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The map label for this gene is argH

Identifier: 23100583

GI number: 23100583

Start: 3261124

End: 3262503

Strand: Reverse

Name: argH

Synonym: OB3128

Alternate gene names: 23100583

Gene position: 3262503-3261124 (Counterclockwise)

Preceding gene: 23100584

Following gene: 23100582

Centisome position: 89.86

GC content: 39.93

Gene sequence:

>1380_bases
ATGAAACTATGGGGCGGAAGATTTACAAAACCTACCAACGAATTGGTAGATGAATATGCATCATCCATTCAATTTGATAA
AAAACTAGCGGCTTATGACATTGAAGGTAGCCTAGCTCATGTTGCAATGTTGAAAAAATGTGAGATTATACCGGCTGAAG
ATGCCGATAAAATTGCAGAAGGTCTGAAAATAGTTTTATCTAAAATTGAAGCAGGAGAAGCAGAACTATCTAATGAACAT
GAAGATATTCATATGAACGTTGAAAAGCTATTAATTGATGAAGTGGGTCCAGTTGGTGGTAGGCTTCATACTGGCCGTAG
CAGAAACGACCAGGTAGCTCTAGATATGCGCCTGTATTTAAGGGATGTGATTGCCGAACTGTCTGATCTACTTAAGGCAG
TTCAACAATCATTAATTACCCAGGCGAAAGCAAACATGGATACCGTAATGCCAGGATATACTCACTTGCAACGCGCGCAA
CCAGTACTTTTTGCTCATCACATGATGGCTTATGTCTTTATGTTTCAGCGTGACGTTGAACGTTTTCAAGATAGTCTAAA
GCGTGTCAATAAATCCCCTTTAGGTGCTGGGGCTTTAGCAGGTACTACATTTCCAATTGATCGTCACTTCGTTGCGGAGC
AGCTAGGGTTTGATGGGATATGTGAGAATAGTCTGGATGCCGTAAGCGATCGGGATTTTGTTGTAGAGTTCTTATCCAAC
TCTGCGCTTGTTTCCACTCATTTATCCAGGCTTTGTGAGGAACTTGTTCAGTGGTCAAGTGCTGAATTTAACTTTGTGGA
GTTAGATGATTCTTTTACAACAGGAAGTAGCATGATGCCACAGAAGAAGAATCCGGATGTTGCGGAATTAGTCAGAGGAA
AGACAGGAAGAGTGTATGGACATTTAATGGGAATGTTAACCACGTTGAAAGGTCTTCCGCTTGCGTATAACAAAGATATG
CAGGAAGACAAAGAAGGTATGTTCGATGCGCATGAAACATTAAAAGGAGCCTTGCAATTATTTGCGCCAATGATGGAATC
GATGAAAGTAAATAAAGAAAGCATGTACAAGGCTGTATCTAATGATTATTCCAATGCAACTGATCTTGCGGATTATCTTG
TTAATAAGGGAATGACATTCCGAGAGTCGCACGCAGTCGTAGGAGAAGCTGTTTTACATTGTATAGAGCAAAATAAATAT
TTGCTAGATCTCACATTAAAAGAGTTTAAGCAGTATTCAGAAGTTATTGATGAAGATATCTTTGATGTTTTAAAGCCAGA
AGCAGTTGTCAATGCTCGTTCGGTAGAAGGTGGTACTGCAAAAGAAAGTGTGCTTCAGCAAATAGCAAAGGCGGAGAAAT
TATTGGAATCTGCTAAGTAG

Upstream 100 bases:

>100_bases
TTTGGGGACTACCTACGCAGGTTCACTCCGCTGTAAATGGACCACAAGGTAAAAAATCAATTGAAAAAGTAGACTTGGAA
GTAAAGGAAGCTGTGAAACC

Downstream 100 bases:

>100_bases
AATAGGGTATAGTAGAGAATGGTTACTCTGTTTGAAAAGGCGGTATGGCATATGAAATTAATTGCATCTGATTTAGATGG
TACACTTTTGCAAGAAGATG

Product: argininosuccinate lyase

Products: NA

Alternate protein names: ASAL; Arginosuccinase

Number of amino acids: Translated: 459; Mature: 459

Protein sequence:

>459_residues
MKLWGGRFTKPTNELVDEYASSIQFDKKLAAYDIEGSLAHVAMLKKCEIIPAEDADKIAEGLKIVLSKIEAGEAELSNEH
EDIHMNVEKLLIDEVGPVGGRLHTGRSRNDQVALDMRLYLRDVIAELSDLLKAVQQSLITQAKANMDTVMPGYTHLQRAQ
PVLFAHHMMAYVFMFQRDVERFQDSLKRVNKSPLGAGALAGTTFPIDRHFVAEQLGFDGICENSLDAVSDRDFVVEFLSN
SALVSTHLSRLCEELVQWSSAEFNFVELDDSFTTGSSMMPQKKNPDVAELVRGKTGRVYGHLMGMLTTLKGLPLAYNKDM
QEDKEGMFDAHETLKGALQLFAPMMESMKVNKESMYKAVSNDYSNATDLADYLVNKGMTFRESHAVVGEAVLHCIEQNKY
LLDLTLKEFKQYSEVIDEDIFDVLKPEAVVNARSVEGGTAKESVLQQIAKAEKLLESAK

