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The map label for this gene is gshAB [H]

Identifier: 23100373

GI number: 23100373

Start: 3013127

End: 3014779

Strand: Reverse

Name: gshAB [H]

Synonym: OB2918

Alternate gene names: 23100373

Gene position: 3014779-3013127 (Counterclockwise)

Preceding gene: 23100374

Following gene: 23100372

Centisome position: 83.04

GC content: 35.57

Gene sequence:

>1653_bases
ATGAAAGAGAGACCATTAGGAATTAGTCTTCCTCATTTAACAAATGAAATAACAAGAAATGCAAGAAAAACAAGACTTGA
TGCTTTTGCAGTAGCATTGGAAGGCTGGAGAAGAGGACTAAAATTAAAGTGGTATACAAAAGACTCTGAGCATTTTCAAG
ATATGATTATATTCGGAGTGAATCCACCTGGAAGATTATTTTCACTTTCCTCTGAAGAACAAACCCATTACTTCTTTCGA
ACACGTGGAGACCTTGTTTCTAATGAAGCTGTTGATATAGGTTCTGAGAAAGATGATACGAAAATCTATATGGAAAAAGC
TGGTGTACCTATTCCATTAGGAAAAGGATTTGAGCCGGAAAGTACGAATGAAGAAATTATTGAATATAGTAAGACCCTAG
GGTTTCCTCTAGTCCTTAAACCAACCAATGCCAGTCTTGGGAACGGTGTAGTAACAAATATAAAAAATGAAGAGCAATTA
ATAAAAGCATTACACTATGTAAGAACGGAGTTAGATTATAAAGAAGTCGTTGTAGAACAATATGTAAGTGGTAAGGAATA
TCGTGTATATGTTGTTGGTGATGAAGTAGTTGCAGCTTATAATCGCGTACCCGCCAATATTATTGGGGATGGGGAACATA
CAATAGAAGAATTAATCAGTATGAAGAATAATGTACGAAAACAGAATGCTCGGCTCAACAGCTGTCTTATTCATATGGAT
GTAGAAATCTTAGAATTTATTGAAGAGGCAGGATATAGTCTACAGAGTGTTCCGGATAAAGGAAAGGTTATTTATCTACG
TGAGAAGACAAATGTATCTTCTGGTGGGGATCCAGTAGATGTAACTGACAGCCTTCCTGAAGAGTACAAAAAAATTGCAA
TTGATGCTTTGAAAGCTGTCCCAGACTTTCCACACGGGGGAGTAGACATTATTGTAAATGATCGCGGAGATTTAAAAGGT
GCGGCTGTGGTTATAGAATTAAATCCTACAGCACAAATTGGTGGTGCTCTATATCCAATAGAAGGAAAAGCAAGAGATAT
TCCTAAAGCAATCGTTGATTATTATTTCCCGGAAACAAAAGGACAAGTAGACACGAGAAAATCAAAAGTATATTTTGATT
TAATTACCTTGCTAGAACCTCTTGAAAATAGAGCTGCTTTAGAAGTGGAGGTAGCTCCGGCACCAATAGGAGAACTGTAT
GCGAAACAATATATTGTAAAAGGGAATGTACAAAGATATAACTTCCATAAATGGCTGAAGCAACAAGCCATACAACATAA
TTTACATGGTTACGTTAAAAGTATTGTATTCGATGAGATTGAAATAGTTGTCGCTAGTACCGATAAGAAAACTGTTGAAG
GTTTTAAAGATATCATACAAGGGTATCCACAAGGATCAAGTATTTCTAAAATCAAAGAGGAAGTATACGAAGATCCAATA
ACAGTTGGGTTCGAAATTTCTGAACAGTATAATACAAATAATGTACGATCTGTACAACATGCTTTACGTAAGATGGACAG
AGATTTGCAACGTATGAGGAAGTTAAAAAACCGAGCTGATAAAGACTTAAAACATATTTTAAGAAGTAGCTCTTGGAAGT
ATACGGAACCTGTAAGAAAGCTGGGAGGACTAATAAAGACAAAAAATAAATAA

Upstream 100 bases:

>100_bases
TATTATGAAAGCTTCTCTTGGAAAGCAACGACGCCAGTACGAGCAGTCGGCTCCATGTTTAAGAAATTGAAGAAGTAAGG
AAAAATAGGAGGAATAAAGA

Downstream 100 bases:

