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The map label for this gene is queE [H]

Identifier: 23100258

GI number: 23100258

Start: 2884471

End: 2885193

Strand: Reverse

Name: queE [H]

Synonym: OB2803

Alternate gene names: 23100258

Gene position: 2885193-2884471 (Counterclockwise)

Preceding gene: 23100259

Following gene: 23100256

Centisome position: 79.47

GC content: 36.79

Gene sequence:

>723_bases
ATGAAGATTCCCGTTTTGGAAATATTCGGTCCTACGATTCAAGGTGAAGGTATGGTTATAGGTAGAAAAACGATGTTTGT
GCGTACGGCAGGTTGTGATTACTCTTGTTCTTGGTGTGATTCTAAATTTACATGGGATGGATCGGAAAAAGACTCGATTC
AACTGTTAGAACCAGAGGAAGTTTTTGATTCATTAAAAGATATAGGTGGCAATACATTTGGTCATGTAACGATTTCAGGC
GGAAACCCTGCATTATTAAAGCAAATAGATGGATTAGTCGATCTATTACATCAACACCAAATAGAAGTCGCTTTGGAAAC
ACAGGGAAGTAGATGGCAAGATTGGTTTACAAAAGTGGATGACTTAACATTATCCCCCAAGCCTCCAAGCTCAGGAATGA
AAACTAATTTTACTACACTCGATAACATCATTGAGAGATTAGATAAAGAACAAGACGGACATTTTAGTTTGAAAGTTGTC
ATTTTTGATCAAGAGGATTTGACCTACGCAGAACAAATACATGTAAGATATCCATTTGTTACATTATTTCTACAAGTAGG
GAATGATAATATAGAAGCAAGTGAGGATGATAGATTACTATCGGACCTGCTAGATAAATATGAAAAGTTAATTGACCTTG
TGATGGAAAATAAGGTTCTAAAAGATGTTCGTGTATTACCACAATTGCACACCTATTTGTGGGGAAACAAGCGGGGCGTG
TAG

Upstream 100 bases:

>100_bases
ATTTAGATGGTCTGCAAAATAAGCCGAAGTGTATCCAAGTTTATTTACGTGAAACTCCTACCAGTTACTGTATTTATCGT
CCAAAAGGAGGGAAATCAAT

Downstream 100 bases:

>100_bases
CCCATAATAAAAAACAAGGATCGTTGTTGCCAGCGTTCCTTGTTTTTTACATTTATACGAATCTATTTAAAGAAAAAGGG
GATATATCTAAACTAGTTTC

Product: coenzyme PQQ synthesis

Products: NA

Alternate protein names: CDG synthase; Queuosine biosynthesis protein queE [H]

Number of amino acids: Translated: 240; Mature: 240

Protein sequence:

>240_residues
MKIPVLEIFGPTIQGEGMVIGRKTMFVRTAGCDYSCSWCDSKFTWDGSEKDSIQLLEPEEVFDSLKDIGGNTFGHVTISG
GNPALLKQIDGLVDLLHQHQIEVALETQGSRWQDWFTKVDDLTLSPKPPSSGMKTNFTTLDNIIERLDKEQDGHFSLKVV
IFDQEDLTYAEQIHVRYPFVTLFLQVGNDNIEASEDDRLLSDLLDKYEKLIDLVMENKVLKDVRVLPQLHTYLWGNKRGV

Sequences:

>Translated_240_residues
MKIPVLEIFGPTIQGEGMVIGRKTMFVRTAGCDYSCSWCDSKFTWDGSEKDSIQLLEPEEVFDSLKDIGGNTFGHVTISG
GNPALLKQIDGLVDLLHQHQIEVALETQGSRWQDWFTKVDDLTLSPKPPSSGMKTNFTTLDNIIERLDKEQDGHFSLKVV
IFDQEDLTYAEQIHVRYPFVTLFLQVGNDNIEASEDDRLLSDLLDKYEKLIDLVMENKVLKDVRVLPQLHTYLWGNKRGV
>Mature_240_residues
MKIPVLEIFGPTIQGEGMVIGRKTMFVRTAGCDYSCSWCDSKFTWDGSEKDSIQLLEPEEVFDSLKDIGGNTFGHVTISG
GNPALLKQIDGLVDLLHQHQIEVALETQGSRWQDWFTKVDDLTLSPKPPSSGMKTNFTTLDNIIERLDKEQDGHFSLKVV
IFDQEDLTYAEQIHVRYPFVTLFLQVGNDNIEASEDDRLLSDLLDKYEKLIDLVMENKVLKDVRVLPQLHTYLWGNKRGV

Specific function: Catalyzes the conversion of 6-carboxy-5,6,7,8- tetrahydropterin (CPH4) to 7-carboxy-7-deazaguanine (CDG) [H]

COG id: COG0602

COG function: function code O; Organic radical activating enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. 7-carboxy-7- deazaguanine synthase family [H]

Homologues:

Organism=Escherichia coli, GI1789139, Length=163, Percent_Identity=31.2883435582822, Blast_Score=71, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017742
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 27278; Mature: 27278

Theoretical pI: Translated: 4.51; Mature: 4.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIPVLEIFGPTIQGEGMVIGRKTMFVRTAGCDYSCSWCDSKFTWDGSEKDSIQLLEPEE
CCCCEEEEECCCCCCCCEEEECEEEEEEECCCCCCCCCCCCEEEECCCCCCCEEEECHHH
VFDSLKDIGGNTFGHVTISGGNPALLKQIDGLVDLLHQHQIEVALETQGSRWQDWFTKVD
HHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHEEEEEECCCCHHHHHHHHHH
DLTLSPKPPSSGMKTNFTTLDNIIERLDKEQDGHFSLKVVIFDQEDLTYAEQIHVRYPFV
CCEECCCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCEEHHEEEECCHH
TLFLQVGNDNIEASEDDRLLSDLLDKYEKLIDLVMENKVLKDVRVLPQLHTYLWGNKRGV
EEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKIPVLEIFGPTIQGEGMVIGRKTMFVRTAGCDYSCSWCDSKFTWDGSEKDSIQLLEPEE
CCCCEEEEECCCCCCCCEEEECEEEEEEECCCCCCCCCCCCEEEECCCCCCCEEEECHHH
VFDSLKDIGGNTFGHVTISGGNPALLKQIDGLVDLLHQHQIEVALETQGSRWQDWFTKVD
HHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHEEEEEECCCCHHHHHHHHHH
DLTLSPKPPSSGMKTNFTTLDNIIERLDKEQDGHFSLKVVIFDQEDLTYAEQIHVRYPFV
CCEECCCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCEEHHEEEECCHH
TLFLQVGNDNIEASEDDRLLSDLLDKYEKLIDLVMENKVLKDVRVLPQLHTYLWGNKRGV
EEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]