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The map label for this gene is livJ [C]

Identifier: 23500930

GI number: 23500930

Start: 16003

End: 17109

Strand: Reverse

Name: livJ [C]

Synonym: BR0014

Alternate gene names: 23500930

Gene position: 17109-16003 (Counterclockwise)

Preceding gene: 23500932

Following gene: 23500929

Centisome position: 0.81

GC content: 59.26

Gene sequence:

>1107_bases
ATGAACCTGAAACTTCTGTCCAGCTTGGCTTTTGCCGCTACCATCGGCTTTGCCAGCGCCGCTTACGCAGACATCACCAT
CGGTGTGATTGCACCGCTGACGGGCCCGGTTGCCGCTTTCGGCGATCAGGTGAAAAAGGGCGCGGAAACCGCCGTGGAAG
TCATCAACAAGGCTGGCGGCATCAAGGGTGAGAAAGTCGTCCTGAAGTTTGCCGACGATGCGGGCGAGCCGAAGCAGGGC
GTTTCCGCTGCCAACCAGATCGTTGGCGATGGCATCAAGTTTGTCGTCGGCCCGGTTACGACCGGCGTTGCCGTCCCCGT
TTCCGACGTTTTGTCTGAAAACGGCGTCCTCATGGTCACCCCGACGGCTACCGGCCCGGACCTGACCGCGCGCGGCCTGG
AAAACGTGTTCCGTACCTGCGGCCGCGACGACCAGCAGGCTGAAGTCATGGCTGATTACGTCCTGAAAAACATGAAGGAC
AAGAAGGTTGCCGTGATCCACGACAAGGGCGCTTATGGCAAGGGTCTTGCCGATGCGTTCAAGGCGGCCATCAACAAGGG
CGGCATCACTGAAGTCCATTATGATTCGGTCACACCGGGCGACAAGGATTTTTCCGCGCTCGTCACCAAGCTGAAGTCTG
CCGGTGCTGAAGTGGTCTATTTCGGCGGCTACCATGCTGAAGGCGGTCTCCTGTCGCGCCAGCTCCACGATGCTGGCATG
CAGGCCCTGGTGCTCGGCGGCGAAGGCCTCTCCAACACCGAATATTGGGCAATCGGCGGCACAAACGCACAGGGTACGCT
CTTCACCAACGCCAAGGATGCCACGAAGAACCCGGCTGCCAAGGACGCTATCCAAGCCCTGAAGGCGAAGAACATCCCGG
CTGAAGCTTTCACGATGAACGCCTATGCGGCTGTGGAAGTCATCAAGGCCGGCATCGAACGCGCCGGTTCGACTGACGAT
TCCGCTGCCGTTGCCAAGGCGCTGCATGACGGCAAGCCGATCGAAACCGCAATCGGCACGCTGACCTATAGCGAAACCGG
CGACCTTAGCTCGCCAAGCTTTGATATCTTCAAGTGGGATGACGGCAAGATCGTCGGTCTTGAATAA

Upstream 100 bases:

>100_bases
AGGGCCCGGCAATTCGGCAGCGCGCAGGCGCTGAAAATTGACCGGCATGGGTTGTTTCGGGGCTGTGCGGACAATGCCGC
CCCACCAAAGGAGAAAAAGA

Downstream 100 bases:

>100_bases
GTTTTACTGACTTGACGTGATATGAAATGCCGGGGCGGGAAAACGCCCCGGCATTTTGTATTTCCCGGCTCCATAAAAAT
AAAGCATGTCAAATTTTATA

Product: branched-chain amino acid ABC transporter perisplasmic amino acid-binding protein

Products: ADP; phosphate; L-leucine [Cytoplasm]; ADP; L-valine [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 368; Mature: 368

Protein sequence:

>368_residues
MNLKLLSSLAFAATIGFASAAYADITIGVIAPLTGPVAAFGDQVKKGAETAVEVINKAGGIKGEKVVLKFADDAGEPKQG
VSAANQIVGDGIKFVVGPVTTGVAVPVSDVLSENGVLMVTPTATGPDLTARGLENVFRTCGRDDQQAEVMADYVLKNMKD
KKVAVIHDKGAYGKGLADAFKAAINKGGITEVHYDSVTPGDKDFSALVTKLKSAGAEVVYFGGYHAEGGLLSRQLHDAGM
QALVLGGEGLSNTEYWAIGGTNAQGTLFTNAKDATKNPAAKDAIQALKAKNIPAEAFTMNAYAAVEVIKAGIERAGSTDD
SAAVAKALHDGKPIETAIGTLTYSETGDLSSPSFDIFKWDDGKIVGLE

Sequences:

>Translated_368_residues
MNLKLLSSLAFAATIGFASAAYADITIGVIAPLTGPVAAFGDQVKKGAETAVEVINKAGGIKGEKVVLKFADDAGEPKQG
VSAANQIVGDGIKFVVGPVTTGVAVPVSDVLSENGVLMVTPTATGPDLTARGLENVFRTCGRDDQQAEVMADYVLKNMKD
KKVAVIHDKGAYGKGLADAFKAAINKGGITEVHYDSVTPGDKDFSALVTKLKSAGAEVVYFGGYHAEGGLLSRQLHDAGM
QALVLGGEGLSNTEYWAIGGTNAQGTLFTNAKDATKNPAAKDAIQALKAKNIPAEAFTMNAYAAVEVIKAGIERAGSTDD
SAAVAKALHDGKPIETAIGTLTYSETGDLSSPSFDIFKWDDGKIVGLE
>Mature_368_residues
MNLKLLSSLAFAATIGFASAAYADITIGVIAPLTGPVAAFGDQVKKGAETAVEVINKAGGIKGEKVVLKFADDAGEPKQG
VSAANQIVGDGIKFVVGPVTTGVAVPVSDVLSENGVLMVTPTATGPDLTARGLENVFRTCGRDDQQAEVMADYVLKNMKD
KKVAVIHDKGAYGKGLADAFKAAINKGGITEVHYDSVTPGDKDFSALVTKLKSAGAEVVYFGGYHAEGGLLSRQLHDAGM
QALVLGGEGLSNTEYWAIGGTNAQGTLFTNAKDATKNPAAKDAIQALKAKNIPAEAFTMNAYAAVEVIKAGIERAGSTDD
SAAVAKALHDGKPIETAIGTLTYSETGDLSSPSFDIFKWDDGKIVGLE

Specific function: Component of an amino-acid transport system (Potential) [H]

COG id: COG0683

COG function: function code E; ABC-type branched-chain amino acid transport systems, periplasmic component

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI48994941, Length=361, Percent_Identity=41.8282548476454, Blast_Score=283, Evalue=1e-77,
Organism=Escherichia coli, GI1789867, Length=337, Percent_Identity=39.7626112759644, Blast_Score=254, Evalue=6e-69,

Paralogues:

None

Copy number: 10140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 8822 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal med

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001828
- InterPro:   IPR000709 [H]

Pfam domain/function: PF01094 ANF_receptor [H]

EC number: NA

Molecular weight: Translated: 37940; Mature: 37940

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLKLLSSLAFAATIGFASAAYADITIGVIAPLTGPVAAFGDQVKKGAETAVEVINKAGG
CCHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCC
IKGEKVVLKFADDAGEPKQGVSAANQIVGDGIKFVVGPVTTGVAVPVSDVLSENGVLMVT
CCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCEEEECCCCCCCCCCHHHHHCCCCEEEEE
PTATGPDLTARGLENVFRTCGRDDQQAEVMADYVLKNMKDKKVAVIHDKGAYGKGLADAF
CCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHH
KAAINKGGITEVHYDSVTPGDKDFSALVTKLKSAGAEVVYFGGYHAEGGLLSRQLHDAGM
HHHHCCCCCCEEEECCCCCCCCHHHHHHHHHHHCCCCEEEECCEECCCCHHHHHHHHCCC
QALVLGGEGLSNTEYWAIGGTNAQGTLFTNAKDATKNPAAKDAIQALKAKNIPAEAFTMN
EEEEECCCCCCCCEEEEEECCCCCCEEEECCCCCCCCCHHHHHHHHHHHCCCCCHHEECH
AYAAVEVIKAGIERAGSTDDSAAVAKALHDGKPIETAIGTLTYSETGDLSSPSFDIFKWD
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHHEEEECCCCCCCCCCCEEEEEC
DGKIVGLE
CCEEEECC
>Mature Secondary Structure
MNLKLLSSLAFAATIGFASAAYADITIGVIAPLTGPVAAFGDQVKKGAETAVEVINKAGG
CCHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCC
IKGEKVVLKFADDAGEPKQGVSAANQIVGDGIKFVVGPVTTGVAVPVSDVLSENGVLMVT
CCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCEEEECCCCCCCCCCHHHHHCCCCEEEEE
PTATGPDLTARGLENVFRTCGRDDQQAEVMADYVLKNMKDKKVAVIHDKGAYGKGLADAF
CCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHH
KAAINKGGITEVHYDSVTPGDKDFSALVTKLKSAGAEVVYFGGYHAEGGLLSRQLHDAGM
HHHHCCCCCCEEEECCCCCCCCHHHHHHHHHHHCCCCEEEECCEECCCCHHHHHHHHCCC
QALVLGGEGLSNTEYWAIGGTNAQGTLFTNAKDATKNPAAKDAIQALKAKNIPAEAFTMN
EEEEECCCCCCCCEEEEEECCCCCCEEEECCCCCCCCCHHHHHHHHHHHCCCCCHHEECH
AYAAVEVIKAGIERAGSTDDSAAVAKALHDGKPIETAIGTLTYSETGDLSSPSFDIFKWD
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHHEEEECCCCCCCCCCCEEEEEC
DGKIVGLE
CCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O; ATP; L-valine [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11756688 [H]