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The map label for this gene is amhX [H]

Identifier: 23100164

GI number: 23100164

Start: 2779095

End: 2780216

Strand: Reverse

Name: amhX [H]

Synonym: OB2709

Alternate gene names: 23100164

Gene position: 2780216-2779095 (Counterclockwise)

Preceding gene: 23100165

Following gene: 23100163

Centisome position: 76.58

GC content: 38.68

Gene sequence:

>1122_bases
ATGACAACGAGTATTGAACAAAGAGTAAGAGATGTGTTTCATTATTTACACCAACATCCTGAAATTAGTTGGGAAGAGGT
GGAAACTACCAAGTATATCAAGCAGCAACTAGAAAAAGCAGGGTGTAATGTGACCACCTTTACGGATTGTACAGGGGTAA
TTGGAGAATATGGAGATTTTAATAAGGGACTTCCGGTTGTAGGTATTCGTGCAGATATGGATGCACTATGGCAAGAAGTG
AATGGGATTTTTCAAGCGAACCATTCTTGTGGACATGATGCACATATGGCAATGGTCCTTGGTGTACTATGGTCATTACA
AGAAATTCCAAATATGAAAGACAAGATAGCGATTAAGTTTATCTTTCAACCAGCTGAAGAAAATGGTAGCGGTGCACTTA
AAATGGTAGAAAAAGGTGTGGTAGACAACGTCGACTATTTATTCGGTGTACATCTTCGTCCAGACCAAGAAGCGAAGATG
GGGACAGCAACTCCGGTTATTGTACATGGTGCCACTAGAATGTTTCATGGTGAGATTCAAGGAGACGATGCGCATGGAGC
TAGACCACATCTAAATCATAATGCAATCGAAGTCGGAGCCCAAATCGTTAATATGATCCGTCAAATTCACTTAGATCCAC
GTATTCCTCATTCTGTAAAAATGACAAAATTTCAGTCCGGTGGAAAGAATGCTAATATTATTCCCGGAAATGCTGCATTT
AGCCTTGATTTACGAGCGCAATCAAATATGGTAATGGAGAATTTGGAAAGAAAGGTTCATAATATCTTTGATGCAGTTCG
AGATTTATATGATATTAAAATTGAAATAACAGATGTACATGGGATTGCTGCAGCAGAAATTAATGAAGAAGCTATCGATA
TTATGCAAAGTGCTATTACTTCTCATCTAGGAGAGGAGAAATTGCTCGAACCATTAATAACACCTGGTGGGGATGACTTC
CATTTTTATACTATTAAACGTCCAGAACTGAAAGCCACGATGCTTGGTTTAGGCTGTTCACTAACTCCTGGTCTGCATCA
TCCGACAATGACATTTGATCAACATGCACTTGAAAATGGAGTGGGAATTCTAACAAAAGCAGTATTAGATGTATATAAAT
AG

Upstream 100 bases:

>100_bases
CTCTGATGAATTCAATCTTATCTAAATTGATCGATCAGGTTCAAGATCGAGGTAAAATATTACCGACATTTAAGTATTAT
ATAAAGGACGGGGAAGATGA

Downstream 100 bases:

>100_bases
AAAATTTATGCTGTCCCTATCTTAGAGATATATTCTAGATAGGGATTTTTTACATTGATATTTTATTCATTAACTAAATC
GAGCGCCTTCTCAACATGAG

Product: amidohydrolase

Products: NA

Alternate protein names: Aminoacylase [H]

Number of amino acids: Translated: 373; Mature: 372

Protein sequence:

>373_residues
MTTSIEQRVRDVFHYLHQHPEISWEEVETTKYIKQQLEKAGCNVTTFTDCTGVIGEYGDFNKGLPVVGIRADMDALWQEV
NGIFQANHSCGHDAHMAMVLGVLWSLQEIPNMKDKIAIKFIFQPAEENGSGALKMVEKGVVDNVDYLFGVHLRPDQEAKM
GTATPVIVHGATRMFHGEIQGDDAHGARPHLNHNAIEVGAQIVNMIRQIHLDPRIPHSVKMTKFQSGGKNANIIPGNAAF
SLDLRAQSNMVMENLERKVHNIFDAVRDLYDIKIEITDVHGIAAAEINEEAIDIMQSAITSHLGEEKLLEPLITPGGDDF
HFYTIKRPELKATMLGLGCSLTPGLHHPTMTFDQHALENGVGILTKAVLDVYK

Sequences:

>Translated_373_residues
MTTSIEQRVRDVFHYLHQHPEISWEEVETTKYIKQQLEKAGCNVTTFTDCTGVIGEYGDFNKGLPVVGIRADMDALWQEV
NGIFQANHSCGHDAHMAMVLGVLWSLQEIPNMKDKIAIKFIFQPAEENGSGALKMVEKGVVDNVDYLFGVHLRPDQEAKM
GTATPVIVHGATRMFHGEIQGDDAHGARPHLNHNAIEVGAQIVNMIRQIHLDPRIPHSVKMTKFQSGGKNANIIPGNAAF
SLDLRAQSNMVMENLERKVHNIFDAVRDLYDIKIEITDVHGIAAAEINEEAIDIMQSAITSHLGEEKLLEPLITPGGDDF
HFYTIKRPELKATMLGLGCSLTPGLHHPTMTFDQHALENGVGILTKAVLDVYK
>Mature_372_residues
TTSIEQRVRDVFHYLHQHPEISWEEVETTKYIKQQLEKAGCNVTTFTDCTGVIGEYGDFNKGLPVVGIRADMDALWQEVN
GIFQANHSCGHDAHMAMVLGVLWSLQEIPNMKDKIAIKFIFQPAEENGSGALKMVEKGVVDNVDYLFGVHLRPDQEAKMG
TATPVIVHGATRMFHGEIQGDDAHGARPHLNHNAIEVGAQIVNMIRQIHLDPRIPHSVKMTKFQSGGKNANIIPGNAAFS
LDLRAQSNMVMENLERKVHNIFDAVRDLYDIKIEITDVHGIAAAEINEEAIDIMQSAITSHLGEEKLLEPLITPGGDDFH
FYTIKRPELKATMLGLGCSLTPGLHHPTMTFDQHALENGVGILTKAVLDVYK

Specific function: Required But Not Essential For Aminobenzoyl-Glutamate Utilization. May Participate In Hydrolysis Of Aminobenzoyl- Glutamate To Aminobenzoate, Either Alone Or In Combination With Abgb. [C]

COG id: COG1473

COG function: function code R; Metal-dependent amidase/aminoacylase/carboxypeptidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20 family [H]

Homologues:

Organism=Escherichia coli, GI87081880, Length=349, Percent_Identity=27.7936962750716, Blast_Score=94, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017439
- InterPro:   IPR010168
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: NA

Molecular weight: Translated: 41394; Mature: 41263

Theoretical pI: Translated: 5.94; Mature: 5.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTSIEQRVRDVFHYLHQHPEISWEEVETTKYIKQQLEKAGCNVTTFTDCTGVIGEYGDF
CCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHCCCCCC
NKGLPVVGIRADMDALWQEVNGIFQANHSCGHDAHMAMVLGVLWSLQEIPNMKDKIAIKF
CCCCCEEEEECCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHEEEEE
IFQPAEENGSGALKMVEKGVVDNVDYLFGVHLRPDQEAKMGTATPVIVHGATRMFHGEIQ
EEECCCCCCCCHHHHHHHCCCCCCHHHEEEECCCCCCCCCCCCCEEEEECCHHEEECEEC
GDDAHGARPHLNHNAIEVGAQIVNMIRQIHLDPRIPHSVKMTKFQSGGKNANIIPGNAAF
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCEECCCCEE
SLDLRAQSNMVMENLERKVHNIFDAVRDLYDIKIEITDVHGIAAAEINEEAIDIMQSAIT
EEEECCHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEECCCEEHHHCCHHHHHHHHHHHH
SHLGEEKLLEPLITPGGDDFHFYTIKRPELKATMLGLGCSLTPGLHHPTMTFDQHALENG
HHCCHHHHHHHHCCCCCCCEEEEEEECCCHHHHHEECCCCCCCCCCCCCCCHHHHHHHCC
VGILTKAVLDVYK
HHHHHHHHHHHCC
>Mature Secondary Structure 
TTSIEQRVRDVFHYLHQHPEISWEEVETTKYIKQQLEKAGCNVTTFTDCTGVIGEYGDF
CCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHCCCCCC
NKGLPVVGIRADMDALWQEVNGIFQANHSCGHDAHMAMVLGVLWSLQEIPNMKDKIAIKF
CCCCCEEEEECCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHEEEEE
IFQPAEENGSGALKMVEKGVVDNVDYLFGVHLRPDQEAKMGTATPVIVHGATRMFHGEIQ
EEECCCCCCCCHHHHHHHCCCCCCHHHEEEECCCCCCCCCCCCCEEEEECCHHEEECEEC
GDDAHGARPHLNHNAIEVGAQIVNMIRQIHLDPRIPHSVKMTKFQSGGKNANIIPGNAAF
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCEECCCCEE
SLDLRAQSNMVMENLERKVHNIFDAVRDLYDIKIEITDVHGIAAAEINEEAIDIMQSAIT
EEEECCHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEECCCEEHHHCCHHHHHHHHHHHH
SHLGEEKLLEPLITPGGDDFHFYTIKRPELKATMLGLGCSLTPGLHHPTMTFDQHALENG
HHCCHHHHHHHHCCCCCCCEEEEEEECCCHHHHHEECCCCCCCCCCCCCCCHHHHHHHCC
VGILTKAVLDVYK
HHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8768514; 8969502; 9384377; 7622480 [H]