Click here to switch to the map view.

The map label for this gene is malK [H]

Identifier: 23100675

GI number: 23100675

Start: 3352559

End: 3354160

Strand: Reverse

Name: malK [H]

Synonym: OB3220

Alternate gene names: 23100675

Gene position: 3354160-3352559 (Counterclockwise)

Preceding gene: 23100676

Following gene: 23100674

Centisome position: 92.39

GC content: 35.96

Gene sequence:

>1602_bases
ATGGGTAAGAAAAAAACACGTCTAAGCATATTGATAACAACGCTTGTTTGTTTAGTAGTTATTATTGTTCTTATTACCAA
TGATTTATTAATAAATTATGCAACCGGTGAACGGCTAAAAGAAAATATTGAGGAAAAGGCAGTAATTATTTCTCGAACAA
TGGCCAAGTCCGAATGGGTAATTAATGGGCTACAAAATAAAGATGAAGAGAAATATATACAAGAATATACGAATGAAATA
AGTCGCTATACGGATTTAACATTTATTGTAGTTATGGATATGGATGGTATACGTAAGTCACATCCGAACCCGGAGTTAAT
TGGAAAGCCTTTTGTAGGAGGGGATGAAGATACCGTATTAGAACAAGGTATCGAGCATATTTCCATATCTGAGGGGACGC
TTGGTGACTCATTGCGCGCTTTCTCCCCAATATTTAATGAAGAGGGCGAACAAATTGGTGCAGTGTCGGTGGGGATATCC
CTACAAGAAATTGATGATGTCATCAATAACAATCATATCAGCAATCTAATTAGCTCGATTGTTGGTTTAATTATTGGCAT
CTTAGGAGCATGGTTTATTGCCCGTTATATTAAGCGGATTTTATTTGGTTTAGAGCCGTTTCAAATTGCTAAATTGCTAA
AAGAAAGAAGCGCTACGTTAGAGTCAGCAAGAGAAGGCATCATAACCATCGATAAGGACCATAAAATAACGATTGTAAAT
AAAGCCGCAATGAAAGTTTTTGAAAAAGCAGGAATTACCGAATCCCCTGTTGGTAAAAACGTAGAAGAGGTTCTTCCTCA
CTCGCATTTAGAGGATATTTTAAAAAGCAAACAGCCCGAGATGGATGTTGAACAGAAAATAAACGGCGTGACTATTTTGG
CAAATCGAATGCCTATTATAGTAAATGATGAAGTGGTAGGTGCTATTTCCACTTTTAGAGATTTAACAGAACTAAAACAA
TTAGCCAAACAATTATCAGGGGTAAAGCTGTATGCCGAGGCATTACGTGCACAGTCTCATGAATTTATGAACAAATTGCA
TGTGATTAATGGCATGGTAACGACAAAAAGTTACGATGAATTAGACGATTATATTAAACAAATTGTTAATCAGCGTAATG
ATGAGCTAAATCATGTTGTCAAAAATGTAAAAGACTCGATATTGGCAGGGTTTCTCCTAGGTAAATTAAGCGATGCGAGA
GAAAGAGATGTTAATCTGCAAATTGATGTCGCAGAAGAAATACCTGCATCTGCCGATAATCAAGTTGTCCATAAATTAAT
TACCATTATTGGAAATCTCACAGATAATGCAATTGATGCAATAAAATCGAAAGAAAACCGTACAATCGATATGTATGTAA
GCTATAAGAATGATTGGCTCTATATAGGGATTCAAGATTATGGTATTGGCATAGATGAACATATACAGCAACAGATGTTT
CAAAAAGGCTACTCCACCAAAGGAAATAACCGGGGCTATGGTTTATATTTAGTAAGACAGAGTGTAGAGACGTTGGGTGG
ATCCTTGCGTTTTGAAACACTTGAAGCTGGAACTATTTTTGAAGTCGAAGTTCCTTATCAGATAAGGAGAGATATACAAT
GA

Upstream 100 bases:

>100_bases
TACAATCAGTGGAAGGAACCGACGCTAATTTGCTAACATGTCTCGCTAAATTAATTTTCTGTGGTAGGATAAAGTGACAA
TAATCTTGGAGGAAATATCG

Downstream 100 bases:

>100_bases
TTCGGGTACTAATTGTAGAAGATGATCCAATGGTAGCGGAATTTAATAAAAGATATATACAGCTTATCGATGGATTCGAA
GTAACAGCGATCGCAACCAA

Product: sensory histidine kinase DcuS

Products: NA

Alternate protein names: Malate kinase sensor [H]

Number of amino acids: Translated: 533; Mature: 532

Protein sequence:

>533_residues
MGKKKTRLSILITTLVCLVVIIVLITNDLLINYATGERLKENIEEKAVIISRTMAKSEWVINGLQNKDEEKYIQEYTNEI
SRYTDLTFIVVMDMDGIRKSHPNPELIGKPFVGGDEDTVLEQGIEHISISEGTLGDSLRAFSPIFNEEGEQIGAVSVGIS
LQEIDDVINNNHISNLISSIVGLIIGILGAWFIARYIKRILFGLEPFQIAKLLKERSATLESAREGIITIDKDHKITIVN
KAAMKVFEKAGITESPVGKNVEEVLPHSHLEDILKSKQPEMDVEQKINGVTILANRMPIIVNDEVVGAISTFRDLTELKQ
LAKQLSGVKLYAEALRAQSHEFMNKLHVINGMVTTKSYDELDDYIKQIVNQRNDELNHVVKNVKDSILAGFLLGKLSDAR
ERDVNLQIDVAEEIPASADNQVVHKLITIIGNLTDNAIDAIKSKENRTIDMYVSYKNDWLYIGIQDYGIGIDEHIQQQMF
QKGYSTKGNNRGYGLYLVRQSVETLGGSLRFETLEAGTIFEVEVPYQIRRDIQ

Sequences:

>Translated_533_residues
MGKKKTRLSILITTLVCLVVIIVLITNDLLINYATGERLKENIEEKAVIISRTMAKSEWVINGLQNKDEEKYIQEYTNEI
SRYTDLTFIVVMDMDGIRKSHPNPELIGKPFVGGDEDTVLEQGIEHISISEGTLGDSLRAFSPIFNEEGEQIGAVSVGIS
LQEIDDVINNNHISNLISSIVGLIIGILGAWFIARYIKRILFGLEPFQIAKLLKERSATLESAREGIITIDKDHKITIVN
KAAMKVFEKAGITESPVGKNVEEVLPHSHLEDILKSKQPEMDVEQKINGVTILANRMPIIVNDEVVGAISTFRDLTELKQ
LAKQLSGVKLYAEALRAQSHEFMNKLHVINGMVTTKSYDELDDYIKQIVNQRNDELNHVVKNVKDSILAGFLLGKLSDAR
ERDVNLQIDVAEEIPASADNQVVHKLITIIGNLTDNAIDAIKSKENRTIDMYVSYKNDWLYIGIQDYGIGIDEHIQQQMF
QKGYSTKGNNRGYGLYLVRQSVETLGGSLRFETLEAGTIFEVEVPYQIRRDIQ
>Mature_532_residues
GKKKTRLSILITTLVCLVVIIVLITNDLLINYATGERLKENIEEKAVIISRTMAKSEWVINGLQNKDEEKYIQEYTNEIS
RYTDLTFIVVMDMDGIRKSHPNPELIGKPFVGGDEDTVLEQGIEHISISEGTLGDSLRAFSPIFNEEGEQIGAVSVGISL
QEIDDVINNNHISNLISSIVGLIIGILGAWFIARYIKRILFGLEPFQIAKLLKERSATLESAREGIITIDKDHKITIVNK
AAMKVFEKAGITESPVGKNVEEVLPHSHLEDILKSKQPEMDVEQKINGVTILANRMPIIVNDEVVGAISTFRDLTELKQL
AKQLSGVKLYAEALRAQSHEFMNKLHVINGMVTTKSYDELDDYIKQIVNQRNDELNHVVKNVKDSILAGFLLGKLSDARE
RDVNLQIDVAEEIPASADNQVVHKLITIIGNLTDNAIDAIKSKENRTIDMYVSYKNDWLYIGIQDYGIGIDEHIQQQMFQ
KGYSTKGNNRGYGLYLVRQSVETLGGSLRFETLEAGTIFEVEVPYQIRRDIQ

Specific function: Member of a two-component regulatory system malK/malR. Involved in the activation of maeA, maeN and yflS in presence of malate. Probably activates malR by phosphorylation [H]

COG id: COG3290

COG function: function code T; Signal transduction histidine kinase regulating citrate/malate metabolism

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1790567, Length=534, Percent_Identity=36.1423220973783, Blast_Score=348, Evalue=4e-97,
Organism=Escherichia coli, GI1786837, Length=535, Percent_Identity=29.1588785046729, Blast_Score=241, Evalue=7e-65,
Organism=Escherichia coli, GI1788549, Length=337, Percent_Identity=22.2551928783383, Blast_Score=72, Evalue=8e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR016120
- InterPro:   IPR005467
- InterPro:   IPR016121 [H]

Pfam domain/function: PF02518 HATPase_c; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 59853; Mature: 59721

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKKKTRLSILITTLVCLVVIIVLITNDLLINYATGERLKENIEEKAVIISRTMAKSEWV
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHH
INGLQNKDEEKYIQEYTNEISRYTDLTFIVVMDMDGIRKSHPNPELIGKPFVGGDEDTVL
HCCCCCCCHHHHHHHHHHHHHHHCCEEEEEEECCCCHHCCCCCCCCCCCCCCCCCCHHHH
EQGIEHISISEGTLGDSLRAFSPIFNEEGEQIGAVSVGISLQEIDDVINNNHISNLISSI
HHCCCEEECCCCCCCCHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHCCHHHHHHHHHH
VGLIIGILGAWFIARYIKRILFGLEPFQIAKLLKERSATLESAREGIITIDKDHKITIVN
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCEEECCCCEEEEEE
KAAMKVFEKAGITESPVGKNVEEVLPHSHLEDILKSKQPEMDVEQKINGVTILANRMPII
HHHHHHHHHCCCCCCCCCCCHHHHCCHHHHHHHHHCCCCCCCHHHCCCCEEEEECCCCEE
VNDEVVGAISTFRDLTELKQLAKQLSGVKLYAEALRAQSHEFMNKLHVINGMVTTKSYDE
ECCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCEECCCCHHH
LDDYIKQIVNQRNDELNHVVKNVKDSILAGFLLGKLSDARERDVNLQIDVAEEIPASADN
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCEEEEEHHHCCCCCCH
QVVHKLITIIGNLTDNAIDAIKSKENRTIDMYVSYKNDWLYIGIQDYGIGIDEHIQQQMF
HHHHHHHHHHHCCCHHHHHHHHCCCCCEEEEEEEECCCEEEEEEEHHCCCHHHHHHHHHH
QKGYSTKGNNRGYGLYLVRQSVETLGGSLRFETLEAGTIFEVEVPYQIRRDIQ
HCCCCCCCCCCCEEEEEEHHHHHHHCCCEEEEEECCCEEEEEECCHHHHCCCC
>Mature Secondary Structure 
GKKKTRLSILITTLVCLVVIIVLITNDLLINYATGERLKENIEEKAVIISRTMAKSEWV
CCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHH
INGLQNKDEEKYIQEYTNEISRYTDLTFIVVMDMDGIRKSHPNPELIGKPFVGGDEDTVL
HCCCCCCCHHHHHHHHHHHHHHHCCEEEEEEECCCCHHCCCCCCCCCCCCCCCCCCHHHH
EQGIEHISISEGTLGDSLRAFSPIFNEEGEQIGAVSVGISLQEIDDVINNNHISNLISSI
HHCCCEEECCCCCCCCHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHCCHHHHHHHHHH
VGLIIGILGAWFIARYIKRILFGLEPFQIAKLLKERSATLESAREGIITIDKDHKITIVN
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCEEECCCCEEEEEE
KAAMKVFEKAGITESPVGKNVEEVLPHSHLEDILKSKQPEMDVEQKINGVTILANRMPII
HHHHHHHHHCCCCCCCCCCCHHHHCCHHHHHHHHHCCCCCCCHHHCCCCEEEEECCCCEE
VNDEVVGAISTFRDLTELKQLAKQLSGVKLYAEALRAQSHEFMNKLHVINGMVTTKSYDE
ECCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCEECCCCHHH
LDDYIKQIVNQRNDELNHVVKNVKDSILAGFLLGKLSDARERDVNLQIDVAEEIPASADN
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCEEEEEHHHCCCCCCH
QVVHKLITIIGNLTDNAIDAIKSKENRTIDMYVSYKNDWLYIGIQDYGIGIDEHIQQQMF
HHHHHHHHHHHCCCHHHHHHHHCCCCCEEEEEEEECCCEEEEEEEHHCCCHHHHHHHHHH
QKGYSTKGNNRGYGLYLVRQSVETLGGSLRFETLEAGTIFEVEVPYQIRRDIQ
HCCCCCCCCCCCEEEEEEHHHHHHHCCCEEEEEECCCEEEEEECCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9274030; 9384377; 12949159; 12949160 [H]