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The map label for this gene is cphA [H]

Identifier: 23100371

GI number: 23100371

Start: 3010348

End: 3012012

Strand: Direct

Name: cphA [H]

Synonym: OB2916

Alternate gene names: 23100371

Gene position: 3010348-3012012 (Clockwise)

Preceding gene: 23100368

Following gene: 23100386

Centisome position: 82.92

GC content: 31.41

Gene sequence:

>1665_bases
ATGGTAGACAATCGCTTTTTAGAAAGTTTAACTCATGATATACCTGAAAAAGCAAATAATTATATGCTTAGCACATATAG
TATAATTTTAGAAGCCTGGCGTAGAGGATTAGATATTAACATACGTATATTAAAAGAAAAATCTGGATCGATAGAACCTT
ACTACTCAATTAGTAATGGTGATAAAGTTCATCATTTTAGTGCTACAAGAGGTGATTTGGTTTCCAAGGAAGCTAAGGAG
CTTACTAAAAATAAAGTAACGACCAAACAAATTCTAAATAAATATAAGGTTCCAACTCCTGAAGGCAAAGAATTTGAGGA
AGAAGCTACTACAGAAGAAATTATTAGTTATGCTACAGCAATCGATTATCCTTTAGTAGTAAAGCCTGTATCTGGTACAG
GTGGAAAAGGTGTTATTGCAGGAATTCAAAACGAAGAAGAGTTAGTAGAAGCGTTAGAATACGTGCGAGAAAAGTTAAAT
TCACCTAGAATTATTCTTGAAAAGTATTTTGAAGGTGAAGATTATCGGATTTATGTGATAGATGGACAAGTCATTGCAGC
ATTAAAGAGGATAAAAGCTAATATTATAGGAAACGGAAATGACACTATAAAGAACTTAATAGATAAAAAGAATAAGTATC
GTTCTCAGCTCCCATCATTGACAAATCGGCCAATTAAAGTAGATGATGAAACAAAAACGCTTATCCGAAGAGCAGGATAT
ACAATGGATTCTGTTTTACCTGATGGTGAATTACTGTATATTAAAACGAAGAATAATGTATCGGCCGGCGGGGACTCTAT
TGATATAACAGAACAACTATCTGAAAGTATTAAACAAATCGCAATTGATGCAACTAATTGTTTTGGATCACTTCCCCATT
GTGGCTTAGATTTGATGGTTGATGAAGCTAATGACAAGGCAGTTATTATAGAAATCAATTCACGAGCCCATATTACACAG
CATCTATTTCCAATGGAAGGCCAAGCTAGGGATATCCCTGGAAGTTTAATTGATTTTTACTTTCCTGAGACCAAAAATTA
TAATCGCTTAGATTCTTTTAAAATGTTTATTGATTTCGAATATATTTATGATTCCTGTATAAGTAGAGAAGCTGCTGAAA
TACGCATACCAAAGAAACCAGAAGGTCCAATCTTATTAACACGTTATTTAATAAACGGCGTAAAATTAACAGATAAATTT
GCAGCTCGAGTTAAACGATTAGCTTATAATACTCAAGTTAGTGGTTATATAAAACCTTTAAATAATGGTGATATCTCTAT
TATCGTTGGAGGCAATGAAAATAAGATTGAACAATTTAAAAAGAGCTTAAATAAATATCTACCTAAATTCAGTAAAAAAT
ATGAAATTACAGCTAAGAAACGTACTACCCCTATACCTCACGGATTTCATATACATGATAATAAAGCTCAGGATTCTAAT
AATGCAGTATCGGCTTCTACAAATGAATATATGAAGAAATATAGTAATCTTCAATCTGATTATCAACAACTTATAAGGAA
AGTTGCTGAATATGAAAAAAGAGAGAGATTATTAGAAATAACTCAAAAACAAAATAAGCAATTGAAGAAAAGGCTTAAAT
TAATGGAAAATAGTACTTCTTGGAAAATCACTAAACCAATTAGGAAATTAACACGAAAAAAATAA

Upstream 100 bases:

>100_bases
TACATTATAAATAAAAAGCTATAACTACAAAATGGAATGAATAAGCAACTATGGTATAATATTATCGGGGAATAATACCT
GTAAGTAAAGGGGAATAGAT

Downstream 100 bases:

>100_bases
TTAGCCTCGCTTACCTAAAAAAGTCCGAACCTCCCACTTAGGGTGATTCAGGACTTTTTTAATTATCTTACTTCTAACTC
TGGCATCCATTTTGTTAATC

Product: hypothetical protein

Products: NA

Alternate protein names: Cyanophycin synthase [H]

Number of amino acids: Translated: 554; Mature: 554

Protein sequence:

>554_residues
MVDNRFLESLTHDIPEKANNYMLSTYSIILEAWRRGLDINIRILKEKSGSIEPYYSISNGDKVHHFSATRGDLVSKEAKE
LTKNKVTTKQILNKYKVPTPEGKEFEEEATTEEIISYATAIDYPLVVKPVSGTGGKGVIAGIQNEEELVEALEYVREKLN
SPRIILEKYFEGEDYRIYVIDGQVIAALKRIKANIIGNGNDTIKNLIDKKNKYRSQLPSLTNRPIKVDDETKTLIRRAGY
TMDSVLPDGELLYIKTKNNVSAGGDSIDITEQLSESIKQIAIDATNCFGSLPHCGLDLMVDEANDKAVIIEINSRAHITQ
HLFPMEGQARDIPGSLIDFYFPETKNYNRLDSFKMFIDFEYIYDSCISREAAEIRIPKKPEGPILLTRYLINGVKLTDKF
AARVKRLAYNTQVSGYIKPLNNGDISIIVGGNENKIEQFKKSLNKYLPKFSKKYEITAKKRTTPIPHGFHIHDNKAQDSN
NAVSASTNEYMKKYSNLQSDYQQLIRKVAEYEKRERLLEITQKQNKQLKKRLKLMENSTSWKITKPIRKLTRKK

Sequences:

>Translated_554_residues
MVDNRFLESLTHDIPEKANNYMLSTYSIILEAWRRGLDINIRILKEKSGSIEPYYSISNGDKVHHFSATRGDLVSKEAKE
LTKNKVTTKQILNKYKVPTPEGKEFEEEATTEEIISYATAIDYPLVVKPVSGTGGKGVIAGIQNEEELVEALEYVREKLN
SPRIILEKYFEGEDYRIYVIDGQVIAALKRIKANIIGNGNDTIKNLIDKKNKYRSQLPSLTNRPIKVDDETKTLIRRAGY
TMDSVLPDGELLYIKTKNNVSAGGDSIDITEQLSESIKQIAIDATNCFGSLPHCGLDLMVDEANDKAVIIEINSRAHITQ
HLFPMEGQARDIPGSLIDFYFPETKNYNRLDSFKMFIDFEYIYDSCISREAAEIRIPKKPEGPILLTRYLINGVKLTDKF
AARVKRLAYNTQVSGYIKPLNNGDISIIVGGNENKIEQFKKSLNKYLPKFSKKYEITAKKRTTPIPHGFHIHDNKAQDSN
NAVSASTNEYMKKYSNLQSDYQQLIRKVAEYEKRERLLEITQKQNKQLKKRLKLMENSTSWKITKPIRKLTRKK
>Mature_554_residues
MVDNRFLESLTHDIPEKANNYMLSTYSIILEAWRRGLDINIRILKEKSGSIEPYYSISNGDKVHHFSATRGDLVSKEAKE
LTKNKVTTKQILNKYKVPTPEGKEFEEEATTEEIISYATAIDYPLVVKPVSGTGGKGVIAGIQNEEELVEALEYVREKLN
SPRIILEKYFEGEDYRIYVIDGQVIAALKRIKANIIGNGNDTIKNLIDKKNKYRSQLPSLTNRPIKVDDETKTLIRRAGY
TMDSVLPDGELLYIKTKNNVSAGGDSIDITEQLSESIKQIAIDATNCFGSLPHCGLDLMVDEANDKAVIIEINSRAHITQ
HLFPMEGQARDIPGSLIDFYFPETKNYNRLDSFKMFIDFEYIYDSCISREAAEIRIPKKPEGPILLTRYLINGVKLTDKF
AARVKRLAYNTQVSGYIKPLNNGDISIIVGGNENKIEQFKKSLNKYLPKFSKKYEITAKKRTTPIPHGFHIHDNKAQDSN
NAVSASTNEYMKKYSNLQSDYQQLIRKVAEYEKRERLLEITQKQNKQLKKRLKLMENSTSWKITKPIRKLTRKK

Specific function: Catalyzes the ATP-dependent polymerization of arginine and aspartate to multi-L-arginyl-poly-L-aspartic acid (cyanophycin; a water-insoluble reserve polymer) [H]

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013651
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR011810
- InterPro:   IPR004101
- InterPro:   IPR013221 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M; PF08443 RimK [H]

EC number: =6.3.2.29; =6.3.2.30 [H]

Molecular weight: Translated: 63296; Mature: 63296

Theoretical pI: Translated: 9.61; Mature: 9.61

Prosite motif: PS50975 ATP_GRASP ; PS51160 ACYLPHOSPHATASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVDNRFLESLTHDIPEKANNYMLSTYSIILEAWRRGLDINIRILKEKSGSIEPYYSISNG
CCCHHHHHHHHHHHHHHHCCEEHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCEEEECCC
DKVHHFSATRGDLVSKEAKELTKNKVTTKQILNKYKVPTPEGKEFEEEATTEEIISYATA
CEEEEECCCCCHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHH
IDYPLVVKPVSGTGGKGVIAGIQNEEELVEALEYVREKLNSPRIILEKYFEGEDYRIYVI
CCCCEEEEECCCCCCCEEEECCCCHHHHHHHHHHHHHHHCCCEEEEHHHCCCCCEEEEEE
DGQVIAALKRIKANIIGNGNDTIKNLIDKKNKYRSQLPSLTNRPIKVDDETKTLIRRAGY
CCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCHHCCCCEEECCHHHHHHHHCCC
TMDSVLPDGELLYIKTKNNVSAGGDSIDITEQLSESIKQIAIDATNCFGSLPHCGLDLMV
CHHHCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEE
DEANDKAVIIEINSRAHITQHLFPMEGQARDIPGSLIDFYFPETKNYNRLDSFKMFIDFE
ECCCCCEEEEEECCCCHHHHHCCCCCCCCCCCCCHHHHHCCCCCCCCCCCCCEEEEEEHH
YIYDSCISREAAEIRIPKKPEGPILLTRYLINGVKLTDKFAARVKRLAYNTQVSGYIKPL
HHHHHHHCCCHHEEECCCCCCCCEEEEHHHHCCCCCHHHHHHHHHHHHHCCCCCEEEEEC
NNGDISIIVGGNENKIEQFKKSLNKYLPKFSKKYEITAKKRTTPIPHGFHIHDNKAQDSN
CCCCEEEEECCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCEEECCCCCCCCC
NAVSASTNEYMKKYSNLQSDYQQLIRKVAEYEKRERLLEITQKQNKQLKKRLKLMENSTS
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
WKITKPIRKLTRKK
EEEHHHHHHHHCCC
>Mature Secondary Structure
MVDNRFLESLTHDIPEKANNYMLSTYSIILEAWRRGLDINIRILKEKSGSIEPYYSISNG
CCCHHHHHHHHHHHHHHHCCEEHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCEEEECCC
DKVHHFSATRGDLVSKEAKELTKNKVTTKQILNKYKVPTPEGKEFEEEATTEEIISYATA
CEEEEECCCCCHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHH
IDYPLVVKPVSGTGGKGVIAGIQNEEELVEALEYVREKLNSPRIILEKYFEGEDYRIYVI
CCCCEEEEECCCCCCCEEEECCCCHHHHHHHHHHHHHHHCCCEEEEHHHCCCCCEEEEEE
DGQVIAALKRIKANIIGNGNDTIKNLIDKKNKYRSQLPSLTNRPIKVDDETKTLIRRAGY
CCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCHHCCCCEEECCHHHHHHHHCCC
TMDSVLPDGELLYIKTKNNVSAGGDSIDITEQLSESIKQIAIDATNCFGSLPHCGLDLMV
CHHHCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEE
DEANDKAVIIEINSRAHITQHLFPMEGQARDIPGSLIDFYFPETKNYNRLDSFKMFIDFE
ECCCCCEEEEEECCCCHHHHHCCCCCCCCCCCCCHHHHHCCCCCCCCCCCCCEEEEEEHH
YIYDSCISREAAEIRIPKKPEGPILLTRYLINGVKLTDKFAARVKRLAYNTQVSGYIKPL
HHHHHHHCCCHHEEECCCCCCCCEEEEHHHHCCCCCHHHHHHHHHHHHHCCCCCEEEEEC
NNGDISIIVGGNENKIEQFKKSLNKYLPKFSKKYEITAKKRTTPIPHGFHIHDNKAQDSN
CCCCEEEEECCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCEEECCCCCCCCC
NAVSASTNEYMKKYSNLQSDYQQLIRKVAEYEKRERLLEITQKQNKQLKKRLKLMENSTS
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
WKITKPIRKLTRKK
EEEHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9652408 [H]