Click here to switch to the map view.

The map label for this gene is kipI [H]

Identifier: 23100133

GI number: 23100133

Start: 2749070

End: 2749783

Strand: Reverse

Name: kipI [H]

Synonym: OB2678

Alternate gene names: 23100133

Gene position: 2749783-2749070 (Counterclockwise)

Preceding gene: 23100134

Following gene: 23100132

Centisome position: 75.74

GC content: 36.55

Gene sequence:

>714_bases
ATGTTTGAGGTTCAACCCTATGGGGATAGAGGAGTAAGAGTTCAATTTGGGGAGTCTATTGATAAGAATGTCAACAGGCA
GATACGAGATTTTTGTTCTGTTTTAGAAAAGTCACGTATTAATGGCGTTCTTGAATGGATTCCTACATATACAGCAGTAA
CGATTTTGTATGATCCGTTTGAAATTGTTTATGAATCATTGAAGGAAAAAATTTTCAATGTACAAGAAAAAATGAATAGT
GAAGATATTTCTCCGGCTCGCATCATTCATATTCCTGTACTTTATGGTGGCAAAAAAGGTCCGGATTTAAATGAAGTGGC
AGAATATCATGGGATAACTACAGATGAAGTAGTGTCACTTCATGCTAATTCCGATTATCTTATCTATATGATGGGATTTA
TACCTGGTTTTCCGTATCTTGGAGGACTTTCAAAGCAAATTGCTACTCCTAGGTTAGATAAACCAAGGAGGAGTATTCCA
GCAGGTAGTGTTGGAATAGCAGGAGAACAAACAGGGATTTATCCATTGGAGTCACCAGGTGGTTGGAGAATTATTGGTCA
GACTCCAATTCCTTTATATAATCCACAACAAGCACAGCCTATTTTATTAAAAGCAGGAGATTATATTCGTTTTATACCAA
TTGACCGTCAAGAATTTGACGATATTACAAAAAAGGTGGAGACAGGAAGTTTTCACCCTACGATAGAGACTTAA

Upstream 100 bases:

>100_bases
TTGTAGTTATCCTGACTACGTATTTAGGCATCCAAACATTAATCACTCAAATTCCGCAATTATTTAATATGTAAAATTCA
CAGAAAGTGGTTGGTTCAAA

Downstream 100 bases:

>100_bases
ATCTTAGGGGGATATGACGAATGCAATATCAAGTAGATTTAAATAGTGACATCGGTGAAAGTTATGGTGCTTATACGATT
GGTCAAGATGATGAGGTAAT

Product: hypothetical protein

Products: NA

Alternate protein names: Sporulation inhibitor kipI [H]

Number of amino acids: Translated: 237; Mature: 237

Protein sequence:

>237_residues
MFEVQPYGDRGVRVQFGESIDKNVNRQIRDFCSVLEKSRINGVLEWIPTYTAVTILYDPFEIVYESLKEKIFNVQEKMNS
EDISPARIIHIPVLYGGKKGPDLNEVAEYHGITTDEVVSLHANSDYLIYMMGFIPGFPYLGGLSKQIATPRLDKPRRSIP
AGSVGIAGEQTGIYPLESPGGWRIIGQTPIPLYNPQQAQPILLKAGDYIRFIPIDRQEFDDITKKVETGSFHPTIET

Sequences:

>Translated_237_residues
MFEVQPYGDRGVRVQFGESIDKNVNRQIRDFCSVLEKSRINGVLEWIPTYTAVTILYDPFEIVYESLKEKIFNVQEKMNS
EDISPARIIHIPVLYGGKKGPDLNEVAEYHGITTDEVVSLHANSDYLIYMMGFIPGFPYLGGLSKQIATPRLDKPRRSIP
AGSVGIAGEQTGIYPLESPGGWRIIGQTPIPLYNPQQAQPILLKAGDYIRFIPIDRQEFDDITKKVETGSFHPTIET
>Mature_237_residues
MFEVQPYGDRGVRVQFGESIDKNVNRQIRDFCSVLEKSRINGVLEWIPTYTAVTILYDPFEIVYESLKEKIFNVQEKMNS
EDISPARIIHIPVLYGGKKGPDLNEVAEYHGITTDEVVSLHANSDYLIYMMGFIPGFPYLGGLSKQIATPRLDKPRRSIP
AGSVGIAGEQTGIYPLESPGGWRIIGQTPIPLYNPQQAQPILLKAGDYIRFIPIDRQEFDDITKKVETGSFHPTIET

Specific function: Is a potent inhibitor of the autophosphorylation reaction of kinase A and its reverse reaction, but does not inhibit phosphate transfer to the Spo0F response regulator once kinase A is phosphorylated. KipI is an inhibitor of the catalytic domain of kinase

COG id: COG2049

COG function: function code E; Allophanate hydrolase subunit 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786929, Length=173, Percent_Identity=46.8208092485549, Blast_Score=160, Evalue=6e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003833
- InterPro:   IPR020899
- InterPro:   IPR010016
- InterPro:   IPR002130 [H]

Pfam domain/function: PF02682 AHS1 [H]

EC number: NA

Molecular weight: Translated: 26691; Mature: 26691

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFEVQPYGDRGVRVQFGESIDKNVNRQIRDFCSVLEKSRINGVLEWIPTYTAVTILYDPF
CCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCH
EIVYESLKEKIFNVQEKMNSEDISPARIIHIPVLYGGKKGPDLNEVAEYHGITTDEVVSL
HHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEEECCCCCCCHHHHHHHCCCCHHHEEEE
HANSDYLIYMMGFIPGFPYLGGLSKQIATPRLDKPRRSIPAGSVGIAGEQTGIYPLESPG
ECCCCEEEEEEHHCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCEECCCCC
GWRIIGQTPIPLYNPQQAQPILLKAGDYIRFIPIDRQEFDDITKKVETGSFHPTIET
CEEEECCCCCCCCCCCCCCEEEEECCCEEEEEECCHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MFEVQPYGDRGVRVQFGESIDKNVNRQIRDFCSVLEKSRINGVLEWIPTYTAVTILYDPF
CCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCH
EIVYESLKEKIFNVQEKMNSEDISPARIIHIPVLYGGKKGPDLNEVAEYHGITTDEVVSL
HHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEEECCCCCCCHHHHHHHCCCCHHHEEEE
HANSDYLIYMMGFIPGFPYLGGLSKQIATPRLDKPRRSIPAGSVGIAGEQTGIYPLESPG
ECCCCEEEEEEHHCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCEECCCCC
GWRIIGQTPIPLYNPQQAQPILLKAGDYIRFIPIDRQEFDDITKKVETGSFHPTIET
CEEEECCCCCCCCCCCCCCEEEEECCCEEEEEECCHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969502; 9384377; 10568751 [H]