>100_bases
AGGGATTTGGAGGTGTTACAATGTCGAAGGTTAAACTTGTAGCTACTGGTGATGTATTATTACATTCCAGAATATACGAC
ATTAGTAAAAAGAACGGTAA

Product: hypothetical protein

Products: NA

Alternate protein names: Gamma-GCS-GS; GCS-GS; Glutamate--cysteine ligase; Gamma-ECS; GCS; Gamma-glutamylcysteine synthetase; Glutathione synthetase; GSH synthetase; GS; GSH-S; GSHase; Glutathione synthase [H]

Number of amino acids: Translated: 550; Mature: 550

Protein sequence:

>550_residues
MKERPLGISLPHLTNEITRNARKTRLDAFAVALEGWRRGLKLKWYTKDSEHFQDMIIFGVNPPGRLFSLSSEEQTHYFFR
TRGDLVSNEAVDIGSEKDDTKIYMEKAGVPIPLGKGFEPESTNEEIIEYSKTLGFPLVLKPTNASLGNGVVTNIKNEEQL
IKALHYVRTELDYKEVVVEQYVSGKEYRVYVVGDEVVAAYNRVPANIIGDGEHTIEELISMKNNVRKQNARLNSCLIHMD
VEILEFIEEAGYSLQSVPDKGKVIYLREKTNVSSGGDPVDVTDSLPEEYKKIAIDALKAVPDFPHGGVDIIVNDRGDLKG
AAVVIELNPTAQIGGALYPIEGKARDIPKAIVDYYFPETKGQVDTRKSKVYFDLITLLEPLENRAALEVEVAPAPIGELY
AKQYIVKGNVQRYNFHKWLKQQAIQHNLHGYVKSIVFDEIEIVVASTDKKTVEGFKDIIQGYPQGSSISKIKEEVYEDPI
TVGFEISEQYNTNNVRSVQHALRKMDRDLQRMRKLKNRADKDLKHILRSSSWKYTEPVRKLGGLIKTKNK

Sequences:

>Translated_550_residues
MKERPLGISLPHLTNEITRNARKTRLDAFAVALEGWRRGLKLKWYTKDSEHFQDMIIFGVNPPGRLFSLSSEEQTHYFFR
TRGDLVSNEAVDIGSEKDDTKIYMEKAGVPIPLGKGFEPESTNEEIIEYSKTLGFPLVLKPTNASLGNGVVTNIKNEEQL
IKALHYVRTELDYKEVVVEQYVSGKEYRVYVVGDEVVAAYNRVPANIIGDGEHTIEELISMKNNVRKQNARLNSCLIHMD
VEILEFIEEAGYSLQSVPDKGKVIYLREKTNVSSGGDPVDVTDSLPEEYKKIAIDALKAVPDFPHGGVDIIVNDRGDLKG
AAVVIELNPTAQIGGALYPIEGKARDIPKAIVDYYFPETKGQVDTRKSKVYFDLITLLEPLENRAALEVEVAPAPIGELY
AKQYIVKGNVQRYNFHKWLKQQAIQHNLHGYVKSIVFDEIEIVVASTDKKTVEGFKDIIQGYPQGSSISKIKEEVYEDPI
TVGFEISEQYNTNNVRSVQHALRKMDRDLQRMRKLKNRADKDLKHILRSSSWKYTEPVRKLGGLIKTKNK
>Mature_550_residues
MKERPLGISLPHLTNEITRNARKTRLDAFAVALEGWRRGLKLKWYTKDSEHFQDMIIFGVNPPGRLFSLSSEEQTHYFFR
TRGDLVSNEAVDIGSEKDDTKIYMEKAGVPIPLGKGFEPESTNEEIIEYSKTLGFPLVLKPTNASLGNGVVTNIKNEEQL
IKALHYVRTELDYKEVVVEQYVSGKEYRVYVVGDEVVAAYNRVPANIIGDGEHTIEELISMKNNVRKQNARLNSCLIHMD
VEILEFIEEAGYSLQSVPDKGKVIYLREKTNVSSGGDPVDVTDSLPEEYKKIAIDALKAVPDFPHGGVDIIVNDRGDLKG
AAVVIELNPTAQIGGALYPIEGKARDIPKAIVDYYFPETKGQVDTRKSKVYFDLITLLEPLENRAALEVEVAPAPIGELY
AKQYIVKGNVQRYNFHKWLKQQAIQHNLHGYVKSIVFDEIEIVVASTDKKTVEGFKDIIQGYPQGSSISKIKEEVYEDPI
TVGFEISEQYNTNNVRSVQHALRKMDRDLQRMRKLKNRADKDLKHILRSSSWKYTEPVRKLGGLIKTKNK

Specific function: Synthesizes glutathione from L-glutamate and L-cysteine via gamma-L-glutamyl-L-cysteine [H]

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR003806
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR007370
- InterPro:   IPR006335 [H]

Pfam domain/function: PF02655 ATP-grasp_3; PF04262 Glu_cys_ligase [H]

EC number: =6.3.2.2; =6.3.2.3 [H]

Molecular weight: Translated: 62242; Mature: 62242

Theoretical pI: Translated: 6.98; Mature: 6.98

Prosite motif: PS50975 ATP_GRASP ; PS51160 ACYLPHOSPHATASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKERPLGISLPHLTNEITRNARKTRLDAFAVALEGWRRGLKLKWYTKDSEHFQDMIIFGV
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCEEEEEEC
NPPGRLFSLSSEEQTHYFFRTRGDLVSNEAVDIGSEKDDTKIYMEKAGVPIPLGKGFEPE
CCCCCEEECCCCCCCEEEEEECCCCCCCCCEECCCCCCCCEEEEECCCCCCCCCCCCCCC
STNEEIIEYSKTLGFPLVLKPTNASLGNGVVTNIKNEEQLIKALHYVRTELDYKEVVVEQ
CCCHHHHHHHHHCCCEEEEECCCCCCCCCCEECCCCHHHHHHHHHHHHHCCCHHHHHHHH
YVSGKEYRVYVVGDEVVAAYNRVPANIIGDGEHTIEELISMKNNVRKQNARLNSCLIHMD
HHCCCCEEEEEECCHHHHHHHCCCCCEECCCHHHHHHHHHHHHHHHHHCCHHHHEEEEEC
VEILEFIEEAGYSLQSVPDKGKVIYLREKTNVSSGGDPVDVTDSLPEEYKKIAIDALKAV
HHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHC
PDFPHGGVDIIVNDRGDLKGAAVVIELNPTAQIGGALYPIEGKARDIPKAIVDYYFPETK
CCCCCCCEEEEECCCCCCCCEEEEEEECCCHHHCCEEEECCCCCHHHHHHHHHHHCCCCC
GQVDTRKSKVYFDLITLLEPLENRAALEVEVAPAPIGELYAKQYIVKGNVQRYNFHKWLK
CCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHCCCCEEHHHHHHHH
QQAIQHNLHGYVKSIVFDEIEIVVASTDKKTVEGFKDIIQGYPQGSSISKIKEEVYEDPI
HHHHHHHHHHHHHHHHHCCEEEEEECCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCC
TVGFEISEQYNTNNVRSVQHALRKMDRDLQRMRKLKNRADKDLKHILRSSSWKYTEPVRK
EEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
LGGLIKTKNK
HCCHHCCCCC
>Mature Secondary Structure
MKERPLGISLPHLTNEITRNARKTRLDAFAVALEGWRRGLKLKWYTKDSEHFQDMIIFGV
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCEEEEEEC
NPPGRLFSLSSEEQTHYFFRTRGDLVSNEAVDIGSEKDDTKIYMEKAGVPIPLGKGFEPE
CCCCCEEECCCCCCCEEEEEECCCCCCCCCEECCCCCCCCEEEEECCCCCCCCCCCCCCC
STNEEIIEYSKTLGFPLVLKPTNASLGNGVVTNIKNEEQLIKALHYVRTELDYKEVVVEQ
CCCHHHHHHHHHCCCEEEEECCCCCCCCCCEECCCCHHHHHHHHHHHHHCCCHHHHHHHH
YVSGKEYRVYVVGDEVVAAYNRVPANIIGDGEHTIEELISMKNNVRKQNARLNSCLIHMD
HHCCCCEEEEEECCHHHHHHHCCCCCEECCCHHHHHHHHHHHHHHHHHCCHHHHEEEEEC
VEILEFIEEAGYSLQSVPDKGKVIYLREKTNVSSGGDPVDVTDSLPEEYKKIAIDALKAV
HHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHC
PDFPHGGVDIIVNDRGDLKGAAVVIELNPTAQIGGALYPIEGKARDIPKAIVDYYFPETK
CCCCCCCEEEEECCCCCCCCEEEEEEECCCHHHCCEEEECCCCCHHHHHHHHHHHCCCCC
GQVDTRKSKVYFDLITLLEPLENRAALEVEVAPAPIGELYAKQYIVKGNVQRYNFHKWLK
CCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHCCCCEEHHHHHHHH
QQAIQHNLHGYVKSIVFDEIEIVVASTDKKTVEGFKDIIQGYPQGSSISKIKEEVYEDPI
HHHHHHHHHHHHHHHHHCCEEEEEECCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCC
TVGFEISEQYNTNNVRSVQHALRKMDRDLQRMRKLKNRADKDLKHILRSSSWKYTEPVRK
EEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
LGGLIKTKNK
HCCHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11248100 [H]