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The map label for this gene is aprX [H]

Identifier: 23099830

GI number: 23099830

Start: 2426285

End: 2427577

Strand: Reverse

Name: aprX [H]

Synonym: OB2375

Alternate gene names: 23099830

Gene position: 2427577-2426285 (Counterclockwise)

Preceding gene: 23099831

Following gene: 23099829

Centisome position: 66.87

GC content: 36.43

Gene sequence:

>1293_bases
ATGTTAAATTTTTCAATGATTAATTTGGTTCGTCGTAATGGTAAGAAGATGGACAAAGGGATCAGACAAGAAATGGTGAA
TTTCTATAAACCGTTTCGTTATATTCCTTGTTTTTTACAAAGTCCGATAGAACGAATTAAAAAGAAAACAAAAAAATTAC
CTGTTGTTATTAAATTCAAATCAGACAATTTTGCTAATGGATTACATGAAGTGAAAAATACAAAATGTAAATCGCTTCGT
GAATTTCATTCTATTTCCTGTTGCTCAGCTAAATTTTCTGCAGAAAAAATTGAGCAATTAATTGAAAATTGCAGCCTAAT
TGAGAAAATCTATTATGATAGAAAGGTTTTTTCATTACTAGATACTGCTTCTTCATCAATAAATGCTGATGTATTAAAGG
AGAGTGGGCTGACAGGACAAGGGTCTACTATTGCGGTTATCGATACAGGAATACATCCGCATGAGGATTTAGAAGGTCGT
ATTATTGGGTTTGCTGATTTCGTAAAAGGGCAAACAGAACCTTATGATGATAATGGACATGGTACACACTGTGCTGGAGA
TGCTGCTGGTAATGGTGCATTGTCGGATGGGCAATATCAGGGGCCAGCGCCAGACGCAAATTTAGTCGGTGTAAAAGTGT
TAAATAAAACTGGTTCAGGGTCATTATCTACGGTTATAGAAGGTATTGATTGGTGTATTCAAAATCAGTCTAAATATAAT
ATAAATATACTATCCCTATCACTTGGTTCAGACGCTACAGAGCCTGCTGAAGGTGATCCTGTTGTTAATGCTGTAGAAAC
GGCTTGGGATAATGGTATGGTTGTATGTGTTGCTGCTGGTAATTCTGGTCCTGGAGATAAGACAGTAGGCAGTCCTGGAA
TAAGTCCTAAAGTAATAACTGTTGGTGCTGCAGATGATAATAATACCGCTGAACGTTCTGACGATTCAGTGGCTGAATTT
TCTAGTAGGGGACCTACCATTGATGGTTTAACAAAACCAAATTTATTAACGCCAGGCGTTGATATCGTATCCTTAAGAGC
ACCAGGTTCTTTCATTGATAAAACAAATAAATCAGCTCGTGTTGGAAGCAATTATATCTCTTTATCTGGTACTTCCATGG
CGACTCCAATTTGCGCAGGTATAGTTGCTCAATTACTTCAAAGTGATTCAAGCTTAACTCCTAATCAAGTAAAGGAAAAA
TTAATGGAAGCATGCCAAGATTTAGGACAATCCCCTAATGTTCAAGGAGCAGGGTATCTAAATGCTGCCAATTTAATTAA
TATAAATGAATAG

Upstream 100 bases:

>100_bases
ATAGATTTCTACCTCTTTTTCTATTTTTTATACTATTTCACTACTCTCTTTTTACAAAATTTGCGTACATTATCGTAAAT
CAATGAGAGGGAGAAATTAT

Downstream 100 bases:

>100_bases
TATCTTGAGATAGTGATAAAGCATAACCATTCATGTAACTAGATAACTAGAACACCTCCAACATACTAAGAATAGTGGGC
AAAATCATCCGTGGATGATC

Product: intracellular alkaline serine proteinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 430; Mature: 430

Protein sequence:

>430_residues
MLNFSMINLVRRNGKKMDKGIRQEMVNFYKPFRYIPCFLQSPIERIKKKTKKLPVVIKFKSDNFANGLHEVKNTKCKSLR
EFHSISCCSAKFSAEKIEQLIENCSLIEKIYYDRKVFSLLDTASSSINADVLKESGLTGQGSTIAVIDTGIHPHEDLEGR
IIGFADFVKGQTEPYDDNGHGTHCAGDAAGNGALSDGQYQGPAPDANLVGVKVLNKTGSGSLSTVIEGIDWCIQNQSKYN
INILSLSLGSDATEPAEGDPVVNAVETAWDNGMVVCVAAGNSGPGDKTVGSPGISPKVITVGAADDNNTAERSDDSVAEF
SSRGPTIDGLTKPNLLTPGVDIVSLRAPGSFIDKTNKSARVGSNYISLSGTSMATPICAGIVAQLLQSDSSLTPNQVKEK
LMEACQDLGQSPNVQGAGYLNAANLININE

Sequences:

>Translated_430_residues
MLNFSMINLVRRNGKKMDKGIRQEMVNFYKPFRYIPCFLQSPIERIKKKTKKLPVVIKFKSDNFANGLHEVKNTKCKSLR
EFHSISCCSAKFSAEKIEQLIENCSLIEKIYYDRKVFSLLDTASSSINADVLKESGLTGQGSTIAVIDTGIHPHEDLEGR
IIGFADFVKGQTEPYDDNGHGTHCAGDAAGNGALSDGQYQGPAPDANLVGVKVLNKTGSGSLSTVIEGIDWCIQNQSKYN
INILSLSLGSDATEPAEGDPVVNAVETAWDNGMVVCVAAGNSGPGDKTVGSPGISPKVITVGAADDNNTAERSDDSVAEF
SSRGPTIDGLTKPNLLTPGVDIVSLRAPGSFIDKTNKSARVGSNYISLSGTSMATPICAGIVAQLLQSDSSLTPNQVKEK
LMEACQDLGQSPNVQGAGYLNAANLININE
>Mature_430_residues
MLNFSMINLVRRNGKKMDKGIRQEMVNFYKPFRYIPCFLQSPIERIKKKTKKLPVVIKFKSDNFANGLHEVKNTKCKSLR
EFHSISCCSAKFSAEKIEQLIENCSLIEKIYYDRKVFSLLDTASSSINADVLKESGLTGQGSTIAVIDTGIHPHEDLEGR
IIGFADFVKGQTEPYDDNGHGTHCAGDAAGNGALSDGQYQGPAPDANLVGVKVLNKTGSGSLSTVIEGIDWCIQNQSKYN
INILSLSLGSDATEPAEGDPVVNAVETAWDNGMVVCVAAGNSGPGDKTVGSPGISPKVITVGAADDNNTAERSDDSVAEF
SSRGPTIDGLTKPNLLTPGVDIVSLRAPGSFIDKTNKSARVGSNYISLSGTSMATPICAGIVAQLLQSDSSLTPNQVKEK
LMEACQDLGQSPNVQGAGYLNAANLININE

Specific function: Displays serine protease activity. Seems to have a broad substrate specificity [H]

COG id: COG1404

COG function: function code O; Subtilisin-like serine proteases

Gene ontology:

Cell location: Cytoplasm (Probable). Note=Is leaked to the culture medium during the late stationary phase owing to cell lysis [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S8 family [H]

Homologues:

Organism=Homo sapiens, GI4506775, Length=331, Percent_Identity=29.3051359516616, Blast_Score=88, Evalue=2e-17,
Organism=Homo sapiens, GI186972143, Length=249, Percent_Identity=28.5140562248996, Blast_Score=67, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17533391, Length=265, Percent_Identity=27.9245283018868, Blast_Score=67, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6319893, Length=261, Percent_Identity=36.7816091954023, Blast_Score=114, Evalue=4e-26,
Organism=Saccharomyces cerevisiae, GI6324576, Length=331, Percent_Identity=32.9305135951662, Blast_Score=109, Evalue=8e-25,
Organism=Saccharomyces cerevisiae, GI6320775, Length=286, Percent_Identity=35.3146853146853, Blast_Score=109, Evalue=9e-25,
Organism=Drosophila melanogaster, GI45550681, Length=312, Percent_Identity=32.6923076923077, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24653270, Length=264, Percent_Identity=29.1666666666667, Blast_Score=70, Evalue=3e-12,
Organism=Drosophila melanogaster, GI17137360, Length=264, Percent_Identity=29.1666666666667, Blast_Score=70, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000209
- InterPro:   IPR022398
- InterPro:   IPR015500 [H]

Pfam domain/function: PF00082 Peptidase_S8 [H]

EC number: NA

Molecular weight: Translated: 45839; Mature: 45839

Theoretical pI: Translated: 5.73; Mature: 5.73

Prosite motif: PS00136 SUBTILASE_ASP ; PS00137 SUBTILASE_HIS ; PS00138 SUBTILASE_SER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNFSMINLVRRNGKKMDKGIRQEMVNFYKPFRYIPCFLQSPIERIKKKTKKLPVVIKFK
CCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEC
SDNFANGLHEVKNTKCKSLREFHSISCCSAKFSAEKIEQLIENCSLIEKIYYDRKVFSLL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
DTASSSINADVLKESGLTGQGSTIAVIDTGIHPHEDLEGRIIGFADFVKGQTEPYDDNGH
HHHCCCCCHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCCEEEHHHHHCCCCCCCCCCCC
GTHCAGDAAGNGALSDGQYQGPAPDANLVGVKVLNKTGSGSLSTVIEGIDWCIQNQSKYN
CCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCEE
INILSLSLGSDATEPAEGDPVVNAVETAWDNGMVVCVAAGNSGPGDKTVGSPGISPKVIT
EEEEEEECCCCCCCCCCCCHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCEEEE
VGAADDNNTAERSDDSVAEFSSRGPTIDGLTKPNLLTPGVDIVSLRAPGSFIDKTNKSAR
EECCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEECCCHHHHCCCCCCC
VGSNYISLSGTSMATPICAGIVAQLLQSDSSLTPNQVKEKLMEACQDLGQSPNVQGAGYL
CCCCEEEECCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEE
NAANLININE
CCCCEEEECC
>Mature Secondary Structure
MLNFSMINLVRRNGKKMDKGIRQEMVNFYKPFRYIPCFLQSPIERIKKKTKKLPVVIKFK
CCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEC
SDNFANGLHEVKNTKCKSLREFHSISCCSAKFSAEKIEQLIENCSLIEKIYYDRKVFSLL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
DTASSSINADVLKESGLTGQGSTIAVIDTGIHPHEDLEGRIIGFADFVKGQTEPYDDNGH
HHHCCCCCHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCCEEEHHHHHCCCCCCCCCCCC
GTHCAGDAAGNGALSDGQYQGPAPDANLVGVKVLNKTGSGSLSTVIEGIDWCIQNQSKYN
CCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCEE
INILSLSLGSDATEPAEGDPVVNAVETAWDNGMVVCVAAGNSGPGDKTVGSPGISPKVIT
EEEEEEECCCCCCCCCCCCHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCEEEE
VGAADDNNTAERSDDSVAEFSSRGPTIDGLTKPNLLTPGVDIVSLRAPGSFIDKTNKSAR
EECCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEECCCHHHHCCCCCCC
VGSNYISLSGTSMATPICAGIVAQLLQSDSSLTPNQVKEKLMEACQDLGQSPNVQGAGYL
CCCCEEEECCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEE
NAANLININE
CCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]