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The map label for this gene is potB [H]

Identifier: 23100610

GI number: 23100610

Start: 3285846

End: 3286649

Strand: Direct

Name: potB [H]

Synonym: OB3155

Alternate gene names: 23100610

Gene position: 3285846-3286649 (Clockwise)

Preceding gene: 23100609

Following gene: 23100611

Centisome position: 90.51

GC content: 37.44

Gene sequence:

>804_bases
ATGGAGAATAAGACGCTTCGTAGTTTATACATGCTACCTTATGCACTCTGGATTATCCTCTTCGTCATTGCACCGATTTT
ACTCGTTGTTTATTTCTCATTCTTTGATATTGAGGGTAATTTCTCTTTAGAGAATTATAAGAATTTCTTCACAGCGACCT
ATTTGCAAATGACCTTGAATTCATTTTGGTATGCATTTCTTATCACTTTATTTTCTCTATTAGTTGCTTACCCAACGGCA
TATGTACTGACCCGTACGAAACATAAGCAATTATGGCTTCTACTGATAATTGTTCCTTCATGGATTAATCTACTATTAAA
AGCGTATGCATTTATTGGTATATTCGGCACGCATGGTGCAGCGAACCAATTCCTAGATTGGATCGGAATAGGATCGAGGC
AACTACTTTTCACCGATTTCAGTTTTGTATTTGTCGCTGTATACATTTTTATACCGTTTATGATCTTACCTATTTTTAAT
GCGTTAGATAATTTAAATCCGAGCTTGCTGTACGCATCCAAAGATTTAGGTGCTTCAAAATGGACAACATTTCGAAGAGT
TATTTTCCCATTAACAATAGATGGCGTGAAGTCTGGATGCCAAGTAGTTTTCATTCCTGCGCTATCTTTATTTATGCTTA
CACGATTAATTGCCGGAAATCGTGTGATTACGCTCGGAACAGCAATTGAGCAGCAATTCCTTGTCACCCAAAACTGGGGC
ATGGGAGCTACCATTGCAGTGTTCCTAATCATTATTATGGTAGGATTTATGGCATTAACATCGTTACGAAGGAGAGGTGT
GTAA

Upstream 100 bases:

>100_bases
GGATTGAGATTTCATCCAGAAGATATACATGTTATGCGATTTAATGAGTCCGAAGCAGACTTTGACCGCCGTTTAGATTC
TTATTCGGAGGGAGAAACAG

Downstream 100 bases:

>100_bases
TATGGAGAATAAATCCTCTTCATTCGCTAATATTTTTCTAGTCCTTATATTTTTCATTTTATATGCTCCAATCTTTTATT
TAATCTTTTATTCATTCAAT

Product: spermidine:putrescine ABC transporter permease

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 267; Mature: 267

Protein sequence:

>267_residues
MENKTLRSLYMLPYALWIILFVIAPILLVVYFSFFDIEGNFSLENYKNFFTATYLQMTLNSFWYAFLITLFSLLVAYPTA
YVLTRTKHKQLWLLLIIVPSWINLLLKAYAFIGIFGTHGAANQFLDWIGIGSRQLLFTDFSFVFVAVYIFIPFMILPIFN
ALDNLNPSLLYASKDLGASKWTTFRRVIFPLTIDGVKSGCQVVFIPALSLFMLTRLIAGNRVITLGTAIEQQFLVTQNWG
MGATIAVFLIIIMVGFMALTSLRRRGV

Sequences:

>Translated_267_residues
MENKTLRSLYMLPYALWIILFVIAPILLVVYFSFFDIEGNFSLENYKNFFTATYLQMTLNSFWYAFLITLFSLLVAYPTA
YVLTRTKHKQLWLLLIIVPSWINLLLKAYAFIGIFGTHGAANQFLDWIGIGSRQLLFTDFSFVFVAVYIFIPFMILPIFN
ALDNLNPSLLYASKDLGASKWTTFRRVIFPLTIDGVKSGCQVVFIPALSLFMLTRLIAGNRVITLGTAIEQQFLVTQNWG
MGATIAVFLIIIMVGFMALTSLRRRGV
>Mature_267_residues
MENKTLRSLYMLPYALWIILFVIAPILLVVYFSFFDIEGNFSLENYKNFFTATYLQMTLNSFWYAFLITLFSLLVAYPTA
YVLTRTKHKQLWLLLIIVPSWINLLLKAYAFIGIFGTHGAANQFLDWIGIGSRQLLFTDFSFVFVAVYIFIPFMILPIFN
ALDNLNPSLLYASKDLGASKWTTFRRVIFPLTIDGVKSGCQVVFIPALSLFMLTRLIAGNRVITLGTAIEQQFLVTQNWG
MGATIAVFLIIIMVGFMALTSLRRRGV

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine [H]

COG id: COG1176

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component I

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787369, Length=257, Percent_Identity=36.1867704280156, Blast_Score=163, Evalue=1e-41,
Organism=Escherichia coli, GI1787080, Length=226, Percent_Identity=34.5132743362832, Blast_Score=134, Evalue=6e-33,
Organism=Escherichia coli, GI1787713, Length=248, Percent_Identity=29.0322580645161, Blast_Score=97, Evalue=9e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 30477; Mature: 30477

Theoretical pI: Translated: 10.01; Mature: 10.01

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENKTLRSLYMLPYALWIILFVIAPILLVVYFSFFDIEGNFSLENYKNFFTATYLQMTLN
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHH
SFWYAFLITLFSLLVAYPTAYVLTRTKHKQLWLLLIIVPSWINLLLKAYAFIGIFGTHGA
HHHHHHHHHHHHHHHHCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
ANQFLDWIGIGSRQLLFTDFSFVFVAVYIFIPFMILPIFNALDNLNPSLLYASKDLGASK
HHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCHH
WTTFRRVIFPLTIDGVKSGCQVVFIPALSLFMLTRLIAGNRVITLGTAIEQQFLVTQNWG
HHHHHHHHHHEEECCCCCCCEEEEHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHEECCCC
MGATIAVFLIIIMVGFMALTSLRRRGV
CCHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MENKTLRSLYMLPYALWIILFVIAPILLVVYFSFFDIEGNFSLENYKNFFTATYLQMTLN
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHH
SFWYAFLITLFSLLVAYPTAYVLTRTKHKQLWLLLIIVPSWINLLLKAYAFIGIFGTHGA
HHHHHHHHHHHHHHHHCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
ANQFLDWIGIGSRQLLFTDFSFVFVAVYIFIPFMILPIFNALDNLNPSLLYASKDLGASK
HHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCHH
WTTFRRVIFPLTIDGVKSGCQVVFIPALSLFMLTRLIAGNRVITLGTAIEQQFLVTQNWG
HHHHHHHHHHEEECCCCCCCEEEEHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHEECCCC
MGATIAVFLIIIMVGFMALTSLRRRGV
CCHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]