Sequences:

>Translated_459_residues
MKLWGGRFTKPTNELVDEYASSIQFDKKLAAYDIEGSLAHVAMLKKCEIIPAEDADKIAEGLKIVLSKIEAGEAELSNEH
EDIHMNVEKLLIDEVGPVGGRLHTGRSRNDQVALDMRLYLRDVIAELSDLLKAVQQSLITQAKANMDTVMPGYTHLQRAQ
PVLFAHHMMAYVFMFQRDVERFQDSLKRVNKSPLGAGALAGTTFPIDRHFVAEQLGFDGICENSLDAVSDRDFVVEFLSN
SALVSTHLSRLCEELVQWSSAEFNFVELDDSFTTGSSMMPQKKNPDVAELVRGKTGRVYGHLMGMLTTLKGLPLAYNKDM
QEDKEGMFDAHETLKGALQLFAPMMESMKVNKESMYKAVSNDYSNATDLADYLVNKGMTFRESHAVVGEAVLHCIEQNKY
LLDLTLKEFKQYSEVIDEDIFDVLKPEAVVNARSVEGGTAKESVLQQIAKAEKLLESAK
>Mature_459_residues
MKLWGGRFTKPTNELVDEYASSIQFDKKLAAYDIEGSLAHVAMLKKCEIIPAEDADKIAEGLKIVLSKIEAGEAELSNEH
EDIHMNVEKLLIDEVGPVGGRLHTGRSRNDQVALDMRLYLRDVIAELSDLLKAVQQSLITQAKANMDTVMPGYTHLQRAQ
PVLFAHHMMAYVFMFQRDVERFQDSLKRVNKSPLGAGALAGTTFPIDRHFVAEQLGFDGICENSLDAVSDRDFVVEFLSN
SALVSTHLSRLCEELVQWSSAEFNFVELDDSFTTGSSMMPQKKNPDVAELVRGKTGRVYGHLMGMLTTLKGLPLAYNKDM
QEDKEGMFDAHETLKGALQLFAPMMESMKVNKESMYKAVSNDYSNATDLADYLVNKGMTFRESHAVVGEAVLHCIEQNKY
LLDLTLKEFKQYSEVIDEDIFDVLKPEAVVNARSVEGGTAKESVLQQIAKAEKLLESAK

Specific function: Arginine biosynthesis; eighth (last) step. [C]

COG id: COG0165

COG function: function code E; Argininosuccinate lyase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Argininosuccinate lyase subfamily

Homologues:

Organism=Homo sapiens, GI31541964, Length=456, Percent_Identity=42.1052631578947, Blast_Score=387, Evalue=1e-107,
Organism=Homo sapiens, GI68303542, Length=456, Percent_Identity=42.1052631578947, Blast_Score=387, Evalue=1e-107,
Organism=Homo sapiens, GI68303549, Length=456, Percent_Identity=39.9122807017544, Blast_Score=353, Evalue=2e-97,
Organism=Homo sapiens, GI68303547, Length=456, Percent_Identity=39.2543859649123, Blast_Score=348, Evalue=8e-96,
Organism=Escherichia coli, GI1790398, Length=456, Percent_Identity=50, Blast_Score=459, Evalue=1e-130,
Organism=Saccharomyces cerevisiae, GI6321806, Length=450, Percent_Identity=44.4444444444444, Blast_Score=386, Evalue=1e-108,
Organism=Drosophila melanogaster, GI221473854, Length=447, Percent_Identity=40.4921700223714, Blast_Score=360, Evalue=1e-99,
Organism=Drosophila melanogaster, GI78706858, Length=447, Percent_Identity=40.4921700223714, Blast_Score=360, Evalue=1e-99,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ARLY_OCEIH (Q8ELT9)

Other databases:

- EMBL:   BA000028
- RefSeq:   NP_694050.1
- ProteinModelPortal:   Q8ELT9
- SMR:   Q8ELT9
- GeneID:   1016602
- GenomeReviews:   BA000028_GR
- KEGG:   oih:OB3128
- NMPDR:   fig|221109.1.peg.3131
- HOGENOM:   HBG539632
- OMA:   EDIHTVI
- ProtClustDB:   CLSK2519633
- BioCyc:   OIHE221109:OB3128-MONOMER
- BRENDA:   4.3.2.1
- GO:   GO:0005737
- HAMAP:   MF_00006
- InterPro:   IPR009049
- InterPro:   IPR003031
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- PANTHER:   PTHR11444:SF3
- PRINTS:   PR00145
- PRINTS:   PR00149
- TIGRFAMs:   TIGR00838

Pfam domain/function: PF00206 Lyase_1; SSF48557 L-Aspartase-like

EC number: =4.3.2.1

Molecular weight: Translated: 51231; Mature: 51231

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure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HHHHCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12235376