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The map label for this gene is acsA1 [H]

Identifier: 23500936

GI number: 23500936

Start: 24189

End: 26177

Strand: Reverse

Name: acsA1 [H]

Synonym: BR0021

Alternate gene names: 23500936

Gene position: 26177-24189 (Counterclockwise)

Preceding gene: 23500937

Following gene: 23500935

Centisome position: 1.24

GC content: 59.98

Gene sequence:

>1989_bases
ATGAGCACCGAGGCGATCTCCAGACAGTCTTCGCATGACAATCCGCTGTGGCAACCGGATGGCGCGCGCATTGCGGCCAG
CAATCTCGAACAATTCCGCATTCGCGCCGAACGGCATACCGGCCTCAGCCTGCCGGATTATGCCGCGCTGCATCGCTGGT
CTGTAGAGGATCGCGCCGGGTTCTGGCAACTTGTATGGGATTTCTGCTCGGTTATCGGAGAGCGTGGCAATACGGCCCTT
ACCGACAGGGGCCATATGCGCGCGGCAAAATTCTTCCCCGAGGCGCGGCTCAACTTTGCCGAAAACCTGTTGCGCCACAA
GGGCGATGGGGAGGCCATTGTCTTTCGCGGGGAGAATAAGGTCGAGCGCCGCCTGACCTGGAACGATCTTCATGCGCTGG
TCTCGAAGCTACAGCAATTCATGCTTTCCGAGGGCGTGCAGCCGGGCGACCGTGTGGCGGGCATGATGCCCAACATGCCG
GAGGCGGTGGCGCTGATGCTGGCGGCCTCGTCCATCGGTGCGGTCTGGTCGTCATGCTCGCCGGATTTTGGCGTGCAGGG
CGTGCTTGACCGTTTCGGCCAGATCGAGCCGAAACTGTTCTTCGCCTGCGATGGCTACTGGTATAATGGCAAGCGCATCG
CGGTTGCCGACAAGGTGGCTGAAGTGACGGCAAAACTGCCGGGACTGAAGCGCGCGGTCATCGTCACCTATCTTGGCGAA
GCGGAAGCCGTCGCCGACAAGGCGGAAAAGGGCATTGCACTTTATGCGGCGCTGGAGCCATTCAAGGCCAAGCCCGTGGA
ATTCACGCAGCTTCCCTTCGATCATCCGCTTTATATCCTGTTTTCGTCGGGCACGACCGGCATTCCGAAATGCATCGTGC
ATCGGGCTGGCGGCGTGCTGCTGCAACACCTGAAGGAACATCGCCTTCATGCCGACCTGCGCGATGGCGACCGCTTCTTC
TATTTCACCACCTGCGGCTGGATGATGTGGAACTGGCTGGCTTCTGGCATCGCCTCCGGCGCCACGCTTATGCTCTATGA
CGGCTCGCCCTTCTATCCGGACGGCAATGTGCTGTTCGATTATGCCGCTGCTGAGGGCATGACCTATTTCGGCACGTCGG
CAAAATTCATCGATGCCGTGCTGAAAGCGGGATTGAAGCCCGGAGAAACGCACGACCTTTCGGCTCTGCGCACCATTTCC
TCCACCGGCTCGCCGCTGTCGCCGGAAGGCTTTGCCTTCGTCTATGATGCGATCAAGAAGGATGTGCATCTCGCCTCCAT
TTCGGGCGGAACGGACATTGTGTCCTGCTTCGTGCTGGGCGTTCCGACCGAGCCTGTCTGGCAGGGCGAGATACAGGGTG
CCGGGCTCGGCATGGCTGTCGATGTCTGGAACGATGACGGCAAGCCGGTGCGGGGCGAAAAGGGTGAACTGGTCTGCACG
AAGGCATTTCCTTCCATGCCGCTGCAATTCTGGAACGACGCGCAAGGCGAAAAATATCAGGCCGCCTATTTCGAGCGTTT
CGACAATATCTGGTGCCATGGCGATTTTGCCGAATGGACCGAACATGGCGGCATCGTCATCCATGGCCGCTCGGACGCGA
CGCTGAACCCCGGCGGCGTGCGCATCGGCACGGCGGAGATCTACAATCAGGTCGAGCAGATGCCGGAAATTGCCGAGGCG
CTCTGCATCGGACAGGATTGGGACAGGGATGTGCGGGTGGTGCTTTTCGTACGGCTTGCCGAAGGGGTGGTGCTGGATGA
CGACCTCAAGGCGCGCATCAGGACCAAAATCCGCACCGGCGCCACACCGCGCCATGTACCGGCGAAAATCATTGCCGTCC
GCGATATTCCGCGCACCAAATCGGGAAAGATCGTGGAACTTGCCGTCCGCGATATCGTGCATGGTCGCGAGGTGAAGAAC
CGTGAAGCATTGGCCAACCCGGAAGCGCTGGAATTTTATCGCGACATAGCCGAGTTGCAGCAGGATTGA

