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The map label for this gene is trmJ [H]

Identifier: 23502073

GI number: 23502073

Start: 1171860

End: 1172690

Strand: Reverse

Name: trmJ [H]

Synonym: BR1196

Alternate gene names: 23502073

Gene position: 1172690-1171860 (Counterclockwise)

Preceding gene: 23502078

Following gene: 23502072

Centisome position: 55.64

GC content: 57.52

Gene sequence:

>831_bases
ATGGCAGGAACAGACAAACAGCGCCCCATCCTTGCGGAAGGACCGGCAATCATTCTGGTCAATCCACAATTGCCGGAAAA
TATCGGCATGGTGGCGCGCGCCATGGCCAATTTCGGCCTGGCGGAACTGCGTCTCGTCAATCCGCGCGAAGAATTTCCAA
GCGAAAAGGCAAGGGCAGCGGCCAGTAAGGCCGATCATGTGATCGATAATGCCGTCGTTTACGACGATCTGCCTTCCGCA
ATTGCCGACCTCAATTTCGTCTATGCAACCACGGCGCGCGAGCGCGACGGCTTCAAGCCGGTGCGCAGCGCCATGGAAGC
GGGCAATGCGTTGCGCCGCCGTTTCAAAACCGGCGAGAAAACGGGCATCCTGTTCGGGCGTGAGCGTTTTGGCCTCAGCA
ATGAGGAAGTGGGCCTTGCGGATGAACTGTTGACTTTCCCGGTCAATCCGGCTTTCGCCTCGCTCAATATTGCGCAGGCC
GTTTTGCTCATGTCCTATGAATGGATGAAGTCGGGCTTGGAAAACCAGACGGATACGGTTTTCCGTGGGCCGGAAATGCC
GCTGGCGCCAAAGGAAGAGCTGCACGGGTTTTTCAACCATCTGGAAGAAGCACTGGATGTTCGGGGATATTTTCGCCCTG
AAGCGCGCAAGGAAATCATGGTCAACAATCTGCGCGCCGTATTGACGCGCGCGGGGTTTGCCTCTGCGGAACTGAAGCTC
TTGCGAGGCGTTGTTACCTCGCTCGACGTCTTTACGCCGAAGAAGCCGCGCGGCTCCGGTGCACCGGAAGGACGCGCGCG
TAAACCTGTCAATGAGGACAAGGCAGAATGA

Upstream 100 bases:

>100_bases
TACACAGGCTTTTGCTGAAACAGGTAAGGGGAATCTTTGCGATTCCGCGTCAATTATGTCAGGGAAACGCCACGATTTCA
TAGCTAATGCGGAAAACATC

Downstream 100 bases:

>100_bases
AAAAAGCACCTGCCGGCAGTTTTCCCGCGAAACCGACCATCGCTCCAGAAAGGCCAATTCTGGTCTTCGATAGCGGTATT
GGCGGCTTGACGGTCTTGCG

Product: RNA methyltransferase

Products: NA

Alternate protein names: tRNA Cm32/Um32 methyltransferase [H]

Number of amino acids: Translated: 276; Mature: 275

Protein sequence:

>276_residues
MAGTDKQRPILAEGPAIILVNPQLPENIGMVARAMANFGLAELRLVNPREEFPSEKARAAASKADHVIDNAVVYDDLPSA
IADLNFVYATTARERDGFKPVRSAMEAGNALRRRFKTGEKTGILFGRERFGLSNEEVGLADELLTFPVNPAFASLNIAQA
VLLMSYEWMKSGLENQTDTVFRGPEMPLAPKEELHGFFNHLEEALDVRGYFRPEARKEIMVNNLRAVLTRAGFASAELKL
LRGVVTSLDVFTPKKPRGSGAPEGRARKPVNEDKAE

Sequences:

>Translated_276_residues
MAGTDKQRPILAEGPAIILVNPQLPENIGMVARAMANFGLAELRLVNPREEFPSEKARAAASKADHVIDNAVVYDDLPSA
IADLNFVYATTARERDGFKPVRSAMEAGNALRRRFKTGEKTGILFGRERFGLSNEEVGLADELLTFPVNPAFASLNIAQA
VLLMSYEWMKSGLENQTDTVFRGPEMPLAPKEELHGFFNHLEEALDVRGYFRPEARKEIMVNNLRAVLTRAGFASAELKL
LRGVVTSLDVFTPKKPRGSGAPEGRARKPVNEDKAE
>Mature_275_residues
AGTDKQRPILAEGPAIILVNPQLPENIGMVARAMANFGLAELRLVNPREEFPSEKARAAASKADHVIDNAVVYDDLPSAI
ADLNFVYATTARERDGFKPVRSAMEAGNALRRRFKTGEKTGILFGRERFGLSNEEVGLADELLTFPVNPAFASLNIAQAV
LLMSYEWMKSGLENQTDTVFRGPEMPLAPKEELHGFFNHLEEALDVRGYFRPEARKEIMVNNLRAVLTRAGFASAELKLL
RGVVTSLDVFTPKKPRGSGAPEGRARKPVNEDKAE

Specific function: Catalyzes the formation of 2'O-methylated cytidine (Cm32) or 2'O-methylated uridine (Um32) at position 32 in tRNA [H]

COG id: COG0565

COG function: function code J; rRNA methylase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1788881, Length=235, Percent_Identity=36.1702127659575, Blast_Score=123, Evalue=1e-29,
Organism=Escherichia coli, GI1790865, Length=151, Percent_Identity=36.4238410596026, Blast_Score=96, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004384
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 30392; Mature: 30261

Theoretical pI: Translated: 6.83; Mature: 6.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGTDKQRPILAEGPAIILVNPQLPENIGMVARAMANFGLAELRLVNPREEFPSEKARAA
CCCCCCCCCEEECCCEEEEECCCCCHHHHHHHHHHHHCCHHHEEECCCHHHCCCHHHHHH
ASKADHVIDNAVVYDDLPSAIADLNFVYATTARERDGFKPVRSAMEAGNALRRRFKTGEK
HHHHHHHHHCHHHHHHHHHHHHHCHHHEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCC
TGILFGRERFGLSNEEVGLADELLTFPVNPAFASLNIAQAVLLMSYEWMKSGLENQTDTV
CEEEEEHHHCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCE
FRGPEMPLAPKEELHGFFNHLEEALDVRGYFRPEARKEIMVNNLRAVLTRAGFASAELKL
EECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHH
LRGVVTSLDVFTPKKPRGSGAPEGRARKPVNEDKAE
HHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
AGTDKQRPILAEGPAIILVNPQLPENIGMVARAMANFGLAELRLVNPREEFPSEKARAA
CCCCCCCCEEECCCEEEEECCCCCHHHHHHHHHHHHCCHHHEEECCCHHHCCCHHHHHH
ASKADHVIDNAVVYDDLPSAIADLNFVYATTARERDGFKPVRSAMEAGNALRRRFKTGEK
HHHHHHHHHCHHHHHHHHHHHHHCHHHEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCC
TGILFGRERFGLSNEEVGLADELLTFPVNPAFASLNIAQAVLLMSYEWMKSGLENQTDTV
CEEEEEHHHCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCE
FRGPEMPLAPKEELHGFFNHLEEALDVRGYFRPEARKEIMVNNLRAVLTRAGFASAELKL
EECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHH
LRGVVTSLDVFTPKKPRGSGAPEGRARKPVNEDKAE
HHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14528314 [H]