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The map label for this gene is yckB [H]

Identifier: 23100188

GI number: 23100188

Start: 2801519

End: 2802364

Strand: Reverse

Name: yckB [H]

Synonym: OB2733

Alternate gene names: 23100188

Gene position: 2802364-2801519 (Counterclockwise)

Preceding gene: 23100191

Following gene: 23100185

Centisome position: 77.19

GC content: 37.94

Gene sequence:

>846_bases
TTGAAAGGAATAAAATCAATCCTTACTTTAATTTTAATTTTAATACTAAGTGCATGTGGCAGTTCCAATAATGAGGAAGG
AAATGCAAGCGACGAGCGATGGGAAGAAATTAATACAAGTGGAGAAATTGTAGTTGGTACATCTGGTACGTTATTTCCTG
CATCTTATTATGAAGGGGATAAACTAACTGGATTCGATGTTGAAGTGATGCGAGAAGTTGCCAAGAGATTGAATTTAGAT
GTTACCTTTGAAACTATGGGCGTCGATAGCATGCTGTCAGCAATTCAAAGCGGAAGAATTGATGTTGCAGCCAATCAAAT
CGAACCAACGGAGAAACGTCAGAAGGAATTTAATTTCAGTGAACCATATAAATATTCGTATACTACGATGGTAGTGCGTA
AAGGCGATAATTCTGGAATCAATTCATTAGAAGATTTAGAGGGGAAAAAAGCTGGCGGTGGAGCAACAACCGTCTATAGT
CAGATTGCAGAGCATTTTGGAGCAGAAGTGGTTACTTATGGAAATGCGCCCAATGAAGCATATTTACGTGATGTACATAA
TGGAAGAACAGACGTAGTAATCAATGATTATTATCTGCAGAAATTTGGGATTGCCGAATTTCCTGAATTTGATATCCATC
TCCATCCGGAATTACGATTTAGAACTACGGAGCAATCAATTGTGATCCCCAATGGTGAAACGAAGCTAACAACCGAAATA
AATCAAGTTCTCGAAGAAATGCGCAATGATGGGACACTATCCGATTTGGCCATTCAATATTTTGAGGAAGATGCTTCTAA
AAAGCCTAATTTAGAAATGGATGAGATAGAGGGGCTGGATTTATAA

Upstream 100 bases:

>100_bases
ATAAGCATTTAGAAATTCGGTGATGAAACATTTTGACAACACTAATTATTTTAAGTAGAGTTTTGTTGTAATAAAAAAAA
GTGAAAGTAGGTGTATATGT

Downstream 100 bases:

>100_bases
TGGTCTTCACAGAAGTAAATTAAAACCTAAGGTGAAAGATTCCAGGTATCGTCTTGATGACGATACCTGGAATCTTTTTT
AGTCATTAATTAATTTTGAT

Product: amino acid ABC transporter substrate-binding protein

Products: NA

Alternate protein names: ORF2 [H]

Number of amino acids: Translated: 281; Mature: 281

Protein sequence:

>281_residues
MKGIKSILTLILILILSACGSSNNEEGNASDERWEEINTSGEIVVGTSGTLFPASYYEGDKLTGFDVEVMREVAKRLNLD
VTFETMGVDSMLSAIQSGRIDVAANQIEPTEKRQKEFNFSEPYKYSYTTMVVRKGDNSGINSLEDLEGKKAGGGATTVYS
QIAEHFGAEVVTYGNAPNEAYLRDVHNGRTDVVINDYYLQKFGIAEFPEFDIHLHPELRFRTTEQSIVIPNGETKLTTEI
NQVLEEMRNDGTLSDLAIQYFEEDASKKPNLEMDEIEGLDL

Sequences:

>Translated_281_residues
MKGIKSILTLILILILSACGSSNNEEGNASDERWEEINTSGEIVVGTSGTLFPASYYEGDKLTGFDVEVMREVAKRLNLD
VTFETMGVDSMLSAIQSGRIDVAANQIEPTEKRQKEFNFSEPYKYSYTTMVVRKGDNSGINSLEDLEGKKAGGGATTVYS
QIAEHFGAEVVTYGNAPNEAYLRDVHNGRTDVVINDYYLQKFGIAEFPEFDIHLHPELRFRTTEQSIVIPNGETKLTTEI
NQVLEEMRNDGTLSDLAIQYFEEDASKKPNLEMDEIEGLDL
>Mature_281_residues
MKGIKSILTLILILILSACGSSNNEEGNASDERWEEINTSGEIVVGTSGTLFPASYYEGDKLTGFDVEVMREVAKRLNLD
VTFETMGVDSMLSAIQSGRIDVAANQIEPTEKRQKEFNFSEPYKYSYTTMVVRKGDNSGINSLEDLEGKKAGGGATTVYS
QIAEHFGAEVVTYGNAPNEAYLRDVHNGRTDVVINDYYLQKFGIAEFPEFDIHLHPELRFRTTEQSIVIPNGETKLTTEI
NQVLEEMRNDGTLSDLAIQYFEEDASKKPNLEMDEIEGLDL

Specific function: Probably part of a binding-protein-dependent transport system [H]

COG id: COG0834

COG function: function code ET; ABC-type amino acid transport/signal transduction systems, periplasmic component/domain

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788228, Length=236, Percent_Identity=31.7796610169492, Blast_Score=120, Evalue=9e-29,
Organism=Escherichia coli, GI1787031, Length=267, Percent_Identity=26.9662921348315, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI1788648, Length=233, Percent_Identity=28.3261802575107, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1788649, Length=268, Percent_Identity=25.3731343283582, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1787085, Length=225, Percent_Identity=23.1111111111111, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1787088, Length=209, Percent_Identity=23.9234449760766, Blast_Score=68, Evalue=5e-13,

Paralogues:

None

Copy number: 1920 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1060 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 80 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015683
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 31307; Mature: 31307

Theoretical pI: Translated: 4.20; Mature: 4.20

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS01039 SBP_BACTERIAL_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKGIKSILTLILILILSACGSSNNEEGNASDERWEEINTSGEIVVGTSGTLFPASYYEGD
CCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCCCCEEECCCCCCCCCCCCCCC
KLTGFDVEVMREVAKRLNLDVTFETMGVDSMLSAIQSGRIDVAANQIEPTEKRQKEFNFS
CCCCCCHHHHHHHHHHCCCCEEEECCCHHHHHHHHHCCCEEEEECCCCCHHHHHHHCCCC
EPYKYSYTTMVVRKGDNSGINSLEDLEGKKAGGGATTVYSQIAEHFGAEVVTYGNAPNEA
CCCCEEEEEEEEECCCCCCCCCHHHHCCCCCCCCHHHHHHHHHHHHCCEEEEECCCCCHH
YLRDVHNGRTDVVINDYYLQKFGIAEFPEFDIHLHPELRFRTTEQSIVIPNGETKLTTEI
HHHHHCCCCCEEEEEHHHHHHCCCCCCCCCCEEECCCEEEEECCCEEEECCCCCCHHHHH
NQVLEEMRNDGTLSDLAIQYFEEDASKKPNLEMDEIEGLDL
HHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCHHCCCCCCC
>Mature Secondary Structure
MKGIKSILTLILILILSACGSSNNEEGNASDERWEEINTSGEIVVGTSGTLFPASYYEGD
CCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCCCCEEECCCCCCCCCCCCCCC
KLTGFDVEVMREVAKRLNLDVTFETMGVDSMLSAIQSGRIDVAANQIEPTEKRQKEFNFS
CCCCCCHHHHHHHHHHCCCCEEEECCCHHHHHHHHHCCCEEEEECCCCCHHHHHHHCCCC
EPYKYSYTTMVVRKGDNSGINSLEDLEGKKAGGGATTVYSQIAEHFGAEVVTYGNAPNEA
CCCCEEEEEEEEECCCCCCCCCHHHHCCCCCCCCHHHHHHHHHHHHCCEEEEECCCCCHH
YLRDVHNGRTDVVINDYYLQKFGIAEFPEFDIHLHPELRFRTTEQSIVIPNGETKLTTEI
HHHHHCCCCCEEEEEHHHHHHCCCCCCCCCCEEECCCEEEEECCCEEEECCCCCCHHHHH
NQVLEEMRNDGTLSDLAIQYFEEDASKKPNLEMDEIEGLDL
HHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7704255; 9384377 [H]