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The map label for this gene is nifR3 [H]

Identifier: 23501997

GI number: 23501997

Start: 1097114

End: 1098115

Strand: Reverse

Name: nifR3 [H]

Synonym: BR1119

Alternate gene names: 23501997

Gene position: 1098115-1097114 (Counterclockwise)

Preceding gene: 23502001

Following gene: 23501996

Centisome position: 52.1

GC content: 58.78

Gene sequence:

>1002_bases
TTGGTGACTTCCCTCGATCAACCTTTGAATATTCGCGGTGTGATGCTCAAGAACCGCGTTTTTCTTGCGCCGATGTCCGG
TGTTTCCGATCTCCCGTTTCGCAAGCGCGCGGCTGAGGCCGGTGCGGGTATGGTCGTGTCCGAAATGGTGGCGAGTGCGG
AGCTGTGCAACCGGCACAGGGAAAGTCTGTTGCGCCTTTCCGGCGAGGGGCTGGGAACGCATGTGGTGCAGCTTGCAGGA
CGCGAGGCGCGCTGGATGGGGGAGGCTGCAAAAATCGCCGAAGGCGAGGGTGCGGATATCATTGATATCAATATGGGCTG
CCTTGCGAAAAAGGTGACGGGCGGCTATTCCGGTTCGGCGCTGATGCGCAATCTCGATCATGCCATGACACTGGTTGAGG
CCACGGTTGCGGCGGTGAAAGTACCTGTCACGCTGAAGATGCGACTTGGCTGGGATGAAAACAGCATCAATGCGCCGGAG
CTTGCCAGGCGCGCCGAAGATGCGGGTATCGCCATGGTGACGGTGCATGGCCGCACGCGCTGCCAGTTTTATGAAGGCAC
TGCAGACTGGGATGCGATCCATGCGGTGCGCGATGCGGTGAGAATCCCGCTTGTCGCCAATGGAGATGTCTCATCGCGCG
CCGATGCCGAGGAAATTCTGCGCCGCTCGGGGGCTGACGCTGTAATGGTGGGACGTGCCTCCTATGGGCAGCCATGGCTG
GCGGGAGCGATCGCGGGAAGTGATTTCGCACCGCGTGATGCAAATGGAATTCTTGATTATATTATAAGACATTACGAAGA
TATGCTCGACCATTATGGCAGCAAAATAGGCATTCGTCACGCCCGCGAGCATCTGGGCTGGTATATGGATCGCCATGCCG
GGCGCGCCTTCACGCCGGCAGACAAGGCGGAACTCATGACGTTGACGGATCGGGCTGCAGTGGTTGAAACCCTCCGGCGA
ATATTCCTGCGCGATGTCGATCCGCTGCGAAAGGTTGCCTGA

Upstream 100 bases:

>100_bases
AATTGATTTATCGTGCGTTCAGGCTGCTTTTCCTTGCGTTCGGAAAGCGGGGTGATTAAACTGTATGCGGATTTCATAAT
GCATATCAAATAGGCACTTA

Downstream 100 bases:

>100_bases
TGCGCAATAAGACTGAAACGGGCGGCGTTCCTTCCATCGTGCTTGATGCGATCAGCCAGCCGGTCATCATGGTGAGTGCG
GATAATCACATCGTCTATGC

Product: nitrogen regulation protein Nifr3

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 333; Mature: 333

Protein sequence:

>333_residues
MVTSLDQPLNIRGVMLKNRVFLAPMSGVSDLPFRKRAAEAGAGMVVSEMVASAELCNRHRESLLRLSGEGLGTHVVQLAG
REARWMGEAAKIAEGEGADIIDINMGCLAKKVTGGYSGSALMRNLDHAMTLVEATVAAVKVPVTLKMRLGWDENSINAPE
LARRAEDAGIAMVTVHGRTRCQFYEGTADWDAIHAVRDAVRIPLVANGDVSSRADAEEILRRSGADAVMVGRASYGQPWL
AGAIAGSDFAPRDANGILDYIIRHYEDMLDHYGSKIGIRHAREHLGWYMDRHAGRAFTPADKAELMTLTDRAAVVETLRR
IFLRDVDPLRKVA

Sequences:

>Translated_333_residues
MVTSLDQPLNIRGVMLKNRVFLAPMSGVSDLPFRKRAAEAGAGMVVSEMVASAELCNRHRESLLRLSGEGLGTHVVQLAG
REARWMGEAAKIAEGEGADIIDINMGCLAKKVTGGYSGSALMRNLDHAMTLVEATVAAVKVPVTLKMRLGWDENSINAPE
LARRAEDAGIAMVTVHGRTRCQFYEGTADWDAIHAVRDAVRIPLVANGDVSSRADAEEILRRSGADAVMVGRASYGQPWL
AGAIAGSDFAPRDANGILDYIIRHYEDMLDHYGSKIGIRHAREHLGWYMDRHAGRAFTPADKAELMTLTDRAAVVETLRR
IFLRDVDPLRKVA
>Mature_333_residues
MVTSLDQPLNIRGVMLKNRVFLAPMSGVSDLPFRKRAAEAGAGMVVSEMVASAELCNRHRESLLRLSGEGLGTHVVQLAG
REARWMGEAAKIAEGEGADIIDINMGCLAKKVTGGYSGSALMRNLDHAMTLVEATVAAVKVPVTLKMRLGWDENSINAPE
LARRAEDAGIAMVTVHGRTRCQFYEGTADWDAIHAVRDAVRIPLVANGDVSSRADAEEILRRSGADAVMVGRASYGQPWL
AGAIAGSDFAPRDANGILDYIIRHYEDMLDHYGSKIGIRHAREHLGWYMDRHAGRAFTPADKAELMTLTDRAAVVETLRR
IFLRDVDPLRKVA

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family [H]

Homologues:

Organism=Homo sapiens, GI239788483, Length=288, Percent_Identity=30.9027777777778, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI239788462, Length=284, Percent_Identity=30.2816901408451, Blast_Score=111, Evalue=9e-25,
Organism=Homo sapiens, GI31742496, Length=302, Percent_Identity=26.8211920529801, Blast_Score=98, Evalue=9e-21,
Organism=Homo sapiens, GI8923374, Length=251, Percent_Identity=28.6852589641434, Blast_Score=93, Evalue=3e-19,
Organism=Homo sapiens, GI40807366, Length=233, Percent_Identity=30.0429184549356, Blast_Score=89, Evalue=7e-18,
Organism=Escherichia coli, GI1789660, Length=291, Percent_Identity=41.2371134020619, Blast_Score=222, Evalue=2e-59,
Organism=Escherichia coli, GI1788462, Length=234, Percent_Identity=34.1880341880342, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI145693211, Length=200, Percent_Identity=27, Blast_Score=70, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17543114, Length=288, Percent_Identity=29.8611111111111, Blast_Score=110, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI25144369, Length=235, Percent_Identity=29.3617021276596, Blast_Score=88, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI17507177, Length=226, Percent_Identity=30.9734513274336, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17510279, Length=247, Percent_Identity=25.9109311740891, Blast_Score=76, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6323433, Length=304, Percent_Identity=26.9736842105263, Blast_Score=93, Evalue=5e-20,
Organism=Saccharomyces cerevisiae, GI6323560, Length=256, Percent_Identity=30.859375, Blast_Score=91, Evalue=4e-19,
Organism=Saccharomyces cerevisiae, GI6323437, Length=260, Percent_Identity=27.6923076923077, Blast_Score=82, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24585320, Length=281, Percent_Identity=30.2491103202847, Blast_Score=103, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24580595, Length=239, Percent_Identity=30.1255230125523, Blast_Score=101, Evalue=8e-22,
Organism=Drosophila melanogaster, GI19920448, Length=239, Percent_Identity=30.1255230125523, Blast_Score=101, Evalue=8e-22,
Organism=Drosophila melanogaster, GI45549423, Length=241, Percent_Identity=29.8755186721992, Blast_Score=89, Evalue=5e-18,
Organism=Drosophila melanogaster, GI19921524, Length=318, Percent_Identity=25.7861635220126, Blast_Score=85, Evalue=7e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004652
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 36285; Mature: 36285

Theoretical pI: Translated: 7.35; Mature: 7.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVTSLDQPLNIRGVMLKNRVFLAPMSGVSDLPFRKRAAEAGAGMVVSEMVASAELCNRHR
CCCCCCCCCCEEEEEEECEEEEEECCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHH
ESLLRLSGEGLGTHVVQLAGREARWMGEAAKIAEGEGADIIDINMGCLAKKVTGGYSGSA
HHHHHHCCCCCHHHHHHHCCCHHHHHHHHHHHCCCCCCCEEEECHHHHHHHHCCCCCHHH
LMRNLDHAMTLVEATVAAVKVPVTLKMRLGWDENSINAPELARRAEDAGIAMVTVHGRTR
HHHHHHHHHHHHHHHHHHEECCEEEEEEECCCCCCCCHHHHHHHHCCCCEEEEEECCCEE
CQFYEGTADWDAIHAVRDAVRIPLVANGDVSSRADAEEILRRSGADAVMVGRASYGQPWL
EEEECCCCCHHHHHHHHHHHHCCEEECCCCCCCCCHHHHHHHCCCCEEEECCCCCCCCHH
AGAIAGSDFAPRDANGILDYIIRHYEDMLDHYGSKIGIRHAREHLGWYMDRHAGRAFTPA
HHEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCC
DKAELMTLTDRAAVVETLRRIFLRDVDPLRKVA
CCHHEEEHHHHHHHHHHHHHHHHHCCCHHHHCC
>Mature Secondary Structure
MVTSLDQPLNIRGVMLKNRVFLAPMSGVSDLPFRKRAAEAGAGMVVSEMVASAELCNRHR
CCCCCCCCCCEEEEEEECEEEEEECCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHH
ESLLRLSGEGLGTHVVQLAGREARWMGEAAKIAEGEGADIIDINMGCLAKKVTGGYSGSA
HHHHHHCCCCCHHHHHHHCCCHHHHHHHHHHHCCCCCCCEEEECHHHHHHHHCCCCCHHH
LMRNLDHAMTLVEATVAAVKVPVTLKMRLGWDENSINAPELARRAEDAGIAMVTVHGRTR
HHHHHHHHHHHHHHHHHHEECCEEEEEEECCCCCCCCHHHHHHHHCCCCEEEEEECCCEE
CQFYEGTADWDAIHAVRDAVRIPLVANGDVSSRADAEEILRRSGADAVMVGRASYGQPWL
EEEECCCCCHHHHHHHHHHHHCCEEECCCCCCCCCHHHHHHHCCCCEEEECCCCCCCCHH
AGAIAGSDFAPRDANGILDYIIRHYEDMLDHYGSKIGIRHAREHLGWYMDRHAGRAFTPA
HHEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCC
DKAELMTLTDRAAVVETLRRIFLRDVDPLRKVA
CCHHEEEHHHHHHHHHHHHHHHHHCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8412703 [H]