Upstream 100 bases:

>100_bases
TTTTCGCAAAGCCTATGGACGTGCCTGAAAGTCTCTCATAGAAAATATTGGAATGAGTTGATGAACCCTCATCGGCTCAG
CTTGATTTCTGGGAGGAATG

Downstream 100 bases:

>100_bases
TGCGAAATAAAATTTCTTTATTGGGCAATAAATAGCCACAGAACGCCACGCAAAACCATTGCGTATTTGGCCGCTACGGG
ACACTCTGGCCGGGACATAG

Product: acetoacetyl-CoA synthetase

Products: NA

Alternate protein names: Acetoacetate--CoA ligase 1; Acyl-activating enzyme 1 [H]

Number of amino acids: Translated: 662; Mature: 661

Protein sequence:

>662_residues
MSTEAISRQSSHDNPLWQPDGARIAASNLEQFRIRAERHTGLSLPDYAALHRWSVEDRAGFWQLVWDFCSVIGERGNTAL
TDRGHMRAAKFFPEARLNFAENLLRHKGDGEAIVFRGENKVERRLTWNDLHALVSKLQQFMLSEGVQPGDRVAGMMPNMP
EAVALMLAASSIGAVWSSCSPDFGVQGVLDRFGQIEPKLFFACDGYWYNGKRIAVADKVAEVTAKLPGLKRAVIVTYLGE
AEAVADKAEKGIALYAALEPFKAKPVEFTQLPFDHPLYILFSSGTTGIPKCIVHRAGGVLLQHLKEHRLHADLRDGDRFF
YFTTCGWMMWNWLASGIASGATLMLYDGSPFYPDGNVLFDYAAAEGMTYFGTSAKFIDAVLKAGLKPGETHDLSALRTIS
STGSPLSPEGFAFVYDAIKKDVHLASISGGTDIVSCFVLGVPTEPVWQGEIQGAGLGMAVDVWNDDGKPVRGEKGELVCT
KAFPSMPLQFWNDAQGEKYQAAYFERFDNIWCHGDFAEWTEHGGIVIHGRSDATLNPGGVRIGTAEIYNQVEQMPEIAEA
LCIGQDWDRDVRVVLFVRLAEGVVLDDDLKARIRTKIRTGATPRHVPAKIIAVRDIPRTKSGKIVELAVRDIVHGREVKN
REALANPEALEFYRDIAELQQD

Sequences:

>Translated_662_residues
MSTEAISRQSSHDNPLWQPDGARIAASNLEQFRIRAERHTGLSLPDYAALHRWSVEDRAGFWQLVWDFCSVIGERGNTAL
TDRGHMRAAKFFPEARLNFAENLLRHKGDGEAIVFRGENKVERRLTWNDLHALVSKLQQFMLSEGVQPGDRVAGMMPNMP
EAVALMLAASSIGAVWSSCSPDFGVQGVLDRFGQIEPKLFFACDGYWYNGKRIAVADKVAEVTAKLPGLKRAVIVTYLGE
AEAVADKAEKGIALYAALEPFKAKPVEFTQLPFDHPLYILFSSGTTGIPKCIVHRAGGVLLQHLKEHRLHADLRDGDRFF
YFTTCGWMMWNWLASGIASGATLMLYDGSPFYPDGNVLFDYAAAEGMTYFGTSAKFIDAVLKAGLKPGETHDLSALRTIS
STGSPLSPEGFAFVYDAIKKDVHLASISGGTDIVSCFVLGVPTEPVWQGEIQGAGLGMAVDVWNDDGKPVRGEKGELVCT
KAFPSMPLQFWNDAQGEKYQAAYFERFDNIWCHGDFAEWTEHGGIVIHGRSDATLNPGGVRIGTAEIYNQVEQMPEIAEA
LCIGQDWDRDVRVVLFVRLAEGVVLDDDLKARIRTKIRTGATPRHVPAKIIAVRDIPRTKSGKIVELAVRDIVHGREVKN
REALANPEALEFYRDIAELQQD
>Mature_661_residues
STEAISRQSSHDNPLWQPDGARIAASNLEQFRIRAERHTGLSLPDYAALHRWSVEDRAGFWQLVWDFCSVIGERGNTALT
DRGHMRAAKFFPEARLNFAENLLRHKGDGEAIVFRGENKVERRLTWNDLHALVSKLQQFMLSEGVQPGDRVAGMMPNMPE
AVALMLAASSIGAVWSSCSPDFGVQGVLDRFGQIEPKLFFACDGYWYNGKRIAVADKVAEVTAKLPGLKRAVIVTYLGEA
EAVADKAEKGIALYAALEPFKAKPVEFTQLPFDHPLYILFSSGTTGIPKCIVHRAGGVLLQHLKEHRLHADLRDGDRFFY
FTTCGWMMWNWLASGIASGATLMLYDGSPFYPDGNVLFDYAAAEGMTYFGTSAKFIDAVLKAGLKPGETHDLSALRTISS
TGSPLSPEGFAFVYDAIKKDVHLASISGGTDIVSCFVLGVPTEPVWQGEIQGAGLGMAVDVWNDDGKPVRGEKGELVCTK
AFPSMPLQFWNDAQGEKYQAAYFERFDNIWCHGDFAEWTEHGGIVIHGRSDATLNPGGVRIGTAEIYNQVEQMPEIAEAL
CIGQDWDRDVRVVLFVRLAEGVVLDDDLKARIRTKIRTGATPRHVPAKIIAVRDIPRTKSGKIVELAVRDIVHGREVKNR
EALANPEALEFYRDIAELQQD

Specific function: Involved in poly-3-hydroxybutyrate degradation. Activates acetoacetate to acetoacetyl-CoA [H]

COG id: COG0365

COG function: function code I; Acyl-coenzyme A synthetases/AMP-(fatty) acid ligases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI31982927, Length=655, Percent_Identity=46.8702290076336, Blast_Score=594, Evalue=1e-170,
Organism=Homo sapiens, GI28416953, Length=649, Percent_Identity=26.502311248074, Blast_Score=175, Evalue=2e-43,
Organism=Homo sapiens, GI8923896, Length=659, Percent_Identity=25.7966616084977, Blast_Score=173, Evalue=6e-43,
Organism=Homo sapiens, GI253970489, Length=672, Percent_Identity=25, Blast_Score=170, Evalue=3e-42,
Organism=Homo sapiens, GI13375727, Length=618, Percent_Identity=24.4336569579288, Blast_Score=143, Evalue=4e-34,
Organism=Homo sapiens, GI38505220, Length=498, Percent_Identity=22.0883534136546, Blast_Score=92, Evalue=2e-18,
Organism=Homo sapiens, GI122937307, Length=563, Percent_Identity=21.3143872113677, Blast_Score=79, Evalue=1e-14,
Organism=Escherichia coli, GI1790505, Length=630, Percent_Identity=27.1428571428571, Blast_Score=192, Evalue=7e-50,
Organism=Escherichia coli, GI1786529, Length=600, Percent_Identity=24.3333333333333, Blast_Score=127, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI17569507, Length=644, Percent_Identity=38.5093167701863, Blast_Score=461, Evalue=1e-130,
Organism=Caenorhabditis elegans, GI71983001, Length=641, Percent_Identity=25.8970358814353, Blast_Score=163, Evalue=3e-40,
Organism=Caenorhabditis elegans, GI71982997, Length=641, Percent_Identity=25.8970358814353, Blast_Score=162, Evalue=4e-40,
Organism=Saccharomyces cerevisiae, GI6319264, Length=656, Percent_Identity=26.9817073170732, Blast_Score=180, Evalue=6e-46,
Organism=Saccharomyces cerevisiae, GI6323182, Length=592, Percent_Identity=26.1824324324324, Blast_Score=172, Evalue=1e-43,
Organism=Drosophila melanogaster, GI24667955, Length=644, Percent_Identity=25.9316770186335, Blast_Score=194, Evalue=2e-49,
Organism=Drosophila melanogaster, GI62472339, Length=552, Percent_Identity=26.9927536231884, Blast_Score=188, Evalue=1e-47,
Organism=Drosophila melanogaster, GI24667959, Length=519, Percent_Identity=26.7822736030828, Blast_Score=171, Evalue=2e-42,
Organism=Drosophila melanogaster, GI21355225, Length=629, Percent_Identity=23.6883942766296, Blast_Score=120, Evalue=4e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005914
- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.16 [H]

Molecular weight: Translated: 73218; Mature: 73087

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTEAISRQSSHDNPLWQPDGARIAASNLEQFRIRAERHTGLSLPDYAALHRWSVEDRAG
CCCHHHCCCCCCCCCCCCCCCCEEECCCHHHHHHHHHHCCCCCCCCHHHHHCCCCHHHCH
FWQLVWDFCSVIGERGNTALTDRGHMRAAKFFPEARLNFAENLLRHKGDGEAIVFRGENK
HHHHHHHHHHHHCCCCCEEECCCCCHHHHHHCCHHHHHHHHHHHHHCCCCCEEEEECCCH
VERRLTWNDLHALVSKLQQFMLSEGVQPGDRVAGMMPNMPEAVALMLAASSIGAVWSSCS
HHHHCCHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCC
PDFGVQGVLDRFGQIEPKLFFACDGYWYNGKRIAVADKVAEVTAKLPGLKRAVIVTYLGE
CCCCHHHHHHHHCCCCCEEEEEECCEEECCEEEEEHHHHHHHHHHCCCCCEEEEEEECCC
AEAVADKAEKGIALYAALEPFKAKPVEFTQLPFDHPLYILFSSGTTGIPKCIVHRAGGVL
HHHHHHHHHCCEEEEEECCCCCCCCCEEEECCCCCCEEEEECCCCCCCHHHHHHHHHHHH
LQHLKEHRLHADLRDGDRFFYFTTCGWMMWNWLASGIASGATLMLYDGSPFYPDGNVLFD
HHHHHHHHHCCCCCCCCEEEEEEHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEEE
YAAAEGMTYFGTSAKFIDAVLKAGLKPGETHDLSALRTISSTGSPLSPEGFAFVYDAIKK
EHHHCCCEEECCCHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHC
DVHLASISGGTDIVSCFVLGVPTEPVWQGEIQGAGLGMAVDVWNDDGKPVRGEKGELVCT
CEEEEEECCCHHEEEEEEECCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCEEEE
KAFPSMPLQFWNDAQGEKYQAAYFERFDNIWCHGDFAEWTEHGGIVIHGRSDATLNPGGV
CCCCCCCHHHCCCCCCCCHHHHHHHHHCCEEECCCHHHHHHCCCEEEECCCCCEECCCCE
RIGTAEIYNQVEQMPEIAEALCIGQDWDRDVRVVLFVRLAEGVVLDDDLKARIRTKIRTG
EEEHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEHHCCEEECCCHHHHHHHHHHCC
ATPRHVPAKIIAVRDIPRTKSGKIVELAVRDIVHGREVKNREALANPEALEFYRDIAELQ
CCCCCCCEEEEEEECCCCCCCCCEEEHHHHHHHCCCCCCCHHHHCCHHHHHHHHHHHHHH
QD
CC
>Mature Secondary Structure 
STEAISRQSSHDNPLWQPDGARIAASNLEQFRIRAERHTGLSLPDYAALHRWSVEDRAG
CCHHHCCCCCCCCCCCCCCCCEEECCCHHHHHHHHHHCCCCCCCCHHHHHCCCCHHHCH
FWQLVWDFCSVIGERGNTALTDRGHMRAAKFFPEARLNFAENLLRHKGDGEAIVFRGENK
HHHHHHHHHHHHCCCCCEEECCCCCHHHHHHCCHHHHHHHHHHHHHCCCCCEEEEECCCH
VERRLTWNDLHALVSKLQQFMLSEGVQPGDRVAGMMPNMPEAVALMLAASSIGAVWSSCS
HHHHCCHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCC
PDFGVQGVLDRFGQIEPKLFFACDGYWYNGKRIAVADKVAEVTAKLPGLKRAVIVTYLGE
CCCCHHHHHHHHCCCCCEEEEEECCEEECCEEEEEHHHHHHHHHHCCCCCEEEEEEECCC
AEAVADKAEKGIALYAALEPFKAKPVEFTQLPFDHPLYILFSSGTTGIPKCIVHRAGGVL
HHHHHHHHHCCEEEEEECCCCCCCCCEEEECCCCCCEEEEECCCCCCCHHHHHHHHHHHH
LQHLKEHRLHADLRDGDRFFYFTTCGWMMWNWLASGIASGATLMLYDGSPFYPDGNVLFD
HHHHHHHHHCCCCCCCCEEEEEEHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEEE
YAAAEGMTYFGTSAKFIDAVLKAGLKPGETHDLSALRTISSTGSPLSPEGFAFVYDAIKK
EHHHCCCEEECCCHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHC
DVHLASISGGTDIVSCFVLGVPTEPVWQGEIQGAGLGMAVDVWNDDGKPVRGEKGELVCT
CEEEEEECCCHHEEEEEEECCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCEEEE
KAFPSMPLQFWNDAQGEKYQAAYFERFDNIWCHGDFAEWTEHGGIVIHGRSDATLNPGGV
CCCCCCCHHHCCCCCCCCHHHHHHHHHCCEEECCCHHHHHHCCCEEEECCCCCEECCCCE
RIGTAEIYNQVEQMPEIAEALCIGQDWDRDVRVVLFVRLAEGVVLDDDLKARIRTKIRTG
EEEHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEHHCCEEECCCHHHHHHHHHHCC
ATPRHVPAKIIAVRDIPRTKSGKIVELAVRDIVHGREVKNREALANPEALEFYRDIAELQ
CCCCCCCEEEEEEECCCCCCCCCEEEHHHHHHHCCCCCCCHHHHCCHHHHHHHHHHHHHH
QD
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10735852; 11481430 [H]