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The map label for this gene is btuB [H]

Identifier: 23502219

GI number: 23502219

Start: 1307049

End: 1308911

Strand: Reverse

Name: btuB [H]

Synonym: BR1347

Alternate gene names: 23502219

Gene position: 1308911-1307049 (Counterclockwise)

Preceding gene: 23502220

Following gene: 23502218

Centisome position: 62.1

GC content: 56.47

Gene sequence:

>1863_bases
ATGCATATTCTGAAATTGTCAGCTCTTCTGGCGTCGGCCTGTGCGGCCACGTTATCACCCGCTTTGGCGCAGGATGGCGG
GGATAAGGATGATGGTGTCACACTTGATACTATCGTGGTGACACCGCTCCGCCGGGCCTCGTCGCTTCAGCGATCCACGT
CCTCGGTGAGCGTTATTGACGCTGCCGATATCGAACGGTCCGCCGCGCCCGATCTGCAATCCTTGCTGCAAACCTATAGC
GGCATTTCGGTCAAGACGAATGGCGGGCAGGGGTCTTCCGCCAATATCTATATGCGCGGCATGTCGTCGAAGCAGACGGT
CGTGCTGGTCAATGGGGTGCGCACGGCTTCGGCAACGAGTGGATCGACCGCGCTTGCCAATATTCCGCTGACCTCCATCG
AACGTATCGAGATTGCCAGGGGCGCGCATTCCTCGCAATATGGCGCGGATGCAATCGGCGGTGTCATCAATATCATCACC
AAACAGGGCGGGGCCTGCGGCGAACGCGCATGGTGCGGCAGCGTTTCAACAGGCGTGTCGCATCCATGGGGCGGTTATGC
ATCGGGTTCGTTACAAGGCCGCAGCAGCGACGGTATCGATTATGCCGTAGGGGCAGCGTTTACCGGCACTCAAGGCTATG
ATTTCACCACGCCGGAAGCATTCGGCCACGAGCCGGACGATGATGGTTTCCTGCAGGGCTCGTTCAATTTTGCGCTGTCG
AAAGATTTCGACTGGGGCAAAATCTATGCGGACGGCCTCTTCAGCCGTGGGCGCAACCAGTATGATGCGACCGCACCCGC
ATTCAACGAAGCGGATAGTACGGCCTTTACCGGCAAGGTCGGCACACGGATCGACCATACGGCCGACTGGTCCTCGACGG
TGGAATTCAGCACCGGGATCGACAATAGCCGGAATTTCCGCAAGGGGATCGAAGGTTCGGACCGGTTCGAGACCAGGCGT
TACGGGGTGTTCGCCTCGACCGAAAAAAGCTTCGATACCGGCAAGGTCTCGCATGTTGTGACTGGCGGCGTTGAAGCCTA
TCGGGAAAAAATCAATACGACCATCGATTATGACGAGACAGGCCGCGATCTTGCCGCCGTGTTCGGGCAATATTCGCTGG
AATATGATGCATTGCGTTTCGATGGCGGCATTCGCTACGACCATAATGGCCAGTTCGGCAATGTGACCACCTATAATCTT
GGCGCGAGCTATGAAATCCTGCCTGATCTGGTGCTGCGTTCATCCTATGCGACGGGTTTCCGCGCCCCGACTTTCAACGA
GCTTTATTATCCGGGCTTTGCCAATCCCGACCTGCAACCCGAAAAATCCCGTTCTGTGGAAGTCGGGCTGAACTGGCAGG
CGACGGCTTCGACAAGCCTCGACATGGCACTCTATCAGACCCGGCTCAGCGATGCGATCATGAGCACTGCGCCGTCCTAT
ATTCCGTATAATATTGCAAGCGCGAAGGTGACCGGCCTTGAAGCGACACTCAGCCATCGCTTCAACGAACAATGGGGCAT
CAAGGGAATGGTGGACCTCAAGCGGCCTGTCGATGAAGATAGCGGGAATGATCTTCCCTATCGCGAGCGTTTCAAGGCGG
CCGCCGAAGTGAACTTCAAGCCGGTGGAAAAGCTCGATCTGACGGCACGGGTGCTCTATGGCGGTTCGCGCTATACCAAT
GCGAAAAATACGAAGAAACTCGGCGATTATGTCACCGCGGATTTTGTGGCGCTCTATTCGATCGACAAACAATCGCAGTT
GAAGTTCTCGGTGGAGAATATCTTCGACAAGGATTACGAGACGAGTTCCGGCTATGTTGCGCCGGGCCGCACCATCACTA
TCGGGCTGACCCGTAATTTCTGA

Upstream 100 bases:

>100_bases
TTGCCGTCAGGAAAGAAACAGCTTTGCGCTGGCACTTCTTCAACCTGCGGTGAGAGGGAAGCTGGACAGGGAAAACCCGT
CCGGCAGGTTGAGGGATAAA

Downstream 100 bases:

>100_bases
GATTGGACAGGATGTGAAAAGGGTTTCCGTAAAATGGACGAGGAGGCTCTGCCTTGCGGCAGGTCTGGCCCTTTTTGCCC
CCCTGCCGGCCAATGCGGCT

Product: iron compound TonB-dependent receptor

Products: NA

Alternate protein names: Cobalamin receptor; Outer membrane cobalamin translocator [H]

Number of amino acids: Translated: 620; Mature: 620

Protein sequence:

>620_residues
MHILKLSALLASACAATLSPALAQDGGDKDDGVTLDTIVVTPLRRASSLQRSTSSVSVIDAADIERSAAPDLQSLLQTYS
GISVKTNGGQGSSANIYMRGMSSKQTVVLVNGVRTASATSGSTALANIPLTSIERIEIARGAHSSQYGADAIGGVINIIT
KQGGACGERAWCGSVSTGVSHPWGGYASGSLQGRSSDGIDYAVGAAFTGTQGYDFTTPEAFGHEPDDDGFLQGSFNFALS
KDFDWGKIYADGLFSRGRNQYDATAPAFNEADSTAFTGKVGTRIDHTADWSSTVEFSTGIDNSRNFRKGIEGSDRFETRR
YGVFASTEKSFDTGKVSHVVTGGVEAYREKINTTIDYDETGRDLAAVFGQYSLEYDALRFDGGIRYDHNGQFGNVTTYNL
GASYEILPDLVLRSSYATGFRAPTFNELYYPGFANPDLQPEKSRSVEVGLNWQATASTSLDMALYQTRLSDAIMSTAPSY
IPYNIASAKVTGLEATLSHRFNEQWGIKGMVDLKRPVDEDSGNDLPYRERFKAAAEVNFKPVEKLDLTARVLYGGSRYTN
AKNTKKLGDYVTADFVALYSIDKQSQLKFSVENIFDKDYETSSGYVAPGRTITIGLTRNF

Sequences:

>Translated_620_residues
MHILKLSALLASACAATLSPALAQDGGDKDDGVTLDTIVVTPLRRASSLQRSTSSVSVIDAADIERSAAPDLQSLLQTYS
GISVKTNGGQGSSANIYMRGMSSKQTVVLVNGVRTASATSGSTALANIPLTSIERIEIARGAHSSQYGADAIGGVINIIT
KQGGACGERAWCGSVSTGVSHPWGGYASGSLQGRSSDGIDYAVGAAFTGTQGYDFTTPEAFGHEPDDDGFLQGSFNFALS
KDFDWGKIYADGLFSRGRNQYDATAPAFNEADSTAFTGKVGTRIDHTADWSSTVEFSTGIDNSRNFRKGIEGSDRFETRR
YGVFASTEKSFDTGKVSHVVTGGVEAYREKINTTIDYDETGRDLAAVFGQYSLEYDALRFDGGIRYDHNGQFGNVTTYNL
GASYEILPDLVLRSSYATGFRAPTFNELYYPGFANPDLQPEKSRSVEVGLNWQATASTSLDMALYQTRLSDAIMSTAPSY
IPYNIASAKVTGLEATLSHRFNEQWGIKGMVDLKRPVDEDSGNDLPYRERFKAAAEVNFKPVEKLDLTARVLYGGSRYTN
AKNTKKLGDYVTADFVALYSIDKQSQLKFSVENIFDKDYETSSGYVAPGRTITIGLTRNF
>Mature_620_residues
MHILKLSALLASACAATLSPALAQDGGDKDDGVTLDTIVVTPLRRASSLQRSTSSVSVIDAADIERSAAPDLQSLLQTYS
GISVKTNGGQGSSANIYMRGMSSKQTVVLVNGVRTASATSGSTALANIPLTSIERIEIARGAHSSQYGADAIGGVINIIT
KQGGACGERAWCGSVSTGVSHPWGGYASGSLQGRSSDGIDYAVGAAFTGTQGYDFTTPEAFGHEPDDDGFLQGSFNFALS
KDFDWGKIYADGLFSRGRNQYDATAPAFNEADSTAFTGKVGTRIDHTADWSSTVEFSTGIDNSRNFRKGIEGSDRFETRR
YGVFASTEKSFDTGKVSHVVTGGVEAYREKINTTIDYDETGRDLAAVFGQYSLEYDALRFDGGIRYDHNGQFGNVTTYNL
GASYEILPDLVLRSSYATGFRAPTFNELYYPGFANPDLQPEKSRSVEVGLNWQATASTSLDMALYQTRLSDAIMSTAPSY
IPYNIASAKVTGLEATLSHRFNEQWGIKGMVDLKRPVDEDSGNDLPYRERFKAAAEVNFKPVEKLDLTARVLYGGSRYTN
AKNTKKLGDYVTADFVALYSIDKQSQLKFSVENIFDKDYETSSGYVAPGRTITIGLTRNF

Specific function: Involved in the active translocation of vitamin B12 (cyanocobalamin) across the outer membrane to the periplasmic space. It derives its energy for transport by interacting with the trans-periplasmic membrane protein TonB [H]

COG id: COG4206

COG function: function code H; Outer membrane cobalamin receptor protein

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TonB-dependent receptor family. BtuB (TC 1.B.14.3.1) subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790405, Length=664, Percent_Identity=26.5060240963855, Blast_Score=175, Evalue=6e-45,
Organism=Escherichia coli, GI1788478, Length=173, Percent_Identity=37.5722543352601, Blast_Score=97, Evalue=4e-21,
Organism=Escherichia coli, GI1786798, Length=152, Percent_Identity=33.5526315789474, Blast_Score=73, Evalue=5e-14,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012910
- InterPro:   IPR000531 [H]

Pfam domain/function: PF07715 Plug; PF00593 TonB_dep_Rec [H]

