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The map label for this gene is rfbB [H]

Identifier: 23099875

GI number: 23099875

Start: 2474354

End: 2475391

Strand: Reverse

Name: rfbB [H]

Synonym: OB2420

Alternate gene names: 23099875

Gene position: 2475391-2474354 (Counterclockwise)

Preceding gene: 23099876

Following gene: 23099874

Centisome position: 68.18

GC content: 35.65

Gene sequence:

>1038_bases
ATGAATAAGTCAGCAATTTTAATAACAGGTGGAGCTGGATTTATTGGATCAAATTTTATCCATTACTTTATGGATGTATA
TCCGGAAGTTCCGATTGTTAATATAGATAAATTAACTTATGCTGGATCTCTTCAGAATCTTAAAGGAATAGATTCAAACT
CTAACTATCATTTTGTAGAAGGTGATATTGCAGATGAACAGGTTGTACAGCATGTATTTCAAAATTTTGATATCCACGGA
GTCATTCATTTTGCAGCCGAATCACATGTGGATAGGTCTATTGAAGATGCGCGTTCTTTTGTAGATACGAATGTTGTCGG
CACGCTGAACTTACTACAAGCTGCACGTAATGATTGGGAAGAAAAAGGGGAACTAGAAGGCAGGCGTTTTCATCATATAT
CAACTGACGAAGTATATGGTTCACTTGGAGAAGAAGGAATGTTTACTGAAGAAACTCCTTATGATCCTAGAAACCCATAT
AGTGCGTCAAAGGCTGGTGCAAACCTGATGGTGAAAAGTTTTGGGTATACGTATGGTATGAATGTTATTTTATCTTCAAG
TTCGAATAACTATGGACCTAGACAGCATCAAGAGAAGTTGTTACCTACAATTATTGAAAAAGCGTTAAGCGGCAAGGAAA
TTCCGATTTATGGTGACGGAAAAAATGTTCGAGATTGGTTATATGTAGAAGATCATTGCCGGGCAATTGATACGGTATAT
CATTTTGGGAAATCTCAGGAGACATATAACGTCGGTGGTAGAAACGAAAGAACTAACCTTGAAATGACAAATCAGATTTG
TGATATTCTAAATGATTTACGTCCTGATTTATTATCCAAGTATGAATTGGAGAAGTTTCAAGATTTGATTACATTTGTCG
AAGATCGTAGAGGACACGATTTACGGTATGCAATTGACGATCGTAAATTAAGAAATGAATTACAATGGGAACCAAAACAA
ACATTAGAGAATGGACTGAAACAAACTATTGAGTGGTATATAAGAAAATGGGAATCTCCTACAAAAATGAACCAGTAA

Upstream 100 bases:

>100_bases
AATGATTACGGTCAATATCTTATGGAAATTGCCGATAGAAAGCATCATCAGCAATACTGGGATTCGATCGATCATCATCC
AATGTTAGGACTGGTTGAAA

Downstream 100 bases:

>100_bases
TTTCAGTTGTAGTACCTGTTTATGGTTGCAAAACCTGCCTTGTAGAATTATGTAGTCGTATTAAAGAGACGGTACTGTCC
ATTCCAGCTCAATATGAAAT

Product: spore coat polysaccharide synthesis

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 345; Mature: 345

Protein sequence:

>345_residues
MNKSAILITGGAGFIGSNFIHYFMDVYPEVPIVNIDKLTYAGSLQNLKGIDSNSNYHFVEGDIADEQVVQHVFQNFDIHG
VIHFAAESHVDRSIEDARSFVDTNVVGTLNLLQAARNDWEEKGELEGRRFHHISTDEVYGSLGEEGMFTEETPYDPRNPY
SASKAGANLMVKSFGYTYGMNVILSSSSNNYGPRQHQEKLLPTIIEKALSGKEIPIYGDGKNVRDWLYVEDHCRAIDTVY
HFGKSQETYNVGGRNERTNLEMTNQICDILNDLRPDLLSKYELEKFQDLITFVEDRRGHDLRYAIDDRKLRNELQWEPKQ
TLENGLKQTIEWYIRKWESPTKMNQ

Sequences:

>Translated_345_residues
MNKSAILITGGAGFIGSNFIHYFMDVYPEVPIVNIDKLTYAGSLQNLKGIDSNSNYHFVEGDIADEQVVQHVFQNFDIHG
VIHFAAESHVDRSIEDARSFVDTNVVGTLNLLQAARNDWEEKGELEGRRFHHISTDEVYGSLGEEGMFTEETPYDPRNPY
SASKAGANLMVKSFGYTYGMNVILSSSSNNYGPRQHQEKLLPTIIEKALSGKEIPIYGDGKNVRDWLYVEDHCRAIDTVY
HFGKSQETYNVGGRNERTNLEMTNQICDILNDLRPDLLSKYELEKFQDLITFVEDRRGHDLRYAIDDRKLRNELQWEPKQ
TLENGLKQTIEWYIRKWESPTKMNQ
>Mature_345_residues
MNKSAILITGGAGFIGSNFIHYFMDVYPEVPIVNIDKLTYAGSLQNLKGIDSNSNYHFVEGDIADEQVVQHVFQNFDIHG
VIHFAAESHVDRSIEDARSFVDTNVVGTLNLLQAARNDWEEKGELEGRRFHHISTDEVYGSLGEEGMFTEETPYDPRNPY
SASKAGANLMVKSFGYTYGMNVILSSSSNNYGPRQHQEKLLPTIIEKALSGKEIPIYGDGKNVRDWLYVEDHCRAIDTVY
HFGKSQETYNVGGRNERTNLEMTNQICDILNDLRPDLLSKYELEKFQDLITFVEDRRGHDLRYAIDDRKLRNELQWEPKQ
TLENGLKQTIEWYIRKWESPTKMNQ

Specific function: Catalyzes the dehydration of dTDP-D-glucose to form dTDP-6-deoxy-D-xylo-4-hexulose via a three-step process involving oxidation, dehydration and reduction [H]

COG id: COG1088

COG function: function code M; dTDP-D-glucose 4,6-dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=329, Percent_Identity=38.9057750759878, Blast_Score=226, Evalue=3e-59,
Organism=Homo sapiens, GI42516563, Length=343, Percent_Identity=24.7813411078717, Blast_Score=98, Evalue=1e-20,
Organism=Homo sapiens, GI4504031, Length=244, Percent_Identity=27.0491803278689, Blast_Score=72, Evalue=8e-13,
Organism=Escherichia coli, GI48994969, Length=332, Percent_Identity=48.7951807228916, Blast_Score=331, Evalue=3e-92,
Organism=Escherichia coli, GI1788353, Length=345, Percent_Identity=46.0869565217391, Blast_Score=317, Evalue=8e-88,
Organism=Escherichia coli, GI1788366, Length=247, Percent_Identity=29.1497975708502, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI1786974, Length=360, Percent_Identity=25.5555555555556, Blast_Score=75, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI17568069, Length=337, Percent_Identity=36.4985163204748, Blast_Score=209, Evalue=2e-54,
Organism=Caenorhabditis elegans, GI115532424, Length=336, Percent_Identity=33.9285714285714, Blast_Score=182, Evalue=2e-46,
Organism=Caenorhabditis elegans, GI17539532, Length=338, Percent_Identity=24.8520710059172, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI133901786, Length=190, Percent_Identity=27.3684210526316, Blast_Score=69, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17539422, Length=190, Percent_Identity=27.3684210526316, Blast_Score=69, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI133901788, Length=190, Percent_Identity=27.3684210526316, Blast_Score=69, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI133901790, Length=190, Percent_Identity=27.3684210526316, Blast_Score=69, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17539424, Length=190, Percent_Identity=27.3684210526316, Blast_Score=69, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI71982035, Length=278, Percent_Identity=26.6187050359712, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI71982038, Length=280, Percent_Identity=26.4285714285714, Blast_Score=66, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17507723, Length=190, Percent_Identity=25.2631578947368, Blast_Score=65, Evalue=7e-11,
Organism=Saccharomyces cerevisiae, GI6319493, Length=358, Percent_Identity=24.3016759776536, Blast_Score=72, Evalue=2e-13,
Organism=Drosophila melanogaster, GI21356223, Length=341, Percent_Identity=26.099706744868, Blast_Score=92, Evalue=4e-19,
Organism=Drosophila melanogaster, GI19923002, Length=367, Percent_Identity=23.433242506812, Blast_Score=66, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005888
- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.46 [H]

Molecular weight: Translated: 39631; Mature: 39631

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKSAILITGGAGFIGSNFIHYFMDVYPEVPIVNIDKLTYAGSLQNLKGIDSNSNYHFVE
CCCCEEEEECCCCHHHHHHHHHHHHHCCCCCEEEECCEEECCCHHHCCCCCCCCCEEEEE
GDIADEQVVQHVFQNFDIHGVIHFAAESHVDRSIEDARSFVDTNVVGTLNLLQAARNDWE
CCCCHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCHH
EKGELEGRRFHHISTDEVYGSLGEEGMFTEETPYDPRNPYSASKAGANLMVKSFGYTYGM
HHCCCCCCEEECCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHCCCHHHHHHCCCCCCC
NVILSSSSNNYGPRQHQEKLLPTIIEKALSGKEIPIYGDGKNVRDWLYVEDHCRAIDTVY
EEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCEEEEHHHHHHHHHHH
HFGKSQETYNVGGRNERTNLEMTNQICDILNDLRPDLLSKYELEKFQDLITFVEDRRGHD
HCCCCCCEECCCCCCCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCC
LRYAIDDRKLRNELQWEPKQTLENGLKQTIEWYIRKWESPTKMNQ
EEEEECHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MNKSAILITGGAGFIGSNFIHYFMDVYPEVPIVNIDKLTYAGSLQNLKGIDSNSNYHFVE
CCCCEEEEECCCCHHHHHHHHHHHHHCCCCCEEEECCEEECCCHHHCCCCCCCCCEEEEE
GDIADEQVVQHVFQNFDIHGVIHFAAESHVDRSIEDARSFVDTNVVGTLNLLQAARNDWE
CCCCHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCHH
EKGELEGRRFHHISTDEVYGSLGEEGMFTEETPYDPRNPYSASKAGANLMVKSFGYTYGM
HHCCCCCCEEECCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHCCCHHHHHHCCCCCCC
NVILSSSSNNYGPRQHQEKLLPTIIEKALSGKEIPIYGDGKNVRDWLYVEDHCRAIDTVY
EEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCEEEEHHHHHHHHHHH
HFGKSQETYNVGGRNERTNLEMTNQICDILNDLRPDLLSKYELEKFQDLITFVEDRRGHD
HCCCCCCEECCCCCCCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCC
LRYAIDDRKLRNELQWEPKQTLENGLKQTIEWYIRKWESPTKMNQ
EEEEECHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7961452 [H]