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The map label for this gene is sseA [H]

Identifier: 23100648

GI number: 23100648

Start: 3323887

End: 3324783

Strand: Direct

Name: sseA [H]

Synonym: OB3193

Alternate gene names: 23100648

Gene position: 3323887-3324783 (Clockwise)

Preceding gene: 23100647

Following gene: 23100649

Centisome position: 91.55

GC content: 40.91

Gene sequence:

>897_bases
ATGAGCGAGCGCAATATTCCCTTATTGGTAACTACTGATTGGTTAGCTGAGAGAATGGATGATCCTAACCTACGCATCTT
GGATGCAACGACATTTTTAAAACAACCAGCACAAGGCGGCTATTATGATGTATGGTCAGGTAAAGACGTATATGATAAAG
GACATATTCCAGGTGCCGTGTATGCAGACCTTCTAGGAGAACTATCTGACGCTGACTCTCCTTTTAAATTTACGGTTCCA
CACCGAGAACAATTTATTGAAAAAATCACCGATCTTGGAGTTGGTGATGATACTTTTGTCGTTGTCTATGACCAAGCACA
AGCAGTTGTTGGCGCGCCAATTATCGGTTCAGACTGGGCATCCAGACTTGCATGGCAATTACGCTATGAAGGTTTTGAAA
ATGTAGCAGTTCTTGATGGCGGCTTCTTAAAGTGGGAAAAAGAAGATCGTCCTATCTCTACAGAAGCAAAACCATACTCG
AAAGGTCATTTTACTGGCACACGTAACGATGACTTATTAGCGACAAAAGAAGATGTAAAAAATGCAATGGAAGATGATAA
TGTTGTACTTATCAACAGTTTATCCCCAGCTGATTTTGCTGGCGAATCTGATACGTATGAACGTTCCGGTCATATTCCTA
GCAGTGTCAATGTTTTCTTCGGACAACACAGTGACCAAGATACACTACAATTACATCCGGAAGAACAATTGCGAGAAACA
TTTGAAAAAGTTGGAGCACTAGACCCCAACAAAAAAGTCATTACCTACTGTGGTAGCGGAATTGCGGCTACGTGGAACGC
ATTAATTCTCAATCAATTAGGCCAAGATAATGTAGCTATGTATGATGGTTCCATGACAGAATGGGCCAATGACAAAGACC
TTCCATTAAATAAATAG

Upstream 100 bases:

>100_bases
AAAGCTAAATTTTAGCAAAAGAGATAAACATCTTTTATACTATTACTAAATACAAGTAAATAGATAAGAATTATTATATA
TCAGAAAGGAAGATAAACAA

Downstream 100 bases:

>100_bases
AGTTACTATCCAAACAAGTAGAAGCAACCATAGCTTCTACTTGTTTTTTTATGAAAAATTTTAGTTGTACAAGCTGTTAG
ATCGAATAGAATAGATATAG

Product: thiosulfate sulfurtransferase

Products: NA

Alternate protein names: MST; Rhodanese-like protein [H]

Number of amino acids: Translated: 298; Mature: 297

Protein sequence:

>298_residues
MSERNIPLLVTTDWLAERMDDPNLRILDATTFLKQPAQGGYYDVWSGKDVYDKGHIPGAVYADLLGELSDADSPFKFTVP
HREQFIEKITDLGVGDDTFVVVYDQAQAVVGAPIIGSDWASRLAWQLRYEGFENVAVLDGGFLKWEKEDRPISTEAKPYS
KGHFTGTRNDDLLATKEDVKNAMEDDNVVLINSLSPADFAGESDTYERSGHIPSSVNVFFGQHSDQDTLQLHPEEQLRET
FEKVGALDPNKKVITYCGSGIAATWNALILNQLGQDNVAMYDGSMTEWANDKDLPLNK

Sequences:

>Translated_298_residues
MSERNIPLLVTTDWLAERMDDPNLRILDATTFLKQPAQGGYYDVWSGKDVYDKGHIPGAVYADLLGELSDADSPFKFTVP
HREQFIEKITDLGVGDDTFVVVYDQAQAVVGAPIIGSDWASRLAWQLRYEGFENVAVLDGGFLKWEKEDRPISTEAKPYS
KGHFTGTRNDDLLATKEDVKNAMEDDNVVLINSLSPADFAGESDTYERSGHIPSSVNVFFGQHSDQDTLQLHPEEQLRET
FEKVGALDPNKKVITYCGSGIAATWNALILNQLGQDNVAMYDGSMTEWANDKDLPLNK
>Mature_297_residues
SERNIPLLVTTDWLAERMDDPNLRILDATTFLKQPAQGGYYDVWSGKDVYDKGHIPGAVYADLLGELSDADSPFKFTVPH
REQFIEKITDLGVGDDTFVVVYDQAQAVVGAPIIGSDWASRLAWQLRYEGFENVAVLDGGFLKWEKEDRPISTEAKPYSK
GHFTGTRNDDLLATKEDVKNAMEDDNVVLINSLSPADFAGESDTYERSGHIPSSVNVFFGQHSDQDTLQLHPEEQLRETF
EKVGALDPNKKVITYCGSGIAATWNALILNQLGQDNVAMYDGSMTEWANDKDLPLNK

Specific function: Transfers a sulfur ion to cyanide or to other thiol compounds. Also has weak rhodanese activity. Its participation in detoxification of cyanide may be small. May be involved in the enhancement of serine sensitivity [H]

COG id: COG2897

COG function: function code P; Rhodanese-related sulfurtransferase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 rhodanese domains [H]

Homologues:

Organism=Homo sapiens, GI17402865, Length=288, Percent_Identity=33.6805555555556, Blast_Score=138, Evalue=6e-33,
Organism=Homo sapiens, GI61835204, Length=288, Percent_Identity=31.25, Blast_Score=135, Evalue=5e-32,
Organism=Homo sapiens, GI194473681, Length=288, Percent_Identity=31.25, Blast_Score=135, Evalue=5e-32,
Organism=Homo sapiens, GI194473668, Length=288, Percent_Identity=31.25, Blast_Score=135, Evalue=5e-32,
Organism=Escherichia coli, GI87082121, Length=291, Percent_Identity=32.9896907216495, Blast_Score=135, Evalue=3e-33,
Organism=Escherichia coli, GI87081967, Length=266, Percent_Identity=23.3082706766917, Blast_Score=70, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17561888, Length=300, Percent_Identity=24.3333333333333, Blast_Score=68, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI115534702, Length=256, Percent_Identity=26.953125, Blast_Score=66, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6324825, Length=261, Percent_Identity=28.3524904214559, Blast_Score=87, Evalue=4e-18,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001763
- InterPro:   IPR001307 [H]

Pfam domain/function: PF00581 Rhodanese [H]

EC number: =2.8.1.2 [H]

Molecular weight: Translated: 33184; Mature: 33053

Theoretical pI: Translated: 4.23; Mature: 4.23

Prosite motif: PS50206 RHODANESE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSERNIPLLVTTDWLAERMDDPNLRILDATTFLKQPAQGGYYDVWSGKDVYDKGHIPGAV
CCCCCCCEEEECHHHHHHCCCCCEEEEEHHHHHHCCCCCCEEECCCCCCCCCCCCCCHHH
YADLLGELSDADSPFKFTVPHREQFIEKITDLGVGDDTFVVVYDQAQAVVGAPIIGSDWA
HHHHHHHHCCCCCCEEEECCHHHHHHHHHHHCCCCCCEEEEEECCCCCEECCCCCCCHHH
SRLAWQLRYEGFENVAVLDGGFLKWEKEDRPISTEAKPYSKGHFTGTRNDDLLATKEDVK
HHHHHHHHHCCCCCEEEECCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCEECHHHHH
NAMEDDNVVLINSLSPADFAGESDTYERSGHIPSSVNVFFGQHSDQDTLQLHPEEQLRET
HHHCCCCEEEEECCCCCCCCCCCCCCHHCCCCCCCEEEEECCCCCCCCEEECCHHHHHHH
FEKVGALDPNKKVITYCGSGIAATWNALILNQLGQDNVAMYDGSMTEWANDKDLPLNK
HHHHCCCCCCCEEEEEECCCHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SERNIPLLVTTDWLAERMDDPNLRILDATTFLKQPAQGGYYDVWSGKDVYDKGHIPGAV
CCCCCCEEEECHHHHHHCCCCCEEEEEHHHHHHCCCCCCEEECCCCCCCCCCCCCCHHH
YADLLGELSDADSPFKFTVPHREQFIEKITDLGVGDDTFVVVYDQAQAVVGAPIIGSDWA
HHHHHHHHCCCCCCEEEECCHHHHHHHHHHHCCCCCCEEEEEECCCCCEECCCCCCCHHH
SRLAWQLRYEGFENVAVLDGGFLKWEKEDRPISTEAKPYSKGHFTGTRNDDLLATKEDVK
HHHHHHHHHCCCCCEEEECCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCEECHHHHH
NAMEDDNVVLINSLSPADFAGESDTYERSGHIPSSVNVFFGQHSDQDTLQLHPEEQLRET
HHHCCCCEEEEECCCCCCCCCCCCCCHHCCCCCCCEEEEECCCCCCCCEEECCHHHHHHH
FEKVGALDPNKKVITYCGSGIAATWNALILNQLGQDNVAMYDGSMTEWANDKDLPLNK
HHHHCCCCCCCEEEEEECCCHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]