EC number: NA

Molecular weight: Translated: 66892; Mature: 66892

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHILKLSALLASACAATLSPALAQDGGDKDDGVTLDTIVVTPLRRASSLQRSTSSVSVID
CCEEHHHHHHHHHHHHHHCHHHHHCCCCCCCCEEEEEEEECCCHHHHHHHHCCCCEEEEE
AADIERSAAPDLQSLLQTYSGISVKTNGGQGSSANIYMRGMSSKQTVVLVNGVRTASATS
HHHCCCCCCHHHHHHHHHHCCCEEEECCCCCCCCEEEEEECCCCCEEEEEECCEECCCCC
GSTALANIPLTSIERIEIARGAHSSQYGADAIGGVINIITKQGGACGERAWCGSVSTGVS
CCCEEEECCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
HPWGGYASGSLQGRSSDGIDYAVGAAFTGTQGYDFTTPEAFGHEPDDDGFLQGSFNFALS
CCCCCCCCCCCCCCCCCCCCEEECCEEECCCCCCCCCCHHCCCCCCCCCEEEEEEEEEEE
KDFDWGKIYADGLFSRGRNQYDATAPAFNEADSTAFTGKVGTRIDHTADWSSTVEFSTGI
CCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCEEEEECCC
DNSRNFRKGIEGSDRFETRRYGVFASTEKSFDTGKVSHVVTGGVEAYREKINTTIDYDET
CCCCHHHHCCCCCCCCHHHHCCEEECCCCCCCCCCCCEEEECCHHHHHHHHCCEECCCCC
GRDLAAVFGQYSLEYDALRFDGGIRYDHNGQFGNVTTYNLGASYEILPDLVLRSSYATGF
CHHHHHHHHCCCCEEEEEEECCCEEECCCCCCCCEEEEECCCCHHHHHHHHHHHCCCCCC
RAPTFNELYYPGFANPDLQPEKSRSVEVGLNWQATASTSLDMALYQTRLSDAIMSTAPSY
CCCCCCCEECCCCCCCCCCCCCCCCEEEECCEEECCCCCHHHHHHHHHHHHHHHHCCCCC
IPYNIASAKVTGLEATLSHRFNEQWGIKGMVDLKRPVDEDSGNDLPYRERFKAAAEVNFK
CCEECCCEEEECHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHCCCCC
PVEKLDLTARVLYGGSRYTNAKNTKKLGDYVTADFVALYSIDKQSQLKFSVENIFDKDYE
CCHHHCCEEEEEECCCEECCCCCHHHHHHHHHHHEEEEEECCCCHHHEEEHHHHCCCCCC
TSSGYVAPGRTITIGLTRNF
CCCCEECCCCEEEEEEEECC
>Mature Secondary Structure
MHILKLSALLASACAATLSPALAQDGGDKDDGVTLDTIVVTPLRRASSLQRSTSSVSVID
CCEEHHHHHHHHHHHHHHCHHHHHCCCCCCCCEEEEEEEECCCHHHHHHHHCCCCEEEEE
AADIERSAAPDLQSLLQTYSGISVKTNGGQGSSANIYMRGMSSKQTVVLVNGVRTASATS
HHHCCCCCCHHHHHHHHHHCCCEEEECCCCCCCCEEEEEECCCCCEEEEEECCEECCCCC
GSTALANIPLTSIERIEIARGAHSSQYGADAIGGVINIITKQGGACGERAWCGSVSTGVS
CCCEEEECCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
HPWGGYASGSLQGRSSDGIDYAVGAAFTGTQGYDFTTPEAFGHEPDDDGFLQGSFNFALS
CCCCCCCCCCCCCCCCCCCCEEECCEEECCCCCCCCCCHHCCCCCCCCCEEEEEEEEEEE
KDFDWGKIYADGLFSRGRNQYDATAPAFNEADSTAFTGKVGTRIDHTADWSSTVEFSTGI
CCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCEEEEECCC
DNSRNFRKGIEGSDRFETRRYGVFASTEKSFDTGKVSHVVTGGVEAYREKINTTIDYDET
CCCCHHHHCCCCCCCCHHHHCCEEECCCCCCCCCCCCEEEECCHHHHHHHHCCEECCCCC
GRDLAAVFGQYSLEYDALRFDGGIRYDHNGQFGNVTTYNLGASYEILPDLVLRSSYATGF
CHHHHHHHHCCCCEEEEEEECCCEEECCCCCCCCEEEEECCCCHHHHHHHHHHHCCCCCC
RAPTFNELYYPGFANPDLQPEKSRSVEVGLNWQATASTSLDMALYQTRLSDAIMSTAPSY
CCCCCCCEECCCCCCCCCCCCCCCCEEEECCEEECCCCCHHHHHHHHHHHHHHHHCCCCC
IPYNIASAKVTGLEATLSHRFNEQWGIKGMVDLKRPVDEDSGNDLPYRERFKAAAEVNFK
CCEECCCEEEECHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHCCCCC
PVEKLDLTARVLYGGSRYTNAKNTKKLGDYVTADFVALYSIDKQSQLKFSVENIFDKDYE
CCHHHCCEEEEEECCCEECCCCCHHHHHHHHHHHEEEEEECCCCHHHEEEHHHHCCCCCC
TSSGYVAPGRTITIGLTRNF
CCCCEECCCCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA