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The map label for this gene is argG

Identifier: 23100584

GI number: 23100584

Start: 3262500

End: 3263753

Strand: Reverse

Name: argG

Synonym: OB3129

Alternate gene names: 23100584

Gene position: 3263753-3262500 (Counterclockwise)

Preceding gene: 23100585

Following gene: 23100583

Centisome position: 89.9

GC content: 37.32

Gene sequence:

>1254_bases
ATGACAAAAGAAAAGATTGTATTAGCCTATTCTGGAGGATTAGACACCTCTGTTGCAGTTAAATGGTTACAGGATAAATA
TAATTATGATGTGATTGCAGTTGCATTAGACGTTGGAGAAGGTAAGGATTTAGATTTTGTGAAGAAGAAAGCATTGGATG
TAGGAGCTATCAAATCCTATGTTATTGATGCGAAAAACTTGTTTGCGGAAGAATATGTCTTACCAGCATTACAAGCAAAT
CTATTATATGAAGGAAAGTACCCTTTAATTTCAGCTTTATCTAGACCCCTTATTTCTAAGATTTTAGTAGATATTGCAGA
GGAAGAAGGAGCGGTAGCTGTTGCACATGGCTGCACTGGAAAAGGAAATGATCAAGTGCGCTTTGATGTATCATTTACTG
CACTAAACCCTAACCTTAAGATTGTAGCGCCGGTTCGTGAATGGGCAATGTCAAGAGAAGAAGAAATTGAGTATGCGAAG
TCACATGGGATTCCAATTCCAATTAACTTGGATAGTCCTTACAGTATTGATCAAAATCTATGGGGCAGAAGCAATGAATG
TGGGATTTTAGAAGATCCATGGGCAGAGGCACCTAAAGACGCTTATGATCTAACTATAGATCCAGTAGATGCACCTGAAG
ATCCAGAAGTTATCCAAATTACATTTAAAAAAGGAAAGCCAGTCTCTATTGATGGTAAAGAAATGCCTCTAGATGAACTA
ATTCTACAATTAAATACGATTGCTGGTAAACATGGTGTTGGCAGAATTGATCATGTAGAAAATCGTTTGGTAGGAATTAA
ATCAAGAGAGATTTATGAAGCACCAGCAGCAACGACGTTAATTGCTGCACATCAGGAATTAGAAGCATTAACATTACCAA
GAGAAGTAGCGGAATTCAAGCCGACAATTGAGCAGAAGCTAGCACAAACGGTTTATTATGGATTATGGTATTCACCTTTA
ACAGAAGCACTACAATCTTTTATTGAGAAAACACAGGAACATGTATCTGGAACAGTAAAAGTGAAGTTATATAAAGGTCA
TGCACAAGTAATTGGAAGAGAGTCTGAAAACTCCCTTTATGATTTTGACTTGGCGACATACAACAAAGCAGATGCATTTG
ATCATGATGCTGCATTAGGATTTATTAAACTTTGGGGACTACCTACGCAGGTTCACTCCGCTGTAAATGGACCACAAGGT
AAAAAATCAATTGAAAAAGTAGACTTGGAAGTAAAGGAAGCTGTGAAACCATGA

Upstream 100 bases:

>100_bases
ATAATCAGAAAAAATAAACTTTTTATGAACCTATTGAATTATTATGCATATTAATGCATAATAATTCAATAATAGAACAT
ATCGGAGAGGGGAAGGCAAA

Downstream 100 bases:

>100_bases
AACTATGGGGCGGAAGATTTACAAAACCTACCAACGAATTGGTAGATGAATATGCATCATCCATTCAATTTGATAAAAAA
CTAGCGGCTTATGACATTGA

Product: argininosuccinate synthase

Products: NA

Alternate protein names: Citrulline--aspartate ligase

Number of amino acids: Translated: 417; Mature: 416

Protein sequence:

>417_residues
MTKEKIVLAYSGGLDTSVAVKWLQDKYNYDVIAVALDVGEGKDLDFVKKKALDVGAIKSYVIDAKNLFAEEYVLPALQAN
LLYEGKYPLISALSRPLISKILVDIAEEEGAVAVAHGCTGKGNDQVRFDVSFTALNPNLKIVAPVREWAMSREEEIEYAK
SHGIPIPINLDSPYSIDQNLWGRSNECGILEDPWAEAPKDAYDLTIDPVDAPEDPEVIQITFKKGKPVSIDGKEMPLDEL
ILQLNTIAGKHGVGRIDHVENRLVGIKSREIYEAPAATTLIAAHQELEALTLPREVAEFKPTIEQKLAQTVYYGLWYSPL
TEALQSFIEKTQEHVSGTVKVKLYKGHAQVIGRESENSLYDFDLATYNKADAFDHDAALGFIKLWGLPTQVHSAVNGPQG
KKSIEKVDLEVKEAVKP

Sequences:

>Translated_417_residues
MTKEKIVLAYSGGLDTSVAVKWLQDKYNYDVIAVALDVGEGKDLDFVKKKALDVGAIKSYVIDAKNLFAEEYVLPALQAN
LLYEGKYPLISALSRPLISKILVDIAEEEGAVAVAHGCTGKGNDQVRFDVSFTALNPNLKIVAPVREWAMSREEEIEYAK
SHGIPIPINLDSPYSIDQNLWGRSNECGILEDPWAEAPKDAYDLTIDPVDAPEDPEVIQITFKKGKPVSIDGKEMPLDEL
ILQLNTIAGKHGVGRIDHVENRLVGIKSREIYEAPAATTLIAAHQELEALTLPREVAEFKPTIEQKLAQTVYYGLWYSPL
TEALQSFIEKTQEHVSGTVKVKLYKGHAQVIGRESENSLYDFDLATYNKADAFDHDAALGFIKLWGLPTQVHSAVNGPQG
KKSIEKVDLEVKEAVKP
>Mature_416_residues
TKEKIVLAYSGGLDTSVAVKWLQDKYNYDVIAVALDVGEGKDLDFVKKKALDVGAIKSYVIDAKNLFAEEYVLPALQANL
LYEGKYPLISALSRPLISKILVDIAEEEGAVAVAHGCTGKGNDQVRFDVSFTALNPNLKIVAPVREWAMSREEEIEYAKS
HGIPIPINLDSPYSIDQNLWGRSNECGILEDPWAEAPKDAYDLTIDPVDAPEDPEVIQITFKKGKPVSIDGKEMPLDELI
LQLNTIAGKHGVGRIDHVENRLVGIKSREIYEAPAATTLIAAHQELEALTLPREVAEFKPTIEQKLAQTVYYGLWYSPLT
EALQSFIEKTQEHVSGTVKVKLYKGHAQVIGRESENSLYDFDLATYNKADAFDHDAALGFIKLWGLPTQVHSAVNGPQGK
KSIEKVDLEVKEAVKP

Specific function: Arginine biosynthesis; seventh step. [C]

COG id: COG0137

COG function: function code E; Argininosuccinate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the argininosuccinate synthase family. Type 1 subfamily

Homologues:

Organism=Homo sapiens, GI53759107, Length=399, Percent_Identity=45.8646616541353, Blast_Score=330, Evalue=1e-90,
Organism=Homo sapiens, GI16950633, Length=399, Percent_Identity=45.8646616541353, Blast_Score=330, Evalue=1e-90,
Organism=Escherichia coli, GI1789563, Length=408, Percent_Identity=27.6960784313726, Blast_Score=134, Evalue=8e-33,
Organism=Saccharomyces cerevisiae, GI6324514, Length=404, Percent_Identity=40.3465346534653, Blast_Score=299, Evalue=6e-82,
Organism=Drosophila melanogaster, GI21358151, Length=412, Percent_Identity=42.9611650485437, Blast_Score=311, Evalue=5e-85,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): ASSY_OCEIH (Q8ELT8)

Other databases:

- EMBL:   BA000028
- RefSeq:   NP_694051.1
- ProteinModelPortal:   Q8ELT8
- SMR:   Q8ELT8
- GeneID:   1018102
- GenomeReviews:   BA000028_GR
- KEGG:   oih:OB3129
- NMPDR:   fig|221109.1.peg.3132
- HOGENOM:   HBG335267
- OMA:   VAVDVGQ
- ProtClustDB:   PRK00509
- BioCyc:   OIHE221109:OB3129-MONOMER
- BRENDA:   6.3.4.5
- GO:   GO:0005737
- HAMAP:   MF_00005
- InterPro:   IPR001518
- InterPro:   IPR018223
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11587
- TIGRFAMs:   TIGR00032

Pfam domain/function: PF00764 Arginosuc_synth

EC number: =6.3.4.5

Molecular weight: Translated: 46069; Mature: 45938

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: PS00564 ARGININOSUCCIN_SYN_1; PS00565 ARGININOSUCCIN_SYN_2

Important sites: BINDING 87-87 BINDING 117-117 BINDING 119-119 BINDING 123-123 BINDING 123-123 BINDING 124-124 BINDING 127-127 BINDING 175-175 BINDING 184-184 BINDING 260-260 BINDING 272-272

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKEKIVLAYSGGLDTSVAVKWLQDKYNYDVIAVALDVGEGKDLDFVKKKALDVGAIKSY
CCCCEEEEEECCCCCHHHHHHHHHHCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHH
VIDAKNLFAEEYVLPALQANLLYEGKYPLISALSRPLISKILVDIAEEEGAVAVAHGCTG
HHHHHHHHHHHHHHHHHHHCEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCC
KGNDQVRFDVSFTALNPNLKIVAPVREWAMSREEEIEYAKSHGIPIPINLDSPYSIDQNL
CCCCEEEEEEEEEEECCCCEEEECHHHHHCCCHHHHHHHHHCCCEEEEECCCCCCCCHHH
WGRSNECGILEDPWAEAPKDAYDLTIDPVDAPEDPEVIQITFKKGKPVSIDGKEMPLDEL
CCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCEEEEEECCCCEECCCCCCCCHHHH
ILQLNTIAGKHGVGRIDHVENRLVGIKSREIYEAPAATTLIAAHQELEALTLPREVAEFK
HHHHHHHCCCCCCCCHHHHHHHEECCCCCHHHCCCCHHHHHHHHHHHHHHHCCHHHHHHC
PTIEQKLAQTVYYGLWYSPLTEALQSFIEKTQEHVSGTVKVKLYKGHAQVIGRESENSLY
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCEECCCCCCCEE
DFDLATYNKADAFDHDAALGFIKLWGLPTQVHSAVNGPQGKKSIEKVDLEVKEAVKP
EEEECCCCCCCCCCCHHHHHHHHCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TKEKIVLAYSGGLDTSVAVKWLQDKYNYDVIAVALDVGEGKDLDFVKKKALDVGAIKSY
CCCEEEEEECCCCCHHHHHHHHHHCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHH
VIDAKNLFAEEYVLPALQANLLYEGKYPLISALSRPLISKILVDIAEEEGAVAVAHGCTG
HHHHHHHHHHHHHHHHHHHCEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCC
KGNDQVRFDVSFTALNPNLKIVAPVREWAMSREEEIEYAKSHGIPIPINLDSPYSIDQNL
CCCCEEEEEEEEEEECCCCEEEECHHHHHCCCHHHHHHHHHCCCEEEEECCCCCCCCHHH
WGRSNECGILEDPWAEAPKDAYDLTIDPVDAPEDPEVIQITFKKGKPVSIDGKEMPLDEL
CCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCEEEEEECCCCEECCCCCCCCHHHH
ILQLNTIAGKHGVGRIDHVENRLVGIKSREIYEAPAATTLIAAHQELEALTLPREVAEFK
HHHHHHHCCCCCCCCHHHHHHHEECCCCCHHHCCCCHHHHHHHHHHHHHHHCCHHHHHHC
PTIEQKLAQTVYYGLWYSPLTEALQSFIEKTQEHVSGTVKVKLYKGHAQVIGRESENSLY
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCEECCCCCCCEE
DFDLATYNKADAFDHDAALGFIKLWGLPTQVHSAVNGPQGKKSIEKVDLEVKEAVKP
EEEECCCCCCCCCCCHHHHHHHHCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12235376