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The map label for this gene is cphA [H]

Identifier: 23100374

GI number: 23100374

Start: 3014802

End: 3016421

Strand: Reverse

Name: cphA [H]

Synonym: OB2919

Alternate gene names: 23100374

Gene position: 3016421-3014802 (Counterclockwise)

Preceding gene: 23100375

Following gene: 23100373

Centisome position: 83.08

GC content: 36.48

Gene sequence:

>1620_bases
ATGAATGAGAATAATGCAGAATGGCTATCACACCTTTCTGAAGACATAGTTGCAGATGCACACGGAAATTTACTTGATTC
TTATGTAATAGCCCTTGAAGGTTGGAGAAGAGGGCTTAAATTAAGATGGCATGTTAAAGATTCGGAGAAATTTAGTGAAA
TGAGGACATGGTTTGTGGATAGACCGGGACAATTATTTTCATTAAGTTCTAAGCAAAACACACATTACTTCTTCCGTACT
CGAGGAGATAAAGTTTCTAATGATGCAGTAGATATTGGTAAAGATAAAGAAAGAACAAAAAAGATACTATCTGAGAATGG
GGTACGTGTTCCGGATGGAAAGCAATTTAAAAAAGAGATAACGAATCAAGAGATTATTGAGTATGCTGGACAGGTAGGTT
ATCCAATTGTAATCAAACCAACTGATGGTAGCTTTGGAAGAGGGGTAATTTCTAATATCACAAGCTCTAGTGAACTAGAG
TATTCATTAGACTACTTACGAAATGAATTAGGTTATCAAGATATTATCTTAGAGCAATTTATTCCAGGGAAAGATTACAG
ACTGTATGTTGTTGGTGATAAAGCTGTAGCTGCTATGCACCGTATGCCACCAAATATTATTGGAGATGGCGTAAATACTG
TACGTGCATTAATCGATATTAAGAACAACGAACGTAAACTGAATCCAAGATTGGTTAGTTGTTTAATAAAGGCTGATGAT
GAAAGTATTAATTATATTGGAAGACAAGGGTATACCTTAGAGTCAGTACCAGAAAATGGAGAACAGGTATACCTAAATTC
AAAAGGTAATATTTCTTTAGGCGGTGATCCGATTGATGTCTTTGATGAACTTCCTCAGCATGTAAAAGACTATGCTGTAA
AGGCGGTTCAAGCTGTACCAGGACTTGCTCATGGTGCCGTAGATATGATTTGTGATCCTGATACGAATGAGTCATACATT
ATTGAGCTAAATCCAACCTCGCAATTAGGAGGATTGCTTTATCCTATTCAAGGAAAGTCACGCGATATTCCAAATGCGAT
AATCGATTATTACTTTCCAGAAACTATATCAGACAAGAAGAAAACAAATACCTATTTTGATTTTCATGATGTTCTTGATC
CACTTCAAAATAGAGATGCGGTTGTTACAACAGTAACTCCTTGTCCGCAAGAGAAAATTTATATGAAAAAATATATTGTT
ACTGGGAATGTACAAGAGCTAGGTTATCACCGAGGTTTGAGAAAACAAGCGTTTGAGCGTTTTCTCCACGGTTATATTAT
GAAGATGGATAATGGAGACTTAGAAGTAGTTGTTGGAGGAGCAGACCCCGAAATGGTGGATGACTTCAAACATGCTTTCT
GGGAAGACGAAGAAAGAGCACAAGTAATGGAAGTTAGTGAAGAGAATTACGATGAACCTATAAAAGTCGGATTTGAAATA
AAGACAGATTTAAAAACACAACTGGAAGAATTAAAGATGTATAAGCAAGAGTTAGAAGTAACAGAAAGAGCACTAAAGAA
AGCAGAAGTAGAGCGTAGGAAGTATTATGAAAGCTTCTCTTGGAAAGCAACGACGCCAGTACGAGCAGTCGGCTCCATGT
TTAAGAAATTGAAGAAGTAA

Upstream 100 bases:

>100_bases
CGAACATGATTTAATAAATGGAAAGCAATTTGAAGAATATAAAAAAATAAAACAAGAAGCAAATAATGATAGTGTAGGAT
AGGTTTTAGGAGGATAACAA

Downstream 100 bases:

>100_bases
GGAAAAATAGGAGGAATAAAGAATGAAAGAGAGACCATTAGGAATTAGTCTTCCTCATTTAACAAATGAAATAACAAGAA
ATGCAAGAAAAACAAGACTT

Product: hypothetical protein

Products: NA

Alternate protein names: Cyanophycin synthase [H]

Number of amino acids: Translated: 539; Mature: 539

Protein sequence:

>539_residues
MNENNAEWLSHLSEDIVADAHGNLLDSYVIALEGWRRGLKLRWHVKDSEKFSEMRTWFVDRPGQLFSLSSKQNTHYFFRT
RGDKVSNDAVDIGKDKERTKKILSENGVRVPDGKQFKKEITNQEIIEYAGQVGYPIVIKPTDGSFGRGVISNITSSSELE
YSLDYLRNELGYQDIILEQFIPGKDYRLYVVGDKAVAAMHRMPPNIIGDGVNTVRALIDIKNNERKLNPRLVSCLIKADD
ESINYIGRQGYTLESVPENGEQVYLNSKGNISLGGDPIDVFDELPQHVKDYAVKAVQAVPGLAHGAVDMICDPDTNESYI
IELNPTSQLGGLLYPIQGKSRDIPNAIIDYYFPETISDKKKTNTYFDFHDVLDPLQNRDAVVTTVTPCPQEKIYMKKYIV
TGNVQELGYHRGLRKQAFERFLHGYIMKMDNGDLEVVVGGADPEMVDDFKHAFWEDEERAQVMEVSEENYDEPIKVGFEI
KTDLKTQLEELKMYKQELEVTERALKKAEVERRKYYESFSWKATTPVRAVGSMFKKLKK

Sequences:

>Translated_539_residues
MNENNAEWLSHLSEDIVADAHGNLLDSYVIALEGWRRGLKLRWHVKDSEKFSEMRTWFVDRPGQLFSLSSKQNTHYFFRT
RGDKVSNDAVDIGKDKERTKKILSENGVRVPDGKQFKKEITNQEIIEYAGQVGYPIVIKPTDGSFGRGVISNITSSSELE
YSLDYLRNELGYQDIILEQFIPGKDYRLYVVGDKAVAAMHRMPPNIIGDGVNTVRALIDIKNNERKLNPRLVSCLIKADD
ESINYIGRQGYTLESVPENGEQVYLNSKGNISLGGDPIDVFDELPQHVKDYAVKAVQAVPGLAHGAVDMICDPDTNESYI
IELNPTSQLGGLLYPIQGKSRDIPNAIIDYYFPETISDKKKTNTYFDFHDVLDPLQNRDAVVTTVTPCPQEKIYMKKYIV
TGNVQELGYHRGLRKQAFERFLHGYIMKMDNGDLEVVVGGADPEMVDDFKHAFWEDEERAQVMEVSEENYDEPIKVGFEI
KTDLKTQLEELKMYKQELEVTERALKKAEVERRKYYESFSWKATTPVRAVGSMFKKLKK
>Mature_539_residues
MNENNAEWLSHLSEDIVADAHGNLLDSYVIALEGWRRGLKLRWHVKDSEKFSEMRTWFVDRPGQLFSLSSKQNTHYFFRT
RGDKVSNDAVDIGKDKERTKKILSENGVRVPDGKQFKKEITNQEIIEYAGQVGYPIVIKPTDGSFGRGVISNITSSSELE
YSLDYLRNELGYQDIILEQFIPGKDYRLYVVGDKAVAAMHRMPPNIIGDGVNTVRALIDIKNNERKLNPRLVSCLIKADD
ESINYIGRQGYTLESVPENGEQVYLNSKGNISLGGDPIDVFDELPQHVKDYAVKAVQAVPGLAHGAVDMICDPDTNESYI
IELNPTSQLGGLLYPIQGKSRDIPNAIIDYYFPETISDKKKTNTYFDFHDVLDPLQNRDAVVTTVTPCPQEKIYMKKYIV
TGNVQELGYHRGLRKQAFERFLHGYIMKMDNGDLEVVVGGADPEMVDDFKHAFWEDEERAQVMEVSEENYDEPIKVGFEI
KTDLKTQLEELKMYKQELEVTERALKKAEVERRKYYESFSWKATTPVRAVGSMFKKLKK

Specific function: Catalyzes the ATP-dependent polymerization of arginine and aspartate to multi-L-arginyl-poly-L-aspartic acid (cyanophycin; a water-insoluble reserve polymer) [H]

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013651
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR011810
- InterPro:   IPR004101
- InterPro:   IPR013221 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M; PF08443 RimK [H]

EC number: =6.3.2.29; =6.3.2.30 [H]

Molecular weight: Translated: 61573; Mature: 61573

Theoretical pI: Translated: 5.26; Mature: 5.26

Prosite motif: PS50975 ATP_GRASP ; PS51160 ACYLPHOSPHATASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNENNAEWLSHLSEDIVADAHGNLLDSYVIALEGWRRGLKLRWHVKDSEKFSEMRTWFVD
CCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHCC
RPGQLFSLSSKQNTHYFFRTRGDKVSNDAVDIGKDKERTKKILSENGVRVPDGKQFKKEI
CCCCEEEECCCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHH
TNQEIIEYAGQVGYPIVIKPTDGSFGRGVISNITSSSELEYSLDYLRNELGYQDIILEQF
CHHHHHHHHHCCCCCEEEECCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHCHHHHHHHHC
IPGKDYRLYVVGDKAVAAMHRMPPNIIGDGVNTVRALIDIKNNERKLNPRLVSCLIKADD
CCCCCEEEEEECCHHHHHHHHCCCCHHCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHCCC
ESINYIGRQGYTLESVPENGEQVYLNSKGNISLGGDPIDVFDELPQHVKDYAVKAVQAVP
CCHHHHCCCCCEECCCCCCCCEEEEECCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHCC
GLAHGAVDMICDPDTNESYIIELNPTSQLGGLLYPIQGKSRDIPNAIIDYYFPETISDKK
CCCCCCEEEEECCCCCCCEEEEECCCHHHCCEEEECCCCCCCCCHHHHHHCCCCCCCCCC
KTNTYFDFHDVLDPLQNRDAVVTTVTPCPQEKIYMKKYIVTGNVQELGYHRGLRKQAFER
CCCCEEEHHHHHHHHCCCCEEEEEECCCCCHHHHHHHEEEECCHHHHHHHCCHHHHHHHH
FLHGYIMKMDNGDLEVVVGGADPEMVDDFKHAFWEDEERAQVMEVSEENYDEPIKVGFEI
HHHHEEEEECCCCEEEEECCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCEEEEEEE
KTDLKTQLEELKMYKQELEVTERALKKAEVERRKYYESFSWKATTPVRAVGSMFKKLKK
EHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MNENNAEWLSHLSEDIVADAHGNLLDSYVIALEGWRRGLKLRWHVKDSEKFSEMRTWFVD
CCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHCC
RPGQLFSLSSKQNTHYFFRTRGDKVSNDAVDIGKDKERTKKILSENGVRVPDGKQFKKEI
CCCCEEEECCCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHH
TNQEIIEYAGQVGYPIVIKPTDGSFGRGVISNITSSSELEYSLDYLRNELGYQDIILEQF
CHHHHHHHHHCCCCCEEEECCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHCHHHHHHHHC
IPGKDYRLYVVGDKAVAAMHRMPPNIIGDGVNTVRALIDIKNNERKLNPRLVSCLIKADD
CCCCCEEEEEECCHHHHHHHHCCCCHHCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHCCC
ESINYIGRQGYTLESVPENGEQVYLNSKGNISLGGDPIDVFDELPQHVKDYAVKAVQAVP
CCHHHHCCCCCEECCCCCCCCEEEEECCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHCC
GLAHGAVDMICDPDTNESYIIELNPTSQLGGLLYPIQGKSRDIPNAIIDYYFPETISDKK
CCCCCCEEEEECCCCCCCEEEEECCCHHHCCEEEECCCCCCCCCHHHHHHCCCCCCCCCC
KTNTYFDFHDVLDPLQNRDAVVTTVTPCPQEKIYMKKYIVTGNVQELGYHRGLRKQAFER
CCCCEEEHHHHHHHHCCCCEEEEEECCCCCHHHHHHHEEEECCHHHHHHHCCHHHHHHHH
FLHGYIMKMDNGDLEVVVGGADPEMVDDFKHAFWEDEERAQVMEVSEENYDEPIKVGFEI
HHHHEEEEECCCCEEEEECCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCEEEEEEE
KTDLKTQLEELKMYKQELEVTERALKKAEVERRKYYESFSWKATTPVRAVGSMFKKLKK
EHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA