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The map label for this gene is capL [H]

Identifier: 23100386

GI number: 23100386

Start: 3031191

End: 3032504

Strand: Direct

Name: capL [H]

Synonym: OB2931

Alternate gene names: 23100386

Gene position: 3031191-3032504 (Clockwise)

Preceding gene: 23100371

Following gene: 23100390

Centisome position: 83.49

GC content: 34.4

Gene sequence:

>1314_bases
ATGAGTTTATTCGAGAAAATTCAAAATAAAACAGAGAAAATTGCGGTAATTGGTTTAGGATATGTAGGGTTACCACTTGC
TGTAGAACTCGCAAAAAAATACGATGTTATTGGTCTTGATTTAAACAAAGAAAAGCTAGCACAATATTCAAATGGTATTG
ACGTGACCGCTGAAGTGGGTAACGAGGCTTTGAAGCAAACGACACTAGAATTTACGAGTGACGAGACTCGATTAAAAGAA
TGTAAATTTCACATTGTAGCTGTACCTACACCTATAAATACAGACAAAACTCCAAATCTCAAGCCAGTGATTGGAGCTAG
TGAATCGGTAGGCAGAAATATATCAAAAGGATCTATTGTTGTGTATGAATCAACTGTATATCCAGGTACAACGGAAGAAA
TATGTATTCCTATATTAGAAGAAAAATCCGGACTGAAATTTGGACAAGACTTTAAAGTAGGTTATTCACCTGAGAGAATC
AATCCTGGTGACAAAGTAAATACATTAGTTAGTATAACTAAGGTTGTCTCTGGTTCTGACGAAGATGCACTGAAAGAGAT
ATCTTCCTTATATGGATCGATCATTGAAGCCGGTGTGTTTGAAGCCGAATCAATTAAAGTAGCTGAAGCTGCAAAAGTAA
TAGAAAACTCTCAACGTGATATTAATATCGCCTTTATGAATGAATTATCCATGGTCTTTAATAAAATGGATATAGATACA
AAGGCAGTATTAGAAGCAGCGGGTACGAAGTGGAATTTCCTGAATTTCACACCTGGATTAGTTGGTGGCCATTGTATAGG
TGTCGATCCTTACTACTTCACATATAAAGCGGAACAATTAGGATATCATTCCCAAATCATTCTATCCGGCAGAAAAATTA
ATGATGATATGGGTAAATATATCGCAGGCAACATTATCAAAAAAATGATTAAATCTAAAATCGAAATTGATGGTGCAAGA
GTAGCTATTTTAGGTCTAACATTTAAAGAAAATGTTGGAGATGTAAGAAATACGAAAGTGAATGACATTGTAACCGAATT
AGAGGAATACGGTGTAAATGTCATTGTTCATGACCCAGTTGCAGAAGGTCAAGAAGTTAAAAAAGTGTTTGGAATAGAAC
TAGCTGAAGAAGATGATTTACAGGACCTTGATGCTGTTGTTTTAGCTGTACCGCATCAACACTATAAGGAATTAAATGTA
AATAAAATCAATACGATGTATAGACAAGACAATAAAAAAGTATTTATTGATATTAAAGGGTCACTTGATAAGAATGATTT
TTATCAAGCTGGCTATAGTTATTGGAGTCTGTAA

Upstream 100 bases:

>100_bases
TATCGAGTTCATATATAACTATACAAGAATACTATATAATTGTATAATATTGAGCAAAGATATTTCACTTTAATCACTAC
AAAAAGGTAGGCATATGATA

Downstream 100 bases:

>100_bases
TAGTAAGCTCGTATAGAAACGTAGTAATCTTCGCCTATCTTTTTGAAGAAATTCGAGAATGGGCGGGCTTCATTATATAG
TCCTCAATCAAACGTTTGAT

Product: VI polysaccharide biosynthesis

Products: UDP-N-acetyl-D-mannosaminuronic-acid; NADH; Proton [C]

Alternate protein names: NA

Number of amino acids: Translated: 437; Mature: 436

Protein sequence:

>437_residues
MSLFEKIQNKTEKIAVIGLGYVGLPLAVELAKKYDVIGLDLNKEKLAQYSNGIDVTAEVGNEALKQTTLEFTSDETRLKE
CKFHIVAVPTPINTDKTPNLKPVIGASESVGRNISKGSIVVYESTVYPGTTEEICIPILEEKSGLKFGQDFKVGYSPERI
NPGDKVNTLVSITKVVSGSDEDALKEISSLYGSIIEAGVFEAESIKVAEAAKVIENSQRDINIAFMNELSMVFNKMDIDT
KAVLEAAGTKWNFLNFTPGLVGGHCIGVDPYYFTYKAEQLGYHSQIILSGRKINDDMGKYIAGNIIKKMIKSKIEIDGAR
VAILGLTFKENVGDVRNTKVNDIVTELEEYGVNVIVHDPVAEGQEVKKVFGIELAEEDDLQDLDAVVLAVPHQHYKELNV
NKINTMYRQDNKKVFIDIKGSLDKNDFYQAGYSYWSL

Sequences:

>Translated_437_residues
MSLFEKIQNKTEKIAVIGLGYVGLPLAVELAKKYDVIGLDLNKEKLAQYSNGIDVTAEVGNEALKQTTLEFTSDETRLKE
CKFHIVAVPTPINTDKTPNLKPVIGASESVGRNISKGSIVVYESTVYPGTTEEICIPILEEKSGLKFGQDFKVGYSPERI
NPGDKVNTLVSITKVVSGSDEDALKEISSLYGSIIEAGVFEAESIKVAEAAKVIENSQRDINIAFMNELSMVFNKMDIDT
KAVLEAAGTKWNFLNFTPGLVGGHCIGVDPYYFTYKAEQLGYHSQIILSGRKINDDMGKYIAGNIIKKMIKSKIEIDGAR
VAILGLTFKENVGDVRNTKVNDIVTELEEYGVNVIVHDPVAEGQEVKKVFGIELAEEDDLQDLDAVVLAVPHQHYKELNV
NKINTMYRQDNKKVFIDIKGSLDKNDFYQAGYSYWSL
>Mature_436_residues
SLFEKIQNKTEKIAVIGLGYVGLPLAVELAKKYDVIGLDLNKEKLAQYSNGIDVTAEVGNEALKQTTLEFTSDETRLKEC
KFHIVAVPTPINTDKTPNLKPVIGASESVGRNISKGSIVVYESTVYPGTTEEICIPILEEKSGLKFGQDFKVGYSPERIN
PGDKVNTLVSITKVVSGSDEDALKEISSLYGSIIEAGVFEAESIKVAEAAKVIENSQRDINIAFMNELSMVFNKMDIDTK
AVLEAAGTKWNFLNFTPGLVGGHCIGVDPYYFTYKAEQLGYHSQIILSGRKINDDMGKYIAGNIIKKMIKSKIEIDGARV
AILGLTFKENVGDVRNTKVNDIVTELEEYGVNVIVHDPVAEGQEVKKVFGIELAEEDDLQDLDAVVLAVPHQHYKELNVN
KINTMYRQDNKKVFIDIKGSLDKNDFYQAGYSYWSL

Specific function: Required for the biosynthesis of type 1 capsular polysaccharide [H]

COG id: COG0677

COG function: function code M; UDP-N-acetyl-D-mannosaminuronate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the UDP-glucose/GDP-mannose dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI4507813, Length=436, Percent_Identity=22.4770642201835, Blast_Score=76, Evalue=7e-14,
Organism=Escherichia coli, GI48994968, Length=424, Percent_Identity=30.8962264150943, Blast_Score=176, Evalue=3e-45,
Organism=Caenorhabditis elegans, GI17560350, Length=476, Percent_Identity=23.109243697479, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI17136908, Length=466, Percent_Identity=26.8240343347639, Blast_Score=101, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008927
- InterPro:   IPR016040
- InterPro:   IPR017476
- InterPro:   IPR014027
- InterPro:   IPR014026
- InterPro:   IPR014028
- InterPro:   IPR001732 [H]

Pfam domain/function: PF00984 UDPG_MGDP_dh; PF03720 UDPG_MGDP_dh_C; PF03721 UDPG_MGDP_dh_N [H]

