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The map label for this gene is hpaH [H]

Identifier: 23100327

GI number: 23100327

Start: 2956634

End: 2958130

Strand: Reverse

Name: hpaH [H]

Synonym: OB2872

Alternate gene names: 23100327

Gene position: 2958130-2956634 (Counterclockwise)

Preceding gene: 23100329

Following gene: 23100326

Centisome position: 81.48

GC content: 38.01

Gene sequence:

>1497_bases
ATGCCAGCTAAAACAGGACAGCAATACATTGATAAATTAAAAAAGGCAAATAACAACGTATATATCCATGGAGAGAGAGT
AGAAGATGTTACCACGCATCCCGCATTTAAGGGTGCCGTCCAATCCATGGCAAAATTATATGATTTACAATATGAAAAGC
CGGAAAAAATGCTTTATACATCTCCAACAACTGGTGATAAAGTGAGTAAAACTTTTATGGCACCGGAAACAATTGAAGAG
TTAATCGAACGTCGAGAAGCGATTATGGAGTGGCAGGGAATTACAAAAGGTTTGATGGGACGCTCTCCAGACTATTTAAA
TTCAGAAGTGATGACAATGGAAAAAGCTTATGATTACTACGGTAAAAGCAATTCATTATTTGCTGAAAACGCAAAGAAAT
ATGCTGAATATGCCAGAGAAAATGATATTAGCCTTACACACACATTAATTCATCCGCAAGTAAACCGTGCAAAGATTCAA
GCAGAACAAAAAGATGCAAATGTGGCTCTGCATGTAGTAGAGAAGAATGATGATGGAGTTATCGTGGACGGTATTCGCTT
ACTTGCAACCCAAGCAGGAATTACAGATGAAATTTTTGTTTTCCCGTCAACAGTAGTTAAATCAGGAGCAAAAGATGATC
CTTATTCACTTGCTTTTGCGATAGCAAATAATACGCCAGGGTTAAGATATCTAAGTCGTGAATCATTTGATTATGGTAAG
AATAACTTCGATCATCCAGTAGGTTCTCAGTTTGAAGAAGGAGATGCAATTGTTGCCTTTGATAATGTACTTGTTCCTTG
GGATCGCATCTTCATTCTAGAGGATTCTGAGATTTGTAATGGTGCGTTTGCTGAAACAAATGCTGTCGTACACATGTCCC
ATCAGGTAATCGCCAAAGATATAGCAAAAGTGGAATTTTTACTTGGTGTTGTACTTAAGCTAATGGATTCAATCGGTATT
GACGGATTTCAGCATGTGAAAGACAAAGGTACAGAAATAATGCTCGCATTAGAAAATATGAAATCGCACCTGTACAAAGC
AGAATACAATGCAAAAATTGACCGATGGGGAATGATGACGCCAGACTTTGAAGCATTGAACGCAGCAAGAAACTGGTTTC
CACGAGTATATCCGCGTTTAGCAGAAATATTAAGAGTTCTTGGTGCATCGGGATTAATGGGTATTCCAACTTATGATGAC
TTTGCTAATAAGGAACTTGGACCAATCTTAAATCGAGCTATGCAAGGTAAGAATGTGGAAGGCTTTGAACGAGTTCAATT
ATTCCGTCTTGCGTGGGATATGACGATGAGTTCATTTGGAAGCCGTCAGACGCATTATGAATACTATTTCTTTGGCGATC
CGGTCAAAATGGGTATGGCTTACTTTGACAATTATGATAAAGAGCCATACAAAGCAATGGTCGATGAGTTTTTAGAAAGC
CAAAATATTAAAAAGCCAAAAATTTTAAGCGTTCCAACAGGAAGTGTCTTAAAGTAG

Upstream 100 bases:

>100_bases
ATGCTTTATTTTTTTAGTTCTTTATGAAAGCGAATACATTTCGTGAACACTCTACCAAAATACTAGAAAAATTGGATAAA
AATTAAAGGAGGATAAAGTT

Downstream 100 bases:

>100_bases
AAATTTAATAAACGTATGTAAACTACAACTTAAGTTTAGCAGGAAGGAGAATTCTGTGATGGAAGTGATAAACCTAACTC
ATAGGGATAAACAACTATCT

Product: 4-hydroxyphenylacetate-3-hydroxylase

Products: 3,4-dihydroxyphenylacetate; NAD+; H2O

Alternate protein names: 4-HPA 3-hydroxylase; 4-hydroxyphenylacetate-3-hydroxylase [H]

Number of amino acids: Translated: 498; Mature: 497

Protein sequence:

>498_residues
MPAKTGQQYIDKLKKANNNVYIHGERVEDVTTHPAFKGAVQSMAKLYDLQYEKPEKMLYTSPTTGDKVSKTFMAPETIEE
LIERREAIMEWQGITKGLMGRSPDYLNSEVMTMEKAYDYYGKSNSLFAENAKKYAEYARENDISLTHTLIHPQVNRAKIQ
AEQKDANVALHVVEKNDDGVIVDGIRLLATQAGITDEIFVFPSTVVKSGAKDDPYSLAFAIANNTPGLRYLSRESFDYGK
NNFDHPVGSQFEEGDAIVAFDNVLVPWDRIFILEDSEICNGAFAETNAVVHMSHQVIAKDIAKVEFLLGVVLKLMDSIGI
DGFQHVKDKGTEIMLALENMKSHLYKAEYNAKIDRWGMMTPDFEALNAARNWFPRVYPRLAEILRVLGASGLMGIPTYDD
FANKELGPILNRAMQGKNVEGFERVQLFRLAWDMTMSSFGSRQTHYEYYFFGDPVKMGMAYFDNYDKEPYKAMVDEFLES
QNIKKPKILSVPTGSVLK

Sequences:

>Translated_498_residues
MPAKTGQQYIDKLKKANNNVYIHGERVEDVTTHPAFKGAVQSMAKLYDLQYEKPEKMLYTSPTTGDKVSKTFMAPETIEE
LIERREAIMEWQGITKGLMGRSPDYLNSEVMTMEKAYDYYGKSNSLFAENAKKYAEYARENDISLTHTLIHPQVNRAKIQ
AEQKDANVALHVVEKNDDGVIVDGIRLLATQAGITDEIFVFPSTVVKSGAKDDPYSLAFAIANNTPGLRYLSRESFDYGK
NNFDHPVGSQFEEGDAIVAFDNVLVPWDRIFILEDSEICNGAFAETNAVVHMSHQVIAKDIAKVEFLLGVVLKLMDSIGI
DGFQHVKDKGTEIMLALENMKSHLYKAEYNAKIDRWGMMTPDFEALNAARNWFPRVYPRLAEILRVLGASGLMGIPTYDD
FANKELGPILNRAMQGKNVEGFERVQLFRLAWDMTMSSFGSRQTHYEYYFFGDPVKMGMAYFDNYDKEPYKAMVDEFLES
QNIKKPKILSVPTGSVLK
>Mature_497_residues
PAKTGQQYIDKLKKANNNVYIHGERVEDVTTHPAFKGAVQSMAKLYDLQYEKPEKMLYTSPTTGDKVSKTFMAPETIEEL
IERREAIMEWQGITKGLMGRSPDYLNSEVMTMEKAYDYYGKSNSLFAENAKKYAEYARENDISLTHTLIHPQVNRAKIQA
EQKDANVALHVVEKNDDGVIVDGIRLLATQAGITDEIFVFPSTVVKSGAKDDPYSLAFAIANNTPGLRYLSRESFDYGKN
NFDHPVGSQFEEGDAIVAFDNVLVPWDRIFILEDSEICNGAFAETNAVVHMSHQVIAKDIAKVEFLLGVVLKLMDSIGID
GFQHVKDKGTEIMLALENMKSHLYKAEYNAKIDRWGMMTPDFEALNAARNWFPRVYPRLAEILRVLGASGLMGIPTYDDF
ANKELGPILNRAMQGKNVEGFERVQLFRLAWDMTMSSFGSRQTHYEYYFFGDPVKMGMAYFDNYDKEPYKAMVDEFLESQ
NIKKPKILSVPTGSVLK

Specific function: Utilizes FADH(2) supplied by hpaI, to catalyze the hydroxylation of 4-hydroxyphenylacetic acid, leading to the production of 3,4-dihydroxyphenylacetic acid (DHPA) [H]

COG id: COG2368

COG function: function code Q; Aromatic ring hydroxylase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FADH(2)-utilizing monooxygenase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006091
- InterPro:   IPR009075
- InterPro:   IPR009100
- InterPro:   IPR004925
- InterPro:   IPR012687 [H]

Pfam domain/function: PF03241 HpaB [H]

EC number: 1.14.13.3

Molecular weight: Translated: 56387; Mature: 56256

Theoretical pI: Translated: 5.40; Mature: 5.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPAKTGQQYIDKLKKANNNVYIHGERVEDVTTHPAFKGAVQSMAKLYDLQYEKPEKMLYT
CCCCCHHHHHHHHHHCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEE
SPTTGDKVSKTFMAPETIEELIERREAIMEWQGITKGLMGRSPDYLNSEVMTMEKAYDYY
CCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHH
GKSNSLFAENAKKYAEYARENDISLTHTLIHPQVNRAKIQAEQKDANVALHVVEKNDDGV
CCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHEEECCCCCCEEEEEEEECCCCE
IVDGIRLLATQAGITDEIFVFPSTVVKSGAKDDPYSLAFAIANNTPGLRYLSRESFDYGK
EECCHHHHHHHCCCCCEEEECCHHHHHCCCCCCCCEEEEEEECCCCCCEECCCCCCCCCC
NNFDHPVGSQFEEGDAIVAFDNVLVPWDRIFILEDSEICNGAFAETNAVVHMSHQVIAKD
CCCCCCCCCCCCCCCEEEEECCEECCCEEEEEEECCCCCCCCCCCCCEEEEEHHHHHHHH
IAKVEFLLGVVLKLMDSIGIDGFQHVKDKGTEIMLALENMKSHLYKAEYNAKIDRWGMMT
HHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCEEEEEHHHHHHHHHHHHCCCCHHHCCCCC
PDFEALNAARNWFPRVYPRLAEILRVLGASGLMGIPTYDDFANKELGPILNRAMQGKNVE
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHCCCHHHHHHHHHHCCCCCC
GFERVQLFRLAWDMTMSSFGSRQTHYEYYFFGDPVKMGMAYFDNYDKEPYKAMVDEFLES
HHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCHHHHHHHHHCCCCCCHHHHHHHHHHHH
QNIKKPKILSVPTGSVLK
CCCCCCEEEECCCCCCCC
>Mature Secondary Structure 
PAKTGQQYIDKLKKANNNVYIHGERVEDVTTHPAFKGAVQSMAKLYDLQYEKPEKMLYT
CCCCHHHHHHHHHHCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEE
SPTTGDKVSKTFMAPETIEELIERREAIMEWQGITKGLMGRSPDYLNSEVMTMEKAYDYY
CCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHH
GKSNSLFAENAKKYAEYARENDISLTHTLIHPQVNRAKIQAEQKDANVALHVVEKNDDGV
CCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHEEECCCCCCEEEEEEEECCCCE
IVDGIRLLATQAGITDEIFVFPSTVVKSGAKDDPYSLAFAIANNTPGLRYLSRESFDYGK
EECCHHHHHHHCCCCCEEEECCHHHHHCCCCCCCCEEEEEEECCCCCCEECCCCCCCCCC
NNFDHPVGSQFEEGDAIVAFDNVLVPWDRIFILEDSEICNGAFAETNAVVHMSHQVIAKD
CCCCCCCCCCCCCCCEEEEECCEECCCEEEEEEECCCCCCCCCCCCCEEEEEHHHHHHHH
IAKVEFLLGVVLKLMDSIGIDGFQHVKDKGTEIMLALENMKSHLYKAEYNAKIDRWGMMT
HHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCEEEEEHHHHHHHHHHHHCCCCHHHCCCCC
PDFEALNAARNWFPRVYPRLAEILRVLGASGLMGIPTYDDFANKELGPILNRAMQGKNVE
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHCCCHHHHHHHHHHCCCCCC
GFERVQLFRLAWDMTMSSFGSRQTHYEYYFFGDPVKMGMAYFDNYDKEPYKAMVDEFLES
HHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCHHHHHHHHHCCCCCCHHHHHHHHHHHH
QNIKKPKILSVPTGSVLK
CCCCCCEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 4-hydroxyphenylacetate; NADH; H+; O2

Specific reaction: 4-hydroxyphenylacetate + NADH + H+ + O2 = 3,4-dihydroxyphenylacetate + NAD+ + H2O

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA