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The map label for this gene is gdhA [H]

Identifier: 23501134

GI number: 23501134

Start: 238479

End: 239744

Strand: Reverse

Name: gdhA [H]

Synonym: BR0227

Alternate gene names: 23501134

Gene position: 239744-238479 (Counterclockwise)

Preceding gene: 23501140

Following gene: 23501133

Centisome position: 11.37

GC content: 60.66

Gene sequence:

>1266_bases
GTGACCACGGAAAATCTGCTCGAAAGTGCACTCGTCCGGCTGGACGAAGCGGCAAGCCACATAAACATCGATGCGGATGT
CATCGAAAAGCTGAAGTTTGCGCGTGAGACCATGAAAGTGCGGTTGATGATCCGCATGGACGACGGTTCGCGCAAGTCCT
TTATTGCGTGGCGCTGCCGCTATGACGACACTCGCGGACCGACCAAGGGCGGCATCCGCTACCATCCCGATTCAACGGTG
GAAGAAGTCGAGACGCTCGCCTTCTGGATGACCTTCAAATGCGCCGTGATGAACCTGCCTTATGGCGGCGGCAAGGGCGC
GATCCAGGTCGATCCGCGCCAGCTCTCCAAGGCTGAACTGGAGCGCCTTTCGCGCGCCTATATCCAGGCTTTCTCCGGCA
TCATCGGCCCGGACCGCGACATTCCGGCCCCGGACGTCTATACGAATTCCATGATCATGGGCTGGATGGCTGACGAATAC
TCCCAGATCGTCGGCCAGTCCTCTCCCGCCGTCATTACCGGCAAGCCCATCGCACTTGGTGGCTCGCTCGGACGCAATGA
CGCGACCGCACGCGGCGGCTTCTACCTCGTTCGCCACCTCTCGCACGATCTGGGCCTCGCTTCGGTTCTGCGCGTCGCCA
TCCAGGGTTTTGGCAATGCCGGACAGTTCATGGCCAAGCTGATGGCGGGCGACGGCCACAAGATCGTCGCCGTGTCGGAT
TCCGCAGGGGCGGTCTATTGCGCGGATGGCCTTGACGTCGACCTGCTTCTGGCCGCCAAGGCTGATGGCAAGTCCGTCAT
TTCAACCGCCGGTCACAAGGGCCACGAGGCGATCAGCGCCGACGAACTCGTCGCCGCCGATTGTGACGTGCTGGTGCCAA
GCGCCATGGAAAACATGATCCATGCCAGTAATGCAGCTTCCATTCGCGCCAAGCTTATCGTGGAACTGGCCAACGGCCCG
GTCACGGGGGACGCTGACAAGATCCTCGCCGAAAAGGGTGTGATGGTCCTGCCGGACATTCTTGCCAATGCTGGCGGCGT
GACGGTTTCCTATTTTGAATGGGTGCAGAACCGTCAGGGTTATTACTGGACGCTTGAAGAAATCCATGAACGCCTGAAGA
CGATCATGGAACGCGAAGGCCGCGCCATCTGGAACCATGCCCGCGAACGCGGTGTGACCCTGCGCACGGCTGCCTATGTG
CATGCGCTGGAGCGCCTTGCACAAGCGATCGAGGCCCACGGCACGCAAAACGACTTCGCGGCCTGA

Upstream 100 bases:

>100_bases
ACACTATTGTCCTTTTTGCTTTCCTTTTGTTGCGCAAAGGCGTAGTCAGAAATACGCAAAATCGCATTTTTAAAAACATA
CACGCAAGAAAGGTGATTTC

Downstream 100 bases:

>100_bases
TTCACTCTACGAACGTTACCCCTCCTGTCGGATTACCGGCGGGAGGGGACAAAAAGCGGTTTCTTTTTCCGCCCGCACGA
CAATCTTGAGCTGCTGGCGC

Product: glutamate dehydrogenase

Products: NA

Alternate protein names: NADP-GDH [H]

Number of amino acids: Translated: 421; Mature: 420

Protein sequence:

>421_residues
MTTENLLESALVRLDEAASHINIDADVIEKLKFARETMKVRLMIRMDDGSRKSFIAWRCRYDDTRGPTKGGIRYHPDSTV
EEVETLAFWMTFKCAVMNLPYGGGKGAIQVDPRQLSKAELERLSRAYIQAFSGIIGPDRDIPAPDVYTNSMIMGWMADEY
SQIVGQSSPAVITGKPIALGGSLGRNDATARGGFYLVRHLSHDLGLASVLRVAIQGFGNAGQFMAKLMAGDGHKIVAVSD
SAGAVYCADGLDVDLLLAAKADGKSVISTAGHKGHEAISADELVAADCDVLVPSAMENMIHASNAASIRAKLIVELANGP
VTGDADKILAEKGVMVLPDILANAGGVTVSYFEWVQNRQGYYWTLEEIHERLKTIMEREGRAIWNHARERGVTLRTAAYV
HALERLAQAIEAHGTQNDFAA

Sequences:

>Translated_421_residues
MTTENLLESALVRLDEAASHINIDADVIEKLKFARETMKVRLMIRMDDGSRKSFIAWRCRYDDTRGPTKGGIRYHPDSTV
EEVETLAFWMTFKCAVMNLPYGGGKGAIQVDPRQLSKAELERLSRAYIQAFSGIIGPDRDIPAPDVYTNSMIMGWMADEY
SQIVGQSSPAVITGKPIALGGSLGRNDATARGGFYLVRHLSHDLGLASVLRVAIQGFGNAGQFMAKLMAGDGHKIVAVSD
SAGAVYCADGLDVDLLLAAKADGKSVISTAGHKGHEAISADELVAADCDVLVPSAMENMIHASNAASIRAKLIVELANGP
VTGDADKILAEKGVMVLPDILANAGGVTVSYFEWVQNRQGYYWTLEEIHERLKTIMEREGRAIWNHARERGVTLRTAAYV
HALERLAQAIEAHGTQNDFAA
>Mature_420_residues
TTENLLESALVRLDEAASHINIDADVIEKLKFARETMKVRLMIRMDDGSRKSFIAWRCRYDDTRGPTKGGIRYHPDSTVE
EVETLAFWMTFKCAVMNLPYGGGKGAIQVDPRQLSKAELERLSRAYIQAFSGIIGPDRDIPAPDVYTNSMIMGWMADEYS
QIVGQSSPAVITGKPIALGGSLGRNDATARGGFYLVRHLSHDLGLASVLRVAIQGFGNAGQFMAKLMAGDGHKIVAVSDS
AGAVYCADGLDVDLLLAAKADGKSVISTAGHKGHEAISADELVAADCDVLVPSAMENMIHASNAASIRAKLIVELANGPV
TGDADKILAEKGVMVLPDILANAGGVTVSYFEWVQNRQGYYWTLEEIHERLKTIMEREGRAIWNHARERGVTLRTAAYVH
ALERLAQAIEAHGTQNDFAA

Specific function: Unknown

COG id: COG0334

COG function: function code E; Glutamate dehydrogenase/leucine dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Glu/Leu/Phe/Val dehydrogenases family [H]

Homologues:

Organism=Homo sapiens, GI4885281, Length=454, Percent_Identity=34.5814977973568, Blast_Score=261, Evalue=1e-69,
Organism=Homo sapiens, GI31377775, Length=454, Percent_Identity=34.1409691629956, Blast_Score=260, Evalue=2e-69,
Organism=Escherichia coli, GI1788059, Length=360, Percent_Identity=30.5555555555556, Blast_Score=161, Evalue=6e-41,
Organism=Caenorhabditis elegans, GI17544676, Length=476, Percent_Identity=32.9831932773109, Blast_Score=251, Evalue=4e-67,
Organism=Saccharomyces cerevisiae, GI6319256, Length=413, Percent_Identity=28.5714285714286, Blast_Score=130, Evalue=6e-31,
Organism=Saccharomyces cerevisiae, GI6324951, Length=366, Percent_Identity=28.4153005464481, Blast_Score=125, Evalue=1e-29,
Organism=Drosophila melanogaster, GI45553475, Length=435, Percent_Identity=32.4137931034483, Blast_Score=245, Evalue=4e-65,
Organism=Drosophila melanogaster, GI45549226, Length=448, Percent_Identity=31.0267857142857, Blast_Score=238, Evalue=7e-63,
Organism=Drosophila melanogaster, GI24649283, Length=373, Percent_Identity=35.9249329758713, Blast_Score=230, Evalue=2e-60,
Organism=Drosophila melanogaster, GI24649460, Length=396, Percent_Identity=31.3131313131313, Blast_Score=207, Evalue=9e-54,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 9362 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006095
- InterPro:   IPR006096
- InterPro:   IPR006097
- InterPro:   IPR014362
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00208 ELFV_dehydrog; PF02812 ELFV_dehydrog_N [H]

EC number: =1.4.1.4 [H]

Molecular weight: Translated: 45660; Mature: 45528

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTENLLESALVRLDEAASHINIDADVIEKLKFARETMKVRLMIRMDDGSRKSFIAWRCR
CCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCEEEEEEEEECCCCCCCEEEEEEE
YDDTRGPTKGGIRYHPDSTVEEVETLAFWMTFKCAVMNLPYGGGKGAIQVDPRQLSKAEL
CCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHEEECCCCCCCCCEEECHHHHHHHHH
ERLSRAYIQAFSGIIGPDRDIPAPDVYTNSMIMGWMADEYSQIVGQSSPAVITGKPIALG
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHCCCCCEEEECCCEEEC
GSLGRNDATARGGFYLVRHLSHDLGLASVLRVAIQGFGNAGQFMAKLMAGDGHKIVAVSD
CCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHCCCCCEEEEEEC
SAGAVYCADGLDVDLLLAAKADGKSVISTAGHKGHEAISADELVAADCDVLVPSAMENMI
CCCEEEEECCCCEEEEEEECCCCHHHHHHCCCCCCCCCCCCHHHHCCCCEECHHHHHHHH
HASNAASIRAKLIVELANGPVTGDADKILAEKGVMVLPDILANAGGVTVSYFEWVQNRQG
HCCCCHHHEEEEEEEECCCCCCCCHHHHHHHCCCEEEHHHHCCCCCCCHHHHHHHHCCCC
YYWTLEEIHERLKTIMEREGRAIWNHARERGVTLRTAAYVHALERLAQAIEAHGTQNDFA
EEEEHHHHHHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCC
A
C
>Mature Secondary Structure 
TTENLLESALVRLDEAASHINIDADVIEKLKFARETMKVRLMIRMDDGSRKSFIAWRCR
CHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCEEEEEEEEECCCCCCCEEEEEEE
YDDTRGPTKGGIRYHPDSTVEEVETLAFWMTFKCAVMNLPYGGGKGAIQVDPRQLSKAEL
CCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHEEECCCCCCCCCEEECHHHHHHHHH
ERLSRAYIQAFSGIIGPDRDIPAPDVYTNSMIMGWMADEYSQIVGQSSPAVITGKPIALG
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHCCCCCEEEECCCEEEC
GSLGRNDATARGGFYLVRHLSHDLGLASVLRVAIQGFGNAGQFMAKLMAGDGHKIVAVSD
CCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHCCCCCEEEEEEC
SAGAVYCADGLDVDLLLAAKADGKSVISTAGHKGHEAISADELVAADCDVLVPSAMENMI
CCCEEEEECCCCEEEEEEECCCCHHHHHHCCCCCCCCCCCCHHHHCCCCEECHHHHHHHH
HASNAASIRAKLIVELANGPVTGDADKILAEKGVMVLPDILANAGGVTVSYFEWVQNRQG
HCCCCHHHEEEEEEEECCCCCCCCHHHHHHHCCCEEEHHHHCCCCCCCHHHHHHHHCCCC
YYWTLEEIHERLKTIMEREGRAIWNHARERGVTLRTAAYVHALERLAQAIEAHGTQNDFA
EEEEHHHHHHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCC
A
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7787182; 8590279; 8905231 [H]