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The map label for this gene is paaF [C]

Identifier: 23500932

GI number: 23500932

Start: 17578

End: 18372

Strand: Reverse

Name: paaF [C]

Synonym: BR0016

Alternate gene names: 23500932

Gene position: 18372-17578 (Counterclockwise)

Preceding gene: 23500933

Following gene: 23500930

Centisome position: 0.87

GC content: 61.64

Gene sequence:

>795_bases
ATGAATGACTTTGAGACGCTCCATATTGCCGTCGATCAGCGCGGCGTGGCCAGGCTGACGCTCAACCGGCCCGAACAGCA
CAATGCGCTTTCTGGTCGGATGATTGATGAACTGACGACGGCAGCACTGCATCTGGCGGACAATGAAGCCGTGCGCATCG
TGATCCTGACCGGCGCAGGCACAAGCTTTTGTGCGGGCGGCGACCTTGGCTGGATGCGCGAACAGGTGAATGCCACCCGC
GCGCAACGCATTGAGGAGGCGCGCAAACTCGCCCTCATGCTGAAAGCCCTGCGCGACCTGCCAAAACCACTGATCGGGCG
CATCAACGGGCAGGCCTATGGCGGCGGTGTGGGCCTTATCAGTGTATGCGATGCGGCGATCAGCATTTCCGGCGCGCGCT
TTGGCCTGACGGAGACAAAGCTTGGTCTGATACCCGCTACCATCAGCCCCTATGTCGTGGCGCGCATCGGTGAGGCCAAT
GCGTTGCGCACCTTCACATCCGCCCGCCTTTTCGATGCCGAGGAAGGGCGGCGCATCGGCCTCCTGCATGATGTGGTCGA
GGCGGAAAGGCTCGATGCGGCGGTGGAAGCAGAGATAAAACCATACTTTTCCACCGCGCCCGCAGCGGTTGCCGCCTCCA
AGCGGCTGGTTCATGCGCTGGGCGCACCGATAGACGAGGCGGTGATCGACATGACGCTCACTCGCCTTGCCGATACATGG
GAAACACCGGAGGCCGCGGAAGGAATCGCGGCCTTTTTTGCGAAACGAGCACCAGCATGGAAGGGAGGGGACTAA

Upstream 100 bases:

>100_bases
TGGAAATGCTGCATGAAATGGGTTTTGAAACCGGCCTCGATCTGGACAGGCTTCGGTCGGCGGGTTTGTTTGCACAAGCT
TTGCGTCAGGATAAAGCGGC

Downstream 100 bases:

>100_bases
GAGTCTGAATCAAAAAGCGGCAGGCCGAGGCGAAAATGGTGATTTTCGAGTACCGGAGCGGAGTGTACTGAACGTACATG
AGCACCGGAACGCTGAAAAG

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 264; Mature: 264

Protein sequence:

>264_residues
MNDFETLHIAVDQRGVARLTLNRPEQHNALSGRMIDELTTAALHLADNEAVRIVILTGAGTSFCAGGDLGWMREQVNATR
AQRIEEARKLALMLKALRDLPKPLIGRINGQAYGGGVGLISVCDAAISISGARFGLTETKLGLIPATISPYVVARIGEAN
ALRTFTSARLFDAEEGRRIGLLHDVVEAERLDAAVEAEIKPYFSTAPAAVAASKRLVHALGAPIDEAVIDMTLTRLADTW
ETPEAAEGIAAFFAKRAPAWKGGD

Sequences:

>Translated_264_residues
MNDFETLHIAVDQRGVARLTLNRPEQHNALSGRMIDELTTAALHLADNEAVRIVILTGAGTSFCAGGDLGWMREQVNATR
AQRIEEARKLALMLKALRDLPKPLIGRINGQAYGGGVGLISVCDAAISISGARFGLTETKLGLIPATISPYVVARIGEAN
ALRTFTSARLFDAEEGRRIGLLHDVVEAERLDAAVEAEIKPYFSTAPAAVAASKRLVHALGAPIDEAVIDMTLTRLADTW
ETPEAAEGIAAFFAKRAPAWKGGD
>Mature_264_residues
MNDFETLHIAVDQRGVARLTLNRPEQHNALSGRMIDELTTAALHLADNEAVRIVILTGAGTSFCAGGDLGWMREQVNATR
AQRIEEARKLALMLKALRDLPKPLIGRINGQAYGGGVGLISVCDAAISISGARFGLTETKLGLIPATISPYVVARIGEAN
ALRTFTSARLFDAEEGRRIGLLHDVVEAERLDAAVEAEIKPYFSTAPAAVAASKRLVHALGAPIDEAVIDMTLTRLADTW
ETPEAAEGIAAFFAKRAPAWKGGD

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=272, Percent_Identity=28.3088235294118, Blast_Score=92, Evalue=6e-19,
Organism=Homo sapiens, GI4502327, Length=274, Percent_Identity=30.2919708029197, Blast_Score=90, Evalue=2e-18,
Organism=Homo sapiens, GI31542718, Length=253, Percent_Identity=26.8774703557312, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI45643119, Length=259, Percent_Identity=26.2548262548263, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI260275230, Length=259, Percent_Identity=26.2548262548263, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI260274832, Length=259, Percent_Identity=26.2548262548263, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI116235487, Length=234, Percent_Identity=24.3589743589744, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI157694516, Length=255, Percent_Identity=24.7058823529412, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI213417737, Length=255, Percent_Identity=24.7058823529412, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI70995211, Length=226, Percent_Identity=26.9911504424779, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI37594471, Length=196, Percent_Identity=28.0612244897959, Blast_Score=66, Evalue=2e-11,
Organism=Homo sapiens, GI37594469, Length=175, Percent_Identity=29.1428571428571, Blast_Score=66, Evalue=3e-11,
Organism=Escherichia coli, GI1787659, Length=261, Percent_Identity=29.5019157088123, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI221142681, Length=264, Percent_Identity=29.9242424242424, Blast_Score=91, Evalue=6e-20,
Organism=Escherichia coli, GI1788597, Length=262, Percent_Identity=28.2442748091603, Blast_Score=90, Evalue=1e-19,
Organism=Escherichia coli, GI1787660, Length=269, Percent_Identity=29.7397769516729, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI1790281, Length=188, Percent_Identity=27.1276595744681, Blast_Score=68, Evalue=5e-13,
Organism=Escherichia coli, GI1788682, Length=217, Percent_Identity=28.110599078341, Blast_Score=65, Evalue=3e-12,
Organism=Escherichia coli, GI87082183, Length=253, Percent_Identity=24.901185770751, Blast_Score=64, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI17535521, Length=256, Percent_Identity=26.171875, Blast_Score=91, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI25145438, Length=247, Percent_Identity=27.5303643724696, Blast_Score=86, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17554946, Length=270, Percent_Identity=27.037037037037, Blast_Score=84, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI17549921, Length=209, Percent_Identity=31.5789473684211, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17540714, Length=222, Percent_Identity=30.1801801801802, Blast_Score=83, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI25141363, Length=172, Percent_Identity=28.4883720930233, Blast_Score=78, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI17536985, Length=194, Percent_Identity=29.3814432989691, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17540306, Length=247, Percent_Identity=27.5303643724696, Blast_Score=75, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6320241, Length=221, Percent_Identity=26.2443438914027, Blast_Score=65, Evalue=9e-12,
Organism=Drosophila melanogaster, GI24653139, Length=257, Percent_Identity=29.5719844357977, Blast_Score=99, Evalue=3e-21,
Organism=Drosophila melanogaster, GI19922422, Length=255, Percent_Identity=26.2745098039216, Blast_Score=96, Evalue=3e-20,
Organism=Drosophila melanogaster, GI20129971, Length=193, Percent_Identity=31.6062176165803, Blast_Score=92, Evalue=5e-19,
Organism=Drosophila melanogaster, GI24653477, Length=193, Percent_Identity=31.6062176165803, Blast_Score=92, Evalue=5e-19,
Organism=Drosophila melanogaster, GI24654903, Length=261, Percent_Identity=24.904214559387, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24650670, Length=175, Percent_Identity=32.5714285714286, Blast_Score=68, Evalue=6e-12,
Organism=Drosophila melanogaster, GI19920382, Length=238, Percent_Identity=27.7310924369748, Blast_Score=67, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 28207; Mature: 28207

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDFETLHIAVDQRGVARLTLNRPEQHNALSGRMIDELTTAALHLADNEAVRIVILTGAG
CCCCEEEEEEECCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHEECCCCEEEEEEEECCC
TSFCAGGDLGWMREQVNATRAQRIEEARKLALMLKALRDLPKPLIGRINGQAYGGGVGLI
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCCCHHH
SVCDAAISISGARFGLTETKLGLIPATISPYVVARIGEANALRTFTSARLFDAEEGRRIG
HHHHHHHCCCCCCCCCCCCCCCEECCCCCCEEEEECCCCHHHHHHHHHHEEECCCCCEEH
LLHDVVEAERLDAAVEAEIKPYFSTAPAAVAASKRLVHALGAPIDEAVIDMTLTRLADTW
HHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCC
ETPEAAEGIAAFFAKRAPAWKGGD
CCCHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MNDFETLHIAVDQRGVARLTLNRPEQHNALSGRMIDELTTAALHLADNEAVRIVILTGAG
CCCCEEEEEEECCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHEECCCCEEEEEEEECCC
TSFCAGGDLGWMREQVNATRAQRIEEARKLALMLKALRDLPKPLIGRINGQAYGGGVGLI
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCCCHHH
SVCDAAISISGARFGLTETKLGLIPATISPYVVARIGEANALRTFTSARLFDAEEGRRIG
HHHHHHHCCCCCCCCCCCCCCCEECCCCCCEEEEECCCCHHHHHHHHHHEEECCCCCEEH
LLHDVVEAERLDAAVEAEIKPYFSTAPAAVAASKRLVHALGAPIDEAVIDMTLTRLADTW
HHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCC
ETPEAAEGIAAFFAKRAPAWKGGD
CCCHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA