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The map label for this gene is nanE

Identifier: 23100250

GI number: 23100250

Start: 2874010

End: 2874678

Strand: Direct

Name: nanE

Synonym: OB2795

Alternate gene names: 23100250

Gene position: 2874010-2874678 (Clockwise)

Preceding gene: 23100246

Following gene: 23100255

Centisome position: 79.16

GC content: 34.68

Gene sequence:

>669_bases
ATGTTAGATCAAATTAAAAATAGCTTGATTGTATCTTGTCAGGCATTACCTGATGAACCATTGCATAGTTCCTTTATTAT
GTCCAAAATGGCACTTGCAGCGAAACAAGGCGGTGCAAAAGGAATTAGAGCTAATACAAAAGAAGATATTATTCAAATAA
AAGAAGAGGTTAATCTTCCTGTGGTAGGAATTGTGAAGAGAGATTATAAAGACAGTGAGGTTTTTATAACTGCTACTTAT
AAAGAAATTGATGAGCTATTAGAAAGTAATTGTGAAATGATTGCTATAGACGCGACTACAAGAAATCGCCCACAAGATAT
CAAGCTTCAAGATTTAGTGGATTATACAAAAAGATTAAATAGTGAGGTTGAGTTAATGGCAGATATCGCAACTTTAGAAG
AGGCAAAAATTGCAGAAAAGCTAGGTTTTGACTGTATCTCTACCACTCTACATGGTTATACGAGTAATACTTCTAATCAC
AAAATCTATGATAATGATTTTAGCTTTCTTAAGAACTTACTATCCGAAGTGAAAATACCAATCATTGCGGAGGGAAATAT
ACTAACCCCAGAAATGTTCAAACGATGTATTGAGCTTGGAGCTCATGCATGCGTGGTAGGTGGAGCTATCACCAGACCAC
AAGAAATAACGAAACGATTTATTGGATAA

Upstream 100 bases:

>100_bases
CATACTAAAGCTACGTTCACACCTAATTAAATATCGATTATTTTACATAGTAGATTCATAAGACACCGTTTTACTATTTA
TTAAAGGAGGATAATAAAAT

Downstream 100 bases:

>100_bases
TGACAAAAGGTGAGACAAGAGTAATTGTTCGATTCAAGCCATCCTTTATTTGAAGGTTTAGATCGAATACATGAAGAAGA
AGATGAGACAAAAGTATGTA

Product: N-acetylmannosamine-6-phosphate 2-epimerase

Products: NA

Alternate protein names: ManNAc-6-P epimerase

Number of amino acids: Translated: 222; Mature: 222

Protein sequence:

>222_residues
MLDQIKNSLIVSCQALPDEPLHSSFIMSKMALAAKQGGAKGIRANTKEDIIQIKEEVNLPVVGIVKRDYKDSEVFITATY
KEIDELLESNCEMIAIDATTRNRPQDIKLQDLVDYTKRLNSEVELMADIATLEEAKIAEKLGFDCISTTLHGYTSNTSNH
KIYDNDFSFLKNLLSEVKIPIIAEGNILTPEMFKRCIELGAHACVVGGAITRPQEITKRFIG

Sequences:

>Translated_222_residues
MLDQIKNSLIVSCQALPDEPLHSSFIMSKMALAAKQGGAKGIRANTKEDIIQIKEEVNLPVVGIVKRDYKDSEVFITATY
KEIDELLESNCEMIAIDATTRNRPQDIKLQDLVDYTKRLNSEVELMADIATLEEAKIAEKLGFDCISTTLHGYTSNTSNH
KIYDNDFSFLKNLLSEVKIPIIAEGNILTPEMFKRCIELGAHACVVGGAITRPQEITKRFIG
>Mature_222_residues
MLDQIKNSLIVSCQALPDEPLHSSFIMSKMALAAKQGGAKGIRANTKEDIIQIKEEVNLPVVGIVKRDYKDSEVFITATY
KEIDELLESNCEMIAIDATTRNRPQDIKLQDLVDYTKRLNSEVELMADIATLEEAKIAEKLGFDCISTTLHGYTSNTSNH
KIYDNDFSFLKNLLSEVKIPIIAEGNILTPEMFKRCIELGAHACVVGGAITRPQEITKRFIG

Specific function: Converts N-acetylmannosamine-6-phosphate (ManNAc-6-P) to N-acetylglucosamine-6-phosphate (GlcNAc-6-P) (Potential)

COG id: COG3010

COG function: function code G; Putative N-acetylmannosamine-6-phosphate epimerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nanE family

Homologues:

Organism=Escherichia coli, GI1789617, Length=223, Percent_Identity=38.1165919282511, Blast_Score=117, Evalue=8e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NANE_OCEIH (Q8EMP6)

Other databases:

- EMBL:   BA000028
- RefSeq:   NP_693717.1
- ProteinModelPortal:   Q8EMP6
- SMR:   Q8EMP6
- GeneID:   1016970
- GenomeReviews:   BA000028_GR
- KEGG:   oih:OB2795
- NMPDR:   fig|221109.1.peg.2797
- HOGENOM:   HBG296941
- OMA:   TRPQEIT
- ProtClustDB:   PRK01130
- BioCyc:   OIHE221109:OB2795-MONOMER
- BRENDA:   5.1.3.9
- HAMAP:   MF_01235
- InterPro:   IPR013785
- InterPro:   IPR007260
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70

Pfam domain/function: PF04131 NanE; SSF51366 RibP_bind_barrel

EC number: =5.1.3.9

Molecular weight: Translated: 24735; Mature: 24735

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDQIKNSLIVSCQALPDEPLHSSFIMSKMALAAKQGGAKGIRANTKEDIIQIKEEVNLP
CCHHHHHHHEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCC
VVGIVKRDYKDSEVFITATYKEIDELLESNCEMIAIDATTRNRPQDIKLQDLVDYTKRLN
EEEEEECCCCCCEEEEEEHHHHHHHHHHCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHC
SEVELMADIATLEEAKIAEKLGFDCISTTLHGYTSNTSNHKIYDNDFSFLKNLLSEVKIP
CHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCCCCCCCEEECCHHHHHHHHHHHCCCC
IIAEGNILTPEMFKRCIELGAHACVVGGAITRPQEITKRFIG
EEECCCEECHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHCC
>Mature Secondary Structure
MLDQIKNSLIVSCQALPDEPLHSSFIMSKMALAAKQGGAKGIRANTKEDIIQIKEEVNLP
CCHHHHHHHEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCC
VVGIVKRDYKDSEVFITATYKEIDELLESNCEMIAIDATTRNRPQDIKLQDLVDYTKRLN
EEEEEECCCCCCEEEEEEHHHHHHHHHHCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHC
SEVELMADIATLEEAKIAEKLGFDCISTTLHGYTSNTSNHKIYDNDFSFLKNLLSEVKIP
CHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCCCCCCCEEECCHHHHHHHHHHHCCCC
IIAEGNILTPEMFKRCIELGAHACVVGGAITRPQEITKRFIG
EEECCCEECHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12235376