: 1385404-1384478 (Counterclockwise)

Preceding gene: 23502300

Following gene: 23502298

Centisome position: 65.73

GC content: 55.45

Gene sequence:

>927_bases
ATGACGGGAAAACACGTCGCCGTTTTGATGGGTGGTTTTTCTTCGGAACGCTCCGTCAGCCTTTCCTCCGGTGTGGCCTG
TGCAACGACGCTTGAAGAATGCGGCTATCGCGTCACGCGCATTGATGTGGATCGCAATGTTGCGAGCATCCTGGTCGAGT
TGAAACCCGATGTTGTGTTCAACGCCTTGCACGGCCCCTTCGGCGAAGATGGCGCCATTCAGGGCGTGCTTGAATATCTC
CAGATTCCCTACACACATTCCGGCGTGCTGGCTTCCGCGCTTGCGATGGACAAGGATCGCGCCAAGAAGGTGGCGGCAGC
GGCGGGCGTCGTGGTTGCGCCGTCACGCCTGATGAACCGTTTCGACATTGGTTCGCAGCATCCGATGAAGCCGCCTTACG
TGGTGAAGCCGGTGCGTGAAGGGTCGAGCTTCGGTGTGGTGATCGTCAAGGAAGATCAGCCGCATCCGCCGCAGGTCATC
GGTTCGGCCGATTGGAAATATGGTGATGAGGTCATGGTCGAAGGCTATATTGCCGGGCGTGAGCTGACCTGTGCCGTGAT
GGGGGATCGCGCCATGGATGTGTGCGAGATCATTCCGGTCGGTTATCAGTTCTACGACTATGATTCAAAATATGTTGCAG
GTGCATCAACTCACGTGTCCCCGGCAAAAATTTTACCAAATATTTACCAAAAAATACAAACAATGGCCCTTACGGCGCAT
CGGGCAATCGGGTGTCGGGGCGTAAGCCGGTCGGACTTCCGTTTCGATGATCGTTTTTCTGAAGAAGGCGAAGTTGTCTG
GCTGGAAATCAATACCCAGCCGGGGATGACTCCTACCTCGCTCGTGCCTGATATCGCCAAAGCGGCGGGTATCTCTTTCG
CTGAGTTGTTGAGTTGGATGGTGGAGGACGCGTCTTGTTTGCGTTGA

Upstream 100 bases:

>100_bases
GGCAGGATTTTCCTGCTGTGGGTGCCTGCGGCTTGCGTCGCCGGGTTATCCGGACTCTGTTCTTGATTTATGCGCAGGAA
TGCGCAGAGGGGAAGCTAGA

Downstream 100 bases:

>100_bases
ACGGAAAATCGGATGGATATAGGCGCGCGGGCGCGGTGCGGGATGCATCGGGCGCGATGAATGCCGCTTTTGTCCTGCCG
CGCTTCCTGCGCAAGCCGTT

Product: D-alanine--D-alanine ligase

Products: NA

Alternate protein names: D-Ala-D-Ala ligase B; D-alanylalanine synthetase B [H]

Number of amino acids: Translated: 308; Mature: 307

Protein sequence:

>308_residues
MTGKHVAVLMGGFSSERSVSLSSGVACATTLEECGYRVTRIDVDRNVASILVELKPDVVFNALHGPFGEDGAIQGVLEYL
QIPYTHSGVLASALAMDKDRAKKVAAAAGVVVAPSRLMNRFDIGSQHPMKPPYVVKPVREGSSFGVVIVKEDQPHPPQVI
GSADWKYGDEVMVEGYIAGRELTCAVMGDRAMDVCEIIPVGYQFYDYDSKYVAGASTHVSPAKILPNIYQKIQTMALTAH
RAIGCRGVSRSDFRFDDRFSEEGEVVWLEINTQPGMTPTSLVPDIAKAAGISFAELLSWMVEDASCLR

Sequences:

>Translated_308_residues
MTGKHVAVLMGGFSSERSVSLSSGVACATTLEECGYRVTRIDVDRNVASILVELKPDVVFNALHGPFGEDGAIQGVLEYL
QIPYTHSGVLASALAMDKDRAKKVAAAAGVVVAPSRLMNRFDIGSQHPMKPPYVVKPVREGSSFGVVIVKEDQPHPPQVI
GSADWKYGDEVMVEGYIAGRELTCAVMGDRAMDVCEIIPVGYQFYDYDSKYVAGASTHVSPAKILPNIYQKIQTMALTAH
RAIGCRGVSRSDFRFDDRFSEEGEVVWLEINTQPGMTPTSLVPDIAKAAGISFAELLSWMVEDASCLR
>Mature_307_residues
TGKHVAVLMGGFSSERSVSLSSGVACATTLEECGYRVTRIDVDRNVASILVELKPDVVFNALHGPFGEDGAIQGVLEYLQ
IPYTHSGVLASALAMDKDRAKKVAAAAGVVVAPSRLMNRFDIGSQHPMKPPYVVKPVREGSSFGVVIVKEDQPHPPQVIG
SADWKYGDEVMVEGYIAGRELTCAVMGDRAMDVCEIIPVGYQFYDYDSKYVAGASTHVSPAKILPNIYQKIQTMALTAHR
AIGCRGVSRSDFRFDDRFSEEGEVVWLEINTQPGMTPTSLVPDIAKAAGISFAELLSWMVEDASCLR

Specific function: Cell wall formation [H]

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1786280, Length=314, Percent_Identity=38.2165605095541, Blast_Score=186, Evalue=1e-48,
Organism=Escherichia coli, GI1786579, Length=256, Percent_Identity=37.109375, Blast_Score=152, Evalue=2e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR000291
- InterPro:   IPR005905
- InterPro:   IPR011095
- InterPro:   IPR011127
- InterPro:   IPR013817
- InterPro:   IPR016185 [H]

Pfam domain/function: PF07478 Dala_Dala_lig_C; PF01820 Dala_Dala_lig_N [H]

EC number: =6.3.2.4 [H]

Molecular weight: Translated: 33379; Mature: 33247

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: PS50975 ATP_GRASP ; PS00843 DALA_DALA_LIGASE_1 ; PS00844 DALA_DALA_LIGASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGKHVAVLMGGFSSERSVSLSSGVACATTLEECGYRVTRIDVDRNVASILVELKPDVVF
CCCCEEEEEEECCCCCCCEECCCCCHHHHHHHHHCCEEEEEECCCCHHHHEEECCCCCEE
NALHGPFGEDGAIQGVLEYLQIPYTHSGVLASALAMDKDRAKKVAAAAGVVVAPSRLMNR
EHHCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHCHHHHHHHHHHCCEEECHHHHHHH
FDIGSQHPMKPPYVVKPVREGSSFGVVIVKEDQPHPPQVIGSADWKYGDEVMVEGYIAGR
HCCCCCCCCCCCEEEEECCCCCCEEEEEEECCCCCCCCEECCCCCCCCCEEEEEEEECCC
ELTCAVMGDRAMDVCEIIPVGYQFYDYDSKYVAGASTHVSPAKILPNIYQKIQTMALTAH
EEEEEEECCCHHHHHHHEECCEEEEECCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHH
RAIGCRGVSRSDFRFDDRFSEEGEVVWLEINTQPGMTPTSLVPDIAKAAGISFAELLSWM
HHHCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCHHHHHHHH
VEDASCLR
HHHHHHCC
>Mature Secondary Structure 
TGKHVAVLMGGFSSERSVSLSSGVACATTLEECGYRVTRIDVDRNVASILVELKPDVVF
CCCEEEEEEECCCCCCCEECCCCCHHHHHHHHHCCEEEEEECCCCHHHHEEECCCCCEE
NALHGPFGEDGAIQGVLEYLQIPYTHSGVLASALAMDKDRAKKVAAAAGVVVAPSRLMNR
EHHCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHCHHHHHHHHHHCCEEECHHHHHHH
FDIGSQHPMKPPYVVKPVREGSSFGVVIVKEDQPHPPQVIGSADWKYGDEVMVEGYIAGR
HCCCCCCCCCCCEEEEECCCCCCEEEEEEECCCCCCCCEECCCCCCCCCEEEEEEEECCC
ELTCAVMGDRAMDVCEIIPVGYQFYDYDSKYVAGASTHVSPAKILPNIYQKIQTMALTAH
EEEEEEECCCHHHHHHHEECCEEEEECCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHH
RAIGCRGVSRSDFRFDDRFSEEGEVVWLEINTQPGMTPTSLVPDIAKAAGISFAELLSWM
HHHCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCHHHHHHHH
VEDASCLR
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11756688 [H]