EC number: 1.1.1.- [C]

Molecular weight: Translated: 48328; Mature: 48196

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLFEKIQNKTEKIAVIGLGYVGLPLAVELAKKYDVIGLDLNKEKLAQYSNGIDVTAEVG
CCHHHHHCCCCCEEEEEEECHHCHHHHHHHHHHCCEEEECCCHHHHHHHCCCCEEEHHHH
NEALKQTTLEFTSDETRLKECKFHIVAVPTPINTDKTPNLKPVIGASESVGRNISKGSIV
HHHHHHHHHHCCCCHHHHHHCEEEEEEECCCCCCCCCCCCCEECCCCHHHCCCCCCCEEE
VYESTVYPGTTEEICIPILEEKSGLKFGQDFKVGYSPERINPGDKVNTLVSITKVVSGSD
EEECCCCCCCCHHHEEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCC
EDALKEISSLYGSIIEAGVFEAESIKVAEAAKVIENSQRDINIAFMNELSMVFNKMDIDT
HHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHCCCCH
KAVLEAAGTKWNFLNFTPGLVGGHCIGVDPYYFTYKAEQLGYHSQIILSGRKINDDMGKY
HHHHHHCCCCEEEEECCCCCCCCEEEECCCEEEEEEHHHCCCCEEEEEECCCCCHHHHHH
IAGNIIKKMIKSKIEIDGARVAILGLTFKENVGDVRNTKVNDIVTELEEYGVNVIVHDPV
HHHHHHHHHHHHHHCCCCCEEEEEEEEEHHCCCCCCCCHHHHHHHHHHHCCCEEEEECCC
AEGQEVKKVFGIELAEEDDLQDLDAVVLAVPHQHYKELNVNKINTMYRQDNKKVFIDIKG
CCCHHHHHHHCCEECCCCCHHHHHHEEEECCHHHHHHCCCHHHHHHHCCCCCEEEEEEEC
SLDKNDFYQAGYSYWSL
CCCCCHHHHHCCHHCCC
>Mature Secondary Structure 
SLFEKIQNKTEKIAVIGLGYVGLPLAVELAKKYDVIGLDLNKEKLAQYSNGIDVTAEVG
CHHHHHCCCCCEEEEEEECHHCHHHHHHHHHHCCEEEECCCHHHHHHHCCCCEEEHHHH
NEALKQTTLEFTSDETRLKECKFHIVAVPTPINTDKTPNLKPVIGASESVGRNISKGSIV
HHHHHHHHHHCCCCHHHHHHCEEEEEEECCCCCCCCCCCCCEECCCCHHHCCCCCCCEEE
VYESTVYPGTTEEICIPILEEKSGLKFGQDFKVGYSPERINPGDKVNTLVSITKVVSGSD
EEECCCCCCCCHHHEEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCC
EDALKEISSLYGSIIEAGVFEAESIKVAEAAKVIENSQRDINIAFMNELSMVFNKMDIDT
HHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHCCCCH
KAVLEAAGTKWNFLNFTPGLVGGHCIGVDPYYFTYKAEQLGYHSQIILSGRKINDDMGKY
HHHHHHCCCCEEEEECCCCCCCCEEEECCCEEEEEEHHHCCCCEEEEEECCCCCHHHHHH
IAGNIIKKMIKSKIEIDGARVAILGLTFKENVGDVRNTKVNDIVTELEEYGVNVIVHDPV
HHHHHHHHHHHHHHCCCCCEEEEEEEEEHHCCCCCCCCHHHHHHHHHHHCCCEEEEECCC
AEGQEVKKVFGIELAEEDDLQDLDAVVLAVPHQHYKELNVNKINTMYRQDNKKVFIDIKG
CCCHHHHHHHCCEECCCCCHHHHHHEEEECCHHHHHHCCCHHHHHHHCCCCCEEEEEEEC
SLDKNDFYQAGYSYWSL
CCCCCHHHHHCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: UDP-N-acetyl-D-mannosamine; NAD [C]

Specific reaction: UDP-N-acetyl-D-mannosamine + NAD = UDP-N-acetyl-D-mannosaminuronic-acid + NADH + Proton [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7961465 [H]